Intestine Flora

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Shen Tong - One of the best experts on this subject based on the ideXlab platform.

Wellington Monteiro Machado - One of the best experts on this subject based on the ideXlab platform.

Xu Wei-ling - One of the best experts on this subject based on the ideXlab platform.

Oliver Stuke - One of the best experts on this subject based on the ideXlab platform.

  • Untersuchungen zu möglichen infektions-prophylaktischen Effekten verschiedener Futterzusätze (Ameisen- und Zitronensäure, Lactulose, Pankreasenzyme) unter den Bedingungen einer experimentellen oralen Belastung mit E. coli (Absetzferkel) bzw. mit S. D
    2003
    Co-Authors: Oliver Stuke
    Abstract:

    The demand of alternatives to antibiotic therapeutics and performance promoting substances in swine production has in cause of legal restriction and high economic losses specific in the weaning of piglets come to interest. Also the risks to health of humans by spread out of salmonella from slaughter pigs is a serious danger. That`s why the search for non-antibiotic, performance-promoting substances in the area of organic acids, pre- and probiotics and enzymes has been started to stabilize the unstable balance of the intestinal Flora in the weaning period of piglets and also stop the adherance and increase of pathogene microorganism. Also the translocation and excretion of Salmonella from slaughter pigs should be stopped. In the setting of this thesis should be examined if the addition of coated acids, lactulose and coated pancreatic enzymes has an influence on the quality of excrements and chyme, the metabolic products of the Intestine Flora of weaned piglets and also if the occurence of the oedema disease under the terms of experimental infection by E. coli could be reduced in its frequence and intensity. Further the influence of lactulose on the quality of excrements and chymus and the metabolic products of the intesinal Flora of slaughter pigs with experimental infection by S. derby should be examinated. The own examinations have been carried out with 124 pigs, in an separate attempt with and without experimental infection of weaned piglets (n = 104) by E. coli for each feed-additive. The fattening pigs (n = 20)were experimental infected by S. derby. The pigs were feed a diet (15.2 – 16.2 MJ ME/kg TS, 192 – 207 g Rp/kg TS, 29.2 – 32.2 g Rfe/kg TS, 33.0 – 38.4 g Rfa/kg TS) ad libitum. In the examination with coated acids the food was pellet-formed given with 0,5% of the acid-product (ca. 45% formic acid and 21% citric acid), in the examination with lactulose and weaned piglets and also the fattening pigs the grind food  was given with 50 g lactulosesirup/kg (25.1 g lactulose), the controll pigs were given 5 % corn starch. The addition of coated pancreatic enzymes was 2.4 % soybean-pelett (96000 IE Lipase, 960000 IE Amylase, 60000 IE Protease) in grind feed, the control pigs was given 2.4 % . In the examinatinon with experimental  infection of E. coli and addition of coated pancreatic enzymes the controll piglets were given 5 mg Colistin /kg KM in the second passage. The piglets were weaned by 21 – 28 days and were feed awith the foodadditive after a short adaption and were sacrified after 4 – 16 days. In the examination with experimental infection by E. coli the animals were sacrified immediately by clinical signs of oedeme disease or after 9 – 16 days. The fattening pigs in the examination with S. derby were fed with the additive and experimental infected by S. derby after they were put in the barns, after 14 days p. i. Four pigs in each week were sacrified, the complete duration was 42 days. During the feeding phase the feed intake, daily increase and feed-expenditure, DM and pH in faeces and formiat-, lactulose- and amylase-concentration in the diet. In the section the stomach and intestinal contents was specified and the chymus quality characterized by DM and pH. By characterizing the metabolism products of the intestinal Flora (l-laktat, vfa, NH3 and LPS) possible effects of the feed additives was checked. In the examination with pancreatic enzymes also stearch and protein was specified in the stomach and the colon asc. Additional to the own examinations the intestinal Flora was characterized by direct microbiological methods in the thesis of BOLLMANN (2002). The results of the separate examinations can be represented as shown below: The dissolving of the coating of the acids has happened in the stomach and caused a release of the acids. An effect was not seen in the whole intestinal tract and also on the quality of faeces and metabolic products of the intestinal Flora. Also in the examination with experimental infection by E. coli there was no effect seen to the oedema disease of weaned piglets (from 20 infected piglets 6 were ill by the oedema disease, 3 in the controll group, 3 in the examination group). Lactulose was metabolized before the end of the small Intestine. The Lactulose had no influence on the quality of faeces and chymus and also not on the metabolic products of the intestinal Flora of weaned piglets and slaughter pigs. A trend to reduce the E. coli and ammonia-concentration could be seen at weaned piglets but the Lactulose had no influence on the oedeme disease (from 20 infected piglets 7 were ill by the oedema disease, 3 in the controll group, 4 in the examination group). The excretion and translocation of S. Derby at fattening pigs was not influenced. A dissolving of the coated pancreas enzymes has happened in the stomach, an influence of the enzymes on the distal Intestine could not be seen. In the examination without experimental infection and addition with coated pancres enzymes there were lower E. coli, but the enzymes had no influence on the oedeme disease (all of the 10 infected piglets were ill of the oedema disease). The feeding of Colistin (5 g/kg body weight) showed the effect of this antibiotic (none of the Colistin-given piglets were ill, the other piglets were all ill of the oedema disease), so the examination-model could be confirmed. The postulated effects of the food aditives could not be confirmed in this work. Also in the parallel to this thesis made microbiological examinations of BOLLMANN (2002) support this results, because in that thesis could not be seen essential effects of the food additives on the intestinal Flora.

Wu Xiao-ting - One of the best experts on this subject based on the ideXlab platform.

  • The applications of Intestine microecologic agents in postoperative patients with colorectal diseases
    Parenteral & Enteral Nutrition, 2007
    Co-Authors: Wu Xiao-ting
    Abstract:

    Objective: The study was designed to investigate the effect of Intestine microecologic agents in postoperative patients with colorectal diseases. Methods: 32 patients undergoing the colorectal operation were randomized into the study group and the control group. Both groups received standard enteral nutrition. The study group received the Intestine microecologic agents (golden bifido) from the 3rd day after the operation, but the control group didn't receive golden bifido. The parameters including gastrointestinal symptom, diarrhea and diarrhea score, intestinal Flora ratio were measured before and after the operation and the application of the agents. Results: ①Compared with the control group, the study group had less flatulence and abdominal pain(P0.05). ② The study group had less occurrences of diarrhea on the 6th day after the operation(P0.05). In the study group, the total occurrences and days of diarrhea were lower than those in the control group(P0.05). ③Intestinal Flora ratios were higher in the study group. Conclusion: The application of Intestine microecologic agents in postoperative patients with colorectal diseases is beneficial in alleviating gastrointestinal symptom and correcting Intestine Flora disturbance.