The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Stephen C. Pflugfelder - One of the best experts on this subject based on the ideXlab platform.
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The cornea in Keratoconjunctivitis Sicca.
Experimental eye research, 2020Co-Authors: Stephen C. Pflugfelder, Michael E. SternAbstract:The lacrimal functional unit (LFU) regulates tear production, composition, distribution and clearance to maintain a stable protective tear layer that is essential for maintaining corneal epithelial health. Dysfunction of the LFU, commonly referred to as dry eye, leads to increased tear osmolarity and levels of inflammatory mediators in tears that cause ocular surface epithelial disease, termed Keratoconjunctivitis Sicca (KCS). Corneal changes in KCS include glycocalyx loss, barrier disruption, surface irregularity inflammatory cytokine/chemokine production, cornification and apoptosis. These can reduce visual function and the increased shear force on the corneal epithelium can stimulate nociceptors sensitized by inflammation causing irritation and pain that may precede frank clinical signs. Therapy of Keratoconjunctivitis Sicca should be tailored to improve tear stability, normalize tear composition, improve barrier function and minimize shear forces and damaging inflammation to improve corneal epithelial health.
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suppression of th1 mediated Keratoconjunctivitis Sicca by lifitegrast
Journal of Ocular Pharmacology and Therapeutics, 2018Co-Authors: Rodrigo G De Souza, Stephen C. Pflugfelder, Michael E. Stern, Cintia S De PaivaAbstract:Abstract Purpose: Increased interferon gamma (IFN-γ) expression in dry eye causes ocular surface epithelial disease termed Keratoconjunctivitis Sicca (KCS). The purpose of this study was to investi...
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topical cyclosporine inhibits conjunctival epithelial apoptosis in experimental murine Keratoconjunctivitis Sicca
Cornea, 2005Co-Authors: Bradley Strong, Michael E. Stern, William J Farley, Stephen C. PflugfelderAbstract:Purpose:Increased apoptosis in the conjunctival epithelium has been observed in experimental murine Keratoconjunctivitis Sicca (KCS). Topical cyclosporine (CsA) has been noted to reduce conjunctival epithelial apoptosis in chronic canine and human KCS. The purpose of this study is to determine if to
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increased expression of the type 1 growth factor receptor family in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca
American Journal of Ophthalmology, 2000Co-Authors: Maria E Carvajal, Zuguo Liu, Coralie Carothers A Carraway, Kermit L Carraway, Stephen C. PflugfelderAbstract:Abstract PURPOSE: To investigate the expression of type 1 growth factor receptors (epidermal growth factor receptor, ErbB2, and ErbB3) in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca. METHODS: Immunofluorescent staining and Western blotting were performed to grade the level of expression of the epidermal growth factor receptor ErbB2, and ErbB3 in conjunctival epithelial impression cytologies taken from both eyes of seven normal subjects and 22 patients with Keratoconjunctivitis Sicca. RESULTS: Epidermal growth factor receptor staining was observed in a greater percentage of Keratoconjunctivitis Sicca than normal samples ( P P P P P P P P P CONCLUSION: The expression of the type 1 growth factor receptors is significantly greater in the conjunctival epithelium of eyes with Keratoconjunctivitis Sicca than normal eyes. The increased expression of these receptors was positively correlated with ocular surface dye staining. The increased expression of these receptors may contribute to the abnormal growth and differentiation of the conjunctival epithelium that occurs in Keratoconjunctivitis Sicca.
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Increased expression of the type 1 growth factor receptor family in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca.
