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James L. Kerwin - One of the best experts on this subject based on the ideXlab platform.
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oomycetes Lagenidium giganteum
Journal of The American Mosquito Control Association, 2007Co-Authors: James L. KerwinAbstract:ABSTRACT Lagenidium giganteum is a facultative parasite of mosquito larvae that initiates infection by production of biflagellate zoospores that selectively recognize and attach to larval cuticle. Following penetration of the cuticle, the parasite proliferates within the host, killing it within 24–60 h. Under optimum conditions the mycelia differentiate to produce asexual and/or sexual reproductive structures that produce zoospores within hours (asexual stage) to amplify the initial infection, or remain dormant for days, months or years (sexual stage), until conditions are conducive to mosquito breeding and spore germination. Recycling following a single application has been documented for up to 8–10 years. Environmental conditions that reduce or eliminate zoospore production, including temperature extremes (less than 16°C or greater than 32°C) and moderate levels of salinity and organic load, preclude use of the parasite for operational mosquito control. Three formulations of L. giganteum have been regis...
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Regulation of attachment, germination, and appressorium formation by zoospores of Lagenidium giganteum and related oomycetes by chitin, chitosan, and catecholamines
Protoplasma, 1997Co-Authors: E. E. Petersen, James L. Kerwin, M. J. Semon, J. M. BrowerAbstract:Lagenidium giganteum (Oomycetes: Lagenidiales), a facultative parasite of mosquito larvae, infects the larval stage of most species of mosquitoes and a very limited number of alternate hosts. Host infection by this and other members of Oomycetes is initiated by motile, laterally biflagellate zoospores. Chemical bases for the various degrees of host specificity exhibited by these parasites is not known, but presumably involves receptors on the zoospore surface recognizing compounds either secreted by or on the surface of their hosts. Surface topography had no detectable effect onL. giganteum encystment or appressorium formation. Scanning electron microscopy documented the detachment of flagella during zoospore encystment. Bulbous knobs at the basal end of the detached flagellum were interpreted as encysting zoospores dropping the axoneme and/or the basal body and associated structures to which flagella are attached. Multiple signals appear to be involved in the initial steps ofL. giganteum host invasion. Zoospores of this parasite did not encyst on powdered preparations of chitin or chitosan (deacetylated chitin). Upon dissolution of chitosan in dilute acid followed by drying these solutions to form thin, transparent films, zoospores readily encysted. The degree of reacetylation of these films and the spacing of acetylated and deacetylated residues had no significant effect on zoospore encystment. Zoospores of a strain ofLagenidium myophilum isolated from marine shrimp, that also infects mosquito larvae, encysted on chitosan films. No encystment of spores of the plant parasitePhytophthora capsici was observed on chitin or chitosan films. Simulation of cuticle sclerotization by incubating chitosan films with different catecholamines and tyrosinase significantly reduced zoospore encystment. Zoospores that encysted on chitosan films did not germinate in distilled water. Germination could be induced by adding microgram quantities of bovine serum albumin or proteins secreted by motile zoospores into the water, and to a lesser degree by some amino acids, but not by various cations. Zoospores encysted and germinated on the pupal stage of some mosquito species. Appressoria were occasionally formed, but most subsequently sent out another mycelial branch, apparently without attempting to pierce the pupal cuticle. Methylation of pupal exuviae with ethereal diazomethane or methanol/HCl significantly increased zoospore encystment. Modification of chitin by catecholamines, lipids and protein on the epicuticular larval surface all affected host invasion.
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Lagenidium giganteum in Culex tarsalis larvae: production of infective propagules.
Journal of invertebrate pathology, 1995Co-Authors: Jennifer L. Woodring, Harry K. Kaya, James L. KerwinAbstract:Zoospore and oospore production of the entomopathogenic fungus, Lagenidium giganteum, was quantified from Culex tarsalis larvae in rice irrigation water. For the first through fourth instars, respectively, the average zoospore production (+/- SE) was 521 +/- 106, 2458 +/- 365, 10,546 +/- 582, and 14,360 +/- 1076, and the average oospore production was 17 +/- 1.8, 33 +/- 3.5, 44 +/- 2.6, and 34 +/- 3.1. Zoosporogenesis varied with the water sample. The ionic composition of the water samples was significantly correlated with zoospore production. The correlations, however, changed as different subsets of data were analyzed, suggesting that other factors also affected zoosporogenesis. The extent to which a cadaver was colonized by other microorganisms had a clear effect on the resulting Lagenidium reproduction. The interval of peak zoosporogenesis occurred between 24 and 36 hr postmortem for the first instar and 12 to 24 hr postmortem for other instars. Measurable production was observed up to 96 hr postmortem from fourth instar larvae.
