The Experts below are selected from a list of 6747 Experts worldwide ranked by ideXlab platform
Makoto Yoshimoto - One of the best experts on this subject based on the ideXlab platform.
-
carotenoids with a 5 6 dihydro 5 6 dihydroxy β end group from yellow sweet potato benimasari Ipomoea Batatas lam
Phytochemistry, 2007Co-Authors: Takashi Maoka, Koji Ishiguro, Naoshige Akimoto, Masaru Yoshinaga, Makoto YoshimotoAbstract:Abstract A series of carotenoids with a 5,6-dihydro-5,6-dihydroxy-β-end group, named Ipomoeaxanthins A ( 1 ), B ( 2 ), C1 ( 3 ) and C2 ( 4 ) were isolated from the flesh of yellow sweet potato “Benimasari”, Ipomoea Batatas L am . Their structures were determined to be (5 R ,6 S ,3′ R )-5,6-dihydro-β,β-carotene-5,6,3′-triol ( 1 ), (5 R ,6 S ,5′ R ,6′ S )-5,6,5′,6′-tetrahydro-β,β-carotene-5,6,5′6′-tetrol ( 2 ), (5 R ,6 S ,5′ R ,8′ R )-5′,8′-epoxy-5,6,5′,8′-tetrahydro-β,β-carotene-5,6-diol ( 3 ), and (5 R ,6 S ,5′R,8′ S )-5′,8′-epoxy-5,6,5′,8′-tetrahydro-β,β-carotene-5,6-diol ( 4 ) by UV–Vis, NMR, MS and CD data.
-
growth suppression of human cancer cells by polyphenolics from sweetpotato Ipomoea Batatas l leaves
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Rie Kurata, Osamu Yamakawa, Masaru Adachi, Makoto YoshimotoAbstract:Sweetpotato leaves (Ipomoea Batatas L.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. The human cancer cells used in this research included a stomach cancer (Kato III), a colon cancer (DLD-1), and a promyelocytic leukemia cell (HL-60). Caffeic acid and di- and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against HL-60 cells than other di- and tricaffeoylquinic acids. In attempting to clarify the mechanism of growth suppression with the addition of the apoptotic inhibitor N-ethylmaleimide, we observed that the nuclear granulation in 3,4,5-tri-O-caffeoylquinic acid-treated HL-60 cells suggested apoptosis induction. This effect was confirmed by DNA fragmentation, an increase of caspase-3 activity, and expression of c-Jun. Growth suppression of HL-60 cells by 3,4,5-tri-O-caffeoylquinic acid was determined to be the result of apoptotic death of the cells. These results indicate that 3,4,5-tri-O-caffeoylquinic acid may have potential for cancer prevention.
-
characterization of acylated anthocyanins in callus induced from storage root of purple fleshed sweet potato Ipomoea Batatas l
BioMed Research International, 2004Co-Authors: Norihiko Terahara, Makoto Yoshimoto, Izabela Konczak, H Ono, Osamu YamakawaAbstract:Four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (Ipomoea Batatas L) cultivar Ayamurasaki. The anthocyanins were respectively identified as cyanidin 3-O-(2-O-(6-O-(E)-caffeoyl-β-D-glucopyranosyl)-β-D-glucopyranoside) -5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-caffeoyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)- 5-O-β-D-glucopyranoside, and peonidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside by chemical and spectroscopic analyses. These anthocyanins were examined with respect to the stability in neutral aqueous solution as well as the radical scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. These acylated anthocyanins exhibited both higher stability and higher DPPH radical scavenging activity than corresponding nonacylated cyanidin and peonidin 3-O-sophoroside-5-O-glucosides.
-
antimutagenicity of mono di and tricaffeoylquinic acid derivatives isolated from sweetpotato Ipomoea Batatas l leaf
Bioscience Biotechnology and Biochemistry, 2002Co-Authors: Makoto Yoshimoto, Md Shahidul Islam, Shoji Yahara, Shigenori Okuno, Koji Ishiguro, Osamu YamakawaAbstract:The caffeoylquinic acid derivatives, 3-mono-O-caffeoylquinic acid (chlorogenic acid, ChA), 3,4-di-O-caffeoylquinic acid (3,4-diCQA), 3,5-di-O-caffeoylquinic acid (3,5-diCQA), 4,5-di-O-caffeoylquinic acid (4,5-diCQA) and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-triCQA), and caffeic acid (CA) were isolated from the sweetpotato (Ipomoea Batatas L.) leaf. We examined the antimutagenicity of these caffeoylquinic acid compounds to promote new uses of the sweetpotato leaf. These caffeoylquinic acid derivatives effectively inhibited the reverse mutation induced by Trp-P-1 on Salmonella typhimurium TA 98. The antimutagenicity of these derivatives was 3,4,5-triCQA>3,4-diCQA=3,5-diCQA=4,5-diCQA>ChA in this order. There was no difference in the antimutagenicity of all dicaffeoylquinic acid derivatives. A comparison of the activities and structures of these compounds suggested that the number of caffeoyl groups bound to quinic acid played a role in the antimutagenicity of the caffeoylquinic acid derivatives. The sweetpo...
