The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform
Frank G. Zalom - One of the best experts on this subject based on the ideXlab platform.
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sequencing of tuta absoluta genome to develop snp genotyping assays for species identification
Journal of Pest Science, 2019Co-Authors: Christine A Tabuloc, Kyle M Lewald, William R Conner, Alexander B Cain, Kristine E Godfrey, Judit Arno, Nuria Agusti, Clerison Regis Perini, Jerson Vanderlei Carus Guedes, Frank G. ZalomAbstract:Tuta absoluta is one of the most devastating pests of fresh market and processing tomatoes. Native to South America, its detection was confined to that continent until 2006 when it was identified in Spain. It has now spread to almost every continent, threatening countries whose economies rely heavily on tomatoes. This insect causes damage to all developmental stages of its host plant, leading to crop losses as high as 80–100%. Although T. absoluta has yet to be found in the USA and China, which makes up a large portion of the tomato production in the world, computer models project a high likelihood of invasion. To halt the continued spread of T. absoluta and limit economic loss associated with tomato supply chain, it is necessary to develop accurate and efficient methods to identify T. absoluta and strengthen surveillance programs. Current identification of T. absoluta relies on examination of morphology and assessment of host plant damage, which are difficult to differentiate from that of native tomato pests. To address this need, we sequenced the genomes of T. absoluta and two closely related Gelechiidae, Keiferia lycopersicella and Phthorimaea operculella, and developed a bioinformatic pipeline to design a panel of 21-SNP markers for species identification. The accuracy of the SNP panel was validated in a multiplex format using the iPLEX chemistry of Agena MassARRAY system. Finally, the new T. absoluta genomic resources we generated can be leveraged to study T. absoluta biology and develop species-specific management strategies.
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Insect fragments in processed tomatoes
Journal of Economic Entomology, 1994Co-Authors: Frank G. Zalom, Allan JonesAbstract:Undamaged tomato fruit batches were adulterated with either lab-reared Spodoptera exigua larvae, Keiferia lycopersicella larvae, or damaged fruit (some containing larvae) collected from several California inspection stations at densities equivalent to 0, 1,2,3,4, and 10% infestations. Commercial processing procedures were used to process tomatoes in 22.7-kg batches at the University of California, Davis, pilot plant and to can them as juice. Two screen sizes, 0.0825-cm mesh ‘juice’ screens and 0,15-cm ‘paste’ screens, were used during processing for comparison. The cans were opened, and 200-ml samples were removed and examined for larval fragments and insect fragments other than Lepidoptera larvae. Regression analysis of percentage infested tomatoes before processing and number of larval fragments in 200-ml samples of the resulting processed tomato product indicated a positive and significant linear relationship for all contaminants added and screen sizes. No positive relationship was observed for those hatches with K. lycopersicella larvae. The lowest residual root mean square values were obtained for the batches in which damaged fruit was added. Low levels of insect fragments were found in most samples including those which were not infested with Lepidoptera larvae, indicating that natural infestations of other insects can result in some fragments in the processed product and that the paste and juice screens currently used for processing tomatoes do not remove all fragments.
J. T. Trumble - One of the best experts on this subject based on the ideXlab platform.
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Resistance in wild tomatoes to larvae of a specialist herbivore, Keiferia lycopersicella
2015Co-Authors: S. Y. H. Lin, J. T. TrumbleAbstract:glandular trichomes, tomato Delayed larval development, higher mortality, and reduced leaf consumption by the tomato pinworm, Keiferia lycopersicella (Walsingham) (Lepidoptera: Gelechiidae), were observed on two related wild tomatoes, Lycopersicon hirsutum Humb. & Bonpl. (LA 361) and L. hirsutum f. glabratum C. M. Mull (PI 134417), when compared with the commercial variety, L. esculentum cv. VFN 7718. The exudate of type VI trichomes of these wild accessions provided significant antibiotic effects against the first larval instar of K. lycopersicel-la: 95-97.5 ~ mortality occurred on foliage with the trichomes intact, versus 5.6-30 % mortality on foliage where trichome heads were removed. Basal cells of type VI trichomes and other epidermal cells as well as most of the palisade mesophyll cells of L. hirsutum f. glabratum, were not consumed by K. lycopersicella larvae, suggesting these structures also contain deterrent chemicals. Similar results were not evident for L. esculentum and L. hirsutum
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Estimating resistance to methomyl in the tomato pinworm (Lepidoptera: Gelechiidae) using a pheromone trap bioassay.
