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Sudarshan Ojha - One of the best experts on this subject based on the ideXlab platform.
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evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves
BMC Research Notes, 2014Co-Authors: Sanjiv Kumar, Rajat Sandhir, Sudarshan OjhaAbstract:Phytochemicals like carotenoids, tocopherols, ascorbates and phenols present in the plants are strong antioxidants and have an important role in the health care system. There is growing interest in correlating the phytochemical constituents of a plant with its pharmacological activity. Therefore, the present study investigates the content of total phenolics, flavonoids and the antioxidant activity of four different varieties of Lantana camara L. (Verbenaceae) leaves by using in vitro antioxidant models. The leaves of Chandigarh purple variety (CPV), Palampur red variety (PRV), Chandigarh yellow turning pink variety (YTPV) and Chandigarh yellow variety (CYV) Lantana camara were collected and the total phenolic, flavonoid content, antioxidant and free radical scavenging activities were determined in their methanolic extracts. The phenolic content was found to be highest in the CYV extract (232.99 ± 15.97 mg GAE/ g extract). The content of the flavonoids are in the order of YTPV, PRV, CPV and CYV. The IC50 values for the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test were in the order of CYV (33.30 ± 2.39) PRV > YTPV > CPV. The IC50 values of 2, 2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) scavenging assay for CYV, PRV, YTPV, CPV were 18.25 ± 0.19, 18.24 ± 1.82, 50.43 ± 9.49, 52.84 ± 1.82 μg/mL respectively. PRV extract showed the maximum in vitro lipid peroxidation inhibition effect with an IC50 value of 68.50 μg/mL which is even stronger as compared to the standard Rutin (79.69 μg/mL). The extracts showed a strong correlation between the phenolic content and their antioxidant activities. The highest correlation (r = 0.998, R2 = 0.997) was found between total phenolic content and ABTS scavenging assay. Among the four varieties investigated, CYV and PRV extracts showed strong antioxidant activities and may be used as a potential source of natural antioxidant against free radical associated diseases.
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evaluation of antimotility effect of Lantana camara l var acuelata constituents on neostigmine induced gastrointestinal transit in mice
BMC Complementary and Alternative Medicine, 2005Co-Authors: Lenika Sagar, Rajesh Sehgal, Sudarshan OjhaAbstract:Lantana camara L. (Verbenaceae), a widely growing shrub which is toxic to some animal species, has been used in the traditional medicine for treating many ailments. The purpose of the present study was to evaluate the antimotility effects of Lantana camara leaf constituents in mice intestine. Evaluation of antimotility activity was done in intestine of mice treated with Lantana camara leaf powder, Lantana camara methanolic extract (LCME), lantadene A, neostigmine and neostigmine + LCME. Neostigmine was used as a promotility agent. Intestinal motility was assessed by charcoal meal test and gastrointestinal transit rate was expressed as the percentage of the distance traversed by the charcoal divided by the total length of the small intestine. The antidiarrheal effect of LCME was studied against castor oil induced diarrhea model in mice. The intestinal transit with LCME at a dose of 500 mg/kg was 26.46% whereas the higher dose (1 g/kg) completely inhibited the transit of charcoal in normal mice. The % intestinal transit in the neostigmine pretreated groups was 24 and 11 at the same doses respectively. When the plant extracts at 125 and 250 mg/kg doses were administered intraperitonealy, there was significant reduction in fecal output compared with castor oil treated mice. At higher doses (500 and 1000 mg/kg), the fecal output was almost completely stopped. The remarkable antimotility effect of Lantana camara methanolic extract against neostigmine as promotility agent points towards an anticholinergic effect due to Lantana camara constituents and attests to its possible utility in secretory and functional diarrheas and other gastrointestinal disorders. This effect was further confirmed by significant inhibition of castor oil induced diarrhea in mice by various doses of LCME.
