The Experts below are selected from a list of 444 Experts worldwide ranked by ideXlab platform
Julia M. Polak - One of the best experts on this subject based on the ideXlab platform.
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Differentiation of fetal osteoblasts and formation of mineralized bone nodules by 45S5 Bioglass?? conditioned medium in the absence of osteogenic supplements
Biomaterials, 2009Co-Authors: Olga Tsigkou, Julia M. Polak, Julian R. Jones, Molly M. StevensAbstract:Abstract Bioactive glasses bond strongly to bone in vivo and their Ionic Dissolution products have previously been shown to have stimulatory properties on adult and fetal osteoblasts and to induce the differentiation of embryonic stem cells towards the osteoblastic lineage in vitro. In the present study, the effect of 45S5 Bioglass® conditioned medium with two different Si concentrations (15 μg/ml (BGCM/15) and 20 μg/ml (BGCM/20)) on human fetal osteoblast growth, differentiation and extracellular matrix production and mineralization was investigated. In the first instance, primary fetal osteoblasts were examined for the osteoblast phenotypic markers alkaline phosphatase (ALP), collagen type I (Col I) and OB Cadherin (Cadherin 11) (OB Cad) as well as for the mesenchymal stem cell markers CD105 and CD166. At passage 0 more than 50% of the population was positive for Col I and ALP, but at passage 2, the proportion of cells expressing ALP increased. In addition at passage 0 more than 50% of the fetal osteoblasts expressed the mesenchymal stem cell surface markers CD105 and CD166. Treatment with BGCM/15 and BGCM/20 in the absence of osteogenic supplements increased the gene expression of the bone extracellular matrix proteins alkaline phosphatase, osteonectin and bone sialoprotein as determined by quantitative real time reverse transcriptase-polymerase chain reaction (rt RT-PCR) analysis. Extracellular matrix production was also enhanced in the absence of osteogenic supplements by the 45S5 Bioglass® conditioned medium as demonstrated by ALP enzymatic activity, osteocalcin and Col I protein synthesis. Furthermore, BGCM/15 and BGCM/20 significantly enhanced the formation of mineralized nodules, based on alizarin red histochemical staining, without necessitating the addition of β-glycerophosphate, l -ascorbate-2-phosphate or dexamethasone (commonly used osteogenic supplements).
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characterization of human fetal osteoblasts by microarray analysis following stimulation with 58s bioactive gel glass Ionic Dissolution products
Journal of Biomedical Materials Research Part B, 2006Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, releasing their constitutive ions in solution. There is evidence suggesting that these Ionic Dissolution products influence osteoblast-specific processes. Here, we investigated the effect of 58S sol–gel-derived bioactive glass (60 mol % SiO2, 36 mol % CaO, 4 mol % P2O5) Dissolution products on primary osteoblasts derived from human fetal long bone explant cultures (hFOBs). We used U133A human genome GeneChip® oligonucleotide arrays to examine 22,283 transcripts and variants, which represent over 18,000 well-substantiated human genes. Hybridization of samples (biotinylated cRNA) derived from monolayer cultures of hFOBs on the arrays revealed that 10,571 transcripts were expressed by these cells, with high confidence. These included transcripts representing osteoblast-related genes coding for growth factors and their associated molecules or receptors, protein components of the extracellular matrix (ECM), enzymes involved in degradation of the ECM, transcription factors, and other important osteoblast-associated markers. A 24-h treatment with a single dosage of Ionic products of sol–gel 58S Dissolution induced the differential expression of a number of genes, including IL-6 signal transducer/gp130, ISGF-3/STAT1, HIF-1 responsive RTP801, ERK1 p44 MAPK (MAPK3), MAPKAPK2, IGF-I and IGFBP-5. The over 2-fold up-regulation of gp130 and MAPK3 and down-regulation of IGF-I were confirmed by real-time RT-PCR analysis. These data suggest that 58S Ionic Dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
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Characterization of human fetal osteoblasts by microarray analysis following stimulation with 58S bioactive gel‐glass Ionic Dissolution products
