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Zhili Suo - One of the best experts on this subject based on the ideXlab platform.
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chloroplast phylogenomics and divergence times of Lagerstroemia lythraceae
BMC Genomics, 2021Co-Authors: Wenpan Dong, Yanlei Liu, Jipu Shi, Zhili SuoAbstract:Crape myrtles, belonging to the genus Lagerstroemia L., have beautiful paniculate inflorescences and are cultivated as important ornamental tree species for landscaping and gardening. However, the phylogenetic relationships within Lagerstroemia have remained unresolved likely caused by limited sampling and the insufficient number of informative sites used in previous studies. In this study, we sequenced 20 Lagerstroemia chloroplast genomes and combined with 15 existing chloroplast genomes from the genus to investigate the phylogenetic relationships and divergence times within Lagerstroemia. The phylogenetic results indicated that this genus is a monophyletic group containing four clades. Our dating analysis suggested that Lagerstroemia originated in the late Paleocene (~ 60 Ma) and started to diversify in the middle Miocene. The diversification of most species occurred during the Pleistocene. Four variable loci, trnD-trnY-trnE, rrn16-trnI, ndhF-rpl32-trnL and ycf1, were discovered in the Lagerstroemia chloroplast genomes. The chloroplast genome information was successfully utilized for molecular characterization of diverse crape myrtle samples. Our results are valuable for the global genetic diversity assessment, conservation and utilization of Lagerstroemia.
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Chloroplast phylogenomics and divergence times of Lagerstroemia (Lythraceae)
'Springer Science and Business Media LLC', 2021Co-Authors: Wenpan Dong, Yanlei Liu, Jipu Shi, Zhili SuoAbstract:Abstract Background Crape myrtles, belonging to the genus Lagerstroemia L., have beautiful paniculate inflorescences and are cultivated as important ornamental tree species for landscaping and gardening. However, the phylogenetic relationships within Lagerstroemia have remained unresolved likely caused by limited sampling and the insufficient number of informative sites used in previous studies. Results In this study, we sequenced 20 Lagerstroemia chloroplast genomes and combined with 15 existing chloroplast genomes from the genus to investigate the phylogenetic relationships and divergence times within Lagerstroemia. The phylogenetic results indicated that this genus is a monophyletic group containing four clades. Our dating analysis suggested that Lagerstroemia originated in the late Paleocene (~ 60 Ma) and started to diversify in the middle Miocene. The diversification of most species occurred during the Pleistocene. Four variable loci, trnD-trnY-trnE, rrn16-trnI, ndhF-rpl32-trnL and ycf1, were discovered in the Lagerstroemia chloroplast genomes. Conclusions The chloroplast genome information was successfully utilized for molecular characterization of diverse crape myrtle samples. Our results are valuable for the global genetic diversity assessment, conservation and utilization of Lagerstroemia
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comparative analysis of six Lagerstroemia complete chloroplast genomes
Frontiers in Plant Science, 2017Co-Authors: Wenpan Dong, Jipu Shi, Xiaoman Xie, Xiaobai Jin, Zhili SuoAbstract:Crape myrtles are economically important tree species of the genus Lagerstroemia L. (Lythraceae), with a distribution from tropical to northern temperate zones. They are positioned phylogenetically to a large subclade of rosids (in the eudicots) which contain more than 25% of all the angiosperms. Lagerstroemia plants are important ornamental trees which produce big and showy panicles of gorgeous flowers. They commonly bloom from summer till fall and are of significant value in of city landscape and environmental protection. Morphological traits are shared inter-specifically among plants of Lagerstroemia to certain extent and are also influenced by environmental conditions and different developmental stages. Thus, classification of plants in Lagerstroemia at species and cultivar levels is still a challenging task. Chloroplast (cp) genome sequences have been proven to be an informative and valuable source of cp DNA markers for genetic diversity evaluation. In this study, the complete cp genomes of three Lagerstroemia species were newly sequenced, and three other published cp genome sequences of Lagerstroemia were retrieved for comparative analyses in order to obtain an upgraded understanding of the application value of genetic information from the cp genomes. The six cp genomes ranged from 152,049 bp (L. subcostata) to 152,526 bp (L. speciosa) in length. Their organization, gene order and GC content, were similar to the typical cp genome structure of other higher plants. We analyzed nucleotide substitutions, insertions/deletions, and simple sequence repeats in the cp genomes, and discovered 12 relatively highly variable regions that will potentially provide plastid markers for further taxonomic, phylogenetic and population genetics studies in Lagerstroemia. The phylogenetic relationships of the Lagerstroemia taxa inferred from the cp genomes obtained high support, indicating that cp genome data may be useful in resolving relationships in this genus.
Qixiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle
'MDPI AG', 2019Co-Authors: Tangchun Zheng, Ming Cai, Huitang Pan, Tangren Cheng, Jia Wang, Bo Qin, Qixiang ZhangAbstract:Creeping plants have unique ornamental value because they have more branches and flowers and the creeping trait is rare in crape myrtle (Lagerstroemia indica L.). In this study, the first filial generation (F1) population was derived from Lagerstroemia fauriei Koehne (standard) and L. indica “Creole” (creeping) and the backcross1 (BC1) population was derived from the backcross of F1 individual S82 (creeping) and L. fauriei. The segregation of the creeping trait was analyzed for 174 seedlings of the BC1 population to examine the linkage relationship between simple sequence repeat (SSR) molecular markers and the creeping trait. Creeping genes were screened using bulked segregant analysis combined with 322 SSR primers, which were detected with good polymorphism. The results show that two SSR markers (S364 and LYS12) were detected, with genetic distances of 23.49 centimorgan (cM) and 25.86 cM from the loci controlling the plant opening angle trait and the branching angle trait, respectively. The accuracy rate for phenotypic verification using S364 and LYS12 was 76.51% and 74.14%, respectively. Our results provide basic information for the molecular marker-assisted selective breeding and cloning of the creeping gene to improve architecture diversity in the breeding of crape myrtle
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Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa
2018Co-Authors: Tangchun Zheng, Ming Cai, Huitang Pan, Zhilin Chen, Han Zhang, Qixiang ZhangAbstract:Quantitative real-time polymerase chain reaction (qRT-PCR) is a prevalent method for gene expression analysis, depending on the stability of the reference genes for data normalization. Lagerstroemia indica and L. speciosa are popular ornamental plants which are famous for the long flowering period. However, no systematic studies on reference genes in Lagerstroemia have yet been conducted. In the present study, we selected nine candidate reference genes (GAPDH, TUA, TUB, 18S, RPII, EF-1α, ATC, EIF5A and CYP) and evaluated their expression stability in different tissues during floral development of L. indica and L. speciosa using four algorithms (geNorm, NormFinder, BestKeeper and, RefFinder). Results showed that RPII and EF-1α were the most stably expressed and suitable reference genes for both of Lagerstroemia species. Moreover, ACT exhibited high expression stability in L. indica and GAPDH was a suitable reference gene for L. speciosa in different flower development stages. TUB was an unsuitable reference gene for gene expression normalization due to significant variations in expression across all samples. Finally, we verified the reliability of the selected candidate reference genes by amplifying an AGAMOUS homolog (LsAG1) of Arabidopsis thaliana. This study provides a list of suitable reference genes, thereby broadening the genetic basis of the gene expression patterns in Lagerstroemia species.