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Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice
BMC Complementary and Alternative Medicine, 2005Co-Authors: Lenika Sagar, Rajesh Sehgal, Sudarshan OjhaAbstract:Background Lantana camara L. (Verbenaceae), a widely growing shrub which is toxic to some animal species, has been used in the traditional medicine for treating many ailments. The purpose of the present study was to evaluate the antimotility effects of Lantana camara leaf constituents in mice intestine. Methods Evaluation of antimotility activity was done in intestine of mice treated with Lantana camara leaf powder, Lantana camara methanolic extract (LCME), lantadene A, neostigmine and neostigmine + LCME. Neostigmine was used as a promotility agent. Intestinal motility was assessed by charcoal meal test and gastrointestinal transit rate was expressed as the percentage of the distance traversed by the charcoal divided by the total length of the small intestine. The antidiarrheal effect of LCME was studied against castor oil induced diarrhea model in mice. Results The intestinal transit with LCME at a dose of 500 mg/kg was 26.46% whereas the higher dose (1 g/kg) completely inhibited the transit of charcoal in normal mice. The % intestinal transit in the neostigmine pretreated groups was 24 and 11 at the same doses respectively. When the plant extracts at 125 and 250 mg/kg doses were administered intraperitonealy, there was significant reduction in fecal output compared with castor oil treated mice. At higher doses (500 and 1000 mg/kg), the fecal output was almost completely stopped. Conclusion The remarkable antimotility effect of Lantana camara methanolic extract against neostigmine as promotility agent points towards an anticholinergic effect due to Lantana camara constituents and attests to its possible utility in secretory and functional diarrheas and other gastrointestinal disorders. This effect was further confirmed by significant inhibition of castor oil induced diarrhea in mice by various doses of LCME.
Sreedhara P Reddy - One of the best experts on this subject based on the ideXlab platform.
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Lantana camara leaf extract mediated silver nanoparticles antibacterial green catalyst
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: B Ajitha, Ashok Kumar Y Reddy, Syed Shameer, K M Rajesh, Y Suneetha, Sreedhara P ReddyAbstract:Abstract Silver nanoparticles (AgNPs) have been synthesized by Lantana camara leaf extract through simple green route and evaluated their antibacterial and catalytic activities. The leaf extract (LE) itself acts as both reducing and stabilizing agent at once for desired nanoparticle synthesis. The colorless reaction mixture turns to yellowish brown attesting the AgNPs formation and displayed UV–Vis absorption spectra. Structural analysis confirms the crystalline nature and formation of fcc structured metallic silver with majority (1 1 1) facets. Morphological studies elicit the formation of almost spherical shaped nanoparticles and as AgNO 3 concentration is increased, there is an increment in the particle size. The FTIR analysis evidences the presence of various functional groups of biomolecules of LE is responsible for stabilization of AgNPs. Zeta potential measurement attests the higher stability of synthesized AgNPs. The synthesized AgNPs exhibited good antibacterial activity when tested against Escherichia coli , Pseudomonas spp., Bacillus spp. and Staphylococcus spp. using standard Kirby–Bauer disc diffusion assay. Furthermore, they showed good catalytic activity on the reduction of methylene blue by L. camara extract which is monitored and confirmed by the UV–Vis spectrophotometer.
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green synthesis and characterization of silver nanoparticles using Lantana camara leaf extract
Materials Science and Engineering: C, 2015Co-Authors: B Ajitha, Ashok Kumar Y Reddy, Sreedhara P ReddyAbstract:In this work, we have investigated on Lantana camara mediated silver nanoparticles (AgNPs) with different leaf extract (LE) quantity for the evaluation of efficient bactericidal activity. The AgNPs were prepared by simple, capable, eco-friendly and biosynthesis method using L. camara LE. This method allowed the synthesis of crystalline nanoparticles, which was confirmed by X-ray diffraction (XRD) and selected area electron diffraction (SAED) patterns. The X-ray photoelectron spectroscopy (XPS) analysis confirmed the formation of metallic silver and elucidates the surface state composition of AgNPs. UV-vis spectra of AgNPs and visual perception of brownish yellow color from colorless reaction mixture confirmed the AgNP formation. Involvement of functional groups of L. camara leaf extract in the reduction and capping process of nanoparticles was well displayed in Fourier transform infrared spectroscopy (FTIR). Decrement of particle size with an increment of leaf extract volume was evident in AFM, TEM images and also through a blue shift in the UV-vis spectra. The rate of formation and size of AgNPs were dependent on LE quantity. Meanwhile, these AgNPs exhibited effective antibacterial activity with the decrement of particle size against all tested bacterial cultures.