-
identification and characterization of foliar polyphenolic composition in sweetpotato Ipomoea Batatas l genotypes
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Md Shahidul Islam, Makoto Yoshimoto, Shoji Yahara, Shigenori Okuno, And Koji Ishiguro, Osamu YamakawaAbstract:Trials over two years were conducted using 1389 sweetpotato (Ipomoea Batatas L.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. Wide variation was observed in relation to their total and individual leaf polyphenolic constituents. In all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. The six different polyphenolic compounds were identified and quantified by NMR, FABMS, and RPHPLC analysis procedures. This is the first report of polyphenolic compositions in sweetpotato leaves. The relative levels of polyphenolic acids in sweetpotato leaves were as follows: 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > chlorogenic acid (3-O-caffeoylquinic acid) > 3,4-di-O-caffeoylquinic acid > 3,4,5-tri-O-caffeoylquinic acid > caffeic acid. The highest 3,4,5-tri-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid occurred at 221 and 1183.30 mg/100 g dry weight, respectively. ...
Osamu Yamakawa - One of the best experts on this subject based on the ideXlab platform.
-
growth suppression of human cancer cells by polyphenolics from sweetpotato Ipomoea Batatas l leaves
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Rie Kurata, Osamu Yamakawa, Masaru Adachi, Makoto YoshimotoAbstract:Sweetpotato leaves (Ipomoea Batatas L.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. The human cancer cells used in this research included a stomach cancer (Kato III), a colon cancer (DLD-1), and a promyelocytic leukemia cell (HL-60). Caffeic acid and di- and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against HL-60 cells than other di- and tricaffeoylquinic acids. In attempting to clarify the mechanism of growth suppression with the addition of the apoptotic inhibitor N-ethylmaleimide, we observed that the nuclear granulation in 3,4,5-tri-O-caffeoylquinic acid-treated HL-60 cells suggested apoptosis induction. This effect was confirmed by DNA fragmentation, an increase of caspase-3 activity, and expression of c-Jun. Growth suppression of HL-60 cells by 3,4,5-tri-O-caffeoylquinic acid was determined to be the result of apoptotic death of the cells. These results indicate that 3,4,5-tri-O-caffeoylquinic acid may have potential for cancer prevention.
-
characterization of acylated anthocyanins in callus induced from storage root of purple fleshed sweet potato Ipomoea Batatas l
BioMed Research International, 2004Co-Authors: Norihiko Terahara, Makoto Yoshimoto, Izabela Konczak, H Ono, Osamu YamakawaAbstract:Four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (Ipomoea Batatas L) cultivar Ayamurasaki. The anthocyanins were respectively identified as cyanidin 3-O-(2-O-(6-O-(E)-caffeoyl-β-D-glucopyranosyl)-β-D-glucopyranoside) -5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-caffeoyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)- 5-O-β-D-glucopyranoside, and peonidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside by chemical and spectroscopic analyses. These anthocyanins were examined with respect to the stability in neutral aqueous solution as well as the radical scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. These acylated anthocyanins exhibited both higher stability and higher DPPH radical scavenging activity than corresponding nonacylated cyanidin and peonidin 3-O-sophoroside-5-O-glucosides.
-
absorption of acylated anthocyanins in rats and humans after ingesting an extract of Ipomoea Batatas purple sweet potato tuber
Bioscience Biotechnology and Biochemistry, 2004Co-Authors: Katsuhisa Harada, Osamu Yamakawa, Mitsuyoshi Kano, Tomomi Takayanagi, Fumiyasu IshikawaAbstract:We evaluated the absorbability of anthocyanins in humans and rats administered with a beverage prepared from an extract of the tuber of purple sweet potato (Ipomoea Batatas Cultivar Ayamurasaki), or with an anthocyanin concentrate. Two major anthocyanin components, cyanidin 3-O-(2-O-(6-O-(E)-caffeoyl-β-D-glucopyranosyl)-β-D-glucopyranoside)-5-O-β-D-glucopyranoside) and peonidin 3-O-(2-O-(6-O-(E)-caffeoyl-β-D-glucopyranosyl)-β-D-glucopyranoside)-5-O-β-D-glucopyranoside), were detected in the plasma and urine of both rats and humans by HPLC or liquid chromatography/mass spectrometry (LC/MS). The plasma concentration of anthocyanins in humans reached a maximum 90 minutes after ingestion, and the recovery of anthocyanins in the urine was estimated as 0.01–0.03%. These results indicate that acylated anthocyanins could be selectively absorbed after ingesting food.
