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Adrian Steiner - One of the best experts on this subject based on the ideXlab platform.
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Effects of a single administration of prostaglandin F2alpha, or a combination of prostaglandin F2alpha and prostaglandin E2, or placebo on fertility variables in dairy cows 3-5 weeks post partum, a randomized, double-blind clinical trial
Reproductive Biology and Endocrinology, 2006Co-Authors: Gaby Hirsbrunner, Heinz W. Burkhardt, Adrian SteinerAbstract:Delayed uterine involution has negative effects on the fertility of cows; use of prostaglandin F2alpha alone as a single treatment has not been shown to consistently improve fertility. Combined administration of PGF2alpha and PGE2 increased uterine pressure in healthy cows. We hypothesized, that the combination of both prostaglandins would accelerate uterine involution and have, therefore, a positive effect on fertility variables. In commercial dairy farming, the benefit of a single post partum combined prostaglandin treatment should be demonstrated. 383 cows from commercial dairy farms were included in this study. Uterine size and secretion were evaluated at treatment 21–35 days post partum and 14 days later. Cows were randomly allocated to one of three treatment groups: PGF2alpha and PGE2, PGF2alpha or placebo. For every animal participating in the study, the following reproduction variables were recorded: Interval from calving to first Insemination, days open, number of artificial Inseminations (AI) to conception; subsequent treatment of uterus, subsequent treatment of ovaries. Plasma progesterone level at time of treatment was used as a covariable. For continuous measurements, analysis of variance was performed. Fisher's exact test for categorical non-ordered data and exact Kruskal-Wallis test for ordered data were used; pairwise group comparisons with Bonferroni adjustment of significance level were performed. There was no significant difference among treatment groups in uterine size. Furthermore, there was no significant difference among treatments concerning days open, number of AI, and subsequent treatment of uterus and ovaries. Days from calving to first Insemination tended to be shorter for cows with low progesterone level given PGF2alpha and PGE2 in combination than for the placebo-group (P = 0.024). The results of this study indicate that the administration of PGF2alpha or a combination of PGF2alpha and PGE2 21 to 35 days post partum had no beneficial effect upon measured fertility variables. The exception was a tendency for a shorter interval from calving to first Insemination after administration of the combination of PGF2alpha and PGE2, as compared to the placebo group. Further research should be done in herds with reduced fertility and/or an increased incidence of postpartum vaginal discharge.
Gaby Hirsbrunner - One of the best experts on this subject based on the ideXlab platform.
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Effects of a single administration of prostaglandin F2alpha, or a combination of prostaglandin F2alpha and prostaglandin E2, or placebo on fertility variables in dairy cows 3-5 weeks post partum, a randomized, double-blind clinical trial
Reproductive Biology and Endocrinology, 2006Co-Authors: Gaby Hirsbrunner, Heinz W. Burkhardt, Adrian SteinerAbstract:Delayed uterine involution has negative effects on the fertility of cows; use of prostaglandin F2alpha alone as a single treatment has not been shown to consistently improve fertility. Combined administration of PGF2alpha and PGE2 increased uterine pressure in healthy cows. We hypothesized, that the combination of both prostaglandins would accelerate uterine involution and have, therefore, a positive effect on fertility variables. In commercial dairy farming, the benefit of a single post partum combined prostaglandin treatment should be demonstrated. 383 cows from commercial dairy farms were included in this study. Uterine size and secretion were evaluated at treatment 21–35 days post partum and 14 days later. Cows were randomly allocated to one of three treatment groups: PGF2alpha and PGE2, PGF2alpha or placebo. For every animal participating in the study, the following reproduction variables were recorded: Interval from calving to first Insemination, days open, number of artificial Inseminations (AI) to conception; subsequent treatment of uterus, subsequent treatment of ovaries. Plasma progesterone level at time of treatment was used as a covariable. For continuous measurements, analysis of variance was performed. Fisher's exact test for categorical non-ordered data and exact Kruskal-Wallis test for ordered data were used; pairwise group comparisons with Bonferroni adjustment of significance level were performed. There was no significant difference among treatment groups in uterine size. Furthermore, there was no significant difference among treatments concerning days open, number of AI, and subsequent treatment of uterus and ovaries. Days from calving to first Insemination tended to be shorter for cows with low progesterone level given PGF2alpha and PGE2 in combination than for the placebo-group (P = 0.024). The results of this study indicate that the administration of PGF2alpha or a combination of PGF2alpha and PGE2 21 to 35 days post partum had no beneficial effect upon measured fertility variables. The exception was a tendency for a shorter interval from calving to first Insemination after administration of the combination of PGF2alpha and PGE2, as compared to the placebo group. Further research should be done in herds with reduced fertility and/or an increased incidence of postpartum vaginal discharge.
