The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Digang Zeng - One of the best experts on this subject based on the ideXlab platform.
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the penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin i
Fish & Shellfish Immunology, 2019Co-Authors: Xiuli Chen, Digang Zeng, Chunling Yang, Min Peng, Qiong Yang, Weilin Zhu, Qingyun Liu, Hui Wang, Yong Lin, Yongzhen ZhaoAbstract:The Penaeus stylirostris densovirus (PstDNV) (also known as infectious hypodermal and hematopoietic necrosis virus, IHHNV), a very small DNA virus, is a major shrimp pathogen. The PstDNV genome encodes only two nonstructural proteins and one capsid protein. This virus is thus an ideal, simple model for the investigation of virus-host interactions. To explore the role of the PstDNV capsid in viral infections, a yeast two-hybrid (Y2H) cDNA library was constructed based on Pacific white shrimp, Litopenaeus vannamei mRNA. The Y2H library was then screened, using the PstDNV capsid protein as bait. We identified a host protein that interacted strongly with the PstDNV capsid as L. vannamei troponin I (LvTnI). An in vitro co-immunoprecipitation experiment further supported this interaction. In addition, an in vivo neutralization experiment showed that the vaccination with anti-LvTnI significantly reduced PstDNV copies in PstDNV-challenged shrimp, indicating that the interaction between the PstDNV capsid and cellular LvTnI is essential for PstDNV infection. This result has important implications for our understanding of the mechanisms by which PstDNV infects shrimp.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
PLoS ONE, 2013Co-Authors: Digang Zeng, Chen Xiuli, Daxiang Xie, Zhao Yongzhen, Chunling Yang, Min Peng, Yang Qiong, Zhenping LiaoAbstract:Background The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
2013Co-Authors: Digang Zeng, Daxiang Xie, Chunling Yang, Min Peng, Xiuli Chen, Yongzhen Zhao, Qiong Yang, Zhenping LiaoAbstract:BackgroundThe Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology. Methodology/Principal FindingsWe obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value
Zhenping Liao - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
PLoS ONE, 2013Co-Authors: Digang Zeng, Chen Xiuli, Daxiang Xie, Zhao Yongzhen, Chunling Yang, Min Peng, Yang Qiong, Zhenping LiaoAbstract:Background The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
2013Co-Authors: Digang Zeng, Daxiang Xie, Chunling Yang, Min Peng, Xiuli Chen, Yongzhen Zhao, Qiong Yang, Zhenping LiaoAbstract:BackgroundThe Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology. Methodology/Principal FindingsWe obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value
Chunling Yang - One of the best experts on this subject based on the ideXlab platform.
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the penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin i
Fish & Shellfish Immunology, 2019Co-Authors: Xiuli Chen, Digang Zeng, Chunling Yang, Min Peng, Qiong Yang, Weilin Zhu, Qingyun Liu, Hui Wang, Yong Lin, Yongzhen ZhaoAbstract:The Penaeus stylirostris densovirus (PstDNV) (also known as infectious hypodermal and hematopoietic necrosis virus, IHHNV), a very small DNA virus, is a major shrimp pathogen. The PstDNV genome encodes only two nonstructural proteins and one capsid protein. This virus is thus an ideal, simple model for the investigation of virus-host interactions. To explore the role of the PstDNV capsid in viral infections, a yeast two-hybrid (Y2H) cDNA library was constructed based on Pacific white shrimp, Litopenaeus vannamei mRNA. The Y2H library was then screened, using the PstDNV capsid protein as bait. We identified a host protein that interacted strongly with the PstDNV capsid as L. vannamei troponin I (LvTnI). An in vitro co-immunoprecipitation experiment further supported this interaction. In addition, an in vivo neutralization experiment showed that the vaccination with anti-LvTnI significantly reduced PstDNV copies in PstDNV-challenged shrimp, indicating that the interaction between the PstDNV capsid and cellular LvTnI is essential for PstDNV infection. This result has important implications for our understanding of the mechanisms by which PstDNV infects shrimp.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
PLoS ONE, 2013Co-Authors: Digang Zeng, Chen Xiuli, Daxiang Xie, Zhao Yongzhen, Chunling Yang, Min Peng, Yang Qiong, Zhenping LiaoAbstract:Background The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
2013Co-Authors: Digang Zeng, Daxiang Xie, Chunling Yang, Min Peng, Xiuli Chen, Yongzhen Zhao, Qiong Yang, Zhenping LiaoAbstract:BackgroundThe Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology. Methodology/Principal FindingsWe obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value
Min Peng - One of the best experts on this subject based on the ideXlab platform.