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Preliminary characterization of phospholipase A2 in Lagenidium giganteum
Experimental Mycology, 1994Co-Authors: Joanna K. Mackichan, Amy R. Tuininga, James L. KerwinAbstract:MacKichan, J. K., Tuininga, A. R., and Kerwin, J. L. 1994. Preliminary characterization of phospholipase A2 in Lagenidium giganteum. Experimental Mycology 18, 180-192. Phospholipase A2 (PLA2) hydrolyses the fatty acyl ester bond at the sn-2 position in glycerophospholipids. To better understand its regulatory roles, factors affecting PLA2 activity in Lagenidium giganteum were investigated: divalent ions; chelators: inhibitors; pH; and substrate concentration. PLA2 activity of L. giganteum whole cell homogenates was determined using 1-stearoyl-2-[1-14C]arachidonoyl phosphatidylcholine as substrate. The divalent cations Ca2+, Mg2+, and Mn2+ all enhanced PLA2 activity, while Co2+, Fe2+, and Zn2+ were either slightly inhibitory or without effect. High concentrations of EGTA enhanced activity, low concentrations of the chelators were slightly inhibitory, while high concentrations of EDTA had little effect. EGTA, which has a higher affinity for Ca2+ and Mn2+ than Mg2+, reduced hydrolysis less than a comparable concentration of EDTA. Two pH optima were found, at both acid (ca. 5.5) and alkaline (ca. 11.5) levels. Four classical inhibitors, nordihydroguaiaretic acid, ellagic acid, gossypol, and 4-bromophenacylbromide, reduced PLA2 activity by about 80% at 5 mM concentration, 50% with 1 mM inhibitor, and had no effect at 100 μM. The relatively high levels of these compounds needed to inhibit PLA2 hydrolysis may have been due to the presence of a cocktail of enzymes, some of which were not susceptible to inhibition. All inhibitors at 1 mM concentration in live cell cultures effectively shut down oosporogenesis, without adverse effects to the mycelia. PLA2 activity was highest in the late oospore stage of the life cycle, although the enzymes were probably not metabolically active in these stationary cultures. Cultures grown on cholesterol-supplemented defined media had significantly higher levels of PLA2 activity relative to cultures grown on sterol-free media. The enzyme was found to be associated primarily with microsomal membranes, but there was significant activity in cytosolic fractions. Separation of cell homogenates by column chromatography revealed that there were at least nine enzymes capable of cleaving fatty acids in the sn -2 position of phospholipids.
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ultrastructural and light microscopic localization of carbohydrates and peroxidase catalases in Lagenidium giganteum zoospores
Mycologia, 1993Co-Authors: Mary L. Berbee, James L. KerwinAbstract:Zoospores ofthe oomycete Lagenidium giganteum specifically recognize and then encyst upon mosquito larvae. As a step towards understanding the basis for this specific recognition, we examined the ultrastructure and cytochemistry of the zoospores. Zoospore shape and organellar arrangement are similar in E giganteum and other secondary-type oomycete zoospores. The ultrastructure of zoospores of L. giganteum differs from other Oomycetes mainly in details of vesicle morphology and mitochondrial arrangement. In our studies, we emphasized the outer surface of the zoospores where recognition molecules are likely to be present. Using silver methenamine and thiosemicarbazide-silver proteinate stain sequences, carbohydrate was found at the plasma membrane of zoospores. Ferritin- and fluorescein-labelled concanavalin A binding patterns indicated a-D-mannosyl and/or a-D-glucosyl residues are exposed on the outer surface of the plasma membrane of the zoospore and the wall of the cyst. Within the zoospores, the membranes of small peripheral vesicles, and the membranes and contents of golgi apparati and peripheral cisternae showed evidence of carbohydrate content with silver staining. We did not find clear evidence of carbohydrates among the contents of the large and small peripheral vesicles. Zoospores treated with diaminobenzidine to visualize peroxidases and catalases exhibited increased light and electron opacity in their mitochondria, as well as a fuzzy coat on the external surfaces
Leonel Mendoza - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae
Mycologia, 2019Co-Authors: John W. Taylor, Raquel Vilela, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderate...
