The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Hopi E. Hoekstra - One of the best experts on this subject based on the ideXlab platform.
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Peromyscus mice as a model for studying natural variation
eLife, 2015Co-Authors: Nicole L Bedford, Hopi E. HoekstraAbstract:The deer mouse (genus Peromyscus) is the most abundant mammal in North America, and it occupies almost every type of terrestrial habitat. It is not surprising therefore that the natural history of Peromyscus is among the best studied of any small mammal. For decades, the deer mouse has contributed to our understanding of population genetics, disease ecology, longevity, endocrinology and behavior. Over a century's worth of detailed descriptive studies of Peromyscus in the wild, coupled with emerging genetic and genomic techniques, have now positioned these mice as model organisms for the study of natural variation and adaptation. Recent work, combining field observations and laboratory experiments, has lead to exciting advances in a number of fields—from evolution and genetics, to physiology and neurobiology.
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reproductive protein evolution within and between species maintenance of divergent zp3 alleles in Peromyscus
Molecular Ecology, 2008Co-Authors: Leslie M Turner, Hopi E. HoekstraAbstract:In a variety of animal taxa, proteins involved in reproduction evolve more rapidly than nonreproductive proteins. Most studies of reproductive protein evolution, however, focus on divergence between species, and little is known about differentiation among populations within a species. Here we investigate the molecular population genetics of the protein ZP3 within two Peromyscus species. ZP3 is an egg coat protein involved in primary binding of egg and sperm and is essential for fertilization. We find that amino acid polymorphism in the sperm-combining region of ZP3 is high relative to silent polymorphism in both species of Peromyscus. In addition, while there is geographical structure at a mitochondrial gene (Cytb), a nuclear gene (Lcat) and eight microsatellite loci, we find no evidence for geographical structure at Zp3 in Peromyscus truei. These patterns are consistent with the maintenance of ZP3 alleles by balancing selection, possibly due to sexual conflict or pathogen resistance. However, we do not find evidence that reinforcement promotes ZP3 diversification; allelic variation in P. truei is similar among populations, including populations allopatric and sympatric with sibling species. In fact, most alleles are present in all populations sampled across P. truei's range. While additional data are needed to identify the precise evolutionary forces responsible for sequence variation in ZP3, our results suggest that in Peromyscus, selection to maintain divergent alleles within species contributes to the pattern of rapid amino acid substitution observed among species.
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Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus
Molecular Ecology Notes, 2006Co-Authors: Lynne Marie Mullen, Rachel J. Hirschmann, Kelly L. Prince, Travis C. Glenn, Michael J. Dewey, Hopi E. HoekstraAbstract:We isolated and characterized 60 novel microsatellite markers from the closely related oldfield mouse ( Peromyscus polionotus ) and deer mouse ( Peromyscus maniculatus ) for studies of conservation, ecological, quantitative and population genetics. We assessed all 60 markers in a wild population of Peromyscus polionotus rhoadsi ( N = 20) from central Florida and found an average of nine alleles per marker and an observed heterozygosity ( H O ) of 0.66 (range = 0.00–1.00). These polymorphic markers contribute to the growing number of genomic resources for Peromyscus , an emerging model system for ecological and evolutionary research.
Jason Munshisouth - One of the best experts on this subject based on the ideXlab platform.
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urbanization shapes the demographic history of a native rodent the white footed mouse Peromyscus leucopus in new york city
Biology Letters, 2016Co-Authors: Stephen E Harris, Diego F Alvaradoserrano, J T Boehm, Tyler Joseph, Michael J Hickerson, Jason MunshisouthAbstract:How urbanization shapes population genomic diversity and evolution of urban wildlife is largely unexplored. We investigated the impact of urbanization on white-footed mice, Peromyscus leucopus, in ...
Michael J. Dewey - One of the best experts on this subject based on the ideXlab platform.
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Caring for Peromyscus spp. In research environments
Lab Animal, 2014Co-Authors: Janet P. Crossland, Michael R. Felder, Michael J. Dewey, Paul B Vrana, Shayne C. Barlow, Gabor SzalaiAbstract:Peromyscus spp. are the most abundant native North American mammals. They have gained popularity as research animals in the last 20 years, and this trend is expected to continue as new research tools, such as whole genome sequences, baseline physiological data and others, become available. Concurrently, advances have been made in the recommendations for the care of laboratory animals. The authors provide insight into how the Peromyscus Genetic Stock Center successfully breeds and maintains several stocks of deer mice and related species. This information is beneficial to researchers that plan to include Peromyscus spp. in their research programs.