Journal of Biomedical Materials Research Part B, 2006Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, releasing their constitutive ions in solution. There is evidence suggesting that these Ionic Dissolution products influence osteoblast-specific processes. Here, we investigated the effect of 58S sol–gel-derived bioactive glass (60 mol % SiO2, 36 mol % CaO, 4 mol % P2O5) Dissolution products on primary osteoblasts derived from human fetal long bone explant cultures (hFOBs). We used U133A human genome GeneChip® oligonucleotide arrays to examine 22,283 transcripts and variants, which represent over 18,000 well-substantiated human genes. Hybridization of samples (biotinylated cRNA) derived from monolayer cultures of hFOBs on the arrays revealed that 10,571 transcripts were expressed by these cells, with high confidence. These included transcripts representing osteoblast-related genes coding for growth factors and their associated molecules or receptors, protein components of the extracellular matrix (ECM), enzymes involved in degradation of the ECM, transcription factors, and other important osteoblast-associated markers. A 24-h treatment with a single dosage of Ionic products of sol–gel 58S Dissolution induced the differential expression of a number of genes, including IL-6 signal transducer/gp130, ISGF-3/STAT1, HIF-1 responsive RTP801, ERK1 p44 MAPK (MAPK3), MAPKAPK2, IGF-I and IGFBP-5. The over 2-fold up-regulation of gp130 and MAPK3 and down-regulation of IGF-I were confirmed by real-time RT-PCR analysis. These data suggest that 58S Ionic Dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
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dose and time dependent effect of bioactive gel glass Ionic Dissolution products on human fetal osteoblast specific gene expression
Journal of Biomedical Materials Research Part B, 2005Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Priya Saravanapavan, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, and release their constitutive ions into solution. There has been some evidence suggesting that these Ionic-Dissolution products influence osteoblast-specific processes. Here, the effect of 58S sol–gel-derived bioactive glass (60% SiO2, 36% CaO, 4% P2O5, in molar percentage) on primary osteoblasts derived from human fetal long bone explant cultures is investigated, and it is hypothesized that critical concentrations of sol–gel-Dissolution products (consisting of a combination of simple inorganic ions) can enhance osteoblast phenotype in vitro by affecting the expression of a number of genes associated with the differentiation and extracellular matrix deposition processes. Cells were exposed to a range of 58S dosages continuously for a period of 4–14 days in monolayer cultures. Quantitative real-time RT-PCR analysis of a panel of osteoblast-specific markers showed a varied gene expression pattern in response to the material. The highest concentration of Ca and Si tested (96 and 50 ppm, respectively) promoted upregulation of gene expression for most markers (including alkaline phosphatase, osteocalcin, and osteopontin) at the latest time point, compared to non-58S-treated control, although this observation was not statistically significant. The same 58S concentration produced higher ALP activity levels and increased proliferation throughout the culture period, compared to lower dosages tested; however, the results generated were again not statistically significant. The data overall suggest that no significant effect can be ascribed to the Ionic products of 58S bioactive gel-glass Dissolution tested here and their ability to stimulate osteoblastic marker gene expression. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
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Dose‐ and time‐dependent effect of bioactive gel‐glass Ionic‐Dissolution products on human fetal osteoblast‐specific gene expression
Journal of Biomedical Materials Research Part B, 2005Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Priya Saravanapavan, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, and release their constitutive ions into solution. There has been some evidence suggesting that these Ionic-Dissolution products influence osteoblast-specific processes. Here, the effect of 58S sol–gel-derived bioactive glass (60% SiO2, 36% CaO, 4% P2O5, in molar percentage) on primary osteoblasts derived from human fetal long bone explant cultures is investigated, and it is hypothesized that critical concentrations of sol–gel-Dissolution products (consisting of a combination of simple inorganic ions) can enhance osteoblast phenotype in vitro by affecting the expression of a number of genes associated with the differentiation and extracellular matrix deposition processes. Cells were exposed to a range of 58S dosages continuously for a period of 4–14 days in monolayer cultures. Quantitative real-time RT-PCR analysis of a panel of osteoblast-specific markers showed a varied gene expression pattern in response to the material. The highest concentration of Ca and Si tested (96 and 50 ppm, respectively) promoted upregulation of gene expression for most markers (including alkaline phosphatase, osteocalcin, and osteopontin) at the latest time point, compared to non-58S-treated control, although this observation was not statistically significant. The same 58S concentration produced higher ALP activity levels and increased proliferation throughout the culture period, compared to lower dosages tested; however, the results generated were again not statistically significant. The data overall suggest that no significant effect can be ascribed to the Ionic products of 58S bioactive gel-glass Dissolution tested here and their ability to stimulate osteoblastic marker gene expression. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
Aldo R Boccaccini - One of the best experts on this subject based on the ideXlab platform.