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Phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae.
Mycologia, 2019Co-Authors: Raquel Vilela, John W. Taylor, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderately at 30 C, and not at all at 37 C and developed submerged, white colonies with few short, hyaline, aerial hyphae. Cultures displayed phenotypic plasticity, with broad, hyaline hyphae strongly constricted at septa that developed oval, spherical, or amorphous segments. These developed into sporangia producing one or two exit tubes, from which evanescent gelatinous vesicles containing zoospores developed. Three isolates developed oogonia consistent with features previously described for L. giganteum. Phylogenetic analysis of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS = ITS) and cytochrome c oxidase subunit II (COXII) sequences of L. giganteum consistently grouped into eight clusters. Four of the investigated isolates grouped with sequences of an unnamed Lagenidium species infecting nematodes. Based on phenotypic and phylogenetic data, we describe the latter isolates as L. juracyae, sp. nov. In addition, we also investigated a species of ParaLagenidium from a dog with lagenidiosis and describe it as new, ParaLagenidium ajellopsis, sp. nov.
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Description of three novel Lagenidium (Oomycota) species causing infection in mammals.
Revista iberoamericana de micologia, 2016Co-Authors: Leonel Mendoza, John W. Taylor, Edward D. Walker, Raquel VilelaAbstract:Abstract Background Recent molecular phylogenetic analysis of Lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was Lagenidium giganteum , but three others were novel. Aims Due to the recent increase in L. giganteum infections from mammals, we studied 21 Lagenidium strains isolated from dogs and a human available in our collection. Methods Molecular phylogenetic studies and phenotypic characteristics were used to characterize the strains. Results We report the finding of three novel species, herein designated as Lagenidium ajelloi , sp. nov., Lagenidium albertoi sp. nov, and Lagenidium vilelae sp. nov. Their morphological and growth features are also presented. Conclusions Our study revealed the presence of three novel Lagenidium species infecting mammals.
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A biochemical screening approach to putatively differentiate mammalian pathogenic Oomycota species in the clinical laboratory.
Journal of medical microbiology, 2015Co-Authors: Raquel Vilela, Poorna Viswanathan, Leonel MendozaAbstract:The report of four novel mammalian pathogenic species of the genus Lagenidium prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens Pythium insidiosum and Lagenidium spp. We investigated the reaction of 23 Lagenidium and eight Pythium species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of Pythium spp. all hydrolysed sucrose, but Lagenidium species and the fungal strains did not. In addition, both Pythium spp. and Lagenidium spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive Lagenidium giganteum and Lagenidium humanum were urease-negative, but the mammalian Lagenidium spp. and Pythium spp. hydrolysed urea within 24 h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.
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Lagenidium giganteum Pathogenicity in Mammals
Emerging infectious diseases, 2015Co-Authors: Raquel Vilela, John W. Taylor, Edward D. Walker, Leonel MendozaAbstract:Infections of mammals by species in the phylum Oomycota taxonomically and molecularly similar to known Lagenidium giganteum strains have increased. During 2013–2014, we conducted a phylogenetic study of 21 mammalian Lagenidium isolates; we found that 11 cannot be differentiated from L. giganteum strains that the US Environmental Protection Agency approved for biological control of mosquitoes; these strains were later unregistered and are no longer available. L. giganteum strains pathogenic to mammals formed a strongly supported clade with the biological control isolates, and both types experimentally infected mosquito larvae. However, the strains from mammals grew well at 25°C and 37°C, whereas the biological control strains developed normally at 25°C but poorly at higher temperatures. The emergence of heattolerant strains of L. giganteum pathogenic to lower animals and humans is of environmental and public health concern.
