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Hasan Mehraj - One of the best experts on this subject based on the ideXlab platform.
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evaluation of bottle gourd Lagenaria siceraria to growth and yield
Social Science Research Network, 2014Co-Authors: Afm Jamal Uddin, M I Tahidul, Md Shah Newaz Chowdhury, Ibne Haque Shiam, Hasan MehrajAbstract:An experiment was conducted at Horticulture farm, Sher-e-Bangla Agricultural University to evaluate the growth and yield performance of eleven (L1 to L11) bottle gourd lines during the period from November 2013 to March 2014. Maximum vine length (6.8 m), leaf area (975.4 cm2), number of fruit (14.3), fruit length (54.9 cm), single fruit weight (1.43 kg), yield/plant (20.6 kg), yield/plot (82.0 kg) and yield/ha (50.1 ton) was found from L11 followed by L10. On the other hand minimum sex ratio (male to female) (0.21), days to first male flower appearance (37.3) and female flower appearance (41.0) was found from L.
P G Yeole - One of the best experts on this subject based on the ideXlab platform.
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antihyperlipidemic effect of the methanolic extract from Lagenaria siceraria stand fruit in hyperlipidemic rats
Journal of Ethnopharmacology, 2009Co-Authors: B V Ghule, Mahavir H. Ghante, A N Saoji, P G YeoleAbstract:Ethnopharmacological relevance Lagenaria siceraria Stand. (Cucurbitaceae) fruits have been used, traditionally, in the treatment of hyperlipidemia and atherosclerotic impasse and considered as cardioprotective and cardiotonic drug. Aim of the study The present work was undertaken to investigate the effects of methanolic extract from Lagenaria siceraria in experimentally induced hyperlipidemia in rats. Materials and methods Methanolic extract of Lagenaria siceraria fruits (LSFE) (100, 200 and 300 mg/kg; p.o.) was administered to the high fat-diet-induced hyperlipidemic rats for 30 days to evaluate its antihyperlipidemic activity. Atorvastatin (10 mg/kg; p.o.) was used as a standard drug. Results At the 30th day, most significant reduction in lipid levels in the LSFE treated rats as compared to the rats fed with high-fat diet at the 0th day were: total cholesterol 290.14 ± 18.42 mg/dl vs. 228.58 ± 16.38 mg/dl, low-density lipoprotein cholesterol 195.14 ± 8.86 mg/dl vs. 120.57 ± 8.11 mg/dl, triglyceride 232.41 ± 15.22 mg/dl vs. 181.79 ± 15.68 mg/dl, very low-density lipoprotein cholesterol 46.48 ± 3.04 mg/dl vs. 36.35 ± 3.13 mg/dl (P < 0.0001). Conversely, high-density lipoprotein cholesterol levels were significantly (P < 0.0001) increased from 48.52 ± 6.52 to 71.66 ± 5.14 mg/dl. The increase in weight in rats administered with LSFE was less when compared to rats fed with high-fat diet. Moreover, LSFE also exhibited significant increase in excretion of bile acids. Conclusion The results demonstrate that the LSFE has a definite antihyperlipidemic potential. There is also a valid scientific basis for consuming it in the treatment of coronary artery diseases in India.
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diuretic activity of Lagenaria siceraria fruit extracts in rats
Indian Journal of Pharmaceutical Sciences, 2007Co-Authors: B V Ghule, Mahavir H. Ghante, P G Yeole, A N SaojiAbstract:Vacuum dried juice extract and methanol extract of the fruits of Lagenaria siceraria Mol. have been evaluated for its diuretic activity in albino rats. Different parameters viz. total urine volume (corrected for water intake during the test period), urine concentration of electrolytes such as sodium, potassium and chloride have been evaluated. The rats treated with vacuum dried Lagenaria siceraria juice extract (LSJE) and Lagenaria siceraria methanol extract (LSME) (100-200 mg/kg; p.o. ) showed higher urine volume when compared to the respective control. Both LSJE and LSME have exhibited dose-dependent increase in the excretion of electrolytes when compared to control group. The elevated diuretic potential of LSFE and LSME was statistically significant ( P <0.05) and comparable to that of the standard diuretic agent furosemide (20 mg/kg; i.p.).
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hypolipidemic and antihyperlipidemic effects of Lagenaria siceraria mol fruit extracts
Indian Journal of Experimental Biology, 2006Co-Authors: B V Ghule, Mahavir H. Ghante, A N Saoji, P G YeoleAbstract:Bottle gourd [(Lagenaria siceraria (Mol.) Stand.] fruit is ascribed with many therapeutic effects. The present study was undertaken to explore the antihyperlipidemic effect of four different extracts viz. petroleum ether, chloroform, alcoholic and aqueous extracts from bottle gourd in Triton-induced hyperlipidemic rats and their hypolipidemic effects in normocholesteremic rats. The study is comprised preliminary phytochemical screening of the extracts. Oral administration of the extracts, at doses of 200 and 400 mg/kg body weight in rats, dose-dependently inhibited the total cholesterol, triglycerides, low-density lipoproteins level, and significantly increased the high density lipoproteins level. However, petroleum ether extract did not show the significant effects. Both the chloroform and alcoholic extract exhibited more significant effects in lowering total cholesterol, triglycerides and low density lipoproteins along with increase in HDL as compared to the others. Preliminary phytochemical screening revealed the presence of flavonoids, sterols, cucurbitacin saponins, polyphenolics, proteins, and carbohydrates. The results obtained suggest marked antihyperlipidemic and hypolipidemic activity of the extracts.
Kendal D Hirschi - One of the best experts on this subject based on the ideXlab platform.