H. Paulenz - One of the best experts on this subject based on the ideXlab platform.
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Effect of cervical and vaginal Insemination with liquid semen stored at room temperature on fertility of goats.
Animal reproduction science, 2020Co-Authors: H. Paulenz, Lennart Söderquist, Tormod Ådnøy, K Soltun, P A Saether, K R Fjellsøy, K Andersen BergAbstract:The effect of vaginal and cervical deposition of liquid semen stored at room temperature on the fertility of goats was tested in a field trial in which 217 Norwegian Dairy goats aged between 6 months and 7.5 years from 14 farms were inseminated after natural oestrous. Cervical Insemination with 200 x 10(6) spermatozoa resulted in 25-day non-return and kidding rates of 87.0 and 78.0%, and vaginal Insemination gave 85.5 and 74.3%, respectively. There was no significant difference between the cervical and vaginal Inseminations (P = 0.59 for the 25-day non-return and P = 0.40 for the kidding rates). Farm had a significant effect on the 25-day non-return rate (P = 0.03) but not on the kidding rate (P = 0.07). There were no significant differences between the fertility rates for different bucks (P = 0.36 for the 25-day non-return and P = 0.15 for the kidding rates). Fertility results after vaginal Insemination were encouragingly high. Vaginal Insemination is a simple, less costly and time consuming technique compared to others, also bringing into focus the animal welfare aspects of the artificial Insemination procedure. As the final goal is to establish a technique that could be applied similarly on a large scale by all farmers, vaginal Insemination must be considered as a method that would simplify the use of liquid buck semen in Norway.
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Fertility results after vaginal deposition of frozen-thawed buck semen diluted with two different extenders using one- or two-step procedures.
Reproduction in Domestic Animals, 2011Co-Authors: Nordstoga, Lennart Söderquist, Tormod Ådnøy, H. PaulenzAbstract:Contents In two field trials (T1 and T2), the effect of two different extenders for buck semen was tested. Semen from six (T1) and seven (T2) bucks of the Norwegian Dairy Goat breed was diluted either in a milk-based extender containing egg yolk (M) or in a commercially available extender without egg yolk [Andromed® (A)]. Dilution in M was performed in a two-step procedure including centrifugation of the ejaculates and removal of the supernatant, while dilution in A was performed in one step. During the two trials (T1 and T2) 514 and 714, does, respectively, were artificially inseminated during natural oestrus, and the farmers performed the Inseminations themselves after attending an artificial Insemination (AI) training course. Vaginal Insemination with 200 × 106 spermatozoa diluted in M resulted in a 25-day non-return rate (NRR) and kidding rate of 37.3% and 24.5%, respectively, while semen diluted in A resulted in 31.7% NRR and a kidding rate of 19.8% (T1). In T2, NRR and kidding rate for AI performed with semen diluted with M were 42.7% and 28.5%, respectively, while dilution in A gave 37.2% NRR and a kidding rate of 26.8%. There was no significant effect of extender in the two trials [T1:p = 0.068 (NRR), p = 0.148 (kidding rate), T2:p = 0.096 (NRR), p = 0.38 (kidding rate)], but farmer had a significant effect on the fertility parameters in both trials. In conclusion, the present studies may indicate that Andromed® is suitable for buck semen. However, more research is necessary to confirm the results and to improve the fertility of does after vaginal AI with frozen-thawed semen.
