The Experts below are selected from a list of 6279 Experts worldwide ranked by ideXlab platform
Alfred Böcking - One of the best experts on this subject based on the ideXlab platform.
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equivocal cytology in lung cancer diagnosis improvement of diagnostic accuracy using adjuvant multicolor fish dna Image Cytometry and quantitative promoter hypermethylation analysis
Cancer Cytopathology, 2011Co-Authors: Martin Schramm, Natalia Pomjanski, Christian Wrobel, Ingmar Born, Marietta Kazimirek, Marina William, Rainer Kappes, Claus Dieter Gerharz, Stefan Biesterfeld, Alfred BöckingAbstract:BACKGROUND: Sometimes, cytological lung cancer diagnosis is challenging because equivocal diagnoses are common. To enhance diagnostic accuracy, fluorescent in situ hybridization (FISH), DNA-Image Cytometry, and quantitative promoter hypermethylation analysis have been proposed as adjuncts. METHODS: Bronchial washings and/or brushings or transbronchial fine-needle aspiration biopsies were prospectively collected from patients who were clinically suspected of having lung carcinoma. After routine cytological diagnosis, 70 consecutive specimens, each cytologically diagnosed as negative, equivocal, or positive for cancer cells, were investigated with adjuvant methods. Suspicious areas on the smears were restained with the LAVysion multicolor FISH probe set (Abbott Molecular, Des Plaines, Illinois) or according to the Feulgen Staining Method for DNA-Image Cytometry analysis. DNA was extracted from residual liquid material, and frequencies of aberrant methylation of APC, p16INK4A, and RASSF1A gene promoters were determined with quantitative methylation-specific polymerase chain reaction (QMSP) after bisulfite conversion. Clinical and histological follow-up according to a reference standard, defined in advance, were available for 198 of 210 patients. RESULTS: In the whole cohort, cytology, FISH, DNA-Image Cytometry, and QMSP achieved sensitivities of 83.7%, 78%, 79%, and 49.6%, respectively (specificities of 69.8%, 98.2%, 98.2%, and 98.4%, respectively). Subsequent to cytologically equivocal diagnoses, FISH, DNA-Image Cytometry, and QMSP definitely identified malignancy in 79%, 83%, and 49%, respectively. With QMSP, 4 of 22 cancer patients with cytologically negative diagnoses were correctly identified. CONCLUSIONS: Thus, adjuvant FISH or DNA-Image Cytometry in cytologically equivocal diagnoses improves diagnostic accuracy at comparable rates. Adjuvant QMSP in cytologically negative cases with persistent suspicion of lung cancer would enhance sensitivity. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society.
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Additional use of DNA‐Image Cytometry improves the assessment of resection margins
Journal of Oral Pathology & Medicine, 2007Co-Authors: Jörg Handschel, Natalia Pomjanski, Rita Depprich, Michelle A. Ommerborn, Stefan Braunstein, Norbert R. Kübler, Ulrich Meyer, Alfred BöckingAbstract:Background: Despite the histopathologic findings of tumor-free margins, patients with oral squamous cell carcinoma (SCC) often suffer from local tumor relapse. The purpose of this study was to determine the prognostic value of DNA-Image Cytometry in the assessment of resection margins. Methods: DNA-Image Cytometry was performed in 40 SCC patients with histologically tumor-free resection margins. The follow-up period since the tumor resection was at least 3 years. Results: Twenty patients showed a locoregional relapse of the SCC. Fourteen of these patients had aneuploid cells in DNA-Image Cytometry. Two patients who were relapse-free revealed aneuploid cells too. The sensitivity of the adjuvant use of DNA-Image Cytometry was 70% and the positive predictive value was 87.5%. Conclusions: The additional use of DNA-Image Cytometry is a reasonable tool for the assessment of the resection margins of SCCs. DNA-Image Cytometry could help to find the appropriate treatment option for the patients and thus might improve their prognosis.
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Diagnostic DNA-flow- vs. -Image-Cytometry in effusion cytology.
Analytical Cellular Pathology, 2002Co-Authors: Motherby H, Natalia Pomjanski, Mary Kube, Alexandra Boros, Thomas Heiden, Bernhard Tribukait, Alfred BöckingAbstract:Aims: To determine the sensitivity and specificity of flow‐ and Image‐Cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions. Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐Image‐Cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐Image‐Cytometry, respectively [7,23,29,49]. Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐Image‐Cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐Image‐Cytometry. Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions Image‐Cytometry revealed superior sensitivity as compared with monoparametric flow Cytometry.