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45S5 AND 1393 BIOACTIVE GLASSES DIFFERENTIALLY REGULATE BEHAVIOUR AS WELL AS ANGIOGENIC AND OSTEOGENIC RESPONSE OF HUMAN MESENCHYMAL STROMAL CELLS
2018Co-Authors: Julia Catherine Berkmann, Aldo R Boccaccini, Taimoor H. Qazi, S Hafeez, Jochen Schmidt, Janosch Schoon, Sven Geissler, Georg N. Duda, Evi LippensAbstract:Promising work on bioactive glasses (BAGs) in bone defect regeneration has led to their clinical implementation. However, the effects of the Ionic Dissolution products of different types and the physical interaction modalities of BAGs on the behavior and function of mesenchymal stromal cells (MSCs) of human patients have not received sufficient attention. Recently, we showed that the in vitro response of hMSC to micron-sized, monodispersed BAGs is dependent on dosage, composition, and mode of interaction1. Two commercially available and widely used types of BAGs, namely the silicate BAGs 45S5 and 1393, were used to study hMSC cell behavior. Interestingly, exposure to 1393 BAG resulted in superior metabolic activity, proliferation, and cell spreading compared to 45S5 BAG in similar dosage, suggesting that additional cellular functions could also be differentially modulated by both glasses1. In the context of bone regeneration, the hMSCs’ potential to secrete angiogenic factors as well as deposit mineralize...
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Dosage and composition of bioactive glasses differentially regulate angiogenic and osteogenic response of human MSCs
Journal of Biomedical Materials Research Part A, 2018Co-Authors: Taimoor H. Qazi, Aldo R Boccaccini, Julia Catherine Berkmann, Janosch Schoon, Sven Geissler, Georg N. Duda, Evi LippensAbstract:Vascularization of the fracture site and cell-mediated deposition of the mineralized matrix are crucial determinants for successful bone regeneration after injury. Ceramic biomaterials such as bioactive glasses (BAGs) that release bioactive ions have shown promising results in bone defect regeneration. However, it remains unclear how the dosage and composition of bioactive ions influence the angiogenic and osteogenic behavior of primary human mesenchymal stromal cells (MSCs). Here, we show that exposure to Ionic Dissolution products from 1393 and 45S5 BAGs can evoke distinct angiogenic and osteogenic responses from primary MSCs in a dose- and composition-dependent manner. Significantly higher concentrations of the pro-angiogenic factors VEGF, HGF, PIGF, angiopoietin, and angiogenin were detected in conditioned media (CM) from MSCs exposed to 45S5, but not 1393, BAGs. Application of this CM to human umbilical vein endothelial cells (HUVECs) resulted in robust 2D tube formation in vitro. Osteogenic differentiation of MSCs was assessed by gene expression analysis and mineralization assays. Low concentrations (0.1% w/v) of 1393 BAGs significantly enhanced the gene expression of RUNX2 and ALP and induced an earlier onset of matrix mineralization compared to all other groups. We further tested whether simultaneous exposure to both BAGs would improve both angiogenic secretion and osteogenic differentiation of MSCs, and did not find evidence to support this hypothesis. Our results provide evidence of BAG composition-dependent enhancement of primary human MSCs' regenerative function, besides also underlining the importance of an in vitro evaluation of the dose-response relationship to translate BAG based approaches into safe and effective clinical therapies. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2827-2837, 2018., 2018.
