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J V Odoherty - One of the best experts on this subject based on the ideXlab platform.
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effects of dietary supplementation with a Laminarin rich extract on the growth performance and gastrointestinal health in broilers
Poultry Science, 2021Co-Authors: B Venardou, J V Odoherty, M T Ryan, Stafford Vigors, C J Oshea, E J Burton, T SweeneyAbstract:Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development. The objective of this study was to determine the effects of dietary inclusion of Laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens. Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm Laminarin; (T3) basal diet + 300 ppm Laminarin (5 bird/pen; 16 pens/treatment). The basal diet was supplemented with a Laminarin-rich Laminaria spp. extract (65% Laminarin) to achieve the two Laminarin inclusion levels (150 and 300 ppm). Chick weights and feed intake was recorded weekly. After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) Laminarin groups were euthanized. Duodenal, jejunal and ileal tissues were collected for gene expression analysis. Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR). Dietary supplementation with 300 ppm Laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups. Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum. Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05). In conclusion, supplementation with Laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.
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molecular characteristics and antioxidant activity of Laminarin extracted from the seaweed species laminaria hyperborea using hydrothermal assisted extraction and a multi step purification procedure
Food Hydrocolloids, 2021Co-Authors: Gaurav Rajauria, Dilip K. Rai, T Sweeney, Rajeev Ravindran, Marco Garciavaquero, J V OdohertyAbstract:Abstract The objective of this study was to determine the effect of hydrothermal-assisted extraction (HAE) and a multi-step purification process on the molecular characteristics and antioxidant activity of extracts of Laminarin from the brown seaweed Laminaria hyperborea. The HAE with optimised extraction conditions (time - 30 min; temperature - 99.3 °C; sample to solvent ratio - 1:21.3 (w/v)) resulted in 368 mg/g Laminarin in the Crude Extract. Purification using solvents, molecular weight cut-off filter (MWCO; 10 kDa) and solid-phase extraction (SPE) improved the concentration to 542.6 mg/g, 906.6 mg/g and 862.5 mg/g on dry weight extract basis, respectively (p 0.05). The identification of a chromatographic peak at 17.66 min (retention time) and FT-IR (Fourier transform infrared) absorption bands at 1420 cm−1 (carboxyl groups), 2950 cm−1 (C-H stretch) and at 2410 cm−1 (transmitting angle peak) was similar between the purified samples and a Laminarin standard that was used as a control, confirming the presence of Laminarin. The quadruple time of flight mass spectrometry (Q-ToF-MS) confirmed the molecular weight of purified Laminarin was in the range of 5.7 kDa–6.2 kDa. HAE under optimised conditions was an effective method to recover biologically active Laminarin from L. hyperborea. A multi-step purification, involving solvents and MWCO filters in a sequence could improve the purity of Laminarin, however, purification reduced the antioxidant activity compared to the crude HAE extract.
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the effects of the marine derived polysaccharides Laminarin and chitosan on aspects of colonic health in pigs challenged with dextran sodium sulphate
Marine Drugs, 2020Co-Authors: Ruth Rattigan, J V Odoherty, M T Ryan, Gaurav Rajauria, Stafford Vigors, Kevin Thornton, Rocco S Sebastiano, John J Callanan, Lekha Menon Margassery, Alan D W DobsonAbstract:This study examined the effects of dietary supplementation with Laminarin or chitosan on colonic health in pigs challenged with dextran sodium sulphate (DSS). Weaned pigs were assigned to: (1) a basal diet (n = 22); (2) a basal diet + Laminarin (n = 10); and (3) a basal diet + chitosan (n = 10). On d35, the basal group was split, creating four groups: (1) the basal diet (control); (2) the basal diet + DSS; (3) the basal diet + Laminarin + DSS; and (4) the basal diet + chitosan + DSS. From d39–42, the pigs were orally challenged with DSS. On d44, colonic tissue/digesta samples were collected. The basal DSS group had reduced growth, higher pathology score and an increased expression of MMP1, IL13 and IL23 compared with the controls (p 0.05). In the basal DSS group, the relative abundance of beneficial taxa including Prevotella and Roseburia were reduced while Escherichia/Shigella were increased, compared with the controls (p < 0.05). The relative abundance of Escherichia/Shigella was reduced and the molar proportions of acetate were increased in the Laminarin DSS group compared with the basal DSS group (p < 0.01), suggesting that Laminarin has potential to prevent pathogen proliferation and enhance the volatile fatty acid profile in the colon in a porcine model of colitis.