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Transcriptome analysis of the genes regulating phytohormone and cellular patterning in Lagerstroemia plant architecture
Nature Publishing Group, 2018Co-Authors: Lu Feng, Qixiang Zhang, Ming Cai, Tangchun Zheng, Tangren Cheng, Jia Wang, Huitang PanAbstract:Abstract Plant architecture is a popular research topic because plants with different growth habits that may generate economic or ornamental value are in great demand by orchards and nurseries. However, the molecular basis of the architecture of woody perennial plants is poorly understood due to the complexity of the phenotypic and regulatory relationships. Here, transcriptional profiling of dwarf and non-dwarf crapemyrtles was performed, and potential target genes were identified based on the phenotype, histology and phytohormone metabolite levels. An integrated analysis demonstrated that the internode length was explained mainly by cell number and secondarily by cell length and revealed important hormones in regulatory pathway of Lagerstroemia architecture. Differentially expressed genes (DEGs) involved in phytohormone pathways and cellular patterning regulation were analysed, and the regulatory relationships between these parameters were evaluated at the transcriptional level. Exogenous indole-3-acetic acid (IAA) and gibberellin A4 (GA4) treatments further indicated the pivotal role of auxin in cell division within the shoot apical meristem (SAM) and suggested an interaction between auxin and GA4 in regulating the internode length of Lagerstroemia. These results provide insights for further functional genomic studies on the regulatory mechanisms underlying Lagerstroemia plant architecture and may improve the efficiency of woody plant molecular breeding
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identification and validation of snp markers linked to dwarf traits using slaf seq technology in Lagerstroemia
PLOS ONE, 2016Co-Authors: Ming Cai, Huitang Pan, Lu Feng, Tangren Cheng, Yao Jiao, Qixiang ZhangAbstract:The genetic control of plant architecture is a promising approach to breed desirable cultivars, particularly in ornamental flowers. In this study, the F1 population (142 seedlings) derived from Lagerstroemia fauriei (non-dwarf) × L. indica 'Pocomoke' (dwarf) was phenotyped for six traits (plant height (PH), internode length (IL), internode number, primary lateral branch height (PLBH), secondary lateral branch height and primary branch number), and the IL and PLBH traits were positively correlated with the PH trait and considered representative indexes of PH. Fifty non-dwarf and dwarf seedlings were pooled and subjected to a specific-locus amplified fragment sequencing (SLAF-seq) method, which screened 1221 polymorphic markers. A total of 3 markers segregating between bulks were validated in the F1 population, with the M16337 and M38412 markers highly correlated with the IL trait and the M25207 marker highly correlated with the PLBH trait. These markers provide a predictability of approximately 80% using a single marker (M25207) and a predictability of 90% using marker combinations (M16337 + M25207) in the F1 population, which revealed that the IL and the PLBH traits, especially the PLBH, were the decisive elements for PH in terms of molecular regulation. Further validation was performed in the BC1 population and a set of 28 Lagerstroemia stocks using allele-specific PCR (AS-PCR) technology, and the results showed the stability and reliability of the SNP markers and the co-determination of PH by multiple genes. Our findings provide an important theoretical and practical basis for the early prediction and indirect selection of PH using the IL and the PLBH, and the detected SNPs may be useful for marker-assisted selection (MAS) in crape myrtle.
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screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis
Genetics and Molecular Research, 2015Co-Authors: Yuanjun Ye, J S Shen, Yiqian Ju, X M Bian, Dan He, Qixiang ZhangAbstract:: Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ornamental traits. To understand the inheritance of plant height and screen for genes modulating plant height in Lagerstroemia, segregation of the plant height trait was analyzed using the F1 population of L. fauriei (standard) x L. indica 'Pocomoke' (dwarf) with 96 seedlings, while dwarf genes were screened using the bulked segregant analysis method, combined with 28 amplified fragment length polymorphism primers and 41 simple sequence repeat primers. The results showed that the dwarf trait of crape myrtle was controlled by a major gene and modified by minor genes. An amplified fragment length polymorphism marker, M53E39-92, which was 23.33 cM from the loci controlling the dwarf trait, was screened. These results provide basic information for marker-assisted selection in Lagerstromia and cloning of dwarf genes in future studies.
Luke R Tembrock - One of the best experts on this subject based on the ideXlab platform.