Lenika Sagar - One of the best experts on this subject based on the ideXlab platform.
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evaluation of antimotility effect of Lantana camara l var acuelata constituents on neostigmine induced gastrointestinal transit in mice
BMC Complementary and Alternative Medicine, 2005Co-Authors: Lenika Sagar, Rajesh Sehgal, Sudarshan OjhaAbstract:Lantana camara L. (Verbenaceae), a widely growing shrub which is toxic to some animal species, has been used in the traditional medicine for treating many ailments. The purpose of the present study was to evaluate the antimotility effects of Lantana camara leaf constituents in mice intestine. Evaluation of antimotility activity was done in intestine of mice treated with Lantana camara leaf powder, Lantana camara methanolic extract (LCME), lantadene A, neostigmine and neostigmine + LCME. Neostigmine was used as a promotility agent. Intestinal motility was assessed by charcoal meal test and gastrointestinal transit rate was expressed as the percentage of the distance traversed by the charcoal divided by the total length of the small intestine. The antidiarrheal effect of LCME was studied against castor oil induced diarrhea model in mice. The intestinal transit with LCME at a dose of 500 mg/kg was 26.46% whereas the higher dose (1 g/kg) completely inhibited the transit of charcoal in normal mice. The % intestinal transit in the neostigmine pretreated groups was 24 and 11 at the same doses respectively. When the plant extracts at 125 and 250 mg/kg doses were administered intraperitonealy, there was significant reduction in fecal output compared with castor oil treated mice. At higher doses (500 and 1000 mg/kg), the fecal output was almost completely stopped. The remarkable antimotility effect of Lantana camara methanolic extract against neostigmine as promotility agent points towards an anticholinergic effect due to Lantana camara constituents and attests to its possible utility in secretory and functional diarrheas and other gastrointestinal disorders. This effect was further confirmed by significant inhibition of castor oil induced diarrhea in mice by various doses of LCME.
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Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice
BMC Complementary and Alternative Medicine, 2005Co-Authors: Lenika Sagar, Rajesh Sehgal, Sudarshan OjhaAbstract:Background Lantana camara L. (Verbenaceae), a widely growing shrub which is toxic to some animal species, has been used in the traditional medicine for treating many ailments. The purpose of the present study was to evaluate the antimotility effects of Lantana camara leaf constituents in mice intestine. Methods Evaluation of antimotility activity was done in intestine of mice treated with Lantana camara leaf powder, Lantana camara methanolic extract (LCME), lantadene A, neostigmine and neostigmine + LCME. Neostigmine was used as a promotility agent. Intestinal motility was assessed by charcoal meal test and gastrointestinal transit rate was expressed as the percentage of the distance traversed by the charcoal divided by the total length of the small intestine. The antidiarrheal effect of LCME was studied against castor oil induced diarrhea model in mice. Results The intestinal transit with LCME at a dose of 500 mg/kg was 26.46% whereas the higher dose (1 g/kg) completely inhibited the transit of charcoal in normal mice. The % intestinal transit in the neostigmine pretreated groups was 24 and 11 at the same doses respectively. When the plant extracts at 125 and 250 mg/kg doses were administered intraperitonealy, there was significant reduction in fecal output compared with castor oil treated mice. At higher doses (500 and 1000 mg/kg), the fecal output was almost completely stopped. Conclusion The remarkable antimotility effect of Lantana camara methanolic extract against neostigmine as promotility agent points towards an anticholinergic effect due to Lantana camara constituents and attests to its possible utility in secretory and functional diarrheas and other gastrointestinal disorders. This effect was further confirmed by significant inhibition of castor oil induced diarrhea in mice by various doses of LCME.
B Ajitha - One of the best experts on this subject based on the ideXlab platform.