-
antimutagenicity of mono di and tricaffeoylquinic acid derivatives isolated from sweetpotato Ipomoea Batatas l leaf
Bioscience Biotechnology and Biochemistry, 2002Co-Authors: Makoto Yoshimoto, Md Shahidul Islam, Shoji Yahara, Shigenori Okuno, Koji Ishiguro, Osamu YamakawaAbstract:The caffeoylquinic acid derivatives, 3-mono-O-caffeoylquinic acid (chlorogenic acid, ChA), 3,4-di-O-caffeoylquinic acid (3,4-diCQA), 3,5-di-O-caffeoylquinic acid (3,5-diCQA), 4,5-di-O-caffeoylquinic acid (4,5-diCQA) and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-triCQA), and caffeic acid (CA) were isolated from the sweetpotato (Ipomoea Batatas L.) leaf. We examined the antimutagenicity of these caffeoylquinic acid compounds to promote new uses of the sweetpotato leaf. These caffeoylquinic acid derivatives effectively inhibited the reverse mutation induced by Trp-P-1 on Salmonella typhimurium TA 98. The antimutagenicity of these derivatives was 3,4,5-triCQA>3,4-diCQA=3,5-diCQA=4,5-diCQA>ChA in this order. There was no difference in the antimutagenicity of all dicaffeoylquinic acid derivatives. A comparison of the activities and structures of these compounds suggested that the number of caffeoyl groups bound to quinic acid played a role in the antimutagenicity of the caffeoylquinic acid derivatives. The sweetpo...
-
identification and characterization of foliar polyphenolic composition in sweetpotato Ipomoea Batatas l genotypes
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Md Shahidul Islam, Makoto Yoshimoto, Shoji Yahara, Shigenori Okuno, And Koji Ishiguro, Osamu YamakawaAbstract:Trials over two years were conducted using 1389 sweetpotato (Ipomoea Batatas L.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. Wide variation was observed in relation to their total and individual leaf polyphenolic constituents. In all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. The six different polyphenolic compounds were identified and quantified by NMR, FABMS, and RPHPLC analysis procedures. This is the first report of polyphenolic compositions in sweetpotato leaves. The relative levels of polyphenolic acids in sweetpotato leaves were as follows: 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > chlorogenic acid (3-O-caffeoylquinic acid) > 3,4-di-O-caffeoylquinic acid > 3,4,5-tri-O-caffeoylquinic acid > caffeic acid. The highest 3,4,5-tri-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid occurred at 221 and 1183.30 mg/100 g dry weight, respectively. ...
Irene Dini - One of the best experts on this subject based on the ideXlab platform.
-
saponins in Ipomoea Batatas tubers isolation characterization quantification and antioxidant properties
Food Chemistry, 2009Co-Authors: Irene Dini, Gian Carlo Tenore, Afshin DiniAbstract:Triterpene saponins are a class of plant natural products with a wide range of bioactivities, which makes them an interesting research subject. This work reports, for the first time, the isolation and characterization of saponins in Ipomoea Batatas tuber flour, their quantification and antioxidant properties. Their structures were characterized on the basis of UV, FAB–MS, ESI–MS, GC–MS, polarimetry and NMR data, as: oleanolic acid-3-O-[β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-β-d-glucuronopyranosyl]-28-O-β-d-glucopyranoside (sandrosaponin IX) (1) and oleanolic acid-3-O-[β-d-galactopyranosyl-(1→3)-β-d-glucuronopyranosyl]-28-O-β-d-glucopyranoside (2). A new quantitative HPLC–DAD method for saponin content determination in this tuber was developed and validated. Their total content was 200.01 mg/100 g dry weight (RSD = 7.2%; p < 0.001). The single saponin contents were: 161.20 mg/100 g dry weight (RSD = 0.58%; p < 0.001) for saponin 1 and 14.67 mg/100 g dry weight (RSD = 0.41%; p < 0.001) for saponin 2. The antioxidant activities, tested by DPPH and FRAP assay, of total phytochemical fraction and of single saponins were moderate in relation to commercial standards.