Crop Protection, 1996Co-Authors: David J. Schuster, Michael J. Brewer, B. Alvarado-rodriguez, K.a. Sorensen, J. T. TrumbleAbstract:Abstract The tomato pinworm, Keiferia lycopersicella (Walsingham), is an important pest of tomatoes in the southern and southwestern United States and Mexico. A field-based method for assessing resistance of adults to the carbamate insecticide, methomyl, was developed by incorporating varying doses of technical insecticide into the adhesive of pheromone traps. The mortalities of male and female adults were similar whether exposed to methomyl, either by topical or pheromone trap bioassays. Larvae were less susceptible than adults to methomyl by topical application. Therefore, adult male susceptibility provides a good estimate of adult female susceptibility but may overestimate larval susceptibility. Nevertheless, using the pheromone trap bioassay and making comparisons of field populations with a laboratory strain suggested that all field populations evaluated were less susceptible to methomyl than the laboratory strain. Populations in Mexico were more susceptible than populations in California, where methomyl is used more often. Populations in Florida were either intermediate in susceptibility or were similar to those in California.
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Development and economic evaluation of an IPM program for fresh market tomato production in Mexico
Agriculture Ecosystems & Environment, 1993Co-Authors: J. T. Trumble, Benito Alvarado-rodriguezAbstract:Abstract An integrated pest management (IPM) program based on intensive sampling, parasite releases, use of the mating disruption technique, and applications of microbial pesticides and abamectin was developed for the fresh market tomato industry in Sinaloa, Mexico. The IPM program for tomatoes was compared with conventional practices and an unmanaged control in each of three major agricultural valleys in autumn and winter crops, and in two valleys for spring plantings. The amount of marketable fruit production was similar for all treatments in the autumn plantings, but significantly higher in the IPM program during the winter and spring plantings. The densities of Liriomyza sativae Blanchard and Keiferia lycopersicella (Walsingham) eggs, larvae and adults were substantially reduced in the IPM treatment. Percent fruit damage by Spodoptera exigua (Hubner), Heliocoverpa zea (Boddie) and Heliothis virescens (Fabricius) was generally higher in the IPM treatment (4.2–10.9%) as compared with the conventional treatment (0.9–3.7%). However, the percent fruit damage by K. lycopersicella was significantly reduced in the IPM treatments (6.49–30.4%) vs. the conventional treatments (4.65–84.2%) in the winter and spring plantings. Net profits (value of fruit at harvest minus the cost of control) were substantially higher in the lower input IPM plots than in conventional treatments. In the autumn, net profits ranged from US$304 to US$579 ha−1 higher in the IPM treatment for carton values of US$5–US$11, respectively. In the winter and spring plantings, only the IPM approach was profitable. The IPM program offers substantial, long-term benefits in comparison with the conventional approach. Not only was the cost of the IPM program considerably less, but it: (1) reduced the potential for pesticide resistance development; (2) reduced the possibility of potential mammalian toxicity or non-target effects by using less toxic pesticides that are specific in activity; (3) provided less chance for fruit contamination or environmental damage.
Christine A Tabuloc - One of the best experts on this subject based on the ideXlab platform.