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characterize the complete chloroplast genome of Lagerstroemia floribunda lythraceae a narrow endemic crape myrtle native to southeast asia
Conservation Genetics Resources, 2017Co-Authors: Luke R Tembrock, Dong ZhangAbstract:Lagerstroemia floribunda, commonly known as Thai crape myrtle or kedah bungor, is a species of flowering plant in the Lythraceae family native to tropical regions of Southeast Asia. The complete chloroplast genome of L. floribunda is described in this study. The length of the chloroplast genome was 152,240 bp. The genome consisted of a large single-copy (LSC) region of 83,967 bp, a small single-copy (SSC) region of 33,191 bp and two inverted repeats (IRs) of 17,541 bp, which divide the LSC and SSC regions. This genome contained 112 unique genes, including 78 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Among these genes, 82 were located in the LSC region (22 tRNA genes and 60 protein-coding genes), 13 in the SSC region (one tRNA gene and 12 protein-coding genes), and 17 in both IR regions (7 tRNA genes, six coding genes and four rRNA genes). Sixteen genes had introns, including three protein-coding genes with two introns each (clpP, rps12 and ycf3), eight protein-coding genes with a single intron each (atpF, ndhA, ndhB, petB, petD, rpl16, rpoC1 and rps16), and five tRNA genes with a single intron each (trnA-GUC, trnG-UCC, trnI-GAU, trnK-UUU, trnL-UAA and trnV-UAC). A neighbour-joining tree was reconstructed to indicate relationships among taxa within Myrtales. The newly sequenced complete chloroplast genome of L. floribunda will improve our knowledge of the Lagerstroemia chloroplast and potentially aid conservation efforts for this species.
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the complete chloroplast genome of Lagerstroemia intermedia lythraceae a threatened species endemic to southwestern yunnan province china
Conservation Genetics Resources, 2017Co-Authors: Luke R TembrockAbstract:Lagerstroemia intermedia: is a threatened species endemic to southwestern Yunnan province, China. It has high-value light purple-blue petals. Not much is known about the ecological and genetic characteristics of the species. Chloroplast genome sequencing is an efficient tool for understanding enigmatic and basal phylogenetic relationships at different taxonomic levels. Here, we report the chloroplast genome of L. intermedia. The L. intermedia chloroplast genome is 152,330 bp long, with 129 coding genes, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Among the 129 genes, 6 coding genes (ndhB, rpl2, rpl23, rps7, rps12, and ycf2), 7 tRNA genes (trnA-UGC, trnI-CAU, trnI-GAU, trnL-CAA, trnN-GUU, trnR-ACG, and trnV-GAC) and 4 rRNA genes (rrn4.5, rrn5, rrn16, and rrn23) occur in the two IR regions. A neighbour-joining (NJ) phylogenetic tree was constructed to show the relationships between species in Myrtales. This complete chloroplast genome of L. intermedia will supply more genetic information about the Lagerstroemia chloroplast and will be useful for further research and conservation of this endemic endangered plant.
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the complete chloroplast genome of queen s crape myrtle Lagerstroemia macrocarpa
Mitochondrial DNA Part B, 2016Co-Authors: Luke R TembrockAbstract:The whole complete chloroplast genome of Lagerstroemia macrocarpa was assembled in this study. Total genome is 152,472 bp in length consisting of two inverted repeats of 17,562 bp separated by a large single-copy region and a small single-copy region of 84,050 bp and 33,295 bp, respectively. This genome contains 112 unique genes including 78 protein-coding genes, 4 ribosomal RNA genes and 30 transfer RNA genes. In 78 protein-coding genes, 8 genes (atpF, ndhA, ndhB, petB, petD, rpl16, rpoC1, rps16) contain one intron and three genes with two introns each (clpP, rps12 and ycf3). This newly sequenced chloroplast genome supply highly variable information of polymorphisms within Lagerstroemia species.
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Chloroplast Genome Sequence of Lagerstroemia guilinensis (Lythraceae, Myrtales), a Species Endemic to the Guilin Limestone Area in Guangxi Province, China.
Genome announcements, 2016Co-Authors: Luke R TembrockAbstract:ABSTRACT We announce here the first complete chloroplast genome sequence of Lagerstroemia guilinensis (Lythraceae, Myrtales), a species endemic to the Guilin limestone area, along with its genome structure and functional gene annotations. The plant was collected from Guilin, Guangxi, China, and deposited as a germplasm accession of the Zhejiang Agriculture and Forestry University Collection (ZAFU 1507144). This genome will provide valuable information for future research of the Lagerstroemia genus and its relatives.