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Influence of Dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells
Biomedical Glasses, 2017Co-Authors: Preethi Balasubramanian, Rainer Detsch, Leticia Esteban-tejeda, Alina Grünewald, José S. Moya, Aldo R BoccacciniAbstract:AbstractThis study evaluated the influence of Ionic Dissolution products of a novel Ca-enriched silicate bioactive glass compared to commercial available hydroxyapaptite samples (Endobonr) on cell activity and vascular endothelial growth factor (VEGF) release in vitro. Bone marrow stromal cells (ST-2) were cultivated with the supernatant of granules of different sizes and at different concentrations (0-1 wt/vol % of granules) for 48 h. In addition to in vitro studies, Ca-ion release from all as cell morphology observation revealed no cytotoxic effect of the released products from all tested materials. It was found that supernatants from granules in concentrations of 1 wt/vol %enhanced the VEGF release from ST2 cells, which is important as a marker of the vascularisation ability of the glass during the bone healing process.
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Angiogenic potential of boron-containing bioactive glasses: in vitro study
Journal of Materials Science, 2017Co-Authors: P. Balasubramanian, L. Hupa, B. Jokic, R. Detsch, A. Grünewald, Aldo R BoccacciniAbstract:Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the Ionic Dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO_2–Na_2O–K_2O–MgO–CaO–P_2O_5–B_2O_3 (with varying amounts of SiO_2 and B_2O_3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.
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in vitro human umbilical vein endothelial cells response to Ionic Dissolution products from lithium containing 45s5 bioactive glass
Materials, 2017Co-Authors: Luis Haro A Durand, Gabriela E Vargas, Rosa Veramesones, Aldo R Boccaccini, Maria Paola Zago, Alberto Baldi, Maria Fanovich, Alejandro A GorustovichAbstract:Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the Ionic Dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.
Larry L. Hench - One of the best experts on this subject based on the ideXlab platform.
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Chronology of Bioactive Glass Development and Clinical Applications
New Journal of Glass and Ceramics, 2020Co-Authors: Larry L. HenchAbstract:The key research and development steps for bioactive glass (45S5 Bioglass) are documented from the date of discovery in 1969 through FDA approvals of the first dental, ENT, maxillo-facial and orthopedic clinical products. Understanding the mechanisms and quantifying the rapid surface reactions to form a bone-bonding hydroxyl-carbonate apatite (HCA) layer on the bioactive glass in contact with living bone was a vital part of the early development of this class of biomaterials. A key later discovery was enhanced osteogenesis and in situ bone regeneration by controlled release of Ionic Dissolution products from the bioactive glass particulates that leads to up-regulation and activation of seven families of genes, a process called osteostimulation.
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Bioactive Glass: Chronology, Characterization, and Genetic Control of Tissue Regeneration
Springer Series in Biomaterials Science and Engineering, 2014Co-Authors: Larry L. HenchAbstract:This chapter reviews the discovery and chronology of the development of bioactive glasses and the recent findings that controlled release of biologically active Ca and Si ions from bioactive glasses leads to the upregulation and activation of seven families of genes in osteoprogenitor cells leading to rapid bone regeneration. This finding offers the possibility of creating a new generation of gene-activating glasses designed for patient-specific in situ regeneration of tissues. Studies also indicate that controlled release of lower concentrations of Ionic Dissolution products from bioactive glasses can be used to induce angiogenesis and thereby offer potential for design of gene-activating glasses for soft tissue and cardiovascular system regeneration.