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Peromyscus (deer mice) as developmental models
Wiley Interdisciplinary Reviews-Developmental Biology, 2013Co-Authors: Paul B Vrana, Monika Veres, Janet P. Crossland, Gabor Szalai, Michael R. Felder, Kimberly R Shorter, Jasmine E. Allen, Christopher D. Wiley, Amanda R. Duselis, Michael J. DeweyAbstract:Deer mice (Peromyscus) are the most common native North American mammals, and exhibit great natural genetic variation. Wild-derived stocks from a number of populations are available from the Peromyscus Genetic Stock Center (PGSC). The PGSC also houses a number of natural variants and mutants (many of which appear to differ from Mus). These include metabolic, coat-color/pattern, neurological, and other morphological variants/mutants. Nearly all these mutants are on a common genetic background, the Peromyscus maniculatus BW stock. Peromyscus are also superior behavior models in areas such as repetitive behavior and pair-bonding effects, as multiple species are monogamous. While Peromyscus development generally resembles that of Mus and Rattus, prenatal stages have not been as thoroughly studied, and there appear to be intriguing differences (e.g., longer time spent at the two-cell stage). Development is greatly perturbed in crosses between P. maniculatus (BW) and Peromyscus polionotus (PO). BW females crossed to PO males produce growth-restricted, but otherwise healthy, fertile offspring which allows for genetic analyses of the many traits that differ between these two species. PO females crossed to BW males produce overgrown but severely dysmorphic conceptuses that rarely survive to late gestation. There are likely many more uses for these animals as developmental models than we have described here. Peromyscus models can now be more fully exploited due to the emerging genetic (full linkage map), genomic (genomes of four stocks have been sequenced) and reproductive resources. For further resources related to this article, please visit the WIREs website. Conflict of interest: The authors have declared no conflicts of interest for this article.
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A unique late-replicating XY to autosome translocation in Peromyscus melanophrys
Chromosome Research, 2010Co-Authors: Elisabeth E. Mlynarski, Michael J. Dewey, Craig Obergfell, Rachel J. O’neillAbstract:We report on the characterization of the Peromyscus melanophrys karyotype and sex chromosome system. Classic studies reported the sex chromosome system of this species may be as complex as an X_1X_1X_2X_2/X_1X_2Y_1Y_2 and provided conflicting identification of the X chromosome. Using Peromyscus maniculatus chromosome paints, we have positively identified the sex chromosomes and clarified the sex determining system that once perplexed Peromyscus researchers. The sex chromosomes are characterized by a unique autosomal translocation of DNA shared between both the X and Y chromosomes. The translocated material is late replicating and heterochromatic yet retains the active chromatin conformation. Thus, autosomal regions derived from translocations involving repeat-rich material may retain some epigenetic marks specific to the sex chromosomes despite loss of epigenetic silencing activity.
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Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus
Molecular Ecology Notes, 2006Co-Authors: Lynne Marie Mullen, Rachel J. Hirschmann, Kelly L. Prince, Travis C. Glenn, Michael J. Dewey, Hopi E. HoekstraAbstract:We isolated and characterized 60 novel microsatellite markers from the closely related oldfield mouse ( Peromyscus polionotus ) and deer mouse ( Peromyscus maniculatus ) for studies of conservation, ecological, quantitative and population genetics. We assessed all 60 markers in a wild population of Peromyscus polionotus rhoadsi ( N = 20) from central Florida and found an average of nine alleles per marker and an observed heterozygosity ( H O ) of 0.66 (range = 0.00–1.00). These polymorphic markers contribute to the growing number of genomic resources for Peromyscus , an emerging model system for ecological and evolutionary research.