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Vaginal deposition of frozen-thawed semen in Norwegian Dairy goats: comparison of single and double Insemination with equal total number of spermatozoa.
Theriogenology, 2010Co-Authors: Nordstoga, Lennart Söderquist, Tormod Ådnøy, W. Farstad, H. PaulenzAbstract:Abstract In a field trial, a total of 472 Norwegian Dairy goats showing natural estrus were artificially inseminated with frozen-thawed semen. The farmers themselves performed vaginal deposition of 400 × 106 spermatozoa; one half of the does received two straws (200 × 106 spermatozoa/straw) at the same time (single AI), while the other half received two straws (200 × 106 spermatozoa/straw) 12 h apart (double AI). The commercially available extender Andromed® was used for dilution. The does were housed at 15 different farms, and on average 31 does were inseminated per farm. Non return rates (NRR) and kidding rates after single Insemination were 64.3% and 58.3%, respectively. Double Inseminations resulted in a NRR of 62% and a kidding rate of 57%. No significant difference between single and double AI was seen in the study. This study indicates that single or double vaginal Insemination with an equal total number of frozen-thawed spermatozoa (400 × 106) can give acceptable fertility results in Norwegian Dairy goats. However, studies on reducing sperm numbers are called for to allow AI donor bucks to be used to their fullest potential.
Heinz W. Burkhardt - One of the best experts on this subject based on the ideXlab platform.
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Effects of a single administration of prostaglandin F2alpha, or a combination of prostaglandin F2alpha and prostaglandin E2, or placebo on fertility variables in dairy cows 3-5 weeks post partum, a randomized, double-blind clinical trial
Reproductive Biology and Endocrinology, 2006Co-Authors: Gaby Hirsbrunner, Heinz W. Burkhardt, Adrian SteinerAbstract:Delayed uterine involution has negative effects on the fertility of cows; use of prostaglandin F2alpha alone as a single treatment has not been shown to consistently improve fertility. Combined administration of PGF2alpha and PGE2 increased uterine pressure in healthy cows. We hypothesized, that the combination of both prostaglandins would accelerate uterine involution and have, therefore, a positive effect on fertility variables. In commercial dairy farming, the benefit of a single post partum combined prostaglandin treatment should be demonstrated. 383 cows from commercial dairy farms were included in this study. Uterine size and secretion were evaluated at treatment 21–35 days post partum and 14 days later. Cows were randomly allocated to one of three treatment groups: PGF2alpha and PGE2, PGF2alpha or placebo. For every animal participating in the study, the following reproduction variables were recorded: Interval from calving to first Insemination, days open, number of artificial Inseminations (AI) to conception; subsequent treatment of uterus, subsequent treatment of ovaries. Plasma progesterone level at time of treatment was used as a covariable. For continuous measurements, analysis of variance was performed. Fisher's exact test for categorical non-ordered data and exact Kruskal-Wallis test for ordered data were used; pairwise group comparisons with Bonferroni adjustment of significance level were performed. There was no significant difference among treatment groups in uterine size. Furthermore, there was no significant difference among treatments concerning days open, number of AI, and subsequent treatment of uterus and ovaries. Days from calving to first Insemination tended to be shorter for cows with low progesterone level given PGF2alpha and PGE2 in combination than for the placebo-group (P = 0.024). The results of this study indicate that the administration of PGF2alpha or a combination of PGF2alpha and PGE2 21 to 35 days post partum had no beneficial effect upon measured fertility variables. The exception was a tendency for a shorter interval from calving to first Insemination after administration of the combination of PGF2alpha and PGE2, as compared to the placebo group. Further research should be done in herds with reduced fertility and/or an increased incidence of postpartum vaginal discharge.
