The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Subhash Bhatia - One of the best experts on this subject based on the ideXlab platform.
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biosorption of cadmium ii ions by Immobilized Cells of pycnoporus sanguineus from aqueous solution
Bioresource Technology, 2008Co-Authors: M D Mashitah, Yus Y Azila, Subhash BhatiaAbstract:Abstract Biosorption of cadmium (II) ions from aqueous solution onto Immobilized Cells of Pycnoporus sanguineus ( P. sanguineus ) was investigated in a batch system. Equilibrium and kinetic studies were conducted by considering the effect of pH, initial cadmium (II) concentration, biomass loading and temperature. Results showed that the uptake of cadmium (II) ions increased with the increase of initial cadmium (II) concentration, pH and temperature. Langmuir, Freundlich and Redlich–Peterson isotherm models were used to analyze the equilibrium data at different temperatures. Langmuir isotherm model described the experimental data well followed by Redlich–Peterson and Freundlich isotherm models. Biosorption kinetics data were fitted using pseudo-first, pseudo-second-order and intraparticle diffusion. It was found that the kinetics data fitted well the pseudo-second-order followed by intraparticle diffusion. Thermodynamic parameters such as standard Gibbs free energy (Δ G 0 ), standard enthalpy (Δ H 0 ) and standard entropy (Δ S 0 ) were evaluated. The result showed that biosorption of cadmium (II) ions onto Immobilized Cells of P. sanguineus was spontaneous and endothermic nature.
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Biosorption of copper (II) onto Immobilized Cells of Pycnoporus sanguineus from aqueous solution: Equilibrium and kinetic studies
Journal of hazardous materials, 2008Co-Authors: Yus Azila Yahaya, Mashitah Mat Don, Subhash BhatiaAbstract:Abstract The ability of white-rot fungus, Pycnoporus sanguineus to adsorb copper (II) ions from aqueous solution is investigated in a batch system. The live fungus Cells were Immobilized into Ca-alginate gel to study the influence of pH, initial metal ions concentration, biomass loading and temperature on the biosorption capacity. The optimum uptake of Cu (II) ions was observed at pH 5 with a value of 2.76 mg/g. Biosorption equilibrium data were best described by Langmuir isotherm model followed by Redlich–Peterson and Freundlich models, respectively. The biosorption kinetics followed the pseudo-second order and intraparticle diffusion equations. The thermodynamic parameters enthalpy change (10.16 kJ/mol) and entropy change (33.78 J/mol K) were determined from the biosorption equilibrium data. The FTIR analysis showed that OH, NH, C H, C O, COOH and C N groups were involved in the biosorption of Cu (II) ions onto Immobilized Cells of P. sanguineus . The Immobilized Cells of P. sanguineus were capable of removing Cu (II) ions from aqueous solution.
Paramasamy Gunasekaran - One of the best experts on this subject based on the ideXlab platform.
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Production of ethanol from liquefied cassava starch using co-Immobilized Cells of Zymomonas mobilis and Saccharomyces diastaticus.
Journal of bioscience and bioengineering, 2001Co-Authors: Ramasamy Amutha, Paramasamy GunasekaranAbstract:Co-Immobilized Cells of Saccharomyces diastaticus and Zymomonas mobilis produced a high ethanol concentration compared to Immobilized Cells of S. diastaticus during batch fermentation of liquefied cassava starch. The co-Immobilized Cells produced 46.7 g/l ethanol from 150 g/l liquefied cassava starch, while Immobilized Cells of yeast S. diastaticus produced 37.5 g/l ethanol. The concentration of ethanol produced by Immobilized Cells was higher than that by free Cells of S. diastaticus and Z. mobilis in mixed-culture fermentation. In repeated-batch fermentation using co-Immobilized Cells, the ethanol concentration increased to 53.5 g/l. The co-Immobilized gel beads were stable up to seven successive batches. Continuous fermentation using co-Immobilized Cells in a packed bed column reactor operated at a flow rate of 15 ml/h (residence time, 4 h) exhibited a maximum ethanol productivity of 8.9 g/l/h.
A.m. Delort - One of the best experts on this subject based on the ideXlab platform.
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2-Aminobenzothiazole degradation by free and Ca-alginate Immobilized Cells of Rhodococcus rhodochrous
Chemosphere, 2009Co-Authors: Charlène Chorao, Franck Charmantray, Pascale Besse-hoggan, Martine Sancelme, Angela Cincilei, Mounir Traïkia, Gilles Mailhot, A.m. DelortAbstract:2-Aminobenzothiazole (ABT) degradation was investigated using free and Immobilized systems during photodegradation under solar light in the presence of Fe(III)-nitrilotriacetic acid (FeNTA), biodegradation by Rhodococcus rhodochrous, and during combined conditions. Ca-alginate hydrogel was chosen as a model matrix and some complementary studies were required to characterize this new system. R. rhodochrous metabolism in this type of environment was monitored by NMR spectroscopy. Neither change in intracellular pH values nor in ATP concentrations was observed by in vivo 31P NMR, showing that no metabolic modification occurred between free and Immobilized Cells. 1H NMR demonstrated that alginate was not used as carbon source by R. rhodochrous. After establishing the pre-treatment protocol by SPE to eliminate solubilised alginate, ABT adsorption on beads and degradation were studied. The same pathways of transformation were observed in suspended and Immobilized cell systems. Considering the ABT adsorption phenomenon on alginate beads (8%), the efficiency of the two systems was found to be comparable although the degradation rate was slightly lower with Immobilized Cells. The most important result was the finding that the positive effect of FeNTA on ABT degradation with Immobilized Cells was similar to that observed previously with free Cells. All these results show that mechanisms observed with free Cells can be extrapolated to entrapped Cells, i.e. under conditions much closer to those usually encountered in the environment.