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effect of a Laminarin rich macroalgal extract on the caecal and colonic microbiota in the post weaned pig
Marine Drugs, 2020Co-Authors: J V Odoherty, Gaurav Rajauria, Stafford Vigors, Ruth Rattigan, Mary J Mcdonnell, T SweeneyAbstract:Dietary supplementation with 300 ppm of a Laminarin rich macroalgal extract reduces post-weaning intestinal dysfunction in pigs. A comprehensive analysis of the impact of Laminarin on the intestinal microbiome during this period is essential to inform on the mode of action of this bioactivity. The objective of this study was to evaluate the effects of supplementing the diet of newly weaned pigs with 300 ppm of a Laminarin rich extract, on animal performance, volatile fatty acids, and the intestinal microbiota using 16S rRNA gene sequencing. Pigs fed the Laminarin-supplemented diet had higher average daily feed intake, growth rate, and body weight compared to pigs fed the control diet (p < 0.05). Pigs fed the Laminarin-supplemented diet had reduced abundance of OTUs assigned to Enterobacteriaceae and increased abundance of OTUs assigned to the genus Prevotella (p < 0.05) compared to pigs fed the control diet. Enterobacteriaceae had negative relationships (p < 0.05) with average daily feed intake (ADFI), average daily gain (ADG), and butyric acid concentrations. In contrast, Prevotellaceae were positively correlated (p < 0.05) with ADFI, ADG, total VFA, acetic, propionic, butyric acids, and negatively correlated with isovaleric acid. Hence supplementation with a Laminarin enriched extract potentially improves performance during the post-weaning period by promoting the proliferation of bacterial taxa such as Prevotella that favourably enhance nutrient digestion while reducing the load of potentially pathogenic bacterial taxa including Enterobacteriaceae.
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Laminarin rich extract improves growth performance small intestinal morphology gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post weaning period
British Journal of Nutrition, 2020Co-Authors: Ruth Rattigan, T Sweeney, Gaurav Rajauria, Siofra E Maher, Kevin Thornton, J V OdohertyAbstract:The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry. The objective of this experiment was to determine the effects of increasing dietary inclusion levels of Laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction. At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) Laminarin; (3) basal + 200 ppm Laminarin and (4) basal + 300 ppm Laminarin (three pigs/pen). The appropriate quantity of a Laminarin-rich extract (65 % Laminarin) was added to the basal diet to achieve the above dietary inclusion levels of Laminarin. After 14 d of supplementation, eight pigs from the basal group and the best-performing Laminarin group were euthanised for sample collection. The 300 ppm Laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05). Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05). Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon. Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon. In conclusion, 300 ppm Laminarin from a Laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
T Sweeney - One of the best experts on this subject based on the ideXlab platform.
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effects of dietary supplementation with a Laminarin rich extract on the growth performance and gastrointestinal health in broilers
Poultry Science, 2021Co-Authors: B Venardou, J V Odoherty, M T Ryan, Stafford Vigors, C J Oshea, E J Burton, T SweeneyAbstract:Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development. The objective of this study was to determine the effects of dietary inclusion of Laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens. Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm Laminarin; (T3) basal diet + 300 ppm Laminarin (5 bird/pen; 16 pens/treatment). The basal diet was supplemented with a Laminarin-rich Laminaria spp. extract (65% Laminarin) to achieve the two Laminarin inclusion levels (150 and 300 ppm). Chick weights and feed intake was recorded weekly. After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) Laminarin groups were euthanized. Duodenal, jejunal and ileal tissues were collected for gene expression analysis. Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR). Dietary supplementation with 300 ppm Laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups. Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum. Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05). In conclusion, supplementation with Laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.