-
saponins in Ipomoea Batatas tubers isolation characterization quantification and antioxidant properties
Food Chemistry, 2009Co-Authors: Irene Dini, Gian Carlo Tenore, Afshin DiniAbstract:Abstract Triterpene saponins are a class of plant natural products with a wide range of bioactivities, which makes them an interesting research subject. This work reports, for the first time, the isolation and characterization of saponins in Ipomoea Batatas tuber flour, their quantification and antioxidant properties. Their structures were characterized on the basis of UV, FAB–MS, ESI–MS, GC–MS, polarimetry and NMR data, as: oleanolic acid-3-O-[β- d -glucopyranosyl-(1→2)-β- d -galactopyranosyl-(1→2)-β- d -glucuronopyranosyl]-28-O-β- d -glucopyranoside (sandrosaponin IX) (1) and oleanolic acid-3-O-[β- d -galactopyranosyl-(1→3)-β- d -glucuronopyranosyl]-28-O-β- d -glucopyranoside (2). A new quantitative HPLC–DAD method for saponin content determination in this tuber was developed and validated. Their total content was 200.01 mg/100 g dry weight (RSD = 7.2%; p
-
New polyphenol derivative in Ipomoea Batatas tubers and its antioxidant activity.
Journal of Agricultural and Food Chemistry, 2006Co-Authors: Irene Dini, Gian Carlo Tenore, Antonio DiniAbstract:Four different polyphenolic compounds were isolated by chromatographic methods from methanolic and hydromethanolic extracts of Ipomoea Batatas tuber flour. On the basis of UV, mass, and NMR analysis procedures, the structure of the isolated compounds were determined as 4,5-di-O-caffeoyldaucic acid (1), 4-O-caffeoylquinic acid (2), 3,5-di-O-caffeoylquinic acid (3), and 1,3-di-O-caffeoylquinic acid (4). To the best of our knowledge, this is the first report of isolation and characterization of compound 1. Then, we evaluated the antioxidant activity of daucic acid derivative by using DPPH and FRAP methods together with authentic antioxidant standards, l-ascorbic acid, tert-butyl-4-hydroxy toluene (BHT), and gallic acid. The activity of compound 1 in both methods was higher than that of all standards used at the same molar concentration. Keywords: Camote; sweet potato; Ipomoea Batatas; caffeoyl; convolvulaceae; NMR; antioxidant
Sánchez López, Diana Beatriz - One of the best experts on this subject based on the ideXlab platform.
-
Azotobacter chroococcum y Azospirillum lipoferum como bioestimulantes en cultivo de Ipomoea Batatas Lam.
2019Co-Authors: Sánchez López, Diana Beatriz, Pérez Pazos, Jazmín Vanessa, Luna Castellanos, Lily Lorena, García Peña, Joaquín Alfonso, Espitia Montes, Amaury AroldoAbstract:Introduction. The excessive use of nitrogen fertilizers in sweet potato crops contributes to the ecosystems contamination; to reduce this effect and improve crop productivity, the incorporation of plant growth promoting rhizobacteria (PGPRs) in the strategies of crop managment constitute a sustainbale tool. Objective. The objective of this research was to incorporate the bacterial strains Azotobacter chroococcum IBCR19 and Azospirillum lipoferum IBSC7 in the nitrogen fertilization, and to evaluate their effect on the yield and bromatological composition of sweet potato tuberous roots (Ipomoea Batatas Lam). Materials and methods. The study was made in the municipality of Corozal (Sucre, Colombia) during the months of June to October of 2017. An experimental area of 840 m2 was used where twenty-four plots with apical cuttings of the 15020078 accession were established, and distributed under a completely randomized design with an increased factorial arrangement (3x2+2). Root dry matter, yield, and bromatological composition of sweet potato roots were evaluated. Results. Fresh yield and root dry matter showed significant differences (p≤0.05) among the treatments, where the application of A. chroococcum IBCR19 and 75% of nitrogen fertilization reached the highest average values of yield and dry matter of 12.18 t.ha-1 y 2.92 t.ha-1, respectively. Similarly, the protein and ethereal extract concentrations differed significantly (p≤0.05) between the inoculated treatments in relation to the absolute control. Conclusion. Based on the results obtained, it can be inferred that the inoculation with A. chroococcum IBCR19 reduced the nitrogen fertilization levels by 25% and constitutes a promising strain as a biostimulant.Introducción. La utilización excesiva de fertilizantes nitrogenados en cultivos de batata contribuye con la contaminación de los ecosistemas, para disminuir este efecto y mejorar la productividad del cultivo, la incorporación, de rizobacterias promotoras de crecimiento vegetal (PGPRs, por sus siglas en inglés) en las estrategias de manejo del cultivo, constituyen una herramienta