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cross species amplification among peromyscines of new microsatellite dna loci from the oldfield mouse Peromyscus polionotus subgriseus
Molecular Ecology Notes, 2002Co-Authors: Kelly L. Prince, Travis C. Glenn, Michael J. DeweyAbstract:We describe polymerase chain reaction (PCR) primers and conditions to amplify 11 microsatellite DNA loci isolated from the oldfield mouse (Peromyscus polionotus subgriseus). These were tested for amplification using nine species and subspecies maintained at the Peromyscus Genetic Stock Center, with an average success rate of 65% and two loci amplifying in all species. Polymorphism was tested within the P. polionotus subgriseus and the recently obtained P. maniculatus sonorensis colonies. P. p. subgriseus had modest numbers of alleles per locus (1–4), whereas P. m. sonorensis had many alleles per locus (5–10) and high expected heterozygosities (0.625–0.878).
Matthew D Macmanes - One of the best experts on this subject based on the ideXlab platform.
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characterization of a male reproductive transcriptome for Peromyscus eremicus cactus mouse
PeerJ, 2016Co-Authors: Lauren Kordonowy, Matthew D MacmanesAbstract:Rodents of the genus Peromyscus have become increasingly utilized models for investigations into adaptive biology. This genus is particularly powerful for research linking genetics with adaptive physiology or behaviors, and recent research has capitalized on the unique opportunities afforded by the ecological diversity of these rodents. Well characterized genomic and transcriptomic data is intrinsic to explorations of the genetic architecture responsible for ecological adaptations. Therefore, this study characterizes the transcriptome of three male reproductive tissues (testes, epididymis and vas deferens) of Peromyscus eremicus (Cactus mouse), a desert specialist. The transcriptome assembly process was optimized in order to produce a high quality and substantially complete annotated transcriptome. This composite transcriptome was generated to characterize the expressed transcripts in the male reproductive tract of P. eremicus, which will serve as a crucial resource for future research investigating our hypothesis that the male Cactus mouse possesses an adaptive reproductive phenotype to mitigate water-loss from ejaculate. This study reports genes under positive selection in the male Cactus mouse reproductive transcriptome relative to transcriptomes from Peromyscus maniculatus (deer mouse) and Mus musculus. Thus, this study expands upon existing genetic research in this species, and we provide a high quality transcriptome to enable further explorations of our proposed hypothesis for male Cactus mouse reproductive adaptations to minimize seminal fluid loss.
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characterization of a male reproductive transcriptome for Peromyscus eremicus cactus mouse
bioRxiv, 2016Co-Authors: Lauren Kordonowy, Matthew D MacmanesAbstract:Rodents of the genus Peromyscus have become increasingly utilized models for investigations into adaptive biology. This genus is particularly powerful for research linking genetics with adaptive physiology and behaviors, and recent research has capitalized on the unique opportunities afforded by the ecological diversity of these rodents. However, well characterized genomic and transcriptomic data is intrinsic to explorations of the genetic architecture responsible for ecological adaptations. This study characterizes a reproductive transcriptome of male Peromyscus eremicus (Cactus mouse), a desert specialist with extreme physiological adaptations to water limitation. We describe a reproductive transcriptome comprising three tissues in order to expand upon existing research in this species and to facilitate further studies elucidating the genetic basis of potential desert adaptations in male reproductive physiology.
Janet P. Crossland - One of the best experts on this subject based on the ideXlab platform.
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Growth of human breast cancers in Peromyscus
Disease Models & Mechanisms, 2018Co-Authors: Vimala Kaza, Janet P. Crossland, Amanda Havighorst, Elena Farmaki, Ioulia Chatzistamou, Hippokratis KiarisAbstract:ABSTRACT Modeling breast cancer in general and hormone-sensitive breast cancer, in particular in mice, has several limitations. These are related to the inbred nature of laboratory mice, and do not allow adequate appreciation of the contribution of the host9s genetic heterogeneity in tumor growth. In addition, the naturally low estrogen levels of mice makes estradiol supplementation obligatory for tumor growth. Here, we show that Peromyscus californicus , following cyclosporine-mediated immunosuppression, supports the growth of both MDA-MB-231 estrogen-independent and MCF7 estrogen receptor-positive breast cancers without exogenous estradiol supplementation. Tumor growth was inhibited by fulvestrant or letrozole, confirming that MCF7 xenografts remain hormone dependent in vivo and suggesting that P. californicus can be used as an alternative to conventional mice for the study of hormone-sensitive breast cancer. The fact that Peromyscus stocks are outbred also facilitates the study of breast cancer in genetically heterogenous populations.
