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Sengottayan Senthil Nathan - One of the best experts on this subject based on the ideXlab platform.
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the use of eucalyptus tereticornis sm myrtaceae oil leaf extract as a natural Larvicidal Agent against the malaria vector anopheles stephensi liston diptera culicidae
Bioresource Technology, 2007Co-Authors: Sengottayan Senthil NathanAbstract:Secondary metabolites obtained from the indigenous plants with proven mosquito control potential can be used as an alternative to synthetic insecticides under the integrated vector control. The essential oil extract from the forest redgum, Eucalyptus tereticornis Sm. (Myrtaceae) was tested against mature and immature mosquito vector Anopheles stephensi Liston (Diptera) under laboratory condition. The extract showed strong Larvicidal, pupicidal and adulticidal activity. The leaf oil extracts showed high bioactivity at high doses. Results obtained from the laboratory experiment showed that the leaf extracts suppressed the pupal and adult activity of Anopheles stephensi at higher doses. In general, first and second instar larvae were more susceptible to all treatments. Clear dose -response relationships were established with the highest dose of 160ppm plant extract evoking almost 100% mortality. The results obtained suggest that, in addition to their medicinal activities, E. tereticornis can also serve as a natural mosquitocide.
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short communication the use of eucalyptus tereticornis sm myrtaceae oil leaf extract as a natural Larvicidal Agent against the malaria vector anopheles stephensi liston diptera culicidae
2007Co-Authors: Sengottayan Senthil NathanAbstract:Secondary metabolites obtained from the indigenous plants with proven mosquito control potential can be used as an alternative to synthetic insecticides under the integrated vector control. The essential oil extract from the forest redgum, Eucalyptus tereticornis Sm. (Myrtaceae) was tested against mature and immature mosquito vector Anopheles stephensi Liston (Diptera) under laboratory condition. The extract showed strong Larvicidal, pupicidal and adulticidal activity. The leaf oil extracts showed high bioactivity at high doses. Results obtained from the laboratory experiment showed that the leaf extracts suppressed the pupal and adult activity of Anopheles stephensi at higher doses. In general, first and second instar larvae were more susceptible to all treatments. Clear dose –response relationships were established with the highest dose of 160 ppm plant extract evoking almost 100% mortality. The results obtained suggest that, in addition to their medicinal activities, E. tereticornis can also serve as a natural mosquitocide. � 2006 Elsevier Ltd. All rights reserved.
Patrícia Maria Guedes Paiva - One of the best experts on this subject based on the ideXlab platform.
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schinus terebinthifolius leaf extract causes midgut damage interfering with survival and development of aedes aegypti larvae
PLOS ONE, 2015Co-Authors: Thamara Figueiredo Procopio, Daniela Maria Do Amaral Ferraz Navarro, Patrícia Maria Guedes Paiva, Emmanuel Viana Pontual, Kenner Morais Fernandes, Rafael Matos Ximenes, Aline Rafaella Cardoso De Oliveira, Carolina De Santana Souza, Ana Maria Mendonca De Albuquerque Melo, Gustavo Ferreira MartinsAbstract:In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3–1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in Larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The Larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution should be used when employing this extract as a Larvicidal Agent.
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trypsin inhibitor from moringa oleifera flowers interferes with survival and development of aedes aegypti larvae and kills bacteria inhabitant of larvae midgut
Parasitology Research, 2014Co-Authors: Emmanuel Viana Pontual, Daniela Maria Do Amaral Ferraz Navarro, Nataly Diniz De Lima Santos, Thiago Henrique Napoleão, Luana C B B Coelho, Maiara Celine De Moura, Patrícia Maria Guedes PaivaAbstract:Moringa oleifera flower extract, with trypsin in- hibitor activity, is a Larvicidal Agent on Aedes aegypti .T his work reports the isolation of trypsin inhibitor (M. oleifera flower trypsin inhibitor (MoFTI)) and its effect on A. aegypti egg hatching, viability of newly hatched larvae, survival of pupae, and growth of inhabitant bacteria from midgut of fourth-instar larvae (L4). MoFTI (Ki ,2 .4μM), isolated by affinity chromatography on trypsin-agarose column, was an 18.2 kDa polypeptide on sodium dodecyl sulphate- polyacrylamidegelelectrophoresis.Flowerextract(at concen- trations of 8.5-17.0 mg/mL) reduced egg hatchability while MoFTI (0.05-0.5 mg/mL) did not affect the hatching rate. Mortality of newly hatched larvae ranged from 3.5 to 19.1 % in the presence of the extract (4.0-17.0 mg/mL) and was also promoted by MoFTI (LC50, 0.3 mg/mL). After 72 h, larvae incubated with extract at 13.0 and 17.0 mg/mL were at stages L2 and L1, respectively, while in control they reached L3 instar. In the presence of MoFTI, at all concentrations tested, the larvae did not pass the first instar. Flower extract and MoFTI did not interfere on pupae survival. The extract and MoFTI inhibited the growth of L4 gut bacteria (minimum inhibitory concentrations of 3.47 and 0.031 mg/mL, respec- tively) but only the inhibitor showed bactericide effect (min- imum bactericidal concentration of 1.0 mg/mL). The findings reported herein indicate that MoFTI constitutes a Larvicidal principle from M. oleifera flowers against A. aegypti newly hatched larvae and is an antibacterial Agent active against the microbiota from L4 gut.