American journal of ophthalmology, 2000Co-Authors: Zuguo Liu, Maria E Carvajal, Kermit L Carraway, Coralie A. Carothers Carraway, Stephen C. PflugfelderAbstract:To investigate the expression of type 1 growth factor receptors (epidermal growth factor receptor, ErbB2, and ErbB3) in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca. Immunofluorescent staining and Western blotting were performed to grade the level of expression of the epidermal growth factor receptor ErbB2, and ErbB3 in conjunctival epithelial impression cytologies taken from both eyes of seven normal subjects and 22 patients with Keratoconjunctivitis Sicca. Epidermal growth factor receptor staining was observed in a greater percentage of Keratoconjunctivitis Sicca than normal samples (P <.05). ErbB2 and ErB3 staining in the apical conjunctival epithelium was observed in both groups, but stronger ErbB2 and ErbB3 staining was noted in Keratoconjunctivitis Sicca conjunctival samples (P <.05). The relative levels of expression of these receptor proteins on immunoblots were consistent with immunofluorescent staining. On immunoblots, epidermal growth factor receptor protein was detected in 50% of Keratoconjunctivitis Sicca samples, but none of the normal samples (P <.025). The expression of ErbB2 and ErbB3 on immunoblots was also greater in the Keratoconjunctivitis Sicca samples (P <.05). Immunofluorescent staining scores for these receptors were correlated with conjunctival lissamine green staining scores (r =. 574, P <.01 for epidermal growth factor receptor; r =.620, P <.0025 for ErbB2; r =.502, P <.025 for ErbB3) and with corneal fluorescein staining (r =.409, P <.05 for ErbB2; r =.588, P <.005 for ErbB3). The expression of the type 1 growth factor receptors is significantly greater in the conjunctival epithelium of eyes with Keratoconjunctivitis Sicca than normal eyes. The increased expression of these receptors was positively correlated with ocular surface dye staining. The increased expression of these receptors may contribute to the abnormal growth and differentiation of the conjunctival epithelium that occurs in Keratoconjunctivitis Sicca.
Zuguo Liu - One of the best experts on this subject based on the ideXlab platform.
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increased expression of the type 1 growth factor receptor family in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca
American Journal of Ophthalmology, 2000Co-Authors: Maria E Carvajal, Zuguo Liu, Coralie Carothers A Carraway, Kermit L Carraway, Stephen C. PflugfelderAbstract:Abstract PURPOSE: To investigate the expression of type 1 growth factor receptors (epidermal growth factor receptor, ErbB2, and ErbB3) in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca. METHODS: Immunofluorescent staining and Western blotting were performed to grade the level of expression of the epidermal growth factor receptor ErbB2, and ErbB3 in conjunctival epithelial impression cytologies taken from both eyes of seven normal subjects and 22 patients with Keratoconjunctivitis Sicca. RESULTS: Epidermal growth factor receptor staining was observed in a greater percentage of Keratoconjunctivitis Sicca than normal samples ( P P P P P P P P P CONCLUSION: The expression of the type 1 growth factor receptors is significantly greater in the conjunctival epithelium of eyes with Keratoconjunctivitis Sicca than normal eyes. The increased expression of these receptors was positively correlated with ocular surface dye staining. The increased expression of these receptors may contribute to the abnormal growth and differentiation of the conjunctival epithelium that occurs in Keratoconjunctivitis Sicca.
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Increased expression of the type 1 growth factor receptor family in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca.