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Genetic design of bioactive glass
Journal of the European Ceramic Society, 2009Co-Authors: Larry L. HenchAbstract:This paper reviews the discovery that controlled release of biologically active Ca and Si ions from bioactive glasses leads to the up-regulation and activation of seven families of genes in osteoprogenitor cells that give rise to rapid bone regeneration. This finding offers the possibility of creating a new generation of gene activating glasses designed specially for tissue engineering and in situ regeneration of tissues. Recent findings also indicate that controlled release of lower concentrations of Ionic Dissolution products from bioactive glasses can be used to induce angiogenesis and thereby offer potential for design of gene activating glasses for soft tissue regeneration. © 2008.
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Clinical Use of Bioactive Glasses for Maxillo-Facial Repair
Key Engineering Materials, 2008Co-Authors: Ian Thompson, Larry L. HenchAbstract:Autogenous bone grafts are considered to be the gold standard in maxillo-facial surgery. However, drawbacks of donor site morbidity and unpredictable rates of resorbtion often limit their use. In vivo tests have shown that 45S5 bioactive glass particles placed in critical size bone defects lead to regeneration of new bone that has the structural characteristics and architecture of mature trabecular bone. In vitro tests using primary osteoblast cultures have shown that the bioactive glass particles release Ionic Dissolution products that provide genetic stimuli that control osteoblast cell cycles and lead to rapid growth of mineralized bone nodules. These in vitro and in vivo results led to approval of use of bioactive glass particles and monolithic bioactive glass implants for use in maxillo-facial reconstructions after removal of bone cysts and trauma, as described by several case histories.
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characterization of human fetal osteoblasts by microarray analysis following stimulation with 58s bioactive gel glass Ionic Dissolution products
Journal of Biomedical Materials Research Part B, 2006Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, releasing their constitutive ions in solution. There is evidence suggesting that these Ionic Dissolution products influence osteoblast-specific processes. Here, we investigated the effect of 58S sol–gel-derived bioactive glass (60 mol % SiO2, 36 mol % CaO, 4 mol % P2O5) Dissolution products on primary osteoblasts derived from human fetal long bone explant cultures (hFOBs). We used U133A human genome GeneChip® oligonucleotide arrays to examine 22,283 transcripts and variants, which represent over 18,000 well-substantiated human genes. Hybridization of samples (biotinylated cRNA) derived from monolayer cultures of hFOBs on the arrays revealed that 10,571 transcripts were expressed by these cells, with high confidence. These included transcripts representing osteoblast-related genes coding for growth factors and their associated molecules or receptors, protein components of the extracellular matrix (ECM), enzymes involved in degradation of the ECM, transcription factors, and other important osteoblast-associated markers. A 24-h treatment with a single dosage of Ionic products of sol–gel 58S Dissolution induced the differential expression of a number of genes, including IL-6 signal transducer/gp130, ISGF-3/STAT1, HIF-1 responsive RTP801, ERK1 p44 MAPK (MAPK3), MAPKAPK2, IGF-I and IGFBP-5. The over 2-fold up-regulation of gp130 and MAPK3 and down-regulation of IGF-I were confirmed by real-time RT-PCR analysis. These data suggest that 58S Ionic Dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Alejandro A Gorustovich - One of the best experts on this subject based on the ideXlab platform.
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in vitro human umbilical vein endothelial cells response to Ionic Dissolution products from lithium containing 45s5 bioactive glass
Materials, 2017Co-Authors: Luis Haro A Durand, Gabriela E Vargas, Rosa Veramesones, Aldo R Boccaccini, Maria Paola Zago, Alberto Baldi, Maria Fanovich, Alejandro A GorustovichAbstract:Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the Ionic Dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.