Soam Prakash - One of the best experts on this subject based on the ideXlab platform.
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Efficacy of the Trichophyton ajelloi and Lagenidium giganteum metabolites against mosquitoes after flash chromatography
Parasitology Research, 2012Co-Authors: Gavendra Singh, Soam PrakashAbstract:Entomopathogens are significant natural enemies for mosquitoes. We have investigated the adulticidal efficacies of metabolites of Trichophyton ajelloi and Lagenidium giganteum against Culex quinquefasciatus and Aedes aegypti simultaneously. The T. ajelloi was grown on Sabouraud’s dextrose broth medium at 25 ± 2°C and relative humidity at 75 ± 5% for 15 days. L . giganteum was grown in peptone yeast extract glucose broth at 25 ± 2°C and relative humidity 75 ± 5% for 15 days. The filtrations of metabolites have been made by using Whatman-1 filter paper then with the flash chromatograph. The bioassays were conducted as per the World Health Organization’s methods and protocols (2006). In this significant investigations, the metabolites of T . ajelloi have been found highly susceptible against A . aegypti with LC_99-7.24 ml after an exposure time of 24 h with a comparison, the LC_99-66 ml was observed against C . quinquefasciatus after exposure of 24 h. Moreover, the L . giganteum metabolites have shown higher pathogenicity against C . quinquefasciatus with LC_99-11.3 ml and A . aegypti with LC_99-15.49 ml. Although, the efficacy in adults could be achieved with higher concentration can be significant also. Their adulticidal activities in different climatic zones are plausible with metabolites which have better LT values of T . ajelloi .
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Studies on Fungal Cultural Filtrates against Adult Culex quinquefasciatus (Diptera: Culicidae) a Vector of Filariasis
Journal of parasitology research, 2011Co-Authors: Gavendra Singh, Soam PrakashAbstract:Entomopathogenic fungi have significant potential to control mosquito population. The culture filtrates of Fusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloi, and Culicinomyces clavisporus were evaluated against adults of Cx. quinquefasciatus. The culture filtrates were obtained by filtering the broth through Whatman-1 filter paper. These culture filtrates of C. clavisporus have been found significantly pathogenic with LC50-2.5, LC90-7.24, and LC99-8.7 ML, respectively, after exposure of 24 h. However, the culture filtrates when were combined, in ratios 1 : 1 : 1 of Fusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloi the mortalities were significantly increased. The LC50-3.71, LC90-8.12, and LC99-11.48 were significantly recorded after exposure of 10 hrs. Similarly, the culture filtrates of T. ajelloi, Culicinomyces clavisporus, and L. giganteum have been combined in ratios 1 : 1 : 1. Similarly the LC50-1.94, LC90-4, and LC99-6.16 ML Were recorded after exposure of 10 hrs. The results of present study show promise for the use of selected fungal metabolites for control of Cx. quinquefasciatus in the Laboratory.
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doi:10.1155/2011/147373 Research Article Studies on Fungal Cultural Filtrates against Adult Culex quinquefasciatus (Diptera: Culicidae) a Vector of Filariasis
2011Co-Authors: Gavendra Singh, Soam PrakashAbstract:License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Entomopathogenic fungi have significant potential to control mosquito population. The culture filtrates of Fusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloi, and Culicinomyces clavisporus were evaluated against adults of Cx. quinquefasciatus. The culture filtrates were obtained by filtering the broth through Whatman-1 filter paper. These culture filtrates of C. clavisporus have been found significantly pathogenic with LC50-2.5, LC90-7.24, and LC99-8.7 ML, respectively, after exposure of 24 h. However, the culture filtrates when were combined, in ratios 1: 1: 1 of Fusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloi the mortalities were significantly increased. The LC50-3.71, LC90-8.12, and LC99-11.48 were significantly recorded after exposure of 10 hrs. Similarly, the culture filtrates of T. ajelloi, Culicinomyces clavisporus, and L. giganteum have been combined in ratios 1:1:1. Similarly the LC50-1.94, LC90-4, and LC99-6.16 ML Were recorded after exposure of 10 hrs. The results of present study show promise for the use of selected fungal metabolites for control of Cx. quinquefasciatus in the Laboratory. 1
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Efficacy of Lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms.