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Diagnosis of bladder cancer with urinary cytology, immunocytology and DNA-Image-Cytometry.
Analytical Cellular Pathology, 2001Co-Authors: Bernhard Planz, Alfred Böcking, Christian Synek, Thomas Deix, Michael MarbergerAbstract:DNA‐Image‐Cytometry and antibodies directed against the Lewis X‐ and the 486p 3/12 antigen were applied to improve diagnostic accuracy of urinary cytology for the detection of bladder cancer. Cytology, immunocytology and DNA‐Image‐Cytometry were performed in spontaneously voided urine samples and barbotage bladder washings from 71 patients. The DNA content was determined using the CM‐1 Cytometer according to the recommendation of the ESCAP Consensus Report on Standardization of DNA‐Image‐Cytometry (1995). For immunocytological examination we used the monoclonal anti Lewis X antibody P‐12 and antibody 486p 3/12. All patients underwent subsequent cystoscopy and for any suspicious lesion biopsy or transurethral resection was done. Histological findings revealed 31 patients with transitional cell carcinomas of different stages and grades of malignancy. 40 patients had various benign diseases of the urinary bladder. Cytology yielded a sensitivity of 68% and a specificity of 100%. DNA aneuploidy was detected in 81% of cancer patients with a specificity of 100%. By combination of these two methods the overall sensitivity increased to 87%. Immunocytology with Lewis X and 486p 3/12 antibodies showed reactivity in 84% and 87% in combination with a specificity of 80% and 70%, respectively. By combining urinary cytology, immunocytology and/or DNA‐Image‐Cytometry the overall sensitivity increased to 94% with no change in specificity. DNA‐Image‐Cytometry should be used to evaluate particularly urothelial cells suspicious for malignancy in urinary specimens. Because of low specificity the monoclonal antibodies against Lewis X‐ and 486p 3/12 antigens are not helpful in screening for bladder cancer. Nevertheless, their high sensitivity may justify their use in case DNA Image Cytometry is not available and in the follow up of patients with transitional cell carcinoma.
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Fourth updated ESACP consensus report on diagnostic DNA Image Cytometry.
Analytical Cellular Pathology, 2001Co-Authors: Gunter Haroske, J. P. Baak, Håvard E. Danielsen, F. Giroud, A. Gschwendtner, Martin Oberholzer, Albrecht Reith, P. Spieler, Alfred BöckingAbstract:A task force of experts in the field of diagnostic DNA Image Cytometry, invited by the ESACP, and further scientists or physicians revealing experience in that diagnostic procedure (names are given in Addendum A), agreed upon the following 4th updated Consensus Report on Standardised Diagnostic DNA Image Cytometry during the 7th International Congress of that society in Caen, 2001. This report is based on the three preceding ones [6,14,17]. It deals with the following items: – Critical review and update of the definitions given in the 1997 Consensus Update; – Review and detailed description of basic terms, principles and algorithms for diagnostic interpretation; – Recommendations concerning diagnostic or prognostic applications in specific fields of tumour pathology. This update is not aimed to substitute the 1997 consensus, but to make necessary addenda and give more detailed descriptions of those items not unequivocally to interpret by potential users of the methodology.
Randall J Kimple - One of the best experts on this subject based on the ideXlab platform.
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high throughput detection of dna double strand breaks using Image Cytometry
BioTechniques, 2015Co-Authors: Tyler L Fowler, Alison M Bailey, B Bednarz, Randall J KimpleAbstract:Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated Image Cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.
Peter W. Hamilton - One of the best experts on this subject based on the ideXlab platform.