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cadmium accumulation and tolerance of Lagerstroemia indica and Lagerstroemia fauriei lythraceae seedlings for phytoremediation applications
International Journal of Phytoremediation, 2016Co-Authors: Yixiang Wang, Shangbin Bai, Zhibin Sun, Tingting Zhu, Xudan Zhu, Dale H Grit, Luke R TembrockAbstract:Contamination by heavy metals is one of the most serious environmental problems generated from human activities. Because phytoremediation utilizes plants to uptake contaminants, it could potentially be used to remediate metal-contaminated areas. A pot culture experiment with four levels of cadmium (Cd) (0, 20, 40, and 80 mg of Cd/kg dry soil) was conducted to investigate Cd accumulation and tolerance of roots, shoots, and leaves of Lagerstroemia indica and Lagerstroemia fauriei as well as their potential for phytoremediation. Experimental results indicated that Cd inhibited seedling growth only at the higher Cd exposure concentration (40 and 80 mg/kg). The tolerance index revealed that on average L. indica is more tolerant of Cd than L. fauriei. Moreover, plants in the experiment accumulated Cd differentially. In comparisons between L. indica and L. fauriei, the leaves of the former had higher concentrations of Cd, while the roots of latter had higher concentrations of Cd. Furthermore, the roots, shoots, and leaves had very high bioaccumulation factors that markedly exceeded 1.0 (exceptional only in shoots of 80 mg/kg for L. fauriei), indicating that the seedlings extracted Cd from the soil. The leaves' translocation factor of L. indica was greater than 1.0, being significantly higher than that of L. fauriei. Chlorophyll a, Chlorophyll b and total declined in both species significantly as Cd concentrations exceeded 40 mg/kg in the soil. In contrast, lipid peroxidation and proline content was found to increase with increasing Cd concentration. From the assessments of biomass production, Cd tolerance and uptake L. indica and L. fauriei could stand as excellent species for remediating Cd-contaminated soils.
Wang Zinan - One of the best experts on this subject based on the ideXlab platform.
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Temperature-Dependent Development and Host Range of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae)
LSU Digital Commons, 2019Co-Authors: Wang Zinan, Chen Yan, Diaz RodrigoAbstract:© 2019 Florida Entomological Society. All rights reserved. The crapemyrtle bark scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is an invasive pest of crapemyrtles, Lagerstroemia spp. L. (Lythraceae) in the southeastern USA. Information about its temperature-dependent development and host range is limited. The objectives of this study were to evaluate the effects of temperature on the immature development of A. Lagerstroemiae, and to determine plant species suitable for immature development and reproduction. Developmental time and survival of eggs were evaluated at 7 constant temperatures from 17.5 to 32 °C, and of nymphs at 20, 25, and 30 °C. Results suggested that 27.5 °C was the optimum temperature for egg hatching with the shortest time (10 d) and the highest hatching rate (95%). The developmental time for A. Lagerstroemiae from nymph to prepupa and gravid female was 56 d and 68 d at 30 °C, respectively. Five plant species besides crapemyrtle (Lagerstroemia indica × fauriei L.) were able to support the immature development and reproduction of A. Lagerstroemiae under no-choice conditions, including Lawsonia inermis L., Heimia salicifolia Link, Punica granatum L., Lythrum alatum Pursh (all Lythraceae), and Callicarpa americana L. (Lamiaceae). At wk 12 from inoculation, the density of gravid females was 482 ± 92 (mean ± standard error) on L. indica × fauriei, 200 ± 70 on C. americana, and \u3c 150 on other species. Using data from developmental time and host range, integrated pest management (IPM) practitioners can implement preventive strategies for A. Lagerstroemiae
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Physiology of crapemyrtle bark scale, Acanthococcus Lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance
LSU Digital Commons, 2019Co-Authors: Wang Zinan, Chen Yan, Diaz Rodrigo, Laine, Roger A.Abstract:© 2018 Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects. Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury. Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea. The crapemyrtle bark scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp. (Myrtales: Lythraceae). Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. Lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points. In addition, time required to achieve the same levels of mortality at lower temperatures also increased. In this study, we used A. Lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month. Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%). The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased. Among all measured polyols and sugars, including glycerol, D-mannitol, myo-inositol, and D-trehalose, the levels of D-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016. Results from this study provide a better understanding on how A. Lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects
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Biology and Ecology of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae)
LSU Digital Commons, 2017Co-Authors: Wang ZinanAbstract:The crapemyrtle bark scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is an exotic pest on crapemyrtles, Lagerstroemia spp. (Myrtales: Lythraceae). Because of its recent arrival in the US, little is known about its biology and ecology. The purpose of my thesis was to improve the knowledge about A. Lagerstroemiae in four aspects involving its thermal tolerance, physiological adaptations to cold temperatures, temperature-dependent development and host range. Thermal tolerance was determined to understand how temperature extremes constrain the distribution of A. Lagerstroemiae in the US. Results suggested that A. Lagerstroemiae can tolerant high heat, but its potential distribution to the northern US may be limited by cold temperatures. Based on laboratory experiments and local temperatures from reported infestations, A. Lagerstroemiae can establish in areas south of 43 °N, which is similar to the northern distribution limit of crapemyrtles. Therefore, the temperature extremes cannot limit its distribution on crapemyrtles in the US. To adapt to winter, cold tolerance of A. Lagerstroemiae nymphs was observed to increase since November. The mechanisms of this increase were investigated by measuring seasonal changes of biochemical variables. From November to February, A. Lagerstroemiae had 20% less water and higher energy reserves, which could have contributed to the increased cold tolerance. A restructuring of fatty acid composition in the body fat of overwintering nymphs was reported indicating accumulation of fatty acids in shorter chains (C6:0, C8:0 and C10:0), resulting in lower melting points that can help maintain lipid fluidity for energy conversion. The development and host range of A. Lagerstroemiae were also studied. Developmental time and survival of A. Lagerstroemiae eggs and nymphs were assessed under different temperatures, and results can help IPM practitioners improve field sampling strategies and timing of control measures. Callicarpa americana L. (Lamiales: Lamiaceae), Heimia salicifolia Link, Lawsonia inermis L., Lythrum alatum Pursh, and Punica granatum L. (Myrtales: Lythraceae) supported life cycle development and reproduction of A. Lagerstroemiae and thus determined as suitable hosts other than Lagerstroemia spp. Scouting is recommended on these host species, following immediate responses to avoid additional spread, economic loss, and ecological disturbance of this pest
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Crapemyrtle bark scale: A new threat for crapemyrtles, a popular landscape plant in the U.S
LSU Digital Commons, 2016Co-Authors: Wang Zinan, Chen Yan, Gu Mengmeng, Vafaie Erfan, Merchant Michael, Diaz RodrigoAbstract:© 2016 by the authors; licensee MDPI, Basel, Switzerland. Crapemyrtle bark scale, Acanthococcus (=Eriococcus) Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced insect pest on crapemyrtles, Lagerstroemia spp. (Myrtales: Lythraceae), one of the most popular flowering shrubs in the U.S. Since first detected in Texas in 2004, this pest has spread to twelve states causing losses to stakeholders. To develop a management plan, we reviewed current knowledge about the pest’s biology and ecology, and suggested research approaches including studying its thermal tolerance, host range, plant resistance and biological control. Parasitoids and predators have been reared from A. Lagerstroemiae in the U.S. and China. However, new surveys of natural enemies should be conducted in China, and studies on the host range and impacts of natural enemies on A. Lagerstroemiae may help determine the potential for classical biological control. The life history, preying efficiency and rearing methods are important for coccinellid predators found in the U.S. including Chilocorus cacti L. and Hyperaspis spp. To enhance natural enemy performance, it is important to evaluate a sustainable insecticide program that considers efficacy, timing, rate and impact on pollinator health. Finally, an integrated management program of A. Lagerstroemiae is discussed including planting resistant cultivars, using host specific natural enemies, and prudent use of insecticides
Wenpan Dong - One of the best experts on this subject based on the ideXlab platform.