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molecular characteristics and antioxidant activity of Laminarin extracted from the seaweed species laminaria hyperborea using hydrothermal assisted extraction and a multi step purification procedure
Food Hydrocolloids, 2021Co-Authors: Gaurav Rajauria, Dilip K. Rai, T Sweeney, Rajeev Ravindran, Marco Garciavaquero, J V OdohertyAbstract:Abstract The objective of this study was to determine the effect of hydrothermal-assisted extraction (HAE) and a multi-step purification process on the molecular characteristics and antioxidant activity of extracts of Laminarin from the brown seaweed Laminaria hyperborea. The HAE with optimised extraction conditions (time - 30 min; temperature - 99.3 °C; sample to solvent ratio - 1:21.3 (w/v)) resulted in 368 mg/g Laminarin in the Crude Extract. Purification using solvents, molecular weight cut-off filter (MWCO; 10 kDa) and solid-phase extraction (SPE) improved the concentration to 542.6 mg/g, 906.6 mg/g and 862.5 mg/g on dry weight extract basis, respectively (p 0.05). The identification of a chromatographic peak at 17.66 min (retention time) and FT-IR (Fourier transform infrared) absorption bands at 1420 cm−1 (carboxyl groups), 2950 cm−1 (C-H stretch) and at 2410 cm−1 (transmitting angle peak) was similar between the purified samples and a Laminarin standard that was used as a control, confirming the presence of Laminarin. The quadruple time of flight mass spectrometry (Q-ToF-MS) confirmed the molecular weight of purified Laminarin was in the range of 5.7 kDa–6.2 kDa. HAE under optimised conditions was an effective method to recover biologically active Laminarin from L. hyperborea. A multi-step purification, involving solvents and MWCO filters in a sequence could improve the purity of Laminarin, however, purification reduced the antioxidant activity compared to the crude HAE extract.
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effect of a Laminarin rich macroalgal extract on the caecal and colonic microbiota in the post weaned pig
Marine Drugs, 2020Co-Authors: J V Odoherty, Gaurav Rajauria, Stafford Vigors, Ruth Rattigan, Mary J Mcdonnell, T SweeneyAbstract:Dietary supplementation with 300 ppm of a Laminarin rich macroalgal extract reduces post-weaning intestinal dysfunction in pigs. A comprehensive analysis of the impact of Laminarin on the intestinal microbiome during this period is essential to inform on the mode of action of this bioactivity. The objective of this study was to evaluate the effects of supplementing the diet of newly weaned pigs with 300 ppm of a Laminarin rich extract, on animal performance, volatile fatty acids, and the intestinal microbiota using 16S rRNA gene sequencing. Pigs fed the Laminarin-supplemented diet had higher average daily feed intake, growth rate, and body weight compared to pigs fed the control diet (p < 0.05). Pigs fed the Laminarin-supplemented diet had reduced abundance of OTUs assigned to Enterobacteriaceae and increased abundance of OTUs assigned to the genus Prevotella (p < 0.05) compared to pigs fed the control diet. Enterobacteriaceae had negative relationships (p < 0.05) with average daily feed intake (ADFI), average daily gain (ADG), and butyric acid concentrations. In contrast, Prevotellaceae were positively correlated (p < 0.05) with ADFI, ADG, total VFA, acetic, propionic, butyric acids, and negatively correlated with isovaleric acid. Hence supplementation with a Laminarin enriched extract potentially improves performance during the post-weaning period by promoting the proliferation of bacterial taxa such as Prevotella that favourably enhance nutrient digestion while reducing the load of potentially pathogenic bacterial taxa including Enterobacteriaceae.