Jue Wang - One of the best experts on this subject based on the ideXlab platform.
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Study on Immobilized Cells for producing alpha-amylase by using polyving alcohol as the carrier(II): The effect of fermentating conditions on the ability producing alpha-amylase of the Cells Immobilized with polyving alcohol as the corrier and contin
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 1998Co-Authors: Liu Z, Jue WangAbstract:The effects of fermentating conditions on the ability of Immobilized Cells with PVA as carrier for producing alpha-amylase were studied. The continuous fermentation with the Immobilized Cells were tested in continuous flow stirred tank reactor (CSTR). The results showed that the adaptability of the Immobilized Bacillus substilis to pH increased after immobilization. In CSTR, the Immobilized Cells can be fermentated continuously for 360 hrs and the activity of alpha-amylase can be kept on the level of about 170 u/ml.
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Study on Immobilized Cells for producing alpha-amylase by using polyvinyl alcohol as the carrier (I)
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 1997Co-Authors: Liu Z, Jue WangAbstract:The method of immobilization of Bacillus subtilis by using polyvingyl alcohol(PVA) as the carrier of Immobilized Cells was studied. The effects of conditions of immobilization on the structure and mechanical strength of PVA Immobilized Cells and the ability for producing alpha-amylase of the Immobilized Cells were investigated. The comparative test of fermentation by using the Immobilized Cells with PVA as carrier and the Immobilized Cells with sodium glyinate as carrier and free Cells was carried out in the same condition. The results showed that Bacillus substilis Cells were Immobilized efficently by PVA and the Immobilized Cells could normally grow and reproduce and produce alpha-amylase. In the same condition, the activity of alpha-amylase produced boy the Immobilized Cells was 28% higher than that produced by free Cells and 11% higher than that produced by the Cells Immobilized with sodium glyinate as carrier.
Harichandra Z. Ninnekar - One of the best experts on this subject based on the ideXlab platform.
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Biodegradation of cypermethrin by Immobilized Cells of Micrococcus sp. strain CPN 1
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2015Co-Authors: Preeti N. Tallur, Sikandar I. Mulla, Veena B. Megadi, Manjunatha P. Talwar, Harichandra Z. NinnekarAbstract:Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by Immobilized Cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by Immobilized Cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended Cells were compared with that of Immobilized Cells in batches and semi-continuous with shaken cultures. PUF-Immobilized Cells showed higher degradation of cypermethrin (10 mM and 20 mM) than freely suspended Cells and Cells Immobilized in other matrices. The PUF-Immobilized Cells of Micrococcus sp. strain CPN 1 were retain their degradation capacity. Thus, they can be reused for more than 32 cycles, without losing their degradation capacity. Hence, the PUF-Immobilized Cells of Micrococcus sp. could potentially be used in the bioremediation of cypermethrin contaminated water.
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Enhanced degradation of 3-nitrobenzoate by Immobilized Cells of Bacillus flexus strain XJU-4
Biotechnology and Bioprocess Engineering, 2012Co-Authors: Sikandar I. Mulla, Manjunatha P. Talwar, Robertcyril S. Hoskeri, Harichandra Z. NinnekarAbstract:Nitroaromatic compounds are major chemical pollutants because of their widespread use and toxicity. Bioremediation of such toxic nitroaromatic compounds using microorganisms may provide an effective method for detoxification. Bacillus flexus strain XJU-4, capable of degrading 3-nitrobenzoate, was Immobilized in various matrices, namely polyurethane foam (PUF), polyacrylamide, sodium alginate (SA), sodium alginate-polyvinyl alcohol (SA-PVA) and agar. The degradation of 12 and 24 mM 3-nitrobenzoate, by both freely suspended Cells and Immobilized Cells, in batches and fed-batch with shaken cultures were compared. The PUF-Immobilized Cells achieved higher degradation rates of 12 and 24 mM — nitrobenzoate than freely suspended Cells, and the Cells Immobilized in SA-PVA, polyacrylamide, SA and agar. The PUF-Immobilized Cells could be reused for more than 21 cycles without losing any degradation capacity. These results revealed the feasibility of using PUF-Immobilized Cells of B. flexus for the enhanced degradation of — nitrobenzoate.
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Biodegradation of p-cresol by Immobilized Cells of Bacillus sp. strain PHN 1.
Biodegradation, 2008Co-Authors: Preeti N. Tallur, Veena B. Megadi, Harichandra Z. NinnekarAbstract:The Bacillus sp. strain PHN 1 capable of degrading p-cresol was Immobilized in various matrices namely, polyurethane foam (PUF), polyacrylamide, alginate and agar. The degradation rates of 20 and 40 mM p-cresol by the freely suspended Cells and Immobilized Cells in batches and semi-continuous with shaken cultures were compared. The PUF-Immobilized Cells achieved higher degradation of 20 and 40 mM p-cresol than freely suspended Cells and the Cells Immobilized in polyacrylamide, alginate and agar. The PUF- Immobilized Cells could be reused for more than 35 cycles, without losing any degradation capacity and showed more tolerance to pH and temperature changes than free Cells. These results revealed that the Immobilized cell systems are more efficient than freely suspended Cells for degradation of p-cresol.