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Whole slide Image Cytometry: a novel method to detect abnormal DNA content in Barrett’s esophagus
Laboratory Investigation, 2015Co-Authors: Yinhai Wang, Damian T. Mcmanus, Kenneth Arthur, Brian T. Johnston, Andrew Kennedy, Helen G. Coleman, Liam J. Murray, Peter W. HamiltonAbstract:Barrett’s esophagus (BE) is a precursor of esophageal adenocarcinoma (EAC). Both low-grade dysplasia (LGD) and high-grade dysplasia (HGD) are associated with an increased risk of progression to EAC. However, histological interpretation and grading of dysplasia (particularly LGD) is subjective and poorly reproducible. This study has combined whole slide imaging with DNA Image Cytometry to provide a novel method for the detection of abnormal DNA content through Image analysis of tissue sections. A total of 20 cases were evaluated, including 8 negative for dysplasia (NFD), 6 LGD, and 6 HGD. Feulgen-stained esophageal sections were scanned in their entirety. Barrett’s mucosa was interactively chosen for automatic nuclei segmentation where irrelevant cell types were ignored. The combined DNA content histogram for all nuclei within selected Image regions was then obtained. In addition, three histogram measurements were computed, including xER-5C, 2cDI, and DNA-MG. Visual evaluation suggested the shape of DNA content histograms from NFD, LGD, and HGD cases exhibiting identifiable differences. The histogram measurements, xER-5C, 2cDI, and DNA-MG, were shown to be effective in differentiating metaplastic from dysplastic cases with statistical significance. Moreover, they also successfully separated NFD, LGD, and HGD patients with statistical significance. Whole slide Image Cytometry is a novel and effective method for the detection of abnormal DNA content in BE. Compared with histological review, it is more objective. Compared with flow Cytometry and cytology-preparation Image Cytometry, it is low cost, simple to use, only requires a single 1 μm section, and facilitates selection of tissue and topographical correlation. Whole slide Image Cytometry can detect differences in DNA content between NFD, LGD, and HGD patients in this cross-sectional study. Abnormal DNA content detection by whole slide Image Cytometry is a promising biomarker of progression that could affect future diagnostics in BE.
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whole slide Image Cytometry a novel method to detect abnormal dna content in barrett s esophagus
Laboratory Investigation, 2015Co-Authors: Yinhai Wang, Damian T. Mcmanus, Kenneth Arthur, Brian T. Johnston, Andrew Kennedy, Helen G. Coleman, Liam J. Murray, Peter W. HamiltonAbstract:Barrett’s esophagus (BE) is a precursor of esophageal adenocarcinoma (EAC). Both low-grade dysplasia (LGD) and high-grade dysplasia (HGD) are associated with an increased risk of progression to EAC. However, histological interpretation and grading of dysplasia (particularly LGD) is subjective and poorly reproducible. This study has combined whole slide imaging with DNA Image Cytometry to provide a novel method for the detection of abnormal DNA content through Image analysis of tissue sections. A total of 20 cases were evaluated, including 8 negative for dysplasia (NFD), 6 LGD, and 6 HGD. Feulgen-stained esophageal sections were scanned in their entirety. Barrett’s mucosa was interactively chosen for automatic nuclei segmentation where irrelevant cell types were ignored. The combined DNA content histogram for all nuclei within selected Image regions was then obtained. In addition, three histogram measurements were computed, including xER-5C, 2cDI, and DNA-MG. Visual evaluation suggested the shape of DNA content histograms from NFD, LGD, and HGD cases exhibiting identifiable differences. The histogram measurements, xER-5C, 2cDI, and DNA-MG, were shown to be effective in differentiating metaplastic from dysplastic cases with statistical significance. Moreover, they also successfully separated NFD, LGD, and HGD patients with statistical significance. Whole slide Image Cytometry is a novel and effective method for the detection of abnormal DNA content in BE. Compared with histological review, it is more objective. Compared with flow Cytometry and cytology-preparation Image Cytometry, it is low cost, simple to use, only requires a single 1 μm section, and facilitates selection of tissue and topographical correlation. Whole slide Image Cytometry can detect differences in DNA content between NFD, LGD, and HGD patients in this cross-sectional study. Abnormal DNA content detection by whole slide Image Cytometry is a promising biomarker of progression that could affect future diagnostics in BE.
Tyler L Fowler - One of the best experts on this subject based on the ideXlab platform.
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high throughput detection of dna double strand breaks using Image Cytometry
BioTechniques, 2015Co-Authors: Tyler L Fowler, Alison M Bailey, B Bednarz, Randall J KimpleAbstract:Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated Image Cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.
Yinhai Wang - One of the best experts on this subject based on the ideXlab platform.