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Laminarin rich extract improves growth performance small intestinal morphology gene expression of nutrient transporters and the large intestinal microbial composition of piglets during the critical post weaning period
British Journal of Nutrition, 2020Co-Authors: Ruth Rattigan, T Sweeney, Gaurav Rajauria, Siofra E Maher, Kevin Thornton, J V OdohertyAbstract:The identification of natural bioactive compounds which can prevent the post-weaning growth check and enhance gastrointestinal health in the absence of in-feed medications is an urgent priority for the swine industry. The objective of this experiment was to determine the effects of increasing dietary inclusion levels of Laminarin in the first 14 d post-weaning on pig growth performance and weaning associated intestinal dysfunction. At weaning, ninety-six pigs (8·4 (sd 1·09) kg) (meatline boars × (large white × landrace sows)) were blocked by live weight, litter and sex and randomly assigned to: (1) basal diet; (2) basal + 100 parts per million (ppm) Laminarin; (3) basal + 200 ppm Laminarin and (4) basal + 300 ppm Laminarin (three pigs/pen). The appropriate quantity of a Laminarin-rich extract (65 % Laminarin) was added to the basal diet to achieve the above dietary inclusion levels of Laminarin. After 14 d of supplementation, eight pigs from the basal group and the best-performing Laminarin group were euthanised for sample collection. The 300 ppm Laminarin group was selected as this group had higher ADFI compared with all other groups and higher ADG than the basal group (P < 0·05). Laminarin supplementation increased villus height in the duodenum and jejunum (P < 0·05). Laminarin supplementation increased the expression of SLC2A8/GLUT8 in the duodenum, SLC2A2/GLUT2, SLC2A7/GLUT7, SLC15A1/PEPT1 and FABP2 in the jejunum and SLC16A1/MCT1 in the colon. Laminarin supplementation reduced Enterobacteriaceae numbers in the caecum (P < 0·05) and increased lactobacilli numbers (P < 0·05), total volatile fatty acid concentrations and the molar proportions of butyrate (P < 0·01) in the colon. In conclusion, 300 ppm Laminarin from a Laminarin-rich extract has potential, as a dietary supplement, to improve performance and prevent post-weaning intestinal dysfunction.
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effect of seaweed derived Laminarin and fucoidan and zinc oxide on gut morphology nutrient transporters nutrient digestibility growth performance and selected microbial populations in weaned pigs
British Journal of Nutrition, 2014Co-Authors: G Heim, M T Ryan, T Sweeney, C J Oshea, A M Walsh, D N Doyle, J V OdohertyAbstract:In the present study, two experiments were conducted to (1) evaluate the effect of Laminarin and/or fucoidan on ileal morphology, nutrient transporter gene expression and coefficient of total tract apparent digestibility (CTTAD) of nutrients and (2) determine whether Laminarin inclusion could be used as an alternative to ZnO supplementation in weaned pig diets. Expt 1 was designed as a 2 × 2 factorial arrangement, comprising four dietary treatments (n 7 replicates, weaning age 24 d, live weight 6·9 kg). The dietary treatments were as follows: (1) basal diet; (2) basal diet+300 ppm Laminarin; (3) basal diet+240 ppm fucoidan; (4) basal diet+300 ppm Laminarin and 240 ppm fucoidan. There was an interaction between Laminarin and fucoidan on the CTTAD of gross energy (GE) (P< 0·05) and the expression of sodium–glucose-linked transporter 1 (SGLT1/SLC5A1) and GLUT1/SLC2A1 and GLUT2/SLC2A2 (P< 0·05) in the ileum. The Laminarin diet increased the CTTAD of GE and increased the expression of SGLT1, GLUT1 and GLUT2 compared with the basal diet. However, there was no effect of Laminarin supplementation on these variables when combined with fucoidan. Expt 2 was designed as a complete randomised design (n 8 replicates/treatment, weaning age 24 d, live weight 7·0 kg), and the treatments were (1) basal diet, (2) basal diet and Laminarin (300 ppm), and (3) basal diet and ZnO (3100 ppm, 0–14 d, and 2600 ppm, 15–32 d post-weaning). The Laminarin diet increased average daily gain and gain:feed ratio compared with the basal diet during days 0–32 post-weaning (P< 0·01) and had an effect similar to the ZnO diet. These results demonstrate that Laminarin provides a dietary means to improve gut health and growth performance post-weaning.
Joseph P Kerry - One of the best experts on this subject based on the ideXlab platform.
