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Xuening Wang - One of the best experts on this subject based on the ideXlab platform.

  • Linear Epitope landscape of the sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Cell Reports, 2021
    Co-Authors: Qing Lei, Feng Wang, Wei Hong, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang
    Abstract:

    To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a Linear Epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in Linear Epitopes, i.e., C-terminal domain (CTD) and a region close to the S2' cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks Linear Epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain Linear-Epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Social Science Research Network, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S2’ cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level. Funding: This work was partially supported by National Key Research and Development Program of China Grant (No. 2016YFA0500600), Science and Technology Commission of Shanghai Municipality (No. 19441911900), Interdisciplinary Program of Shanghai Jiao Tong University (No. YG2020YQ10), National Natural Science Foundation of China (No. 31970130, 31600672, 31670831, and 31370813). Conflict of Interest: The authors declare no competing interest.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    medRxiv, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S29 cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level.

Qing Lei - One of the best experts on this subject based on the ideXlab platform.

  • Linear Epitope landscape of the sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Cell Reports, 2021
    Co-Authors: Qing Lei, Feng Wang, Wei Hong, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang
    Abstract:

    To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a Linear Epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in Linear Epitopes, i.e., C-terminal domain (CTD) and a region close to the S2' cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks Linear Epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain Linear-Epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Social Science Research Network, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S2’ cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level. Funding: This work was partially supported by National Key Research and Development Program of China Grant (No. 2016YFA0500600), Science and Technology Commission of Shanghai Municipality (No. 19441911900), Interdisciplinary Program of Shanghai Jiao Tong University (No. YG2020YQ10), National Natural Science Foundation of China (No. 31970130, 31600672, 31670831, and 31370813). Conflict of Interest: The authors declare no competing interest.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    medRxiv, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S29 cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level.

Ningshao Xia - One of the best experts on this subject based on the ideXlab platform.

  • rabbit monoclonal antibody specifically recognizing a Linear Epitope in the rbd of sars cov 2 spike protein
    Vaccine, 2021
    Co-Authors: Junping Hong, Qian Wang, Junyu Chen, Xijing Wang, Yingbin Wang, Yixin Chen, Ningshao Xia
    Abstract:

    To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare Linear Epitope. So far, no Linear Epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes Linear Epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved Linear Epitope within twelve amino acids 480CNGVEGFNCYFP491 on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2.

  • a highly conserved Epitope vaccine candidate against varicella zoster virus induces neutralizing antibodies in mice
    Vaccine, 2016
    Co-Authors: Rui Zhu, Jian Liu, Chunye Chen, Wei Wang, Qinjian Zhao, Hua Zhu, Tong Cheng, Ningshao Xia
    Abstract:

    Varicella-zoster virus (VZV) is a highly infectious agent of varicella and herpes zoster (HZ). Vaccination is by far the most effective way to prevent these diseases. More safe, stable and efficient vaccines, such as Epitope-based vaccines, now have been increasingly investigated by many researchers. However, only a few VZV neutralizing Epitopes have been identified to date. We have previously identified a Linear Epitope between amino acid residues 121 and 135 of gE. In this study, we validated that this Epitope is highly conserved amongst different VZV strains that covered five existing phylogenetic clades with an identity of 100%. We evaluated the immunogenicity of the recombinant hepatitis B virus core (HBc) virus-like particles (VLPs) which included amino acids (121-135). VZV-gE-specific antibodies were detected in immunized mouse serum using ELISA. The anti-peptide antiserum positively detected VZV via Western blot and immunofluorescent staining assays. More importantly, these peptides could neutralize VZV, indicating that these peptides represented neutralizing Epitopes. These findings have important implications for the development of Epitope-based protective VZV vaccines.