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angiogenic effects of Ionic Dissolution products released from a boron doped 45s5 bioactive glass
Journal of Materials Chemistry B, 2015Co-Authors: Luis Haro A Durand, Gabriela E Vargas, Nelida Marcela Romero, Rosa Veramesones, Jose M Portolopez, Aldo R Boccaccini, Maria Paola Zago, Alberto Baldi, Alejandro A GorustovichAbstract:In regenerative medicine of vascularized tissues, there is a great interest in the use of biomaterials that are able to stimulate angiogenesis, a process necessary for rapid revascularization to allow the transport and exchange of oxygen, nutrients, growth factors and cells that take part in tissue repair and/or regeneration. An increasing number of publications have shown that bioactive glasses stimulate angiogenesis. Because it has been established that boron (B) may play a role in angiogenesis, the aim of this study was to assess the in vivo angiogenic effects of the Ionic Dissolution products that from a bioactive glass (BG) in the 45S5 system doped with 2 wt% B2O3 (45S5.2B). The pro-angiogenic capacity of 45S5.2B BG was assessed on the vasculature of the embryonic quail chorioallantoic membrane (CAM). Ionic Dissolution products from 45S5.2B BG increased angiogenesis. This is quantitatively evidenced by the greater expression of integrin αvβ3 and higher vascular density in the embryonic quail CAM. The response observed at 2 and 5 days post-treatment was equivalent to that achieved by applying 10 µg mL−1 of basic fibroblast growth factor. These results show that the Ionic Dissolution products released from the bioactive glass 45S5.2B stimulate angiogenesis in vivo. The effects observed are attributed to the presence the Ionic Dissolution products, which contained 160 ± 10 µM borate.
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in vitro endothelial cell response to Ionic Dissolution products from boron doped bioactive glass in the sio2 cao p2o5 na2o system
Journal of Materials Chemistry B, 2014Co-Authors: Luis Haro A Durand, Aldo R Boccaccini, Alberto Baldi, Adrian Gongora, Jose Porto M Lopez, Paola M Zago, Alejandro A GorustovichAbstract:As it has been established that boron (B) may perform functions in angiogenesis and osteogenesis, the controlled and localized release of B ions from bioactive glasses (BGs) is expected to provide a promising therapeutic alternative for regenerative medicine of vascularized tissues, such as bone. The aim of this study was to assess the in vitro angiogenic effects of the Ionic Dissolution products (IDPs) from BGs in the SiO2–CaO–Na2O–P2O5 (45S5) system and of those from 45S5 BG doped with 2 wt% B2O3 (45S5.2B). The results show, for the first time, the IDPs from 45S5.2B BG stimulated human umbilical vein endothelial cell (HUVEC) proliferation and migration that were associated with phosphorylation of extracellular signal-related kinase (ERK) 1/2, focal adhesion kinase (FAK) and p38 protein. It was also shown that IDPs from 45S5.2B BG could enhance in vitro HUVEC tubule formation and secretion of interleukin 6 (IL6) and the basic fibroblast growth factor (bFGF). The effects observed are attributed to the presence of B in the IDPs. These findings are relevant to bone tissue engineering and regeneration because the IDPs from 45S5.2B BG may act as inexpensive inorganic angiogenic agents providing a convenient alternative to the application of conventional angiogenic growth factors.
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In vitro endothelial cell response to Ionic Dissolution products from boron-doped bioactive glass in the SiO2–CaO–P2O5–Na2O system
Journal of Materials Chemistry B, 2014Co-Authors: Luis A. Haro Durand, Aldo R Boccaccini, Maria Paola Zago, Alberto Baldi, Adrian Gongora, José M. Porto López, Alejandro A GorustovichAbstract:As it has been established that boron (B) may perform functions in angiogenesis and osteogenesis, the controlled and localized release of B ions from bioactive glasses (BGs) is expected to provide a promising therapeutic alternative for regenerative medicine of vascularized tissues, such as bone. The aim of this study was to assess the in vitro angiogenic effects of the Ionic Dissolution products (IDPs) from BGs in the SiO2–CaO–Na2O–P2O5 (45S5) system and of those from 45S5 BG doped with 2 wt% B2O3 (45S5.2B). The results show, for the first time, the IDPs from 45S5.2B BG stimulated human umbilical vein endothelial cell (HUVEC) proliferation and migration that were associated with phosphorylation of extracellular signal-related kinase (ERK) 1/2, focal adhesion kinase (FAK) and p38 protein. It was also shown that IDPs from 45S5.2B BG could enhance in vitro HUVEC tubule formation and secretion of interleukin 6 (IL6) and the basic fibroblast growth factor (bFGF). The effects observed are attributed to the presence of B in the IDPs. These findings are relevant to bone tissue engineering and regeneration because the IDPs from 45S5.2B BG may act as inexpensive inorganic angiogenic agents providing a convenient alternative to the application of conventional angiogenic growth factors.
