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Leonel Mendoza - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae
Mycologia, 2019Co-Authors: John W. Taylor, Raquel Vilela, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderate...
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Phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae.
Mycologia, 2019Co-Authors: Raquel Vilela, John W. Taylor, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderately at 30 C, and not at all at 37 C and developed submerged, white colonies with few short, hyaline, aerial hyphae. Cultures displayed phenotypic plasticity, with broad, hyaline hyphae strongly constricted at septa that developed oval, spherical, or amorphous segments. These developed into sporangia producing one or two exit tubes, from which evanescent gelatinous vesicles containing zoospores developed. Three isolates developed oogonia consistent with features previously described for L. giganteum. Phylogenetic analysis of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS = ITS) and cytochrome c oxidase subunit II (COXII) sequences of L. giganteum consistently grouped into eight clusters. Four of the investigated isolates grouped with sequences of an unnamed Lagenidium species infecting nematodes. Based on phenotypic and phylogenetic data, we describe the latter isolates as L. juracyae, sp. nov. In addition, we also investigated a species of ParaLagenidium from a dog with lagenidiosis and describe it as new, ParaLagenidium ajellopsis, sp. nov.
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Description of three novel Lagenidium (Oomycota) species causing infection in mammals.
Revista iberoamericana de micologia, 2016Co-Authors: Leonel Mendoza, John W. Taylor, Edward D. Walker, Raquel VilelaAbstract:Abstract Background Recent molecular phylogenetic analysis of Lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was Lagenidium giganteum , but three others were novel. Aims Due to the recent increase in L. giganteum infections from mammals, we studied 21 Lagenidium strains isolated from dogs and a human available in our collection. Methods Molecular phylogenetic studies and phenotypic characteristics were used to characterize the strains. Results We report the finding of three novel species, herein designated as Lagenidium ajelloi , sp. nov., Lagenidium albertoi sp. nov, and Lagenidium vilelae sp. nov. Their morphological and growth features are also presented. Conclusions Our study revealed the presence of three novel Lagenidium species infecting mammals.
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A biochemical screening approach to putatively differentiate mammalian pathogenic Oomycota species in the clinical laboratory.
Journal of medical microbiology, 2015Co-Authors: Raquel Vilela, Poorna Viswanathan, Leonel MendozaAbstract:The report of four novel mammalian pathogenic species of the genus Lagenidium prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens Pythium insidiosum and Lagenidium spp. We investigated the reaction of 23 Lagenidium and eight Pythium species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of Pythium spp. all hydrolysed sucrose, but Lagenidium species and the fungal strains did not. In addition, both Pythium spp. and Lagenidium spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive Lagenidium giganteum and Lagenidium humanum were urease-negative, but the mammalian Lagenidium spp. and Pythium spp. hydrolysed urea within 24 h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.
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Lacazia, Lagenidium, Pythium, and Rhinosporidium
Manual of Clinical Microbiology, 2015Co-Authors: Raquel Vilela, Leonel MendozaAbstract:In the past 100 years the microbial pathogens described in this chapter have been classified either as fungal and/or para-fungal protistan pathogens. Based on their apparent epidemiological connection with water, they were at one point also placed in a new category of hydrophilic infectious agents. However, based on taxonomic and other morphological characteristics, these three anomalous species were not well understood. This frustrating situation fueled a strong controversy that has only recently been solved with the advent of molecular methodologies. Despite the recent finding that both Pythium insidiosum and Rhinosporidium seeberi are protistan pathogens, they are still studied by medical mycologists, continuing a historical tradition. More recently, the finding of an oomycete in the genus Lagenidium affecting mammalian hosts alerted the medical community to the presence of a novel pathogen phenotypically similar to the fungi and indistinguishable from the clinical and pathological features displayed by P. insidiosum during infection. Based on rDNA phylogenetic analysis, the evolutionary location of the microbial pathogens discussed in this chapter is illustrated.
Raquel Vilela - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae
Mycologia, 2019Co-Authors: John W. Taylor, Raquel Vilela, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderate...
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Phylogenetic and physiological traits of oomycetes originally identified as Lagenidium giganteum from fly and mosquito larvae.