  • a novel Linear neutralizing Epitope of hepatitis e virus
    Vaccine, 2015
    Co-Authors: Zimin Tang, Ming Tang, Min Zhao, Guiping Wen, Fan Yang, Wei Cai, Siling Wang, Zizheng Zheng, Ningshao Xia
    Abstract:

    Hepatitis E virus (HEV) is a serious public health problem that causes acute hepatitis in humans and is primarily transmitted through fecal and oral routes. The major anti-HEV antibody responses are against conformational Epitopes located in a.a. 459–606 of HEV pORF2. All reported neutralization Epitopes are present on the dimer domain constructed by this peptide. While looking for a neutralizing monoclonal antibody (MAb)-recognized Linear Epitope, we found a novel neutralizing Linear Epitope (L2) located in a.a. 423–437 of pORF2. Moreover, Epitope L2 is proved non-immunodominant in the HEV-infection process. Using the hepatitis B virus core protein (HBc) as a carrier to display this novel Linear Epitope, we show herein that this Epitope could induce a neutralizing antibody response against HEV in mice and could protect rhesus monkeys from HEV infection. Collectively, our results showed a novel non-immunodominant Linear neutralizing Epitope of hepatitis E virus, which provided additional insight of HEV vaccine.

Hewei Jiang - One of the best experts on this subject based on the ideXlab platform.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Social Science Research Network, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S2’ cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level. Funding: This work was partially supported by National Key Research and Development Program of China Grant (No. 2016YFA0500600), Science and Technology Commission of Shanghai Municipality (No. 19441911900), Interdisciplinary Program of Shanghai Jiao Tong University (No. YG2020YQ10), National Natural Science Foundation of China (No. 31970130, 31600672, 31670831, and 31370813). Conflict of Interest: The authors declare no competing interest.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    medRxiv, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S29 cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level.

Danyun Lai - One of the best experts on this subject based on the ideXlab platform.

  • sars cov 2 spike Linear Epitope scanning via a peptide microarray through sera profiling
    STAR protocols, 2021
    Co-Authors: Danyun Lai, Shengce Tao
    Abstract:

    Host humoral immunological response plays an essential role in protection against pathogens. Identification- of B cell Epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 Spike Linear Epitopes, we developed a protocol of profiling sera from COVID-19 patients via a peptide microarray designed according to Spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high-throughput and the cost is acceptable while it needs specialized microarray facilities.

  • Linear Epitope landscape of the sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Cell Reports, 2021
    Co-Authors: Qing Lei, Feng Wang, Wei Hong, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang
    Abstract:

    To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a Linear Epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in Linear Epitopes, i.e., C-terminal domain (CTD) and a region close to the S2' cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks Linear Epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain Linear-Epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    Social Science Research Network, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S2’ cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level. Funding: This work was partially supported by National Key Research and Development Program of China Grant (No. 2016YFA0500600), Science and Technology Commission of Shanghai Municipality (No. 19441911900), Interdisciplinary Program of Shanghai Jiao Tong University (No. YG2020YQ10), National Natural Science Foundation of China (No. 31970130, 31600672, 31670831, and 31370813). Conflict of Interest: The authors declare no competing interest.

  • Linear Epitope landscape of sars cov 2 spike protein constructed from 1 051 covid 19 patients
    medRxiv, 2020
    Co-Authors: Qing Lei, Feng Wang, Danyun Lai, Hongyan Hou, Bo Zhang, Hong Chen, Junbiao Xue, Yunxiao Zheng, Xuening Wang, Hewei Jiang
    Abstract:

    Neutralization antibodies and vaccines for treating COVID-19 are desperately needed. For precise development of antibodies and vaccines, the key is to understand which part of SARS-CoV-2 Spike protein is highly immunogenic on a systematic way. We generate a Linear Epitope landscape of Spike protein by analyzing serum IgG response of 1,051 COVID-19 patients with a peptide microarray. We reveal two regions that rich of Linear Epitopes, i.e., CTD and a region close to the S29 cleavage site and fusion peptide. Unexpectedly, we find RBD is lack of Linear Epitope. Besides 3 moderate immunogenic peptides from RBD, 16 highly immunogenic peptides from other regions of Spike protein are determined. These peptides could serve as the base for precise development of antibodies and vaccines for COVID-19 on a systematic level.