Michéle Theau-Clément - One of the best experts on this subject based on the ideXlab platform.
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Influence of different eCG doses on sexual receptivity and productivity of rabbit does
World Rabbit Science, 2008Co-Authors: Michéle Theau-Clément, Fran??ois Lebas, Gabriele Brecchia, Cristiano Boiti, Philippe MercierAbstract:The aim of this study was to examine sexual receptivity and productivity of does injected 48 h before Insemination with 8 or 25 IU of eCG (groups 8 and 25, respectively) in comparison with a control group (no injection, group 0). One hundred twenty four does were inseminated during 11 consecutive cycles (reproduction rythm: 35 d; Inseminations: 4 d postpartum and weaning: 28 d postpartum). In comparison with the control group, eCG significantly improves the receptivity of does (60.3 vs 80.5 and 79.8%; P
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Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does
Animal, 2008Co-Authors: Michéle Theau-Clément, Fran??ois Lebas, Jean-françois Beckers, P V DrionAbstract:The aim of this experiment was to study the kinetics of anti-eCG (equine chorionic gonadotrophin) antibodies in relation to eCG dose (8 or 25 IU) and number of injections (h = 11) in comparison with a control group (no injection), and to relate antibody production to sexual receptivity and productivity of rabbit does. In all, 124 lactating primiparous rabbit does were inseminated every 35 days for a year Just before eCG injection (48 h before Insemination), blood samples were collected from all the does to assay anti-eCG antibodies. The anti-eCG antibody binding rate, regardless of the injected dose shows that none of the does developed detectable anti-eCG antibodies before the 7th injection. The level of detectable anti-eCG antibodies began to show an increase at the 7th injection and was significant only for the 25 IU dose at the 11 th injection. At the end of the experiment, 15% and 39% of does treated with 8 and 25 IU, respectively, developed immunity to eCG (binding rate >6%: higher binding rate of the control group). Consequently, the immune response depends on the eCG dose and on the number of injections. Moreover productivity of does estimated from the number of weaned rabbits produced per Insemination is not influenced by the level of eCG antibodies (7.0 and 6.9 for binding rate = 6%, respectively). Only 19 Inseminations (h = 6 and n = 13 for 8 and 25 IU, respectively) were made on hyperimmune does. Consequently, the immune response to eCG seems to be marginal for rabbit does. Moreover, under the described experimental conditions, reproductive performances of hyperimmune does were not affected.
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The relationship between rabbit semen characteristics and reproductive performance after artificial Insemination
Animal Reproduction Science, 2002Co-Authors: J M Brun, Michéle Theau-Clément, Gérard BoletAbstract:The relationships between several rabbit buck semen traits, concerning either the ejaculate or the dose inseminated (volume, mass motility, pH, percentage of motile sperm (PMS), concentration, number of total and motile sperm per ejaculate and per Insemination dose) and the reproductive performance of does was investigated in 839 Inseminations involving 54 bucks and 111 does. Four genetic types were involved: INRA1601 strain (A), INRA2066 strain (B) and the two reciprocal crossbreds (AB and BA). The mating design was A × A, B × B, (AB or BA) × (AB or BA). Semen was diluted (1:9) and a constant volume of 0.5 ml was inseminated 2-4h after collection. Therefore, the total number of spermatozoa per dose was variable and proportional to the initial concentration. Mass motility significantly influenced the kindling rate. Taken separately, volume, PMS and concentration did not influence the kindling rate but their product, the number of motile sperm per ejaculate, did. Litter size (total born) was significantly influenced by concentration and all variables depending on it, particularly the number of total and motile sperm per dose. However, reproductive performances were predominantly influenced by the physiological status of the does at Insemination (lactation stage and receptivity). © 2002 Elsevier Science B.V. All rights reserved.
