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Bernard La Scola - One of the best experts on this subject based on the ideXlab platform.
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Legionella saoudiensis sp nov., isolated from a sewage water sample
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1(T), was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1(T) was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1(T) belongs to the genus Legionella in the family Legionellaceae. Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6(T) (98.6 %) and Legionella lytica L2(T) (98.5 %). The mip gene sequence of strain LS-1(T) showed 94% sequence similarity with that of L. lytica L2(T) and 93% similarity with that of L. rowbothamii LLAP-6(T). Strain LS-1(T) grew optimally at a temperature of 32 degrees C on a buffered charcoal yeast extract (BCYE) agar plate in a 5% CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1(T) represents a novel species of the genus Legionella, for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1(T) (= DSM 101682(T) = CSUR P2101(T)).
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Legionella saoudiensis sp. nov., isolated from a sewage water sample.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1T, was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1T was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1T belongs to the genus Legionella in the family Legionellaceae . Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6T (98.6 %) and Legionella lytica L2T (98.5 %). The mip gene sequence of strain LS-1T showed 94 % sequence similarity with that of L. lytica L2T and 93 % similarity with that of L. rowbothamii LLAP-6T. Strain LS-1T grew optimally at a temperature of 32 °C on a buffered charcoal yeast extract (BCYE) agar plate in a 5 % CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1T represents a novel species of the genus Legionella , for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1T (=DSM 101682T=CSUR P2101T).
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Genome Sequence of Legionella anisa, Isolated from a Respiratory Sample, Using an Amoebal Coculture Procedure.
Genome announcements, 2014Co-Authors: Isabelle Pagnier, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella anisa is a gammaproteobacterium from the class Legionellaceae, which is responsible for nosocomial pneumonia. We sequenced the genome from the L. anisa strain Linanisette, which was recovered from a clinical sample using an amoebal coculture procedure but not with standard culture methods.
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Genome Sequence of Legionella tunisiensis Strain LegMT, a New Legionella Species Isolated from Hypersaline Lake Water
Journal of bacteriology, 2012Co-Authors: Isabelle Pagnier, Mondher Boughalmi, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella tunisiensis is a gammaproteobacterium from the class Legionellaceae, growing in amoebae. We sequenced the genome from strain LegMT. It is composed of 3,508,121 bp and contains 4,747 protein-coding genes and 38 RNA genes, including 3 rRNA genes.
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Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Angélique Campocasso, Mondher Boughalmi, Didier Raoult, Ghislain Fournous, Bernard La ScolaAbstract:Two isolates of intra-amoeba-growing bacteria, LegAT ( = DSM 24804T = CSUR P146T) and LegMT ( = DSM 24805T = CSUR P145T), were characterized on the basis of microscopic appearance, staining characteristics, axenic growth at different temperatures and the sequences of the mip, rpoB, 16S rRNA and rnpb genes, as well as the 23S–5S region. Phylogenetic analysis showed that these two isolates lay within the radius of the family Legionellaceae . Furthermore, the analysis of these genes yielded congruent data that indicated that, although strain LegMT clusters specifically with Legionella feeleii ATCC 35072T and LegAT clusters with Legionella nautarum ATCC 49596T, the divergence observed between these species was greater than that observed between other members of the family. Taken together, these results support the proposal that these two isolates represent novel members of the genus Legionella , and we propose to name them Legionella tunisiensis sp. nov. for LegMT ( = DSM 24805T = CSUR P145T) and Legionella massiliensis sp. nov. for LegAT ( = DSM 24804T = CSUR P146T).
Didier Raoult - One of the best experts on this subject based on the ideXlab platform.
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Legionella saoudiensis sp nov., isolated from a sewage water sample
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1(T), was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1(T) was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1(T) belongs to the genus Legionella in the family Legionellaceae. Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6(T) (98.6 %) and Legionella lytica L2(T) (98.5 %). The mip gene sequence of strain LS-1(T) showed 94% sequence similarity with that of L. lytica L2(T) and 93% similarity with that of L. rowbothamii LLAP-6(T). Strain LS-1(T) grew optimally at a temperature of 32 degrees C on a buffered charcoal yeast extract (BCYE) agar plate in a 5% CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1(T) represents a novel species of the genus Legionella, for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1(T) (= DSM 101682(T) = CSUR P2101(T)).
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Legionella saoudiensis sp. nov., isolated from a sewage water sample.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1T, was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1T was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1T belongs to the genus Legionella in the family Legionellaceae . Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6T (98.6 %) and Legionella lytica L2T (98.5 %). The mip gene sequence of strain LS-1T showed 94 % sequence similarity with that of L. lytica L2T and 93 % similarity with that of L. rowbothamii LLAP-6T. Strain LS-1T grew optimally at a temperature of 32 °C on a buffered charcoal yeast extract (BCYE) agar plate in a 5 % CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1T represents a novel species of the genus Legionella , for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1T (=DSM 101682T=CSUR P2101T).
