Immunoproteomics

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Annalisa Santucci - One of the best experts on this subject based on the ideXlab platform.

  • Helicobacter pylori Immunoproteomics in gastric cancer and gastritis of the carcinoma phenotype
    Expert review of proteomics, 2010
    Co-Authors: Edith Lahner, Giulia Bernardini, Annalisa Santucci, Bruno Annibale
    Abstract:

    Helicobacter pylori infection is linked to the development of gastric cancer. Atrophic body gastritis is considered the first important step in the histogenesis of such neoplasia. H. pylori infection is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because of the progressive disappearance of the bacterium. Host-pathogen interactions may be investigated by means of Immunoproteomics, which provides global information regarding the host humoral response to H. pylori infection and allows the identification of relevant specific and nonspecific antigens, and can be used for diagnostic or prognostic purposes. In the present review, we describe how several research groups used H. pylori Immunoproteomics to investigate highly immunoreactive bacterial antigens related to the development of gastric cancer.

  • Helicobacter pylori: Immunoproteomics related to different pathologies.
    Expert review of proteomics, 2007
    Co-Authors: Giulia Bernardini, Daniela Braconi, Paola Lusini, Annalisa Santucci
    Abstract:

    Helicobacter pylori is a Gram-negative bacterium that causes ulcer, atrophic gastritis, adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. Moreover, an ongoing controversial role of this bacterium infection has been suggested in the etiopathogenesis of some extradigestive diseases. The humoral response to H. pylori during a natural infection can be used for diagnostic purposes and as a basis for vaccine development. Host-pathogen interactions may be investigated by means of Immunoproteomics, which provides global information about relevant specific and nonspecific antigens, and thus might be suitable to identify novel vaccine candidates or serological markers of H. pylori infection as well as of different related diseases. In this review, we describe how several research groups used H. pylori proteomics combined with western blotting analysis, using sera from patients affected with different H. pylori-related pathologies, to investigate potential associations between host immune response and clinical outcomes of H. pylori infection, resulting in the rapid identification of novel, highly immunoreactive antigens.

  • Comparative proteomics and Immunoproteomics of Helicobacter pylori related to different gastric pathologies.
    Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2006
    Co-Authors: Roberta Mini, Giulia Bernardini, Anna Maria Salzano, Giovanni Renzone, Andrea Scaloni, Natale Figura, Annalisa Santucci
    Abstract:

    Helicobacter pylori is a Gram-negative bacterium which causes ulcer, atrophic gastritis, adenocarcinoma, or mucosa-associated lymphoid tissue lymphoma. A comparative proteomic and immunoproteomic analysis was chosen to identify the antigenic patterns of three different H. pylori strains. These strains were probed against single sera from H. pylori-positive patients affected by gastric adenocarcinoma or duodenal ulcer. We found a quite heterogeneous antigenic pattern, both from strain and sera points of view, thus underlying both a strain- and a host-specificity. The different antigenic repertoires introduced the importance of the strain to be used for immunoblotting as a diagnostic test.

Sanying Wang - One of the best experts on this subject based on the ideXlab platform.

  • identification of novel immunogenic proteins of shigella flexneri 2a by proteomic methodologies
    Vaccine, 2004
    Co-Authors: Xuanxian Peng, Sanying Wang
    Abstract:

    Shigella spp. are one of the most important etiological factors for people who are living in developing countries and travelers to tropical countries. High priority has been given by the World Health Organization to the development of vaccines to control Shigellosis caused by these bacteria. However, information regarding to profile of immunogenic proteins of Shigella is not available now. In the present study, sub-Immunoproteomics was applied to screen novel immunogenic proteins which could be reacted with antisera produced by challenge of a whole bacterium. Our results indicated that 13 immunogens were identified, in which seven proteins and six proteins from outer membrane and soluble proteome, respectively. Of the 13 proteins, 12 showed to be novel immunogens. These results suggest that Immunoproteomics can greatly improve the chances of identification and result in discovery of novel immunogenic proteins.

  • Rapid screening of highly efficient vaccine candidates by Immunoproteomics.
    Proteomics, 2004
    Co-Authors: Chen Zijun, Sanying Wang, Bo Peng, Xuanxian Peng
    Abstract:

    Diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. Therefore, it is very important to identify highly efficient immunogens for immune prevention. By combining Immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. Our approach may be divided into three consecutive steps. First, dominant immunogens of outer membrane proteins are screened by Immunoproteomics. Second, proteins with the ability to induce production of neutralizing antibodies are identified from the immunogens by virulent bacterium challenge following vaccination. Third, vaccine candidates are determined by evaluation of neutralizing abilities. Information on the candidates has been obtained for further gene cloning by mass spectrometry. Our results indicate that highly efficient protected antigens were identified from the outer membrane proteome of Aeromonas hydrophila, in which an immunogen showed 71.4% protective ability with multivalent functions to A. hydrophila and Aeromonas sobria. In summary, we have developed a high-throughout, accurate, rapid and highly efficient method which will play an active role in immune prevention for microbiological diseases.

Barbara M. Bröker - One of the best experts on this subject based on the ideXlab platform.