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Whole slide Image Cytometry: a novel method to detect abnormal DNA content in Barrett’s esophagus
Laboratory Investigation, 2015Co-Authors: Yinhai Wang, Damian T. Mcmanus, Kenneth Arthur, Brian T. Johnston, Andrew Kennedy, Helen G. Coleman, Liam J. Murray, Peter W. HamiltonAbstract:Barrett’s esophagus (BE) is a precursor of esophageal adenocarcinoma (EAC). Both low-grade dysplasia (LGD) and high-grade dysplasia (HGD) are associated with an increased risk of progression to EAC. However, histological interpretation and grading of dysplasia (particularly LGD) is subjective and poorly reproducible. This study has combined whole slide imaging with DNA Image Cytometry to provide a novel method for the detection of abnormal DNA content through Image analysis of tissue sections. A total of 20 cases were evaluated, including 8 negative for dysplasia (NFD), 6 LGD, and 6 HGD. Feulgen-stained esophageal sections were scanned in their entirety. Barrett’s mucosa was interactively chosen for automatic nuclei segmentation where irrelevant cell types were ignored. The combined DNA content histogram for all nuclei within selected Image regions was then obtained. In addition, three histogram measurements were computed, including xER-5C, 2cDI, and DNA-MG. Visual evaluation suggested the shape of DNA content histograms from NFD, LGD, and HGD cases exhibiting identifiable differences. The histogram measurements, xER-5C, 2cDI, and DNA-MG, were shown to be effective in differentiating metaplastic from dysplastic cases with statistical significance. Moreover, they also successfully separated NFD, LGD, and HGD patients with statistical significance. Whole slide Image Cytometry is a novel and effective method for the detection of abnormal DNA content in BE. Compared with histological review, it is more objective. Compared with flow Cytometry and cytology-preparation Image Cytometry, it is low cost, simple to use, only requires a single 1 μm section, and facilitates selection of tissue and topographical correlation. Whole slide Image Cytometry can detect differences in DNA content between NFD, LGD, and HGD patients in this cross-sectional study. Abnormal DNA content detection by whole slide Image Cytometry is a promising biomarker of progression that could affect future diagnostics in BE.
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whole slide Image Cytometry a novel method to detect abnormal dna content in barrett s esophagus
Laboratory Investigation, 2015Co-Authors: Yinhai Wang, Damian T. Mcmanus, Kenneth Arthur, Brian T. Johnston, Andrew Kennedy, Helen G. Coleman, Liam J. Murray, Peter W. HamiltonAbstract:Barrett’s esophagus (BE) is a precursor of esophageal adenocarcinoma (EAC). Both low-grade dysplasia (LGD) and high-grade dysplasia (HGD) are associated with an increased risk of progression to EAC. However, histological interpretation and grading of dysplasia (particularly LGD) is subjective and poorly reproducible. This study has combined whole slide imaging with DNA Image Cytometry to provide a novel method for the detection of abnormal DNA content through Image analysis of tissue sections. A total of 20 cases were evaluated, including 8 negative for dysplasia (NFD), 6 LGD, and 6 HGD. Feulgen-stained esophageal sections were scanned in their entirety. Barrett’s mucosa was interactively chosen for automatic nuclei segmentation where irrelevant cell types were ignored. The combined DNA content histogram for all nuclei within selected Image regions was then obtained. In addition, three histogram measurements were computed, including xER-5C, 2cDI, and DNA-MG. Visual evaluation suggested the shape of DNA content histograms from NFD, LGD, and HGD cases exhibiting identifiable differences. The histogram measurements, xER-5C, 2cDI, and DNA-MG, were shown to be effective in differentiating metaplastic from dysplastic cases with statistical significance. Moreover, they also successfully separated NFD, LGD, and HGD patients with statistical significance. Whole slide Image Cytometry is a novel and effective method for the detection of abnormal DNA content in BE. Compared with histological review, it is more objective. Compared with flow Cytometry and cytology-preparation Image Cytometry, it is low cost, simple to use, only requires a single 1 μm section, and facilitates selection of tissue and topographical correlation. Whole slide Image Cytometry can detect differences in DNA content between NFD, LGD, and HGD patients in this cross-sectional study. Abnormal DNA content detection by whole slide Image Cytometry is a promising biomarker of progression that could affect future diagnostics in BE.