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Genome Sequence of Legionella anisa, Isolated from a Respiratory Sample, Using an Amoebal Coculture Procedure.
Genome announcements, 2014Co-Authors: Isabelle Pagnier, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella anisa is a gammaproteobacterium from the class Legionellaceae, which is responsible for nosocomial pneumonia. We sequenced the genome from the L. anisa strain Linanisette, which was recovered from a clinical sample using an amoebal coculture procedure but not with standard culture methods.
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Genome Sequence of Legionella tunisiensis Strain LegMT, a New Legionella Species Isolated from Hypersaline Lake Water
Journal of bacteriology, 2012Co-Authors: Isabelle Pagnier, Mondher Boughalmi, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella tunisiensis is a gammaproteobacterium from the class Legionellaceae, growing in amoebae. We sequenced the genome from strain LegMT. It is composed of 3,508,121 bp and contains 4,747 protein-coding genes and 38 RNA genes, including 3 rRNA genes.
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Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Angélique Campocasso, Mondher Boughalmi, Didier Raoult, Ghislain Fournous, Bernard La ScolaAbstract:Two isolates of intra-amoeba-growing bacteria, LegAT ( = DSM 24804T = CSUR P146T) and LegMT ( = DSM 24805T = CSUR P145T), were characterized on the basis of microscopic appearance, staining characteristics, axenic growth at different temperatures and the sequences of the mip, rpoB, 16S rRNA and rnpb genes, as well as the 23S–5S region. Phylogenetic analysis showed that these two isolates lay within the radius of the family Legionellaceae . Furthermore, the analysis of these genes yielded congruent data that indicated that, although strain LegMT clusters specifically with Legionella feeleii ATCC 35072T and LegAT clusters with Legionella nautarum ATCC 49596T, the divergence observed between these species was greater than that observed between other members of the family. Taken together, these results support the proposal that these two isolates represent novel members of the genus Legionella , and we propose to name them Legionella tunisiensis sp. nov. for LegMT ( = DSM 24805T = CSUR P145T) and Legionella massiliensis sp. nov. for LegAT ( = DSM 24804T = CSUR P146T).
Isabelle Pagnier - One of the best experts on this subject based on the ideXlab platform.
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Legionella saoudiensis sp nov., isolated from a sewage water sample
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1(T), was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1(T) was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1(T) belongs to the genus Legionella in the family Legionellaceae. Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6(T) (98.6 %) and Legionella lytica L2(T) (98.5 %). The mip gene sequence of strain LS-1(T) showed 94% sequence similarity with that of L. lytica L2(T) and 93% similarity with that of L. rowbothamii LLAP-6(T). Strain LS-1(T) grew optimally at a temperature of 32 degrees C on a buffered charcoal yeast extract (BCYE) agar plate in a 5% CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1(T) represents a novel species of the genus Legionella, for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1(T) (= DSM 101682(T) = CSUR P2101(T)).
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Legionella saoudiensis sp. nov., isolated from a sewage water sample.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1T, was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1T was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1T belongs to the genus Legionella in the family Legionellaceae . Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6T (98.6 %) and Legionella lytica L2T (98.5 %). The mip gene sequence of strain LS-1T showed 94 % sequence similarity with that of L. lytica L2T and 93 % similarity with that of L. rowbothamii LLAP-6T. Strain LS-1T grew optimally at a temperature of 32 °C on a buffered charcoal yeast extract (BCYE) agar plate in a 5 % CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1T represents a novel species of the genus Legionella , for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1T (=DSM 101682T=CSUR P2101T).
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Genome Sequence of Legionella anisa, Isolated from a Respiratory Sample, Using an Amoebal Coculture Procedure.
Genome announcements, 2014Co-Authors: Isabelle Pagnier, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella anisa is a gammaproteobacterium from the class Legionellaceae, which is responsible for nosocomial pneumonia. We sequenced the genome from the L. anisa strain Linanisette, which was recovered from a clinical sample using an amoebal coculture procedure but not with standard culture methods.
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Genome Sequence of Legionella tunisiensis Strain LegMT, a New Legionella Species Isolated from Hypersaline Lake Water
Journal of bacteriology, 2012Co-Authors: Isabelle Pagnier, Mondher Boughalmi, Olivier Croce, Catherine Robert, Didier Raoult, Bernard La ScolaAbstract:ABSTRACT Legionella tunisiensis is a gammaproteobacterium from the class Legionellaceae, growing in amoebae. We sequenced the genome from strain LegMT. It is composed of 3,508,121 bp and contains 4,747 protein-coding genes and 38 RNA genes, including 3 rRNA genes.
Jette M Bangsborg - One of the best experts on this subject based on the ideXlab platform.
