The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform
Hong Ji - One of the best experts on this subject based on the ideXlab platform.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of Chromatography B, 2010Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Abstract Puerarin is a major active ingredient of Pueraria Radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID–MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1 × 100 mm, 1.8 μm) at 30 °C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min −1 , and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min −1 , dry temperature: 350 °C, nebulizer: 40 psi, capillary: −3500 V, scan range: 250–800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7- O -glucuronide and puerarin-4′- O -glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10–15 and 15–30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Puerarin is a major active ingredient of Pueraria radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID-MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1x100 mm, 1.8 microm) at 30 degrees C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min(-1), and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min(-1), dry temperature: 350 degrees C, nebulizer: 40 psi, capillary: -3500 V, scan range: 250-800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7-O-glucuronide and puerarin-4'-O-glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10-15 and 15-30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
Mu Yuan - One of the best experts on this subject based on the ideXlab platform.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of Chromatography B, 2010Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Abstract Puerarin is a major active ingredient of Pueraria Radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID–MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1 × 100 mm, 1.8 μm) at 30 °C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min −1 , and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min −1 , dry temperature: 350 °C, nebulizer: 40 psi, capillary: −3500 V, scan range: 250–800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7- O -glucuronide and puerarin-4′- O -glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10–15 and 15–30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Puerarin is a major active ingredient of Pueraria radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID-MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1x100 mm, 1.8 microm) at 30 degrees C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min(-1), and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min(-1), dry temperature: 350 degrees C, nebulizer: 40 psi, capillary: -3500 V, scan range: 250-800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7-O-glucuronide and puerarin-4'-O-glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10-15 and 15-30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
Andreas Prescher - One of the best experts on this subject based on the ideXlab platform.
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Vascular anatomy of the small Intestine—a comparative anatomic study on humans and pigs
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Mühlenbruch, Volker Schumpelick, Uwe Klinge, Ulf P. Neumann, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small Intestine Perfusion. Methods Intestines were obtained from human cadavers ( n = 8; small Intestine, n = 51) and from pigs ( n = 10; small Intestine, n = 60). Vascularization was visualized with mennige gelatin Perfusion and high-resolution mammography. Endothelial cell density was analyzed with immunohistochemistry and factor VIII antibodies. We also investigated the influence of suture techniques (circular anastomoses, n = 19; longitudinal sutures, n = 15) on vascular Perfusion. Results Only human samples showed branching of mesenteric vessels. Compared to the pig, human vessels showed closer connections at the entrance to the bowel wall ( p = 0.045) and higher numbers of intramural anastomoses ( p
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Vascular anatomy of the small Intestine-a comparative anatomic study on humans and pigs.
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Mühlenbruch, Volker Schumpelick, Uwe Klinge, Ulf P. Neumann, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small Intestine Perfusion.
Min-sheng Chen - One of the best experts on this subject based on the ideXlab platform.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of Chromatography B, 2010Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Abstract Puerarin is a major active ingredient of Pueraria Radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID–MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1 × 100 mm, 1.8 μm) at 30 °C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min −1 , and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min −1 , dry temperature: 350 °C, nebulizer: 40 psi, capillary: −3500 V, scan range: 250–800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7- O -glucuronide and puerarin-4′- O -glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10–15 and 15–30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
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Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and Intestine of rats.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Puerarin is a major active ingredient of Pueraria radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID-MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1x100 mm, 1.8 microm) at 30 degrees C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min(-1), and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min(-1), dry temperature: 350 degrees C, nebulizer: 40 psi, capillary: -3500 V, scan range: 250-800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of puerarin (M1 and M2) were detected. M1 and M2 are presumed to be puerarin-7-O-glucuronide and puerarin-4'-O-glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of puerarin, the levels of M1 and M2 then reached their peaks at 10-15 and 15-30 min, respectively. The metabolic profiles were similar in rat liver and Intestine investigated by in situ liver and Intestine Perfusion, indicating that no metabolic regioselectivity of puerarin occurs in the two organs.
Klaus-thilo Von Trotha - One of the best experts on this subject based on the ideXlab platform.
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Vascular anatomy of the small Intestine—a comparative anatomic study on humans and pigs
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Mühlenbruch, Volker Schumpelick, Uwe Klinge, Ulf P. Neumann, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small Intestine Perfusion. Methods Intestines were obtained from human cadavers ( n = 8; small Intestine, n = 51) and from pigs ( n = 10; small Intestine, n = 60). Vascularization was visualized with mennige gelatin Perfusion and high-resolution mammography. Endothelial cell density was analyzed with immunohistochemistry and factor VIII antibodies. We also investigated the influence of suture techniques (circular anastomoses, n = 19; longitudinal sutures, n = 15) on vascular Perfusion. Results Only human samples showed branching of mesenteric vessels. Compared to the pig, human vessels showed closer connections at the entrance to the bowel wall ( p = 0.045) and higher numbers of intramural anastomoses ( p
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Vascular anatomy of the small Intestine-a comparative anatomic study on humans and pigs.
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Mühlenbruch, Volker Schumpelick, Uwe Klinge, Ulf P. Neumann, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small Intestine Perfusion.