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influence of level and duration of feeding polysaccharide Laminarin and fucoidan extracts from brown seaweed laminaria digitata on quality indices of fresh pork
Meat Science, 2015Co-Authors: Natasha C Moroney, J V Odoherty, M N Ogrady, Ruairi C Robertson, Catherine Stanton, Joseph P KerryAbstract:Abstract The effect of level (450 or 900 mg Laminarin (L) and fucoidan (F) /kg feed) and duration (3 or 6 wks) of feeding a seaweed (Laminaria digitata) extract containing L/F on the quality of pork (longissimus thoracis et lumborum (LTL)) stored in modified atmosphere packs and on organ lipid stability was examined. Mechanisms of L/F antioxidant activity in LTL were evaluated. Plasma total antioxidant status, LTL pH, colour, microbiology and ‘eating quality’ sensory analysis were unaffected by dietary L/F. ‘Visual’ sensory descriptors (purchasing appeal and overall visual acceptability) were enhanced (p
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effect of a brown seaweed laminaria digitata extract containing Laminarin and fucoidan on the quality and shelf life of fresh and cooked minced pork patties
Meat Science, 2013Co-Authors: J V Odoherty, Natasha C Moroney, M N Ogrady, Joseph P KerryAbstract:Abstract A spray-dried seaweed extract containing Laminarin (L, 9.3%) and fucoidan (F, 7.8%) (L/F extract) from brown seaweed (Laminaria digitata) was added directly to minced pork (M. longissimus dorsi) (LD) at levels of 0.01%, 0.1% and 0.5% (w/w). Fresh and cooked minced pork patties were stored in modified atmosphere packs containing 80% O2:20% CO2 and 70% N2:30% CO2, respectively, for up to 14 days at 4 °C. The L/F extract reduced the surface redness (‘a*’ values) of fresh patties as a function of concentration. The L/F extract (0.5%) exerted the greatest lipid pro-oxidant activity in fresh patties. The L/F extract (0.5%) significantly decreased (P
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addition of seaweed laminaria digitata extracts containing Laminarin and fucoidan to porcine diets influence on the quality and shelf life of fresh pork
Meat Science, 2012Co-Authors: J V Odoherty, Natasha C Moroney, M N Ogrady, Joseph P KerryAbstract:Abstract A seaweed extract containing Laminarin (L) and fucoidan (F) (L/F) was manufactured from brown seaweed ( Laminaria digitata ) in spray-dried (L/F-SD) and wet (L/F-WS) forms. The effect of supplementation of pig diets with L/F-SD and L/F-WS (L, 500 mg/kg feed; F, 420 mg/kg feed) for 21 days pre-slaughter, on quality indices of fresh M. longissimus dorsi (LD) steaks was examined. Susceptibility of porcine liver, heart, kidney and lung tissue homogenates to iron-induced (1 mM FeSO 4 ) lipid oxidation was also investigated. Dietary supplementation with L/F did not increase plasma total antioxidant status (TAS). In LD steaks stored in modified atmosphere packs (80% O 2 :20% CO 2 ) (MAP) for up to 15 days at 4 °C, muscle pH, surface colour (CIE ‘ L *’ lightness, ‘ a *’ redness and ‘ b *’ yellowness values) and microbiology (psychrotrophic and mesophilic counts, log CFU/g pork) were unaffected by dietary L/F. In general, levels of lipid oxidation (TBARS, mg MDA (malondialdehyde)/kg pork) followed the order: C > LF-SD > L/F-WS. A statistically significant reduction in lipid oxidation (P via the animal's diet.
Natasha C Moroney - One of the best experts on this subject based on the ideXlab platform.