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Update on Rifampin Resistance in the Legionellaceae
Antimicrobial agents and chemotherapy, 2001Co-Authors: Annerose Serr, Barbara F. Koenig, Markus Heep, Kim G. Nielsen, Jette M BangsborgAbstract:A recent article described rpoB mutations in 18 rifampin-resistant variants of Legionella spp. ([4][1]). One of these 18 strains, isolate 8749, an in vitro-generated high-level rifampin-resistant (MIC ≥ 256 μg/ml) variant of K26, a susceptible clinical Legionella bozemanii isolate, was shown to
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Sequencing of the rpoB Gene in Legionella pneumophila and Characterization of Mutations Associated with Rifampin Resistance in the Legionellaceae
Antimicrobial agents and chemotherapy, 2000Co-Authors: Kaare Magne Nielsen, Niels Høiby, Peter Hindersson, Jette M BangsborgAbstract:Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis. Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis, and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae. Since the RNA polymerase genes of this genus have never been characterized, the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking. A 4,647-bp DNA sequence that contained the open reading frame (ORF) of the rpoB gene (4,104 bp) and an ORF of 384 bp representing part of the rpoC gene was obtained. A 316-bp DNA fragment in the center of the L. pneumophila rpoB gene, corresponding to a previously described site for mutations leading to rifampin resistance in M. tuberculosis, was sequenced from 18 rifampin-resistant Legionella isolates representing four species (L. bozemanii, L. longbeachae, L. micdadei, and L. pneumophila), and the sequences were compared to the sequences of the fragments from the parent (rifampin-sensitive) strains. Six single-base mutations which led to amino acid substitutions at five different positions were identified. A single strain did not contain any mutations in the 316-bp fragment. This study represents the characterization of a hitherto undescribed resistance mechanism within the family Legionellaceae.
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Restriction fragment length polymorphism of rRNA genes for molecular typing of members of the family Legionellaceae.
Journal of clinical microbiology, 1995Co-Authors: Jette M Bangsborg, P Gerner-smidt, H Colding, Nils-erik Fiehn, Brita Bruun, Niels HøibyAbstract:Typing of Legionella pneumophila remains important in the investigation of outbreaks of Legionnaires' disease and in the control of organisms contaminating hospital water. We found that the discriminatory power of a nonradioactive ribotyping method could be improved by combining results obtained with four restriction enzymes (HindIII, NciI, ClaI, and PstI). Fifty-eight clinical and environmental L. pneumophila strains including geographically unrelated as well as epidemiologically connected isolates were investigated. Epidemiologically related strains had the same ribotypes independent of the combinations of enzymes used. Some strains belonging to the same serogroup were assigned to different ribotypes, and some ribotypes contained members of different serogroups, indicating, as others have found, that serogroup and genotype are not always related. The discriminatory power of the method was estimated by calculating an index of discrimination (ID) for individual enzymes and combinations thereof. The combined result with all four enzymes was highly discriminatory (ID = 0.97), but results for three enzymes also yielded ID values acceptable for epidemiological purposes. In addition, the testing of 27 type strains and 6 clinical isolates representing Legionella species other than L. pneumophila indicated that ribotyping might be of value for species identification within this genus, as previously suggested.
Leena H. Bajrai - One of the best experts on this subject based on the ideXlab platform.
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Legionella saoudiensis sp nov., isolated from a sewage water sample
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1(T), was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1(T) was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1(T) belongs to the genus Legionella in the family Legionellaceae. Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6(T) (98.6 %) and Legionella lytica L2(T) (98.5 %). The mip gene sequence of strain LS-1(T) showed 94% sequence similarity with that of L. lytica L2(T) and 93% similarity with that of L. rowbothamii LLAP-6(T). Strain LS-1(T) grew optimally at a temperature of 32 degrees C on a buffered charcoal yeast extract (BCYE) agar plate in a 5% CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1(T) represents a novel species of the genus Legionella, for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1(T) (= DSM 101682(T) = CSUR P2101(T)).
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Legionella saoudiensis sp. nov., isolated from a sewage water sample.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Leena H. Bajrai, Didier Raoult, Bernard La Scola, Esam I. Azhar, Muhammad Yasir, Priscilla Jardot, Lina Barrassi, Isabelle PagnierAbstract:A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1T, was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1T was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1T belongs to the genus Legionella in the family Legionellaceae . Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6T (98.6 %) and Legionella lytica L2T (98.5 %). The mip gene sequence of strain LS-1T showed 94 % sequence similarity with that of L. lytica L2T and 93 % similarity with that of L. rowbothamii LLAP-6T. Strain LS-1T grew optimally at a temperature of 32 °C on a buffered charcoal yeast extract (BCYE) agar plate in a 5 % CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1T represents a novel species of the genus Legionella , for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1T (=DSM 101682T=CSUR P2101T).