The Experts below are selected from a list of 288957 Experts worldwide ranked by ideXlab platform
Thomas L Toth - One of the best experts on this subject based on the ideXlab platform.
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in Vitro Culture of ovarian follicles from peromyscus
Seminars in Cell & Developmental Biology, 2017Co-Authors: Xiaoming He, Thomas L TothAbstract:The ovarian follicle is the fundamental functional tissue unit of mammalian ovary. Each ovarian follicle contains one single oocyte. Isolation and in Vitro Culture of ovarian follicles to obtain fertilizable oocytes have been regarded as a promising strategy for women to combat infertility. The follicles from Peromyscus are considered as a better model than that from inbred mice for studying follicle Culture. This is because Peromyscus mice are outbred (as with humans) with an increased life span. in this article, we reviewed studies on this subject conducted using Peromyscus follicles. These studies show that the conventional 2D micro-drop and 3D hanging-drop approaches established for in Vitro Culture of early preantral follicles from inbred mice are not directly applicable for cultivating the follicles from Peromyscus. However, the efficiency could be significantly improved by culturing multiple early preantral follicles in one hanging drop of Peromyscus ovarian cell-conditioned medium. It is further revealed that the mechanical heterogeneity in the extracellular matrix of ovary is crucial for developing early preantral follicles to the antral stage and for the subsequent ovulation to release cumulus-oocyte complex. These findings may provide valuable guidance for furthering the technology of in Vitro follicle Culture to restore fertility in the clinic.
Nasser Ghaderi - One of the best experts on this subject based on the ideXlab platform.
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Application of iron nanoparticles and salicylic acid in in Vitro Culture of strawberries (Fragaria × ananassa Duch.) to cope with drought stress
Plant Cell Tissue and Organ Culture (PCTOC), 2017Co-Authors: Ali Akbar Mozafari, Faride Havas, Nasser GhaderiAbstract:The effects of iron nanoparticles and salicylic acid (SA) on strawberry (Fragaria × ananassa Duch.) plants in conditions of drought stress were surveyed under in Vitro conditions to find the optimum combination for strawberry tissue Culture. Cuttings of the Queen Elisa cultivar were surveyed in a three-way factorial experiment with three replications in 2015. The results showed that drought stress significantly affected all measured parameters of strawberry plantlets under in Vitro condition in a negative way. SA compensated for the negative effects of drought stress on strawberry plantlets and improved their growth parameters under in Vitro Culture. Strawberry plantlets treated with iron nanoparticles were able to cope with stressful conditions better than untreated ones. This study found that iron, a micronutrient in plant growth and in Vitro development, greatly influenced the plantlets’ growth parameters and other measured traits. These results indicate that the efficiency of tissue Culture and in Vitro Culture of strawberries could be improved by increased application of iron in the form of nanoparticles. The results might also indicate that the application of iron nanoparticles along with SA can be a useful method for providing higher quantity and quantity in the in Vitro Culture of strawberries, and could be used for adapting strawberry plants to drought before transplanting them in the field.
David H. Adams - One of the best experts on this subject based on the ideXlab platform.
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Decreased porcine valve antigenicity with in Vitro Culture.
The Annals of Thoracic Surgery, 2001Co-Authors: Raymond H. Chen, David H. AdamsAbstract:Abstract Background . Porcine valvular prostheses may stimulate inflammation after implantation, with resultant accelerated structural degeneration. We investigated the expression of porcine major histocompatibility complex (MHC) class II molecules on valve leaflets and the possibility of decreasing valve antigenicity with in Vitro Culture. Methods . Aortic and pulmonary valves were harvested from domestic pigs under sterile conditions and Cultured in Vitro with either porcine or baboon serum for 4 days. Valves were harvested daily and fixed in Carnoy's or formalin solution. Microtome sections of valves were examined by hematoxylin and eosin, and by immunohistochemistry for porcine MHC class II proteins and an endothelial marker, α- N -acetylgalactosaminyl glycoprotein (α-GalNac). Results . Porcine aortic and pulmonary valves constitutively express α-GalNac proteins and porcine MHC class II antigens. Porcine valves continue to express both α-GalNac and MHC class II after 48 hours of Culture in porcine serum. After 48-hour Culture in baboon serum, however, MHC class II antigens became undetectable on valvular leaflets, although α-GalNac molecules were still detected. Conclusions . Porcine valvular endothelial cells remain viable after 2 days of in Vitro Culture. Porcine valves Cultured with primate serum show decreased MHC class II antigenic expression. in Vitro Culture before glutaraldehyde fixation may decrease inflammation associated with implantation.