  • Elucidating the anti‐Staphylococcus aureus antibody response by Immunoproteomics
    Proteomics. Clinical applications, 2016
    Co-Authors: Sebastian Stentzel, Regine Gläser, Barbara M. Bröker
    Abstract:

    Staphylococcus aureus is a notorious pathogen both in- and outside hospitals as well as a frequent colonizer in healthy individuals. Immunoproteomics techniques have been employed to shed light on the human adaptive immune response to S. aureus in health and disease. Since priming of immune memory, a key property of the adaptive immune system, is the basis of successful vaccination, Immunoproteomics holds promise for paving the way to an effective S. aureus vaccine.

  • elucidating the anti staphylococcus aureus antibody response by Immunoproteomics
    Proteomics Clinical Applications, 2016
    Co-Authors: Sebastian Stentzel, Regine Gläser, Barbara M. Bröker
    Abstract:

    Staphylococcus aureus is a notorious pathogen both in- and outside hospitals as well as a frequent colonizer in healthy individuals. Immunoproteomics techniques have been employed to shed light on the human adaptive immune response to S. aureus in health and disease. Since priming of immune memory, a key property of the adaptive immune system, is the basis of successful vaccination, Immunoproteomics holds promise for paving the way to an effective S. aureus vaccine.

  • omics approaches for the study of adaptive immunity to staphylococcus aureus and the selection of vaccine candidates
    Proteome, 2016
    Co-Authors: Silva Holtfreter, Sebastian Stentzel, Julia Kolata, Stephanie S Bauerfeind, Frank Schmidt, Nandakumar Sundaramoorthy, Barbara M. Bröker
    Abstract:

    Staphylococcus aureus is a dangerous pathogen both in hospitals and in the community. Due to the crisis of antibiotic resistance, there is an urgent need for new strategies to combat S. aureus infections, such as vaccination. Increasing our knowledge about the mechanisms of protection will be key for the successful prevention or treatment of S. aureus invasion. Omics technologies generate a comprehensive picture of the physiological and pathophysiological processes within cells, tissues, organs, organisms and even populations. This review provides an overview of the contribution of genomics, transcriptomics, proteomics, metabolomics and Immunoproteomics to the current understanding of S. aureus‑host interaction, with a focus on the adaptive immune response to the microorganism. While antibody responses during colonization and infection have been analyzed in detail using Immunoproteomics, the full potential of omics technologies has not been tapped yet in terms of T-cells. Omics technologies promise to speed up vaccine development by enabling reverse vaccinology approaches. In consequence, omics technologies are powerful tools for deepening our understanding of the “superbug” S. aureus and for improving its control.

Cuifen Huang - One of the best experts on this subject based on the ideXlab platform.

  • Immunoproteomics of membrane proteins of shigella flexneri 2a 2457t
    World Journal of Gastroenterology, 2005
    Co-Authors: Tianyi Ying, Junjun Wang, Hengliang Wang, Erling Feng, Kaihua Wei, Liuyu Huang, Peitang Huang, Cuifen Huang
    Abstract:

    AIM: To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T. METHODS: The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) was performed to determine the molecular weight of peptides. Electrospray ionization (ESI-MS/MS) was performed to determine the sequences of the interesting peptides. RESULTS: A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein (YaeT) was detected, which might be a candidate target of vaccine. CONCLUSION: Membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum.

Xuanxian Peng - One of the best experts on this subject based on the ideXlab platform.

  • identification of novel immunogenic proteins of shigella flexneri 2a by proteomic methodologies
    Vaccine, 2004
    Co-Authors: Xuanxian Peng, Sanying Wang
    Abstract:

    Shigella spp. are one of the most important etiological factors for people who are living in developing countries and travelers to tropical countries. High priority has been given by the World Health Organization to the development of vaccines to control Shigellosis caused by these bacteria. However, information regarding to profile of immunogenic proteins of Shigella is not available now. In the present study, sub-Immunoproteomics was applied to screen novel immunogenic proteins which could be reacted with antisera produced by challenge of a whole bacterium. Our results indicated that 13 immunogens were identified, in which seven proteins and six proteins from outer membrane and soluble proteome, respectively. Of the 13 proteins, 12 showed to be novel immunogens. These results suggest that Immunoproteomics can greatly improve the chances of identification and result in discovery of novel immunogenic proteins.

  • Rapid screening of highly efficient vaccine candidates by Immunoproteomics.
    Proteomics, 2004
    Co-Authors: Chen Zijun, Sanying Wang, Bo Peng, Xuanxian Peng
    Abstract:

    Diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. Therefore, it is very important to identify highly efficient immunogens for immune prevention. By combining Immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. Our approach may be divided into three consecutive steps. First, dominant immunogens of outer membrane proteins are screened by Immunoproteomics. Second, proteins with the ability to induce production of neutralizing antibodies are identified from the immunogens by virulent bacterium challenge following vaccination. Third, vaccine candidates are determined by evaluation of neutralizing abilities. Information on the candidates has been obtained for further gene cloning by mass spectrometry. Our results indicate that highly efficient protected antigens were identified from the outer membrane proteome of Aeromonas hydrophila, in which an immunogen showed 71.4% protective ability with multivalent functions to A. hydrophila and Aeromonas sobria. In summary, we have developed a high-throughout, accurate, rapid and highly efficient method which will play an active role in immune prevention for microbiological diseases.