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Lantana camara leaf extract mediated silver nanoparticles antibacterial green catalyst
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: B Ajitha, Ashok Kumar Y Reddy, Syed Shameer, K M Rajesh, Y Suneetha, Sreedhara P ReddyAbstract:Abstract Silver nanoparticles (AgNPs) have been synthesized by Lantana camara leaf extract through simple green route and evaluated their antibacterial and catalytic activities. The leaf extract (LE) itself acts as both reducing and stabilizing agent at once for desired nanoparticle synthesis. The colorless reaction mixture turns to yellowish brown attesting the AgNPs formation and displayed UV–Vis absorption spectra. Structural analysis confirms the crystalline nature and formation of fcc structured metallic silver with majority (1 1 1) facets. Morphological studies elicit the formation of almost spherical shaped nanoparticles and as AgNO 3 concentration is increased, there is an increment in the particle size. The FTIR analysis evidences the presence of various functional groups of biomolecules of LE is responsible for stabilization of AgNPs. Zeta potential measurement attests the higher stability of synthesized AgNPs. The synthesized AgNPs exhibited good antibacterial activity when tested against Escherichia coli , Pseudomonas spp., Bacillus spp. and Staphylococcus spp. using standard Kirby–Bauer disc diffusion assay. Furthermore, they showed good catalytic activity on the reduction of methylene blue by L. camara extract which is monitored and confirmed by the UV–Vis spectrophotometer.
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green synthesis and characterization of silver nanoparticles using Lantana camara leaf extract
Materials Science and Engineering: C, 2015Co-Authors: B Ajitha, Ashok Kumar Y Reddy, Sreedhara P ReddyAbstract:In this work, we have investigated on Lantana camara mediated silver nanoparticles (AgNPs) with different leaf extract (LE) quantity for the evaluation of efficient bactericidal activity. The AgNPs were prepared by simple, capable, eco-friendly and biosynthesis method using L. camara LE. This method allowed the synthesis of crystalline nanoparticles, which was confirmed by X-ray diffraction (XRD) and selected area electron diffraction (SAED) patterns. The X-ray photoelectron spectroscopy (XPS) analysis confirmed the formation of metallic silver and elucidates the surface state composition of AgNPs. UV-vis spectra of AgNPs and visual perception of brownish yellow color from colorless reaction mixture confirmed the AgNP formation. Involvement of functional groups of L. camara leaf extract in the reduction and capping process of nanoparticles was well displayed in Fourier transform infrared spectroscopy (FTIR). Decrement of particle size with an increment of leaf extract volume was evident in AFM, TEM images and also through a blue shift in the UV-vis spectra. The rate of formation and size of AgNPs were dependent on LE quantity. Meanwhile, these AgNPs exhibited effective antibacterial activity with the decrement of particle size against all tested bacterial cultures.
O P Sharma - One of the best experts on this subject based on the ideXlab platform.
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a review of the hepatotoxic plant Lantana camara
Critical Reviews in Toxicology, 2007Co-Authors: O P Sharma, Vasantha Pattabhi, Sarita Sharma, Shashi B Mahato, Pritam Dev SharmaAbstract:Lantana (Lantana camara Linn) is a noxious weed that grows in many tropical and subtropical parts of the world. Ingestion of Lantana foliage by grazing animals causes cholestasis and hepatotoxicity...
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molecular structure polymorphism and toxicity of lantadene a the pentacyclic triterpenoid from the hepatotoxic plant Lantana camara
Journal of Biochemical Toxicology, 1991Co-Authors: O P Sharma, Rajinder K Dawra, Vasantha PattabhiAbstract:: Lantadene A (22 beta-angeloyloxy-3-oxo-olean-12-en-28-oic acid), a pentacyclic triterpenoid compound from Lantana (Lantana camara) leaves has been obtained in two polymorphic forms I and II. Form I had white, fluffy, and rod-shaped uniform crystals. Form II particles were irregular, shining, and polyhedral. The two forms differed in melting behavior. The powder x-ray diffraction of form I showed sharp peaks whereas from II did not contain distinct peaks. From single-crystal three-dimensional x-ray structure determination, the molecular structure of form I has been established. A/B and B/C rings of the molecule are trans fused while D/E rings are cis fused. The packing of the molecule is stabilized by hydrogen bonding. Form I of lantadene A was non-toxic to guinea pigs on oral administration. Form II induced ictericity and toxicity associated with decrease in feed intake and fecal output, hepatomegaly, increase in plasma bilirubin, and acid phosphatase activity.