sostenible. Objetivo. El objetivo de esta investigación fue incorporar las cepas bacterianas Azotobacter chroococcum IBCR19 y Azospirillum lipoferum IBSC7 en la fertilización nitrogenada y evaluar su efecto en el rendimiento y composición bromatológica de las raíces tuberosas de batata (Ipomoea Batatas Lam). Materiales y métodos. El estudio se realizó en el municipio de Corozal (Sucre, Colombia), durante los meses de junio a octubre del 2017. Se utilizó un área experimental de 840 m2, donde se establecieron veinticuatro parcelas con esquejes apicales de la accesión 15020078, distribuidas bajo un diseño completamente aleatorizado con arreglo factorial aumentado (3x2+2). Se evaluó materia seca radicular, rendimiento y composición bromatológica de las raíces de batata. Resultados. El rendimiento fresco y de materia seca radicular presentó diferencias significativas (p≤0,05) entre los tratamientos, en donde la aplicación de A. chroococcum IBCR19 y un 75 % de fertilización nitrogenada alcanzó los mayores valores medios de rendimiento y materia seca radicular 12,18 t.ha-1 y 2,92 t.ha-1, respectivamente. De igual forma, las concentraciones de proteína y extracto etéreo difirieron significativamente (p≤0,05) entre los tratamientos inoculados con relación al testigo absoluto. Conclusión. Con base en los resultados obtenidos se puede inferir que la inoculación con A. chroococcum IBCR19 logró reducir en un 25 % los niveles de fertilización nitrogenada y constituye una cepa promisoria como bioestimulante
-
Caracterización y efecto de Azotobacter, Azospirillum y Pseudomonas asociadas a Ipomoea Batatas del Caribe Colombiano
Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología, 2017Co-Authors: Pérez Pazos, Jazmín Vanessa, Sánchez López, Diana BeatrizAbstract:El uso de Rizobacterias promotoras de crecimiento vegetal (PGPR, por sus siglas en ingles) constituye una alternativa al uso de fertilizantes químicos favoreciendo el rendimiento de los cultivos. La presente investigación tuvo como objetivo la búsqueda, selección y caracterización de PGPR de los géneros Azotobacter, Azospirillum y Pseudomonas nativas de la rizósfera de cultivos de Ipomoea Batatas de zonas productoras representativas del Caribe Colombiano. Los aislados seleccionados se caracterizaron molecularmente y realizaron pruebas de solubilización de fósforo, producción de índoles y reducción de acetileno. Las cepas fueron probadas a nivel de invernadero en plántulas de Ipomoea Batatas producidas in vitro en las que se evaluó la altura, longitud radicular, masa seca de la parte aérea y radicular. Asociada a la rizósfera de Ipomoea Batatas se obtuvieron cepas de Azotobacter vinelandii, Azotobacter chroococcum, Azospirillum lipoferum, Azospirillum brasilense y Pseudomonas denitrificans, las cepas fueron capaces de solubilizar fósforo, producir índoles y reducir acetileno. Se obtuvo incrementos en parámetros de crecimiento como longitud radicular, altura, peso seco aéreo y radicular en plántulas de Ipomoea Batatas en invernadero con la inoculación de las bacterias seleccionadas frente a plántulas sin inocular. Los resultados catalogan a los aislados obtenidos como posibles microorga-nismos con potencial como biofertilizantes en batata.The use of plant growth promoting Rhizobacteria (PGPR) is an alternative to replace chemical fertilizers for the cultivation of agricultural crops. The aim of this research was to search, selection and characterization of PGPR from the genus Azotobacter, Azospirillum and Pseudomonas natives from sweet potato (Ipomoea Batatas) plants and rhizosphere of representative production regions of the Colombian Caribbean. Selected isolates were identified by molecular methods and they were screened in vitro for activities related to plant growth such as phosphate solubilization, indole production and acetylene reduction. The strains were tested in the greenhouse on plants of Ipomoea Batatas produced in vitro. The height, root length, dry mass of the shot and root were evaluated. Associated with sweet potato crop us finded strains identificated as Azotobacter vinelandii, Azotobacter chroococcum, Azospirillum lipoferum, Azospirillum brasilense and Pseudomonas denitrificans. The strains were able to solubilize phosphate, synthesize indole-3-acetic acid (IAA) and reduce acetylene. The inoculation of bacteria selected increased growth parameters such as root length, height, dry weight root and shoot in plants of sweet potato in greenhouse. Those results catalog to the isolated obtained as possible microorganisms with potential as biofertilizers in sweet potato
Amaury Aroldo Espitiamontes - One of the best experts on this subject based on the ideXlab platform.