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In vitro Study of the Antibacterial Activity of Bioactive Glass-ceramic Scaffolds
Advanced Engineering Materials, 2009Co-Authors: Marta F. Gorriti, Aldo R Boccaccini, José M. Porto López, Carina Audisio, Alejandro A GorustovichAbstract:Staphylococcus aureus is an opportunistic pathogen of major clinical interest for its high prevalence in biomaterial-related infections. This experimental study provides the first evidence in vitro that bioactive glass-ceramic scaffolds made from both 45S5 Bioglass ® and from boron containing bioactive glass (45S5.2B) as well as their Ionic Dissolution products do no exhibit antibacterial effect against several strains of S. aureus.
Ioannis Christodoulou - One of the best experts on this subject based on the ideXlab platform.
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characterization of human fetal osteoblasts by microarray analysis following stimulation with 58s bioactive gel glass Ionic Dissolution products
Journal of Biomedical Materials Research Part B, 2006Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, releasing their constitutive ions in solution. There is evidence suggesting that these Ionic Dissolution products influence osteoblast-specific processes. Here, we investigated the effect of 58S sol–gel-derived bioactive glass (60 mol % SiO2, 36 mol % CaO, 4 mol % P2O5) Dissolution products on primary osteoblasts derived from human fetal long bone explant cultures (hFOBs). We used U133A human genome GeneChip® oligonucleotide arrays to examine 22,283 transcripts and variants, which represent over 18,000 well-substantiated human genes. Hybridization of samples (biotinylated cRNA) derived from monolayer cultures of hFOBs on the arrays revealed that 10,571 transcripts were expressed by these cells, with high confidence. These included transcripts representing osteoblast-related genes coding for growth factors and their associated molecules or receptors, protein components of the extracellular matrix (ECM), enzymes involved in degradation of the ECM, transcription factors, and other important osteoblast-associated markers. A 24-h treatment with a single dosage of Ionic products of sol–gel 58S Dissolution induced the differential expression of a number of genes, including IL-6 signal transducer/gp130, ISGF-3/STAT1, HIF-1 responsive RTP801, ERK1 p44 MAPK (MAPK3), MAPKAPK2, IGF-I and IGFBP-5. The over 2-fold up-regulation of gp130 and MAPK3 and down-regulation of IGF-I were confirmed by real-time RT-PCR analysis. These data suggest that 58S Ionic Dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
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Characterization of human fetal osteoblasts by microarray analysis following stimulation with 58S bioactive gel‐glass Ionic Dissolution products
Journal of Biomedical Materials Research Part B, 2006Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, releasing their constitutive ions in solution. There is evidence suggesting that these Ionic Dissolution products influence osteoblast-specific processes. Here, we investigated the effect of 58S sol–gel-derived bioactive glass (60 mol % SiO2, 36 mol % CaO, 4 mol % P2O5) Dissolution products on primary osteoblasts derived from human fetal long bone explant cultures (hFOBs). We used U133A human genome GeneChip® oligonucleotide arrays to examine 22,283 transcripts and variants, which represent over 18,000 well-substantiated human genes. Hybridization of samples (biotinylated cRNA) derived from monolayer cultures of hFOBs on the arrays revealed that 10,571 transcripts were expressed by these cells, with high confidence. These included transcripts representing osteoblast-related genes coding for growth factors and their associated molecules or receptors, protein components of the extracellular matrix (ECM), enzymes involved in degradation of the ECM, transcription factors, and other important osteoblast-associated markers. A 24-h treatment with a single dosage of Ionic products of sol–gel 58S Dissolution induced the differential expression of a number of genes, including IL-6 signal transducer/gp130, ISGF-3/STAT1, HIF-1 responsive RTP801, ERK1 p44 MAPK (MAPK3), MAPKAPK2, IGF-I and IGFBP-5. The over 2-fold up-regulation of gp130 and MAPK3 and down-regulation of IGF-I were confirmed by real-time RT-PCR analysis. These data suggest that 58S Ionic Dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