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Decreased porcine valve antigenicity with in Vitro Culture.
The Annals of thoracic surgery, 2001Co-Authors: Raymond H. Chen, David H. AdamsAbstract:Porcine valvular prostheses may stimulate inflammation after implantation, with resultant accelerated structural degeneration. We investigated the expression of porcine major histocompatibility complex (MHC) class II molecules on valve leaflets and the possibility of decreasing valve antigenicity with in Vitro Culture. Aortic and pulmonary valves were harvested from domestic pigs under sterile conditions and Cultured in Vitro with either porcine or baboon serum for 4 days. Valves were harvested daily and fixed in Carnoy's or formalin solution. Microtome sections of valves were examined by hematoxylin and eosin, and by immunohistochemistry for porcine MHC class II proteins and an endothelial marker, alpha-N-acetylgalactosaminyl glycoprotein (alpha-GalNac). Porcine aortic and pulmonary valves constitutively express alpha-GalNac proteins and porcine MHC class II antigens. Porcine valves continue to express both alpha-GalNac and MHC class II after 48 hours of Culture in porcine serum. After 48-hour Culture in baboon serum, however, MHC class II antigens became undetectable on valvular leaflets, although alpha-GalNac molecules were still detected. Porcine valvular endothelial cells remain viable after 2 days of in Vitro Culture. Porcine valves Cultured with primate serum show decreased MHC class II antigenic expression. in Vitro Culture before glutaraldehyde fixation may decrease inflammation associated with implantation.
N Shanmugasundaram - One of the best experts on this subject based on the ideXlab platform.
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collagen chitosan polymeric scaffolds for the in Vitro Culture of human epidermoid carcinoma cells
Biomaterials, 2001Co-Authors: N Shanmugasundaram, P Ravichandran, Neelakanta P Reddy, Nalini Ramamurty, Subrata Pal, Panduranga K RaoAbstract:A biodegradable polymer scaffold was developed using collagen and chitosan, in the form of interpenetrating polymeric network (IPN), for in Vitro Culture of human epidermoid carcinoma cells (HEp-2, Cincinnati). Glutaraldehyde was used as cross-linking agent for the development of scaffold. Various types of scaffolds were prepared using different proportionate mixtures of collagen and chitosan solutions in the ratio of 3:7, 4:6, 5:5, 6:4 and 7:3 (collagen:chitosan). These scaffolds were fully characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Equilibrium swelling studies were carried out in phosphate buffer of physiological pH (7.4) to study its swelling characteristics at slightly alkaline pH. The scaffold that showed optimum swelling property was selected as the best scaffold for performing in Vitro Culture studies. in Vitro Culture studies were carried out using HEp-2 cells, over the selected scaffold and its growth morphology was determined through optical photographs taken at different magnifications at various days of Culture. The results of the above studies suggest that the scaffolds prepared from collagen and chitosan can be utilized as a substrate to Culture HEp-2 cells and can also be used as an in Vitro model to test anticancerous drugs.
Xiaoming He - One of the best experts on this subject based on the ideXlab platform.
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in Vitro Culture of ovarian follicles from peromyscus
Seminars in Cell & Developmental Biology, 2017Co-Authors: Xiaoming He, Thomas L TothAbstract:The ovarian follicle is the fundamental functional tissue unit of mammalian ovary. Each ovarian follicle contains one single oocyte. Isolation and in Vitro Culture of ovarian follicles to obtain fertilizable oocytes have been regarded as a promising strategy for women to combat infertility. The follicles from Peromyscus are considered as a better model than that from inbred mice for studying follicle Culture. This is because Peromyscus mice are outbred (as with humans) with an increased life span. in this article, we reviewed studies on this subject conducted using Peromyscus follicles. These studies show that the conventional 2D micro-drop and 3D hanging-drop approaches established for in Vitro Culture of early preantral follicles from inbred mice are not directly applicable for cultivating the follicles from Peromyscus. However, the efficiency could be significantly improved by culturing multiple early preantral follicles in one hanging drop of Peromyscus ovarian cell-conditioned medium. It is further revealed that the mechanical heterogeneity in the extracellular matrix of ovary is crucial for developing early preantral follicles to the antral stage and for the subsequent ovulation to release cumulus-oocyte complex. These findings may provide valuable guidance for furthering the technology of in Vitro follicle Culture to restore fertility in the clinic.