Parasitology research, 2007Co-Authors: Neetu Vyas, Kamal Dua, Soam PrakashAbstract:Lagenidium giganteum is a water mold and an effective mosquito control agent with limited use due to poor survival and contamination during storage. Invert extracellular metabolites of L. giganteum is easy to produce, long shelf life, and a potential candidate in tropical climates. This fungus was grown in PYG broth in the laboratory at 25 ± 2°C, and relative humidity was maintained at 75 ± 5% for 15 ± 2 days. Filtration process of metabolites was done using Whatman filter paper, column chromatograph, and range syringe filters techniques. Then 5-ml fractions were collected and used to assay larvicidal efficacies. Larvicidal efficacies were performed against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti at five different concentrations, viz. 1.68, 1.99, 2.17, 2.30, and 2.40 ppm. And also, filtrates were assessed against four species of nontarget organisms named Daphnia pulex, Cyclopes, Lymnea auriculeta, and tadpoles of Rana tigrina with different concentrations. The mortality values were subjected by the Probit analysis. The complete mortalities that resulted from applying filtrates dosage on all instars of mosquitoes persisted for a period of 24, 48, and 72 h, respectively. The efficacies in killing instar of three important vectors and safer for nontarget organisms with good biological stability of extracellular metabolites make this a promising alternative to mycelium and conidial-based larvicides. It could be regarded as fungal-based natural larvicide for the use of vector control.
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Laboratory efficacy of metabolites of Lagenidium giganteum (Couch) on Anopheles stephensi (Liston) after filterations by Column Chromatography.
The Journal of communicable diseases, 2006Co-Authors: Neetu Vyas, Kamal Dua, Soam PrakashAbstract:This study was conducted in laboratory to evaluate the efficacy of filtered extracellular metabolites of Lagenidium giganteum against all the four instars of An. stephensi larvae. Fungal colonies have been cultured in PYG broth and after 15 days of culturing the fungus, metabolites have been filtered twice by whatman filter paper. These m etabolites were again filtered by colum n chromatography and by rang syringe filters. Filtered metabolites were then used against all instars of An. stephensi larvae. The bioassays were conducted at five significantly different concentrations (1.68, 1.99, 2.17, 2.30, 2.40 ppm). The results suggest significant mortality on first three instar larvae and very low on fourth instar larvae.
Raquel Vilela - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae.
Mycologia, 2019Co-Authors: Raquel Vilela, John W. Taylor, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderately at 30 C, and not at all at 37 C and developed submerged, white colonies with few short, hyaline, aerial hyphae. Cultures displayed phenotypic plasticity, with broad, hyaline hyphae strongly constricted at septa that developed oval, spherical, or amorphous segments. These developed into sporangia producing one or two exit tubes, from which evanescent gelatinous vesicles containing zoospores developed. Three isolates developed oogonia consistent with features previously described for L. giganteum. Phylogenetic analysis of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS = ITS) and cytochrome c oxidase subunit II (COXII) sequences of L. giganteum consistently grouped into eight clusters. Four of the investigated isolates grouped with sequences of an unnamed Lagenidium species infecting nematodes. Based on phenotypic and phylogenetic data, we describe the latter isolates as L. juracyae, sp. nov. In addition, we also investigated a species of ParaLagenidium from a dog with lagenidiosis and describe it as new, ParaLagenidium ajellopsis, sp. nov.
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phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae
Mycologia, 2019Co-Authors: John W. Taylor, Raquel Vilela, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderate...
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Description of three novel Lagenidium (Oomycota) species causing infection in mammals.
Revista iberoamericana de micologia, 2016Co-Authors: Leonel Mendoza, John W. Taylor, Edward D. Walker, Raquel VilelaAbstract:Abstract Background Recent molecular phylogenetic analysis of Lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was Lagenidium giganteum , but three others were novel. Aims Due to the recent increase in L. giganteum infections from mammals, we studied 21 Lagenidium strains isolated from dogs and a human available in our collection. Methods Molecular phylogenetic studies and phenotypic characteristics were used to characterize the strains. Results We report the finding of three novel species, herein designated as Lagenidium ajelloi , sp. nov., Lagenidium albertoi sp. nov, and Lagenidium vilelae sp. nov. Their morphological and growth features are also presented. Conclusions Our study revealed the presence of three novel Lagenidium species infecting mammals.