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ectopic expression of arabidopsis h pyrophosphatase avp1 enhances drought resistance in bottle gourd Lagenaria siceraria standl
Plant Cell Tissue and Organ Culture, 2014Co-Authors: Mehea Park, Jeungsul Han, Kendal D Hirschi, Yeulkyun Ahn, Jeongho Kim, Hyeeun Lee, Yoonah Jang, Roberto A Gaxiola, Sunghun ParkAbstract:Bottle gourd (Lagenaria siceraria Standl.) has been used as a source of rootstock for grafting watermelon to improve its fruit quality. We report here the development of a bottle gourd with resistance to drought by ectopic expression of the ArabidopsisAVP1 gene that encodes a vacuolar H+-pyrophosphatase. The drought resistance of AVP1-expressing and wild-type plants was assessed by growing plants under drought conditions. After 12 days of water deprivation, both AVP1-expressing and wild-type plants demonstrated reduced growth. After 10 days of re-watering, wild-type plants showed minimal growth while the AVP1-expressing plants resumed rapid growth. Further, AVP1-expressing plants displayed longer primary roots and more robust root systems than wild-type plants.
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agrobacterium mediated transformation of bottle gourd Lagenaria siceraria standl
Plant Cell Reports, 2005Co-Authors: Jungheon Han, Sunghun Park, Kendal D Hirschi, Chang Kil Kim, I G MokAbstract:We describe a procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 that carries the binary vector pCAMBIA3301 containing a glufosinate ammonium-resistance (bar) gene and the β-d-glucuronidase (GUS) reporter gene. The most effective bacterial infection was observed when cotyledon explants of 4-day-old seedlings were co-cultivated with Agrobacterium for 6–8 days on co-cultivation medium supplemented with 0.1–0.001 mg/l l-α-(2-aminoethoxyvinyl) glycine (AVG). The putatively transformed shoots directly emerged at the proximal end of cotyledon explants after 2–3 weeks of culturing on selection medium containing 2 mg/l dl-phosphinothricin. These shoots were rooted after 3 weeks of culturing on half-strength MS medium containing 0.1 mg/l indole acetic acid and 1 mg/l dl-phosphinothricin. Transgenic plants were obtained at frequencies of 1.9%. Stable integration and transmission of the transgenes in T1 generation plants were confirmed by a histochemical GUS assay, polymerase chain reaction and Southern blot analyses. Genetic segregation analysis of T1 progenies showed that transgenes were inherited in a Mendelian fashion. To our knowledge, this study is the first to show Agrobacterium-mediated transformation in bottle gourd.
Sunghun Park - One of the best experts on this subject based on the ideXlab platform.
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ectopic expression of arabidopsis h pyrophosphatase avp1 enhances drought resistance in bottle gourd Lagenaria siceraria standl
Plant Cell Tissue and Organ Culture, 2014Co-Authors: Mehea Park, Jeungsul Han, Kendal D Hirschi, Yeulkyun Ahn, Jeongho Kim, Hyeeun Lee, Yoonah Jang, Roberto A Gaxiola, Sunghun ParkAbstract:Bottle gourd (Lagenaria siceraria Standl.) has been used as a source of rootstock for grafting watermelon to improve its fruit quality. We report here the development of a bottle gourd with resistance to drought by ectopic expression of the ArabidopsisAVP1 gene that encodes a vacuolar H+-pyrophosphatase. The drought resistance of AVP1-expressing and wild-type plants was assessed by growing plants under drought conditions. After 12 days of water deprivation, both AVP1-expressing and wild-type plants demonstrated reduced growth. After 10 days of re-watering, wild-type plants showed minimal growth while the AVP1-expressing plants resumed rapid growth. Further, AVP1-expressing plants displayed longer primary roots and more robust root systems than wild-type plants.
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agrobacterium mediated transformation of bottle gourd Lagenaria siceraria standl
Plant Cell Reports, 2005Co-Authors: Jungheon Han, Sunghun Park, Kendal D Hirschi, Chang Kil Kim, I G MokAbstract:We describe a procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 that carries the binary vector pCAMBIA3301 containing a glufosinate ammonium-resistance (bar) gene and the β-d-glucuronidase (GUS) reporter gene. The most effective bacterial infection was observed when cotyledon explants of 4-day-old seedlings were co-cultivated with Agrobacterium for 6–8 days on co-cultivation medium supplemented with 0.1–0.001 mg/l l-α-(2-aminoethoxyvinyl) glycine (AVG). The putatively transformed shoots directly emerged at the proximal end of cotyledon explants after 2–3 weeks of culturing on selection medium containing 2 mg/l dl-phosphinothricin. These shoots were rooted after 3 weeks of culturing on half-strength MS medium containing 0.1 mg/l indole acetic acid and 1 mg/l dl-phosphinothricin. Transgenic plants were obtained at frequencies of 1.9%. Stable integration and transmission of the transgenes in T1 generation plants were confirmed by a histochemical GUS assay, polymerase chain reaction and Southern blot analyses. Genetic segregation analysis of T1 progenies showed that transgenes were inherited in a Mendelian fashion. To our knowledge, this study is the first to show Agrobacterium-mediated transformation in bottle gourd.
Honggun Park - One of the best experts on this subject based on the ideXlab platform.
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efficient plant regeneration from cotyledon explants of bottle gourd Lagenaria siceraria standl
Plant Cell Reports, 2004Co-Authors: D G Oh, Honggun ParkAbstract:Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO3 under a 16-h photoperiod. After 3–4 weeks of culture, 21.9–80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2–3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO3 were diploid.