Mycologia, 2019Co-Authors: Raquel Vilela, John W. Taylor, Richard A Humber, Leonel MendozaAbstract:We studied phylogenetic and taxonomic features of isolates identified as Lagenidium giganteum recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderately at 30 C, and not at all at 37 C and developed submerged, white colonies with few short, hyaline, aerial hyphae. Cultures displayed phenotypic plasticity, with broad, hyaline hyphae strongly constricted at septa that developed oval, spherical, or amorphous segments. These developed into sporangia producing one or two exit tubes, from which evanescent gelatinous vesicles containing zoospores developed. Three isolates developed oogonia consistent with features previously described for L. giganteum. Phylogenetic analysis of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS = ITS) and cytochrome c oxidase subunit II (COXII) sequences of L. giganteum consistently grouped into eight clusters. Four of the investigated isolates grouped with sequences of an unnamed Lagenidium species infecting nematodes. Based on phenotypic and phylogenetic data, we describe the latter isolates as L. juracyae, sp. nov. In addition, we also investigated a species of ParaLagenidium from a dog with lagenidiosis and describe it as new, ParaLagenidium ajellopsis, sp. nov.
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Description of three novel Lagenidium (Oomycota) species causing infection in mammals.
Revista iberoamericana de micologia, 2016Co-Authors: Leonel Mendoza, John W. Taylor, Edward D. Walker, Raquel VilelaAbstract:Abstract Background Recent molecular phylogenetic analysis of Lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was Lagenidium giganteum , but three others were novel. Aims Due to the recent increase in L. giganteum infections from mammals, we studied 21 Lagenidium strains isolated from dogs and a human available in our collection. Methods Molecular phylogenetic studies and phenotypic characteristics were used to characterize the strains. Results We report the finding of three novel species, herein designated as Lagenidium ajelloi , sp. nov., Lagenidium albertoi sp. nov, and Lagenidium vilelae sp. nov. Their morphological and growth features are also presented. Conclusions Our study revealed the presence of three novel Lagenidium species infecting mammals.
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A biochemical screening approach to putatively differentiate mammalian pathogenic Oomycota species in the clinical laboratory.
Journal of medical microbiology, 2015Co-Authors: Raquel Vilela, Poorna Viswanathan, Leonel MendozaAbstract:The report of four novel mammalian pathogenic species of the genus Lagenidium prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens Pythium insidiosum and Lagenidium spp. We investigated the reaction of 23 Lagenidium and eight Pythium species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of Pythium spp. all hydrolysed sucrose, but Lagenidium species and the fungal strains did not. In addition, both Pythium spp. and Lagenidium spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive Lagenidium giganteum and Lagenidium humanum were urease-negative, but the mammalian Lagenidium spp. and Pythium spp. hydrolysed urea within 24 h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.
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Lacazia, Lagenidium, Pythium, and Rhinosporidium
Manual of Clinical Microbiology, 2015Co-Authors: Raquel Vilela, Leonel MendozaAbstract:In the past 100 years the microbial pathogens described in this chapter have been classified either as fungal and/or para-fungal protistan pathogens. Based on their apparent epidemiological connection with water, they were at one point also placed in a new category of hydrophilic infectious agents. However, based on taxonomic and other morphological characteristics, these three anomalous species were not well understood. This frustrating situation fueled a strong controversy that has only recently been solved with the advent of molecular methodologies. Despite the recent finding that both Pythium insidiosum and Rhinosporidium seeberi are protistan pathogens, they are still studied by medical mycologists, continuing a historical tradition. More recently, the finding of an oomycete in the genus Lagenidium affecting mammalian hosts alerted the medical community to the presence of a novel pathogen phenotypically similar to the fungi and indistinguishable from the clinical and pathological features displayed by P. insidiosum during infection. Based on rDNA phylogenetic analysis, the evolutionary location of the microbial pathogens discussed in this chapter is illustrated.
Charles Santhanaraju Vairappan - One of the best experts on this subject based on the ideXlab platform.