-
azotobacter chroococcum y azospirillum lipoferum como bioestimulantes en cultivo de Ipomoea Batatas lam
Agronomía Mesoamericana, 2019Co-Authors: Diana Beatriz Sanchezlopez, Jazmin Vanessa Perezpazos, Lily Lorena Lunacastellanos, Joaquin Alfonso Garciapena, Amaury Aroldo EspitiamontesAbstract:espanolIntroduccion. La utilizacion excesiva de fertilizantes nitrogenados en cultivos de batata contribuye con la contaminacion de los ecosistemas, para disminuir este efecto y mejorar la productividad del cultivo, la incorporacion, de rizobacterias promotoras de crecimiento vegetal (PGPRs, por sus siglas en ingles) en las estrategias de manejo del cultivo, constituyen una herramienta sostenible. Objetivo. El objetivo de esta investigacion fue incorporar las cepas bacterianas Azotobacter chroococcum IBCR19 y Azospirillum lipoferum IBSC7 en la fertilizacion nitrogenada y evaluar su efecto en el rendimiento y composicion bromatologica de las raices tuberosas de batata (Ipomoea Batatas Lam). Materiales y metodos. El estudio se realizo en el municipio de Corozal (Sucre, Colombia), durante los meses de junio a octubre del 2017. Se utilizo un area experimental de 840 m2, donde se establecieron veinticuatro parcelas con esquejes apicales de la accesion 15020078, distribuidas bajo un diseno completamente aleatorizado con arreglo factorial aumentado (3x2+2). Se evaluo materia seca radicular, rendimiento y composicion bromatologica de las raices de batata. Resultados. El rendimiento fresco y de materia seca radicular presento diferencias significativas (p≤0,05) entre los tratamientos, en donde la aplicacion de A. chroococcum IBCR19 y un 75 % de fertilizacion nitrogenada alcanzo los mayores valores medios de rendimiento y materia seca radicular 12,18 t.ha-1 y 2,92 t.ha-1, respectivamente. De igual forma, las concentraciones de proteina y extracto etereo difirieron significativamente (p≤0,05) entre los tratamientos inoculados con relacion al testigo absoluto. Conclusion. Con base en los resultados obtenidos se puede inferir que la inoculacion con A. chroococcum IBCR19 logro reducir en un 25 % los niveles de fertilizacion nitrogenada y constituye una cepa promisoria como bioestimulante. EnglishIntroduction. The excessive use of nitrogen fertilizers in sweet potato crops contributes to the ecosystems contamination; to reduce this effect and improve crop productivity, the incorporation of plant growth promoting rhizobacteria (PGPRs) in the strategies of crop managment constitute a sustainbale tool. Objective. The objective of this research was to incorporate the bacterial strains Azotobacter chroococcum IBCR19 and Azospirillum lipoferum IBSC7 in the nitrogen fertilization, and to evaluate their effect on the yield and bromatological composition of sweet potato tuberous roots (Ipomoea Batatas Lam). Materials and methods. The study was made in the municipality of Corozal (Sucre, Colombia) during the months of June to October of 2017. An experimental area of 840 m2 was used where twenty-four plots with apical cuttings of the 15020078 accession were established, and distributed under a completely randomized design with an increased factorial arrangement (3x2+2). Root dry matter, yield, and bromatological composition of sweet potato roots were evaluated. Results. Fresh yield and root dry matter showed significant differences (p≤0.05) among the treatments, where the application of A. chroococcum IBCR19 and 75% of nitrogen fertilization reached the highest average values of yield and dry matter of 12.18 t.ha-1 y 2.92 t.ha-1, respectively. Similarly, the protein and ethereal extract concentrations differed significantly (p≤0.05) between the inoculated treatments in relation to the absolute control. Conclusion. Based on the results obtained, it can be inferred that the inoculation with A. chroococcum IBCR19 reduced the nitrogen fertilization levels by 25% and constitutes a promising strain as a biostimulant.