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oviposition stimulant and ovicidal activities of moringa oleifera lectin on aedes aegypti
PLOS ONE, 2012Co-Authors: Nataly Diniz De Lima Santos, Geanne K.n. Santos, Daniela Maria Do Amaral Ferraz Navarro, Kézia Santana De Moura, Thiago Henrique Napoleão, Luana C B B Coelho, Patrícia Maria Guedes PaivaAbstract:Background Natural insecticides against the vector mosquito Aedes aegypti have been the object of research due to their high level of eco-safety. The water-soluble Moringa oleifera lectin (WSMoL) is a Larvicidal Agent against A. aegypti. This work reports the effects of WSMoL on oviposition and egg hatching of A. aegypti. Methodology/Principal Findings WSMoL crude preparations (seed extract and 0–60 protein fraction), at 0.1 mg/mL protein concentration, did not affect oviposition, while A. aegypti gravid females laid their eggs preferentially (73%) in vessels containing isolated WSMoL (0.1 mg/mL), compared with vessels containing only distilled water (control). Volatile compounds were not detected in WSMoL preparation. The hatchability of fresh eggs deposited in the solutions in the oviposition assay was evaluated. The numbers of hatched larvae in seed extract, 0–60 protein fraction and WSMoL were 45±8.7 %, 20±11 % and 55±7.5 %, respectively, significantly (p<0.05) lower than in controls containing only distilled water (75–95%). Embryos were visualized inside fresh control eggs, but not within eggs that were laid and maintained in WSMoL solution. Ovicidal activity was also assessed using stored A. aegypti eggs. The protein concentrations able to reduce the hatching rate by 50% (EC50) were 0.32, 0.16 and 0.1 mg/mL for seed extract, 0–60 protein fraction and WSMoL, respectively. The absence of hatching of stored eggs treated with WSMoL at 0.3 mg/mL (EC99) after transfer to medium without lectin indicates that embryos within the eggs were killed by WSMoL. The reduction in hatching rate of A. aegypti was not linked to decrease in bacteria population. Conclusions/Significance WSMoL acted both as a chemical stimulant cue for ovipositing females and ovicidal Agent at a given concentration. The oviposition-stimulant and ovicidal activities, combined with the previously reported Larvicidal activity, make WSMoL a very interesting candidate in integrated A. aegypti control.
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effect of myracrodruon urundeuva leaf lectin on survival and digestive enzymes of aedes aegypti larvae
Parasitology Research, 2012Co-Authors: Thiago Henrique Napoleão, Daniela Maria Do Amaral Ferraz Navarro, Nataly Diniz De Lima Santos, Emmanuel Viana Pontual, Thâmarah De Albuquerque Lima, Luana C B B Coelho, Patrícia Maria Guedes PaivaAbstract:Aedes aegypti transmits the viruses that cause yellow and dengue fevers. Vector control is essential, since a vaccine for dengue has not as yet been made available. This work reports on the Larvicidal activity of Myracrodruon urundeuva leaf lectin (MuLL) against A. aegypti fourth-stage larvae (L4). Also, the resistance of MuLL to digestion by L4 gut proteases and the effects of MuLL on protease, trypsin-like and α-amylase activities from L4 gut were evaluated to determine if lectin remains active in A. aegypti gut and if insect enzyme activities can be modulated by MuLL. MuLL promoted mortality of L4 with LC50 of 0.202 mg/ml. Haemagglutinating activity of MuLL was detected even after incubation for 96 h with L4 gut preparation containing protease activity. MuLL affected the activity of gut enzymes, inhibiting protease and trypsin activities and stimulating α-amylase activity. The results suggest that MuLL may become a new biodegradable Larvicidal Agent for dengue control. Larvicidal activity of MuLL may be linked to its resistance to proteolysis by larval enzymes and interference in the activity of digestive larval enzymes.