American journal of ophthalmology, 2000Co-Authors: Zuguo Liu, Maria E Carvajal, Kermit L Carraway, Coralie A. Carothers Carraway, Stephen C. PflugfelderAbstract:To investigate the expression of type 1 growth factor receptors (epidermal growth factor receptor, ErbB2, and ErbB3) in the conjunctival epithelium of patients with Keratoconjunctivitis Sicca. Immunofluorescent staining and Western blotting were performed to grade the level of expression of the epidermal growth factor receptor ErbB2, and ErbB3 in conjunctival epithelial impression cytologies taken from both eyes of seven normal subjects and 22 patients with Keratoconjunctivitis Sicca. Epidermal growth factor receptor staining was observed in a greater percentage of Keratoconjunctivitis Sicca than normal samples (P <.05). ErbB2 and ErB3 staining in the apical conjunctival epithelium was observed in both groups, but stronger ErbB2 and ErbB3 staining was noted in Keratoconjunctivitis Sicca conjunctival samples (P <.05). The relative levels of expression of these receptor proteins on immunoblots were consistent with immunofluorescent staining. On immunoblots, epidermal growth factor receptor protein was detected in 50% of Keratoconjunctivitis Sicca samples, but none of the normal samples (P <.025). The expression of ErbB2 and ErbB3 on immunoblots was also greater in the Keratoconjunctivitis Sicca samples (P <.05). Immunofluorescent staining scores for these receptors were correlated with conjunctival lissamine green staining scores (r =. 574, P <.01 for epidermal growth factor receptor; r =.620, P <.0025 for ErbB2; r =.502, P <.025 for ErbB3) and with corneal fluorescein staining (r =.409, P <.05 for ErbB2; r =.588, P <.005 for ErbB3). The expression of the type 1 growth factor receptors is significantly greater in the conjunctival epithelium of eyes with Keratoconjunctivitis Sicca than normal eyes. The increased expression of these receptors was positively correlated with ocular surface dye staining. The increased expression of these receptors may contribute to the abnormal growth and differentiation of the conjunctival epithelium that occurs in Keratoconjunctivitis Sicca.
Helmut Von Domarus - One of the best experts on this subject based on the ideXlab platform.
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microvascular submandibular gland transfer for severe cases of Keratoconjunctivitis Sicca
Plastic and Reconstructive Surgery, 2000Co-Authors: Peter Sieg, Gerd Geerling, Hartwig Kosmehl, Isabel Lauer, Kirsten Warnecke, Helmut Von DomarusAbstract:Free submandibular salivary gland transfer was investigated as a surgical method for the treatment of severe Keratoconjunctivitis Sicca. In an animal model, we examined the tolerance of warm ischemia of the submandibular gland. After temporary interruption of the blood supply (1 to 6 hours), the mor
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Microvascular submandibular gland transfer for severe cases of Keratoconjunctivitis Sicca.
Plastic and reconstructive surgery, 2000Co-Authors: Peter Sieg, Gerd Geerling, Hartwig Kosmehl, Isabel Lauer, Kirsten Warnecke, Helmut Von DomarusAbstract:Free submandibular salivary gland transfer was investigated as a surgical method for the treatment of severe Keratoconjunctivitis Sicca. In an animal model, we examined the tolerance of warm ischemia of the submandibular gland. After temporary interruption of the blood supply (1 to 6 hours), the morphologic changes in the submandibular gland were analyzed histologically and immunohistochemically in 41 rabbits. From 1.5 hours ischemia onward, an increasing structural damage of the parenchyma with emphasis on the secretory cells was seen. Six hours of ischemia caused total necrosis of the salivary gland. Our clinical experience includes 24 highly selected patients suffering from Keratoconjunctivitis Sicca, in whom we transferred 31 autologous submandibular glands to the temple for permanent autologous tear substitution within the past 4 years. The glands were implanted into a pocket prepared in the temporalis muscle, and the nourishing vessels were anastomosed to the superficial temporal artery and vein. The submandibular duct was implanted into the upper lateral conjunctival fornix. The transferred glands were left denervated. In addition to the clinical examination, scintigraphy with Tc 99m pertechnetate was used to document the graft's viability after the transfer. Viable incorporation with longstanding secretory function occurred in 26 of the 30 transplanted denervated salivary glands. The resulting lubrication of the treated eyes was irregular for up to 3 months in almost even case. One year after surgery, all patients with a viable transplant developed at least occasional epiphora, which was surgically managed by reducing the size of the graft in 10 patients. No severe side effects were seen in this series. The ophthalmologic evaluation of the method included the assessment of dry eye symptoms and of the volume and quality of ocular lubrication (Schirmer test, fluorescein break-up time), the pathology of the ocular surface (rose bengal staining), and the need for pharmaceutical tear substitutes. One year after surgery, 18 of 27 cases assessed were judged as significantly improved by these tests.