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12Epi -9deacetoxyxenicin, new cytotoxic diterpenoid from a Bornean soft coral Xenia sp
'Informa UK Limited', 2019Co-Authors: Phan, Chin Soon, Takashi Kamada, Takahiro Ishii, Toshiyuki Hamada, Charles Santhanaraju VairappanAbstract:One new compound, 12-epi-9-deacetoxyxenicin (1) along with a hydroperoxide product, 12-epi-9-deacetoxy-8-hydroperoxyxenicin (2) and two known sesquiterpenoids (3–4) were isolated from a population of Bornean soft coral Xenia sp. The structures of these secondary metabolites were elucidated based on their spectroscopic data. Compounds 1 and 2 showed cytotoxic activity against ATL cell line, S1T. In addition, compound 3 exhibited hyphal inhibition of Lagenidium thermophilum
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Nangallenes A and B, halogenated nonterpenoid C15-acetogenins from the Bornean red alga Laurencia nangii
'Informa UK Limited', 2019Co-Authors: Takashi Kamada, Phan, Chin Soon, Charles Santhanaraju VairappanAbstract:Two new halogenated nonterpenoids C15-acetogenins, nangallenes A-B (1–2), together with two known halogenated compounds itomanallene A (3) and 2,10-dibromo -3-chloro-α- chamigrene (4), were isolated and identified from the organic extract of the marine red alga Laurencia nangii Masuda collected from the coastal waters in Semporna, Borneo. Their structures were established by means of spectroscopic analysis including IR, high-resolution electrospray ionization mass spectrometry (HRESI-MS), and 1D and 2D NMR techniques. All these metabolites were submitted for the antifungal assay against four species of selected marine fungi. Compounds 1–4 showed potent activity against Haliphthoros sabahensis and Lagenidium thermophilum
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In Vitro Inhibitory Effects of Two Bornean Medicinal Wild Gingers Against Pathogenic Lagenidium Thermophilum Infected Mud Crab Scylla Tranquebarica
'The Society for Antibacterial and Antifungal Agents Japan', 2018Co-Authors: Kishio Hatai, Chin-soon Phan, Takashi Kamada, Lau, Lik Ming, Julius Kulip, Charles Santhanaraju VairappanAbstract:The antifungal activity of two Bornean medicinal wild gingers Plagiostachys megacarpa and Zingiber phillippsiae were examined against Lagenidium thermophilum. The most active extract was P. megacarpa at concentration of 320 µg/mL inhibiting both hyphal growth and zoospore production of L. thermophilum in 24 h. Toxicity tests were conducted using mud crab (Scylla tranquebarica) larva. Bath treatment of P. megacarpa at concentrations of 320 and 640 µg/mL for 24 h were highly effective against hyphae and zoospores of the strain and it is non-toxic to mud crab larva. Therefore, crude extracts P. megacarpa may be used as alternative treatment for marine Oomycete infection of mud crab
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12-Epi-9-deacetoxyxenicin, new cytotoxic diterpenoid from a Bornean soft coral, Xenia sp.
2017Co-Authors: Chin-soon Phan, Takashi Kamada, Takahiro Ishii, Toshiyuki Hamada, Charles Santhanaraju VairappanAbstract:One new compound, 12-epi-9-deacetoxyxenicin (1) along with a hydroperoxide product, 12-epi-9-deacetoxy-8-hydroperoxyxenicin (2) and two known sesquiterpenoids (3–4) were isolated from a population of Bornean soft coral Xenia sp. The structures of these secondary metabolites were elucidated based on their spectroscopic data. Compounds 1 and 2 showed cytotoxic activity against ATL cell line, S1T. In addition, compound 3 exhibited hyphal inhibition of Lagenidium thermophilum.
Kishio Hatai - One of the best experts on this subject based on the ideXlab platform.
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In Vitro Inhibitory Effects of Two Bornean Medicinal Wild Gingers Against Pathogenic Lagenidium Thermophilum Infected Mud Crab Scylla Tranquebarica
'The Society for Antibacterial and Antifungal Agents Japan', 2018Co-Authors: Kishio Hatai, Chin-soon Phan, Takashi Kamada, Lau, Lik Ming, Julius Kulip, Charles Santhanaraju VairappanAbstract:The antifungal activity of two Bornean medicinal wild gingers Plagiostachys megacarpa and Zingiber phillippsiae were examined against Lagenidium thermophilum. The most active extract was P. megacarpa at concentration of 320 µg/mL inhibiting both hyphal growth and zoospore production of L. thermophilum in 24 h. Toxicity tests were conducted using mud crab (Scylla tranquebarica) larva. Bath treatment of P. megacarpa at concentrations of 320 and 640 µg/mL for 24 h were highly effective against hyphae and zoospores of the strain and it is non-toxic to mud crab larva. Therefore, crude extracts P. megacarpa may be used as alternative treatment for marine Oomycete infection of mud crab
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First report of Lagenidium thermophilum isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia.