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Oviposition-stimulant and ovicidal activities of Moringa oleifera lectin on Aedes aegypti.
Public Library of Science (PLoS), 2024Co-Authors: Nataly Diniz De Lima Santos, Daniela Maria Do Amaral Ferraz Navarro, Kézia Santana De Moura, Thiago Henrique Napoleão, Geanne Karla Novais Santos, Luana Cassandra Breitenbach Barroso Coelho, Patrícia Maria Guedes PaivaAbstract:Natural insecticides against the vector mosquito Aedes aegypti have been the object of research due to their high level of eco-safety. The water-soluble Moringa oleifera lectin (WSMoL) is a Larvicidal Agent against A. aegypti. This work reports the effects of WSMoL on oviposition and egg hatching of A. aegypti.WSMoL crude preparations (seed extract and 0-60 protein fraction), at 0.1 mg/mL protein concentration, did not affect oviposition, while A. aegypti gravid females laid their eggs preferentially (73%) in vessels containing isolated WSMoL (0.1 mg/mL), compared with vessels containing only distilled water (control). Volatile compounds were not detected in WSMoL preparation. The hatchability of fresh eggs deposited in the solutions in the oviposition assay was evaluated. The numbers of hatched larvae in seed extract, 0-60 protein fraction and WSMoL were 45 ± 8.7 %, 20 ± 11 % and 55 ± 7.5 %, respectively, significantly (p
V Venkatesalu - One of the best experts on this subject based on the ideXlab platform.
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Larvicidal potential of acorus calamus l essential oil against filarial vector mosquito culex quinquefasciatus diptera culicidae
Asian Pacific Journal of Tropical Disease, 2012Co-Authors: A Senthilkumar, V VenkatesaluAbstract:Abstract Objective To identify the chemical composition and Larvicidal activity of Acorus calamus ( A. calamus ) rhizome essential oil against the filarial vector mosquito, Culex quinquefasciatus ( Cx. quinquefasciatus ). Methods Essential oil was isolated by hydro-distillation and the chemical composition of the oil was analysed by gas chromatography and gas chromatography mass spectroscopy analysis. The Larvicidal activity of the essential oil was analysed at different concentrations, viz ., 12.5, 25.0, 50.0, 100.0 and 200.0 mL/L. Early 4th instar larvae were used for the Larvicidal assay. The larval mortality was calculated after 24 h of the exposure. Results The gas chromatography and gas chromatography mass spectroscopy analysis showed that the essential oil extracted from the rhizome of A. calamus contained 20 chemical compounds representing about 99.99% of the total oil. Beta-asarone (33.36%), cis -beta-terpineol (23.44%), limonene (13.08%), carvone (5.64%) and amyl isovalerate (4.92%) were identified as the major chemical compounds. The essential oil had promising Larvicidal effect against the early 4th instar larvae of Cx. quinquefasciatus with LC 50 value of 63.43 mL/L and LC 90 value of 145.95 mL/L. Conclusion The essential oil of A. calamus rhizome can be used as a natural Larvicidal Agent against the larvae of filarial vector mosquito, Cx. quinquefasciatus .
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chemical composition and Larvicidal activity of the essential oil of plectranthus amboinicus lour spreng against anopheles stephensi a malarial vector mosquito
Parasitology Research, 2010Co-Authors: A Senthilkumar, V VenkatesaluAbstract:Essential oil of Plectranthus amboinicus was studied for its chemical composition and Larvicidal potential against the malarial vector mosquito Anopheles stephensi. Totally 26 compounds were identified by GC and GC-MS. The major chemical compounds were carvacrol (28.65%) followed by thymol (21.66%), α-humulene (9.67%), undecanal (8.29%), γ-terpinene (7.76%), ρ-cymene (6.46%), caryophyllene oxide (5.85%), α-terpineol (3.28%) and β-selinene (2.01%). The Larvicidal assay was conducted to record the LC50 and LC90 values and the larval mortality was observed after 12 and 24 h of exposure period. The LC50 values of the oil were 33.54 (after 12 h) and 28.37 ppm (after 24 h). The LC90 values of the oil were 70.27 (after 12 h) and 59.38 ppm (after 24 h). The results of the present study showed that the essential oil of P. amboinicus is one of the inexpensive and eco-friendly sources of natural mosquito Larvicidal Agent to control/reduce the population of malarial vector mosquito.