M S Norn - One of the best experts on this subject based on the ideXlab platform.
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treatment of Keratoconjunctivitis Sicca with liquid paraffin or polyvinyl alcohol in double blind trials
Acta Ophthalmologica, 2009Co-Authors: M S NornAbstract:Twenty-two eyes affected with Keratoconjunctivitis Sicca have been subjected to double trials using the double-blind randomized cross-over technique. Polyvinyl alcohol in a 5% concentration improved the condition whereas liquid paraffin aggravated it, as estimated subjectively, and by the measurement of the break up time of the corneal film, and, finally, by the scoring of rose bengal vital staining of exposed cornea and conjunctiva. The conclusion is drawn that oil is contraindicated in Keratoconjunctivitis Sicca, and that rose bengal scoring (modified Bijsterveld method) is a valuable aid in control of the Sicca syndrome.
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TREATMENT OF Keratoconjunctivitis Sicca WITH LIQUID PARAFFIN OR POLYVINYL ALCOHOL IN DOUBLE‐BLIND TRIALS
Acta ophthalmologica, 2009Co-Authors: M S NornAbstract:Twenty-two eyes affected with Keratoconjunctivitis Sicca have been subjected to double trials using the double-blind randomized cross-over technique. Polyvinyl alcohol in a 5% concentration improved the condition whereas liquid paraffin aggravated it, as estimated subjectively, and by the measurement of the break up time of the corneal film, and, finally, by the scoring of rose bengal vital staining of exposed cornea and conjunctiva. The conclusion is drawn that oil is contraindicated in Keratoconjunctivitis Sicca, and that rose bengal scoring (modified Bijsterveld method) is a valuable aid in control of the Sicca syndrome.
Robert Laibovitz - One of the best experts on this subject based on the ideXlab platform.
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pilot trial of cyclosporine 1 ophthalmic ointment in the treatment of Keratoconjunctivitis Sicca
Cornea, 1993Co-Authors: Robert Laibovitz, Suzanne Solch, Kim Andriano, Marianne Oconnell, Michael SilvermanAbstract:This trial was a randomized, double-masked, crossover study during which patients with Keratoconjunctivitis Sicca underwent 6 weeks of treatment with either cyclosporine 1% ophthalmic ointment or placebo followed by 6 weeks of the alternative treatment. Washout periods using only unpreserved artific
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Pilot Trial of Cyclosporine 1% Ophthalmic Ointment in the Treatment of Keratoconjunctivitis Sicca
Cornea, 1993Co-Authors: Robert Laibovitz, Suzanne Solch, Kim Andriano, Marianne O'connell, Michael H. SilvermanAbstract:This trial was a randomized, double-masked, crossover study during which patients with Keratoconjunctivitis Sicca underwent 6 weeks of treatment with either cyclosporine 1% ophthalmic ointment or placebo followed by 6 weeks of the alternative treatment. Washout periods using only unpreserved artificial tears preceded both treatment cycles. Twenty-five patients completed the first treatment period, but only eight met entry criteria for period II. Cyclosporine ointment was associated with initial mild to moderate redness, itching, and burning that returned to baseline levels within 1-2 weeks. Rose Bengal results and results of four subjective (patient diary) efficacy parameters favored cyclosporine: foreign body sensation, overall symptoms, hours of symptom control per day, and overall effectiveness. No systemic adverse events or laboratory abnormalities occurred. We conclude that (a) the crossover design is inappropriate for studying this disease; (b) mild to moderate itching, redness, and burning occur initially with cyclosporine administration, although tolerance quickly develops; (c) cyclosporine appears to benefit the ocular surface in Keratoconjunctivitis Sicca; and (d) further trials in this syndrome are warranted.