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chloroplast phylogenomics and divergence times of Lagerstroemia lythraceae
BMC Genomics, 2021Co-Authors: Wenpan Dong, Yanlei Liu, Jipu Shi, Zhili SuoAbstract:Crape myrtles, belonging to the genus Lagerstroemia L., have beautiful paniculate inflorescences and are cultivated as important ornamental tree species for landscaping and gardening. However, the phylogenetic relationships within Lagerstroemia have remained unresolved likely caused by limited sampling and the insufficient number of informative sites used in previous studies. In this study, we sequenced 20 Lagerstroemia chloroplast genomes and combined with 15 existing chloroplast genomes from the genus to investigate the phylogenetic relationships and divergence times within Lagerstroemia. The phylogenetic results indicated that this genus is a monophyletic group containing four clades. Our dating analysis suggested that Lagerstroemia originated in the late Paleocene (~ 60 Ma) and started to diversify in the middle Miocene. The diversification of most species occurred during the Pleistocene. Four variable loci, trnD-trnY-trnE, rrn16-trnI, ndhF-rpl32-trnL and ycf1, were discovered in the Lagerstroemia chloroplast genomes. The chloroplast genome information was successfully utilized for molecular characterization of diverse crape myrtle samples. Our results are valuable for the global genetic diversity assessment, conservation and utilization of Lagerstroemia.
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Chloroplast phylogenomics and divergence times of Lagerstroemia (Lythraceae)
'Springer Science and Business Media LLC', 2021Co-Authors: Wenpan Dong, Yanlei Liu, Jipu Shi, Zhili SuoAbstract:Abstract Background Crape myrtles, belonging to the genus Lagerstroemia L., have beautiful paniculate inflorescences and are cultivated as important ornamental tree species for landscaping and gardening. However, the phylogenetic relationships within Lagerstroemia have remained unresolved likely caused by limited sampling and the insufficient number of informative sites used in previous studies. Results In this study, we sequenced 20 Lagerstroemia chloroplast genomes and combined with 15 existing chloroplast genomes from the genus to investigate the phylogenetic relationships and divergence times within Lagerstroemia. The phylogenetic results indicated that this genus is a monophyletic group containing four clades. Our dating analysis suggested that Lagerstroemia originated in the late Paleocene (~ 60 Ma) and started to diversify in the middle Miocene. The diversification of most species occurred during the Pleistocene. Four variable loci, trnD-trnY-trnE, rrn16-trnI, ndhF-rpl32-trnL and ycf1, were discovered in the Lagerstroemia chloroplast genomes. Conclusions The chloroplast genome information was successfully utilized for molecular characterization of diverse crape myrtle samples. Our results are valuable for the global genetic diversity assessment, conservation and utilization of Lagerstroemia
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comparative analysis of six Lagerstroemia complete chloroplast genomes
Frontiers in Plant Science, 2017Co-Authors: Wenpan Dong, Jipu Shi, Xiaoman Xie, Xiaobai Jin, Zhili SuoAbstract:Crape myrtles are economically important tree species of the genus Lagerstroemia L. (Lythraceae), with a distribution from tropical to northern temperate zones. They are positioned phylogenetically to a large subclade of rosids (in the eudicots) which contain more than 25% of all the angiosperms. Lagerstroemia plants are important ornamental trees which produce big and showy panicles of gorgeous flowers. They commonly bloom from summer till fall and are of significant value in of city landscape and environmental protection. Morphological traits are shared inter-specifically among plants of Lagerstroemia to certain extent and are also influenced by environmental conditions and different developmental stages. Thus, classification of plants in Lagerstroemia at species and cultivar levels is still a challenging task. Chloroplast (cp) genome sequences have been proven to be an informative and valuable source of cp DNA markers for genetic diversity evaluation. In this study, the complete cp genomes of three Lagerstroemia species were newly sequenced, and three other published cp genome sequences of Lagerstroemia were retrieved for comparative analyses in order to obtain an upgraded understanding of the application value of genetic information from the cp genomes. The six cp genomes ranged from 152,049 bp (L. subcostata) to 152,526 bp (L. speciosa) in length. Their organization, gene order and GC content, were similar to the typical cp genome structure of other higher plants. We analyzed nucleotide substitutions, insertions/deletions, and simple sequence repeats in the cp genomes, and discovered 12 relatively highly variable regions that will potentially provide plastid markers for further taxonomic, phylogenetic and population genetics studies in Lagerstroemia. The phylogenetic relationships of the Lagerstroemia taxa inferred from the cp genomes obtained high support, indicating that cp genome data may be useful in resolving relationships in this genus.