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A biochemical screening approach to putatively differentiate mammalian pathogenic Oomycota species in the clinical laboratory.
Journal of medical microbiology, 2015Co-Authors: Raquel Vilela, Poorna Viswanathan, Leonel MendozaAbstract:The report of four novel mammalian pathogenic species of the genus Lagenidium prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens Pythium insidiosum and Lagenidium spp. We investigated the reaction of 23 Lagenidium and eight Pythium species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of Pythium spp. all hydrolysed sucrose, but Lagenidium species and the fungal strains did not. In addition, both Pythium spp. and Lagenidium spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive Lagenidium giganteum and Lagenidium humanum were urease-negative, but the mammalian Lagenidium spp. and Pythium spp. hydrolysed urea within 24 h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.
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Lagenidium giganteum Pathogenicity in Mammals
Emerging infectious diseases, 2015Co-Authors: Raquel Vilela, John W. Taylor, Edward D. Walker, Leonel MendozaAbstract:Infections of mammals by species in the phylum Oomycota taxonomically and molecularly similar to known Lagenidium giganteum strains have increased. During 2013–2014, we conducted a phylogenetic study of 21 mammalian Lagenidium isolates; we found that 11 cannot be differentiated from L. giganteum strains that the US Environmental Protection Agency approved for biological control of mosquitoes; these strains were later unregistered and are no longer available. L. giganteum strains pathogenic to mammals formed a strongly supported clade with the biological control isolates, and both types experimentally infected mosquito larvae. However, the strains from mammals grew well at 25°C and 37°C, whereas the biological control strains developed normally at 25°C but poorly at higher temperatures. The emergence of heattolerant strains of L. giganteum pathogenic to lower animals and humans is of environmental and public health concern.
Ariya Chindamporn - One of the best experts on this subject based on the ideXlab platform.
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Lagenidium sp. Ocular Infection Mimicking Ocular Pythiosis
Journal of Clinical Microbiology, 2013Co-Authors: Usanee Reinprayoon, Nitipong Permpalung, Rongpong Plongla, Ngamjit Kasetsuwan, Leonel Mendoza, Ariya ChindampornAbstract:This is a report of a Lagenidium sp. in a Thai patient who was diagnosed with severe keratitis that was unresponsive to antibacterial and antifungal drugs. Examination of a corneal biopsy specimen confirmed the presence of aseptate hyphae. The internal transcribed spacer DNA sequence of the strain isolated showed 97% identity with Lagenidium giganteum and other Lagenidium species.
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phylogenetic analysis of pythium insidiosum thai strains using cytochrome oxidase ii cox ii dna coding sequences and internal transcribed spacer regions its
Medical Mycology, 2011Co-Authors: Patcharee Kammarnjesadakul, Leonel Mendoza, Nongnuch Vanittanakom, Tanapat Palaga, Kallaya Sritunyalucksana, Theerapong Krajaejun, Songsak Tongchusak, Jessada Denduangboripant, Ariya ChindampornAbstract:To investigate the phylogenetic relationship among Pythium insidiosum isolates in Thailand, we investigated the genomic DNA of 31 P. insidiosum strains isolated from humans and environmental sources from Thailand, and two from North and Central America. We used PCR to amplify the partial COX II DNA coding sequences and the ITS regions of these isolates. The nucleotide sequences of both amplicons were analyzed by the Bioedit program. Phylogenetic analysis using genetic distance method with Neighbor Joining (NJ) approach was performed using the MEGA4 software. Additional sequences of three other Pythium species, Phytophthora sojae and Lagenidium giganteum were employed as outgroups. The sizes of the COX II amplicons varied from 558–564 bp, whereas the ITS products varied from approximately 871–898 bp. Corrected sequence divergences with Kimura 2-parameter model calculated for the COX II and the ITS DNA sequences ranged between 0.0000–0.0608 and 0.0000–0.2832, respectively. Phylogenetic analysis using both t...