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chemical constituents and Larvicidal property of the essential oil of blumea mollis d don merr against culex quinquefasciatus
Parasitology Research, 2008Co-Authors: A Senthilkumar, Krishnan Kannathasan, V VenkatesaluAbstract:The essential oil from the leaves of Blumea mollis was extracted and the chemical constituents and the Larvicidal against Culex quinquefasciatus effects studied. The analyses of gas chromatography (GC) and GC–mass spectroscopy (GC–MS) revealed that the leaf essential oil of B. mollis contained 39 compounds, and the major chemical compounds identified were linalool (19.43%), γ-elemene (12.19%), copaene (10.93%), estragole (10.81%), Allo-ocimene (10.03%), γ-terpinene (8.28%) and Allo-aromadendrene (7.44%). The essential oil had significant toxic effect against early fourth-instar larvae of C. quinquefasciatus with LC50 = 71.71 and LC90 = 143.41 ppm. The results could be useful in search of newer, safer and more effective natural Larvicidal Agent against C. quinquefasciatus.
Daniela Maria Do Amaral Ferraz Navarro - One of the best experts on this subject based on the ideXlab platform.
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schinus terebinthifolius leaf extract causes midgut damage interfering with survival and development of aedes aegypti larvae
PLOS ONE, 2015Co-Authors: Thamara Figueiredo Procopio, Daniela Maria Do Amaral Ferraz Navarro, Patrícia Maria Guedes Paiva, Emmanuel Viana Pontual, Kenner Morais Fernandes, Rafael Matos Ximenes, Aline Rafaella Cardoso De Oliveira, Carolina De Santana Souza, Ana Maria Mendonca De Albuquerque Melo, Gustavo Ferreira MartinsAbstract:In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3–1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in Larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The Larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution should be used when employing this extract as a Larvicidal Agent.
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Extract of Bowdichia virgilioides and maackiain as Larvicidal Agent against Aedes aegypti mosquito
Experimental Parasitology, 2015Co-Authors: Patrícia Cristina Bezerra-silva, Jefferson C. Santos, Geanne K.n. Santos, Kamilla A. Dutra, Andréa Lopes Bandeira Delmiro Santana, Claudia A. Maranhão, Márcia Silva Do Nascimento, Daniela Maria Do Amaral Ferraz Navarro, Lothar W. BieberAbstract:The Larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest Larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/mL. Fraction toluene/EtOAc (8:2) from this extract showed Larvicidal activity (LC50 = 34.90 ± 1.27 µg/mL). A mixture of two compounds identified as medicarpin and maackiain exhibited a very good Larvicidal activity (sub-fraction 2, LC50 = 17.5 ± 1.87 µg/mL) and maackiain showed to be a strong Larvicidal compound (LC50 = 21.95 ± 1.34 µg/mL). This result can be of value in the search for new natural Larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.
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trypsin inhibitor from moringa oleifera flowers interferes with survival and development of aedes aegypti larvae and kills bacteria inhabitant of larvae midgut
Parasitology Research, 2014Co-Authors: Emmanuel Viana Pontual, Daniela Maria Do Amaral Ferraz Navarro, Nataly Diniz De Lima Santos, Thiago Henrique Napoleão, Luana C B B Coelho, Maiara Celine De Moura, Patrícia Maria Guedes PaivaAbstract:Moringa oleifera flower extract, with trypsin in- hibitor activity, is a Larvicidal Agent on Aedes aegypti .T his work reports the isolation of trypsin inhibitor (M. oleifera flower trypsin inhibitor (MoFTI)) and its effect on A. aegypti egg hatching, viability of newly hatched larvae, survival of pupae, and growth of inhabitant bacteria from midgut of fourth-instar larvae (L4). MoFTI (Ki ,2 .4μM), isolated by affinity chromatography on trypsin-agarose column, was an 18.2 kDa polypeptide on sodium dodecyl sulphate- polyacrylamidegelelectrophoresis.Flowerextract(at concen- trations of 8.5-17.0 mg/mL) reduced egg hatchability while MoFTI (0.05-0.5 mg/mL) did not affect the hatching rate. Mortality of newly hatched larvae ranged from 3.5 to 19.1 % in the presence of the extract (4.0-17.0 mg/mL) and was also promoted by MoFTI (LC50, 0.3 mg/mL). After 72 h, larvae incubated with extract at 13.0 and 17.0 mg/mL were at stages L2 and L1, respectively, while in control they reached L3 instar. In the presence of MoFTI, at all concentrations tested, the larvae did not pass the first instar. Flower extract and MoFTI did not interfere on pupae survival. The extract and MoFTI inhibited the growth of L4 gut bacteria (minimum inhibitory concentrations of 3.47 and 0.031 mg/mL, respec- tively) but only the inhibitor showed bactericide effect (min- imum bactericidal concentration of 1.0 mg/mL). The findings reported herein indicate that MoFTI constitutes a Larvicidal principle from M. oleifera flowers against A. aegypti newly hatched larvae and is an antibacterial Agent active against the microbiota from L4 gut.
