The Experts below are selected from a list of 15480 Experts worldwide ranked by ideXlab platform
Sangmo Kwon - One of the best experts on this subject based on the ideXlab platform.
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3d printed complex tissue construct using stem cell laden decellularized extracellular matrix bioinks for cardiac repair
Biomaterials, 2017Co-Authors: Jinah Jang, Hunjun Park, Ju Young Park, Soo Jin Na, Moon Nyeo Park, Seung Hyun Choi, Sun Hwa Park, Sangmo KwonAbstract:Stem cell therapy is a promising therapeutic method for the treatment of Ischemic Heart Diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.
Jinah Jang - One of the best experts on this subject based on the ideXlab platform.
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customized 3d printed occluders enabling the reproduction of consistent and stable Heart failure in swine models
Bio-Design and Manufacturing, 2021Co-Authors: Han B Kim, Jinah Jang, Seungman Jung, Hyukjin Park, Doo Sun Sim, Munki Kim, Sanskrita Das, Youngkeun Ahn, Myung H Jeong, Young Jun HongAbstract:Reproducibility of clinical output is important when investigating therapeutic efficacy in pre-clinical animal studies. Due to its physiological relevance, a swine myocardial infarction (MI) model has been widely used to evaluate the effectiveness of stem cells or tissue-engineered constructs for Ischemic Heart Diseases. Several methods are used to induce MI in the swine model. However, it is difficult, using these approaches, to obtain a similar level of functional outcomes from a group of animals due to interpersonal variation, leading to increased experimental cost. Hence, in order to minimize human intervention, we developed an approach to use a customized occluder that has dimensional similarities with that of the coronary artery of animals in the case of the swine model. We carried out angiography to measure the diameter of the middle left anterior descending artery of each individual animal to fabricate the customized occluder using a 3D-printing system. The fabricated occluder contained a central hole smaller than that of the targeted middle left anterior descending artery to mimic an atherosclerotic coronary artery that has an approximately 20% blocked condition. Interestingly, the 3D-printed occluder can provide continuous blood flow through the central pore, indicating a high survival rate (88%) of up to 28 days post-operation. This method showed the possibility of creating consistent myocardial infarction induction as compared to the conventional representative closed-chest method (50% survival rate), thus highlighting how our method can have a profound effect on accelerating reliable experiments for developing new therapeutic approaches to Ischemic Heart Diseases.
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3d printed complex tissue construct using stem cell laden decellularized extracellular matrix bioinks for cardiac repair
Biomaterials, 2017Co-Authors: Jinah Jang, Hunjun Park, Ju Young Park, Soo Jin Na, Moon Nyeo Park, Seung Hyun Choi, Sun Hwa Park, Sangmo KwonAbstract:Stem cell therapy is a promising therapeutic method for the treatment of Ischemic Heart Diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.
Wei Zhu - One of the best experts on this subject based on the ideXlab platform.
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a novel human long noncoding rna scdal promotes angiogenesis through snf5 mediated gdf6 expression
Advanced Science, 2021Co-Authors: Huan Chen, Yingchao Wang, Changchen Xiao, Lin Fan, Zhiwei Zhong, Xiaoying Chen, Shuhan Zhong, Yanna Shi, Jinghai Chen, Wei ZhuAbstract:Angiogenesis is essential for vascular development. The roles of regulatory long noncoding RNAs (lncRNAs) in mediating angiogenesis remain under-explored. Human embryonic stem cell-derived mesenchymal stem cells (hES-MSCs) are shown to exert more potent cardioprotective effects against cardiac ischemia than human bone marrow-derived MSCs (hBM-MSCs), associated with enhanced neovascularization. The purpose of this study is to search for angiogenic lncRNAs enriched in hES-MSCs, and investigate their roles and mechanisms. AC103746.1 is one of the most highly expressed intergenic lncRNAs detected in hES-MSCs versus hBM-MSCs, and named as SCDAL (stem cell-derived angiogenic lncRNA). SCDAL knockdown significantly reduce the angiogenic potential and reparative effects of hES-MSCs in the infarcted Hearts, while overexpression of SCDAL in either hES-MSCs or hBM-MSCs exhibits augmented angiogenesis and cardiac function recovery. Mechanistically, SCDAL induces growth differentiation factor 6 (GDF6) expression via direct interaction with SNF5 at GDF6 promoter. Secreted GDF6 promotes endothelial angiogenesis via non-canonical vascular endothelial growth factor receptor 2 activation. Furthermore, SCDAL-GDF6 is expressed in human endothelial cells, and directly enhances endothelial angiogenesis in vitro and in vivo. Thus, these findings uncover a previously unknown lncRNA-dependent regulatory circuit for angiogenesis. Targeted intervention of the SCDAL-GDF6 pathway has potential as a therapy for Ischemic Heart Diseases.
Sun Hwa Park - One of the best experts on this subject based on the ideXlab platform.
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3d printed complex tissue construct using stem cell laden decellularized extracellular matrix bioinks for cardiac repair
Biomaterials, 2017Co-Authors: Jinah Jang, Hunjun Park, Ju Young Park, Soo Jin Na, Moon Nyeo Park, Seung Hyun Choi, Sun Hwa Park, Sangmo KwonAbstract:Stem cell therapy is a promising therapeutic method for the treatment of Ischemic Heart Diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.
Seung Hyun Choi - One of the best experts on this subject based on the ideXlab platform.
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3d printed complex tissue construct using stem cell laden decellularized extracellular matrix bioinks for cardiac repair
Biomaterials, 2017Co-Authors: Jinah Jang, Hunjun Park, Ju Young Park, Soo Jin Na, Moon Nyeo Park, Seung Hyun Choi, Sun Hwa Park, Sangmo KwonAbstract:Stem cell therapy is a promising therapeutic method for the treatment of Ischemic Heart Diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.