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influence of level and duration of feeding polysaccharide Laminarin and fucoidan extracts from brown seaweed laminaria digitata on quality indices of fresh pork
Meat Science, 2015Co-Authors: Natasha C Moroney, J V Odoherty, M N Ogrady, Ruairi C Robertson, Catherine Stanton, Joseph P KerryAbstract:Abstract The effect of level (450 or 900 mg Laminarin (L) and fucoidan (F) /kg feed) and duration (3 or 6 wks) of feeding a seaweed (Laminaria digitata) extract containing L/F on the quality of pork (longissimus thoracis et lumborum (LTL)) stored in modified atmosphere packs and on organ lipid stability was examined. Mechanisms of L/F antioxidant activity in LTL were evaluated. Plasma total antioxidant status, LTL pH, colour, microbiology and ‘eating quality’ sensory analysis were unaffected by dietary L/F. ‘Visual’ sensory descriptors (purchasing appeal and overall visual acceptability) were enhanced (p
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effect of a brown seaweed laminaria digitata extract containing Laminarin and fucoidan on the quality and shelf life of fresh and cooked minced pork patties
Meat Science, 2013Co-Authors: J V Odoherty, Natasha C Moroney, M N Ogrady, Joseph P KerryAbstract:Abstract A spray-dried seaweed extract containing Laminarin (L, 9.3%) and fucoidan (F, 7.8%) (L/F extract) from brown seaweed (Laminaria digitata) was added directly to minced pork (M. longissimus dorsi) (LD) at levels of 0.01%, 0.1% and 0.5% (w/w). Fresh and cooked minced pork patties were stored in modified atmosphere packs containing 80% O2:20% CO2 and 70% N2:30% CO2, respectively, for up to 14 days at 4 °C. The L/F extract reduced the surface redness (‘a*’ values) of fresh patties as a function of concentration. The L/F extract (0.5%) exerted the greatest lipid pro-oxidant activity in fresh patties. The L/F extract (0.5%) significantly decreased (P
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addition of seaweed laminaria digitata extracts containing Laminarin and fucoidan to porcine diets influence on the quality and shelf life of fresh pork
Meat Science, 2012Co-Authors: J V Odoherty, Natasha C Moroney, M N Ogrady, Joseph P KerryAbstract:Abstract A seaweed extract containing Laminarin (L) and fucoidan (F) (L/F) was manufactured from brown seaweed ( Laminaria digitata ) in spray-dried (L/F-SD) and wet (L/F-WS) forms. The effect of supplementation of pig diets with L/F-SD and L/F-WS (L, 500 mg/kg feed; F, 420 mg/kg feed) for 21 days pre-slaughter, on quality indices of fresh M. longissimus dorsi (LD) steaks was examined. Susceptibility of porcine liver, heart, kidney and lung tissue homogenates to iron-induced (1 mM FeSO 4 ) lipid oxidation was also investigated. Dietary supplementation with L/F did not increase plasma total antioxidant status (TAS). In LD steaks stored in modified atmosphere packs (80% O 2 :20% CO 2 ) (MAP) for up to 15 days at 4 °C, muscle pH, surface colour (CIE ‘ L *’ lightness, ‘ a *’ redness and ‘ b *’ yellowness values) and microbiology (psychrotrophic and mesophilic counts, log CFU/g pork) were unaffected by dietary L/F. In general, levels of lipid oxidation (TBARS, mg MDA (malondialdehyde)/kg pork) followed the order: C > LF-SD > L/F-WS. A statistically significant reduction in lipid oxidation (P via the animal's diet.
Taekjeong Nam - One of the best experts on this subject based on the ideXlab platform.
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induction of apoptosis and the regulation of erbb signaling by Laminarin in ht 29 human colon cancer cells
International Journal of Molecular Medicine, 2013Co-Authors: Heekyoung Park, Inhye Kim, Joong Kyun Kim, Taekjeong NamAbstract:Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which Laminarin from Laminaria digitata induces apoptosis in HT-29 colon cancer cells, as well as the involvement of the ErbB signaling pathway. Cell viability assay revealed that Laminarin induced cell death in a dose-dependent manner. Cell cycle analysis revealed that Laminarin increased the percentage of cells in the sub-G1 and G2-M phase. Western blot analysis demonstrated that Laminarin inhibited the heregulin-stimulated phosphorylation of ErbB2. A decrease in cellular proliferation was also observed; this was found to be dependent on ErbB, which activates c-Jun N-terminal kinase. These findings demonstrate the important role of the epidermal growth factor receptor in colon cancer tumorigenesis, and suggest the potential of Laminarin as a bio-functional food with anticancer effects on human colon cancer.
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induction of apoptosis by Laminarin regulating the insulin like growth factor ir signaling pathways in ht 29 human colon cells
International Journal of Molecular Medicine, 2012Co-Authors: Heekyoung Park, Inhye Kim, Joong Kyun Kim, Taekjeong NamAbstract:In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of Laminarin on HT-29 cells and analyzed its effect on the insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed that Laminarin induced cell death in a dose-dependent manner. Western blotting showed that Laminarin decreased mitogen-activated protein kinases (MAPK) and ERK phosphorylation. Decreased proliferation depended on IGF-IR, which was associated with the downregulation of MAPK/ERK. These results are important for understanding the roles of IGF-IR in colon cancer cell tumorigenesis, and suggest that Laminarin shows activity against human colon cancer.