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oviposition stimulant and ovicidal activities of moringa oleifera lectin on aedes aegypti
PLOS ONE, 2012Co-Authors: Nataly Diniz De Lima Santos, Geanne K.n. Santos, Daniela Maria Do Amaral Ferraz Navarro, Kézia Santana De Moura, Thiago Henrique Napoleão, Luana C B B Coelho, Patrícia Maria Guedes PaivaAbstract:Background Natural insecticides against the vector mosquito Aedes aegypti have been the object of research due to their high level of eco-safety. The water-soluble Moringa oleifera lectin (WSMoL) is a Larvicidal Agent against A. aegypti. This work reports the effects of WSMoL on oviposition and egg hatching of A. aegypti. Methodology/Principal Findings WSMoL crude preparations (seed extract and 0–60 protein fraction), at 0.1 mg/mL protein concentration, did not affect oviposition, while A. aegypti gravid females laid their eggs preferentially (73%) in vessels containing isolated WSMoL (0.1 mg/mL), compared with vessels containing only distilled water (control). Volatile compounds were not detected in WSMoL preparation. The hatchability of fresh eggs deposited in the solutions in the oviposition assay was evaluated. The numbers of hatched larvae in seed extract, 0–60 protein fraction and WSMoL were 45±8.7 %, 20±11 % and 55±7.5 %, respectively, significantly (p<0.05) lower than in controls containing only distilled water (75–95%). Embryos were visualized inside fresh control eggs, but not within eggs that were laid and maintained in WSMoL solution. Ovicidal activity was also assessed using stored A. aegypti eggs. The protein concentrations able to reduce the hatching rate by 50% (EC50) were 0.32, 0.16 and 0.1 mg/mL for seed extract, 0–60 protein fraction and WSMoL, respectively. The absence of hatching of stored eggs treated with WSMoL at 0.3 mg/mL (EC99) after transfer to medium without lectin indicates that embryos within the eggs were killed by WSMoL. The reduction in hatching rate of A. aegypti was not linked to decrease in bacteria population. Conclusions/Significance WSMoL acted both as a chemical stimulant cue for ovipositing females and ovicidal Agent at a given concentration. The oviposition-stimulant and ovicidal activities, combined with the previously reported Larvicidal activity, make WSMoL a very interesting candidate in integrated A. aegypti control.
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effect of myracrodruon urundeuva leaf lectin on survival and digestive enzymes of aedes aegypti larvae
Parasitology Research, 2012Co-Authors: Thiago Henrique Napoleão, Daniela Maria Do Amaral Ferraz Navarro, Nataly Diniz De Lima Santos, Emmanuel Viana Pontual, Thâmarah De Albuquerque Lima, Luana C B B Coelho, Patrícia Maria Guedes PaivaAbstract:Aedes aegypti transmits the viruses that cause yellow and dengue fevers. Vector control is essential, since a vaccine for dengue has not as yet been made available. This work reports on the Larvicidal activity of Myracrodruon urundeuva leaf lectin (MuLL) against A. aegypti fourth-stage larvae (L4). Also, the resistance of MuLL to digestion by L4 gut proteases and the effects of MuLL on protease, trypsin-like and α-amylase activities from L4 gut were evaluated to determine if lectin remains active in A. aegypti gut and if insect enzyme activities can be modulated by MuLL. MuLL promoted mortality of L4 with LC50 of 0.202 mg/ml. Haemagglutinating activity of MuLL was detected even after incubation for 96 h with L4 gut preparation containing protease activity. MuLL affected the activity of gut enzymes, inhibiting protease and trypsin activities and stimulating α-amylase activity. The results suggest that MuLL may become a new biodegradable Larvicidal Agent for dengue control. Larvicidal activity of MuLL may be linked to its resistance to proteolysis by larval enzymes and interference in the activity of digestive larval enzymes.