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sequencing of tuta absoluta genome to develop snp genotyping assays for species identification
Journal of Pest Science, 2019Co-Authors: Christine A Tabuloc, Kyle M Lewald, William R Conner, Alexander B Cain, Kristine E Godfrey, Judit Arno, Nuria Agusti, Clerison Regis Perini, Jerson Vanderlei Carus Guedes, Frank G. ZalomAbstract:Tuta absoluta is one of the most devastating pests of fresh market and processing tomatoes. Native to South America, its detection was confined to that continent until 2006 when it was identified in Spain. It has now spread to almost every continent, threatening countries whose economies rely heavily on tomatoes. This insect causes damage to all developmental stages of its host plant, leading to crop losses as high as 80–100%. Although T. absoluta has yet to be found in the USA and China, which makes up a large portion of the tomato production in the world, computer models project a high likelihood of invasion. To halt the continued spread of T. absoluta and limit economic loss associated with tomato supply chain, it is necessary to develop accurate and efficient methods to identify T. absoluta and strengthen surveillance programs. Current identification of T. absoluta relies on examination of morphology and assessment of host plant damage, which are difficult to differentiate from that of native tomato pests. To address this need, we sequenced the genomes of T. absoluta and two closely related Gelechiidae, Keiferia lycopersicella and Phthorimaea operculella, and developed a bioinformatic pipeline to design a panel of 21-SNP markers for species identification. The accuracy of the SNP panel was validated in a multiplex format using the iPLEX chemistry of Agena MassARRAY system. Finally, the new T. absoluta genomic resources we generated can be leveraged to study T. absoluta biology and develop species-specific management strategies.
Wendell L. Roelofs - One of the best experts on this subject based on the ideXlab platform.
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Identification of sex pheromone of tomato pinworm,Keiferia lycopersicella (Wals.).
Journal of chemical ecology, 1991Co-Authors: Ralph E. Charlton, John R. Mclaughlin, J. A. Wyman, Wendell L. RoelofsAbstract:A sex pheromone produced by femaleKeiferia lycopersicella (Walsingham) was isolated and identified as (E)-4-tridecenyl acetate, based on chemical analyses, electroantennogram assays, and field trapping in California and Florida. Males were captured equally well in traps baited with (E)-4-tridecenyl acetate alone or a variety of (Z)- and (E)-4-tridecenyl acetate blends, although theZ isomer was not detected in extracts of female glands.
Alexander B Cain - One of the best experts on this subject based on the ideXlab platform.
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sequencing of tuta absoluta genome to develop snp genotyping assays for species identification
Journal of Pest Science, 2019Co-Authors: Christine A Tabuloc, Kyle M Lewald, William R Conner, Alexander B Cain, Kristine E Godfrey, Judit Arno, Nuria Agusti, Clerison Regis Perini, Jerson Vanderlei Carus Guedes, Frank G. ZalomAbstract:Tuta absoluta is one of the most devastating pests of fresh market and processing tomatoes. Native to South America, its detection was confined to that continent until 2006 when it was identified in Spain. It has now spread to almost every continent, threatening countries whose economies rely heavily on tomatoes. This insect causes damage to all developmental stages of its host plant, leading to crop losses as high as 80–100%. Although T. absoluta has yet to be found in the USA and China, which makes up a large portion of the tomato production in the world, computer models project a high likelihood of invasion. To halt the continued spread of T. absoluta and limit economic loss associated with tomato supply chain, it is necessary to develop accurate and efficient methods to identify T. absoluta and strengthen surveillance programs. Current identification of T. absoluta relies on examination of morphology and assessment of host plant damage, which are difficult to differentiate from that of native tomato pests. To address this need, we sequenced the genomes of T. absoluta and two closely related Gelechiidae, Keiferia lycopersicella and Phthorimaea operculella, and developed a bioinformatic pipeline to design a panel of 21-SNP markers for species identification. The accuracy of the SNP panel was validated in a multiplex format using the iPLEX chemistry of Agena MassARRAY system. Finally, the new T. absoluta genomic resources we generated can be leveraged to study T. absoluta biology and develop species-specific management strategies.