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dose and time dependent effect of bioactive gel glass Ionic Dissolution products on human fetal osteoblast specific gene expression
Journal of Biomedical Materials Research Part B, 2005Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Priya Saravanapavan, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, and release their constitutive ions into solution. There has been some evidence suggesting that these Ionic-Dissolution products influence osteoblast-specific processes. Here, the effect of 58S sol–gel-derived bioactive glass (60% SiO2, 36% CaO, 4% P2O5, in molar percentage) on primary osteoblasts derived from human fetal long bone explant cultures is investigated, and it is hypothesized that critical concentrations of sol–gel-Dissolution products (consisting of a combination of simple inorganic ions) can enhance osteoblast phenotype in vitro by affecting the expression of a number of genes associated with the differentiation and extracellular matrix deposition processes. Cells were exposed to a range of 58S dosages continuously for a period of 4–14 days in monolayer cultures. Quantitative real-time RT-PCR analysis of a panel of osteoblast-specific markers showed a varied gene expression pattern in response to the material. The highest concentration of Ca and Si tested (96 and 50 ppm, respectively) promoted upregulation of gene expression for most markers (including alkaline phosphatase, osteocalcin, and osteopontin) at the latest time point, compared to non-58S-treated control, although this observation was not statistically significant. The same 58S concentration produced higher ALP activity levels and increased proliferation throughout the culture period, compared to lower dosages tested; however, the results generated were again not statistically significant. The data overall suggest that no significant effect can be ascribed to the Ionic products of 58S bioactive gel-glass Dissolution tested here and their ability to stimulate osteoblastic marker gene expression. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
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Dose‐ and time‐dependent effect of bioactive gel‐glass Ionic‐Dissolution products on human fetal osteoblast‐specific gene expression
Journal of Biomedical Materials Research Part B, 2005Co-Authors: Ioannis Christodoulou, Larry L. Hench, Lee D K Buttery, Priya Saravanapavan, Julia M. PolakAbstract:Bioactive glasses dissolve upon immersion in culture medium, and release their constitutive ions into solution. There has been some evidence suggesting that these Ionic-Dissolution products influence osteoblast-specific processes. Here, the effect of 58S sol–gel-derived bioactive glass (60% SiO2, 36% CaO, 4% P2O5, in molar percentage) on primary osteoblasts derived from human fetal long bone explant cultures is investigated, and it is hypothesized that critical concentrations of sol–gel-Dissolution products (consisting of a combination of simple inorganic ions) can enhance osteoblast phenotype in vitro by affecting the expression of a number of genes associated with the differentiation and extracellular matrix deposition processes. Cells were exposed to a range of 58S dosages continuously for a period of 4–14 days in monolayer cultures. Quantitative real-time RT-PCR analysis of a panel of osteoblast-specific markers showed a varied gene expression pattern in response to the material. The highest concentration of Ca and Si tested (96 and 50 ppm, respectively) promoted upregulation of gene expression for most markers (including alkaline phosphatase, osteocalcin, and osteopontin) at the latest time point, compared to non-58S-treated control, although this observation was not statistically significant. The same 58S concentration produced higher ALP activity levels and increased proliferation throughout the culture period, compared to lower dosages tested; however, the results generated were again not statistically significant. The data overall suggest that no significant effect can be ascribed to the Ionic products of 58S bioactive gel-glass Dissolution tested here and their ability to stimulate osteoblastic marker gene expression. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