A Senthilkumar - One of the best experts on this subject based on the ideXlab platform.
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Larvicidal potential of acorus calamus l essential oil against filarial vector mosquito culex quinquefasciatus diptera culicidae
Asian Pacific Journal of Tropical Disease, 2012Co-Authors: A Senthilkumar, V VenkatesaluAbstract:Abstract Objective To identify the chemical composition and Larvicidal activity of Acorus calamus ( A. calamus ) rhizome essential oil against the filarial vector mosquito, Culex quinquefasciatus ( Cx. quinquefasciatus ). Methods Essential oil was isolated by hydro-distillation and the chemical composition of the oil was analysed by gas chromatography and gas chromatography mass spectroscopy analysis. The Larvicidal activity of the essential oil was analysed at different concentrations, viz ., 12.5, 25.0, 50.0, 100.0 and 200.0 mL/L. Early 4th instar larvae were used for the Larvicidal assay. The larval mortality was calculated after 24 h of the exposure. Results The gas chromatography and gas chromatography mass spectroscopy analysis showed that the essential oil extracted from the rhizome of A. calamus contained 20 chemical compounds representing about 99.99% of the total oil. Beta-asarone (33.36%), cis -beta-terpineol (23.44%), limonene (13.08%), carvone (5.64%) and amyl isovalerate (4.92%) were identified as the major chemical compounds. The essential oil had promising Larvicidal effect against the early 4th instar larvae of Cx. quinquefasciatus with LC 50 value of 63.43 mL/L and LC 90 value of 145.95 mL/L. Conclusion The essential oil of A. calamus rhizome can be used as a natural Larvicidal Agent against the larvae of filarial vector mosquito, Cx. quinquefasciatus .
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chemical composition and Larvicidal activity of the essential oil of plectranthus amboinicus lour spreng against anopheles stephensi a malarial vector mosquito
Parasitology Research, 2010Co-Authors: A Senthilkumar, V VenkatesaluAbstract:Essential oil of Plectranthus amboinicus was studied for its chemical composition and Larvicidal potential against the malarial vector mosquito Anopheles stephensi. Totally 26 compounds were identified by GC and GC-MS. The major chemical compounds were carvacrol (28.65%) followed by thymol (21.66%), α-humulene (9.67%), undecanal (8.29%), γ-terpinene (7.76%), ρ-cymene (6.46%), caryophyllene oxide (5.85%), α-terpineol (3.28%) and β-selinene (2.01%). The Larvicidal assay was conducted to record the LC50 and LC90 values and the larval mortality was observed after 12 and 24 h of exposure period. The LC50 values of the oil were 33.54 (after 12 h) and 28.37 ppm (after 24 h). The LC90 values of the oil were 70.27 (after 12 h) and 59.38 ppm (after 24 h). The results of the present study showed that the essential oil of P. amboinicus is one of the inexpensive and eco-friendly sources of natural mosquito Larvicidal Agent to control/reduce the population of malarial vector mosquito.
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chemical constituents and Larvicidal property of the essential oil of blumea mollis d don merr against culex quinquefasciatus
Parasitology Research, 2008Co-Authors: A Senthilkumar, Krishnan Kannathasan, V VenkatesaluAbstract:The essential oil from the leaves of Blumea mollis was extracted and the chemical constituents and the Larvicidal against Culex quinquefasciatus effects studied. The analyses of gas chromatography (GC) and GC–mass spectroscopy (GC–MS) revealed that the leaf essential oil of B. mollis contained 39 compounds, and the major chemical compounds identified were linalool (19.43%), γ-elemene (12.19%), copaene (10.93%), estragole (10.81%), Allo-ocimene (10.03%), γ-terpinene (8.28%) and Allo-aromadendrene (7.44%). The essential oil had significant toxic effect against early fourth-instar larvae of C. quinquefasciatus with LC50 = 71.71 and LC90 = 143.41 ppm. The results could be useful in search of newer, safer and more effective natural Larvicidal Agent against C. quinquefasciatus.