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a method to distinguish morphologically similar Peromyscus species using extracellular rna and high resolution melt analysis
Analytical Biochemistry, 2016Co-Authors: Veronica A Seifert, Janet P. Crossland, Benjamin L Clarke, Lynne T BemisAbstract:A method applying high-resolution melt (HRM) analysis to PCR products copied and amplified from extracellular RNA (exRNA) has been developed to distinguish two morphologically similar Peromyscus species: Peromyscus leucopus and Peromyscus maniculatus. P. leucopus is considered the primary reservoir host of Borrelia burgdorferi, the causative agent for Lyme disease in North America. In northern Minnesota the habitat ranges of P. leucopus overlaps with that of P. maniculatus. Serum samples from live mice of both species were collected from cheek bleeds, total extracellular RNA (exRNA) was extracted, copied using reverse transcription and amplified by PCR followed by HRM analysis. A circulating ribosomal RNA (rRNA) was identified which differed at seven nucleotides between the two species and a method of HRM analysis was developed allowing rapid species confirmation. In the future, this HRM based method may be adapted for additional species.
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Peromyscus as a model of human disease
Seminars in Cell & Developmental Biology, 2016Co-Authors: Amanda Havighorst, Janet P. Crossland, Hippokratis KiarisAbstract:Animals of the genus Peromyscus have been a particularly informative model for many areas of study, including behavior, evolution, anatomy, physiology and genetics. While their use in modeling human disease and pathology has been relatively restricted, certain qualities of Peromyscine mice may make them a good candidate for such studies. Pathophysiological conditions where Peromyscus may be of particular value involve aging, reactive oxygen species-associated pathologies, metabolism and detoxification, diabetes, and certain cancers. In this review article we will summarize pathological conditions where Peromyscus have been used effectively, we will discuss factors limiting the use of Peromyscus in studying pathology and we will indicate areas at which the use of this model may be of special value.
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Caring for Peromyscus spp. In research environments
Lab Animal, 2014Co-Authors: Janet P. Crossland, Michael R. Felder, Michael J. Dewey, Paul B Vrana, Shayne C. Barlow, Gabor SzalaiAbstract:Peromyscus spp. are the most abundant native North American mammals. They have gained popularity as research animals in the last 20 years, and this trend is expected to continue as new research tools, such as whole genome sequences, baseline physiological data and others, become available. Concurrently, advances have been made in the recommendations for the care of laboratory animals. The authors provide insight into how the Peromyscus Genetic Stock Center successfully breeds and maintains several stocks of deer mice and related species. This information is beneficial to researchers that plan to include Peromyscus spp. in their research programs.
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Peromyscus (deer mice) as developmental models
Wiley Interdisciplinary Reviews-Developmental Biology, 2013Co-Authors: Paul B Vrana, Monika Veres, Janet P. Crossland, Gabor Szalai, Michael R. Felder, Kimberly R Shorter, Jasmine E. Allen, Christopher D. Wiley, Amanda R. Duselis, Michael J. DeweyAbstract:Deer mice (Peromyscus) are the most common native North American mammals, and exhibit great natural genetic variation. Wild-derived stocks from a number of populations are available from the Peromyscus Genetic Stock Center (PGSC). The PGSC also houses a number of natural variants and mutants (many of which appear to differ from Mus). These include metabolic, coat-color/pattern, neurological, and other morphological variants/mutants. Nearly all these mutants are on a common genetic background, the Peromyscus maniculatus BW stock. Peromyscus are also superior behavior models in areas such as repetitive behavior and pair-bonding effects, as multiple species are monogamous. While Peromyscus development generally resembles that of Mus and Rattus, prenatal stages have not been as thoroughly studied, and there appear to be intriguing differences (e.g., longer time spent at the two-cell stage). Development is greatly perturbed in crosses between P. maniculatus (BW) and Peromyscus polionotus (PO). BW females crossed to PO males produce growth-restricted, but otherwise healthy, fertile offspring which allows for genetic analyses of the many traits that differ between these two species. PO females crossed to BW males produce overgrown but severely dysmorphic conceptuses that rarely survive to late gestation. There are likely many more uses for these animals as developmental models than we have described here. Peromyscus models can now be more fully exploited due to the emerging genetic (full linkage map), genomic (genomes of four stocks have been sequenced) and reproductive resources. For further resources related to this article, please visit the WIREs website. Conflict of interest: The authors have declared no conflicts of interest for this article.