Bulletin of The European Association of Fish Pathologists, 2016Co-Authors: Y. N Lee, Kishio Hatai, Osamu KurataAbstract:In April 2014, marine Oomycetes were first isolated from mud crab Scylla tranquebarica eggs and larvae at the University Malaysia Sabah shrimp hatchery. A fungus was isolated from infected eggs and larvae using PYGS agar. It was thought that the same fungus infected both eggs and larvae; therefore, strain IPMB 1401 was randomly selected for further characterization in this study. The isolated fungus produced a discharge tube from the mycelium, and a vesicle was formed at the tip. The zoospores swam away after the vesicle separated from the discharge tube. The strain IPMB 1401 was classified as a Lagenidium sp., closely related to L. thermophilum based on the mode of zoospore release. The differences between the strains IPMB 1401 and pathogenic Lagenidium spp. isolated from marine crustaceans were compared in nucleotide sequence of ITS 1 region. As a result, the IPMB 1401 showed high similarity of 99-100% and belonged to the same cluster with L. thermophilum. Therefore, the strain IPMB 1401 was identified as L. thermophilum. This is the first report of Lagenidium infection in Malaysia.
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Lagenidium thermophilum isolated from eggs and larvae of black tiger shrimp penaeus monodon in thailand
Fish Pathology, 2006Co-Authors: Yasunori Muraosa, Ongard Lawhavinit, Kishio HataiAbstract:ABSTRACT —A fungal infection was found in eggs and larvae of black tiger shrimp Penaeusmonodon at a hatchery in Chachensao Province, Thailand in August 2000. Fungi were isolatedfrom eggs and larvae with fungal infection, and studied on the morphological and biological charac-teristics. When it was transferred from PYGS broth to artificial seawater, discharge tubes devel-oped from the mycelia, and a vesicle for zoospore formation was produced at the top of eachdischarge tube. The characteristic feature of an asexual reproduction of the fungus was thatzoospores swam away in seawater after the vesicle separated from the discharge tube. Based onthese morphological characteristics, the fungus was identified as Lagenidium thermophilum.Some biological characteristics of the selected isolate NJM 0031 were compared with the otherspecies in the genus Lagenidium isolated from some crustaceans. As a result, the isolate NJM0031 showed similar characteristics to those of L. thermophilum ATCC 200318 isolated from man-grove crab Scylla serrata. The isolate was demonstrated to be pathogenic to larvae of black tigershrimp by artificial infection. This is the first report of L. thermophilum infection in black tigershrimp in Thailand.
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Identification of lower fungi isolated from larvae of mangrove crab,Scylla serrata, in Indonesia
Mycoscience, 2000Co-Authors: Kishio Hatai, Des Roza, Takane NakayamaAbstract:Since 1992, seed production of mangrove crab, Scylla serrata , has been attempted at the Gondol Research Station for Coastal Fisheries, Bali, Indonesia. During the production process, almost all of the larvae have died due to fungal infection. Fungi isolated from the larvae with fungal infection were classified into three species in the order Lagenidiales: Lagenidium callinectes, Haliphthoros milfordensis and Halocrusticida baliensis sp. nov. based on detailed morphological characteristics. The effects of temperature, pH and mineral content of the water on their growth were also examined.
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Pathogenicity of fungi isolated from the larvae of the mangrove crab,Scylla serrata, in Indonesia
Mycoscience, 1999Co-Authors: Des Roza, Kishio HataiAbstract:Heavy mortality reaching almost 100% occurred in the larvae of the mangrove crab, Scylla serrata , from July to December 1997 at the hatchery of Gondol Research Station for Coastal Fisheries in Bali, Indonesia. Mortality was observed in larvae after hatching from the eggs. The affected larvae were whitish and filled with numerous aseptate hyphae. Three fungi belonging to the order Lagenidiales, Lagenidium callinectes, Haliphthoros milfordensis , and Halocrusticida sp., were isolated from the infected larvae. Pathogenicity tests of the infected fungi against the larvae of mangrove crab demonstrated that all isolates were pathogenic.
Eberhard Schnepf - One of the best experts on this subject based on the ideXlab platform.
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Infection of Glaucocystis nostochinearum (Glaucophyta) by Lagenidium sp. (Oomycota) and its hyperparasite Pythiella sp. (Oomycota)
Hydrobiologia, 2002Co-Authors: Stefanie F. Kühn, Eberhard SchnepfAbstract:The glaucophyte Glaucocystis nostochinearum has to our knowledge been observed to be infected by a parasite for the first time. It was found in samples taken from the northernmost freshwater pond in Germany (on the island of Sylt). The fungal parasite was identified as the oomycete Lagenidium sp. which itself was parasitised by another oomycete, Pythiella sp.