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the penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin i
Fish & Shellfish Immunology, 2019Co-Authors: Xiuli Chen, Digang Zeng, Chunling Yang, Min Peng, Qiong Yang, Weilin Zhu, Qingyun Liu, Hui Wang, Yong Lin, Yongzhen ZhaoAbstract:The Penaeus stylirostris densovirus (PstDNV) (also known as infectious hypodermal and hematopoietic necrosis virus, IHHNV), a very small DNA virus, is a major shrimp pathogen. The PstDNV genome encodes only two nonstructural proteins and one capsid protein. This virus is thus an ideal, simple model for the investigation of virus-host interactions. To explore the role of the PstDNV capsid in viral infections, a yeast two-hybrid (Y2H) cDNA library was constructed based on Pacific white shrimp, Litopenaeus vannamei mRNA. The Y2H library was then screened, using the PstDNV capsid protein as bait. We identified a host protein that interacted strongly with the PstDNV capsid as L. vannamei troponin I (LvTnI). An in vitro co-immunoprecipitation experiment further supported this interaction. In addition, an in vivo neutralization experiment showed that the vaccination with anti-LvTnI significantly reduced PstDNV copies in PstDNV-challenged shrimp, indicating that the interaction between the PstDNV capsid and cellular LvTnI is essential for PstDNV infection. This result has important implications for our understanding of the mechanisms by which PstDNV infects shrimp.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
PLoS ONE, 2013Co-Authors: Digang Zeng, Chen Xiuli, Daxiang Xie, Zhao Yongzhen, Chunling Yang, Min Peng, Yang Qiong, Zhenping LiaoAbstract:Background The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.
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Transcriptome Analysis of Pacific White Shrimp (Litopenaeus vannamei) Hepatopancreas in Response to Taura Syndrome Virus (TSV) Experimental Infection
2013Co-Authors: Digang Zeng, Daxiang Xie, Chunling Yang, Min Peng, Xiuli Chen, Yongzhen Zhao, Qiong Yang, Zhenping LiaoAbstract:BackgroundThe Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology. Methodology/Principal FindingsWe obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value
Jianhai Xiang - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of shrimp hemocyte iridescent virus shiv isolated from white leg shrimp Litopenaeus vannamei
Archives of Virology, 2018Co-Authors: Mengmeng Chen, Chen Li, Qingli Zhang, Ruoyu Wang, Xuan Dong, Bing Yang, Jianhai XiangAbstract:Infection with shrimp hemocyte iridescent virus (SHIV), a new virus of the family Iridoviridae isolated in China, results in a high mortality rate in white leg shrimp (Litopenaeus vannamei). The complete genome sequence of SHIV was determined and analyzed in this study. The genomic DNA was 165,809 bp long with 34.6% G+C content and 170 open reading frames (ORFs). Dotplot analysis showed that the longest repetitive region was 320 bp in length, including 11 repetitions of an 18-bp sequence and 3.1 repetitions of a 39-bp sequence. Two phylogenetic trees were constructed based on 27 or 16 concatenated sequences of proteins encoded by genes that are conserved between SHIV homologous and other iridescent viruses. The results of this study, suggest that SHIV should be considered a member of the proposed new genus “Xiairidovirus”.
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comparative transcriptomic characterization of the early development in pacific white shrimp Litopenaeus vannamei
PLOS ONE, 2014Co-Authors: Jiankai Wei, Xiaojun Zhang, Hao Huang, Jianhai XiangAbstract:Penaeid shrimp has a distinctive metamorphosis stage during early development. Although morphological and biochemical studies about this ontogeny have been developed for decades, researches on gene expression level are still scarce. In this study, we have investigated the transcriptomes of five continuous developmental stages in Pacific white shrimp (Litopenaeus vannamei) with high throughput Illumina sequencing technology. The reads were assembled and clustered into 66,815 unigenes, of which 32,398 have putative homologues in nr database, 14,981 have been classified into diverse functional categories by Gene Ontology (GO) annotation and 26,257 have been associated with 255 pathways by KEGG pathway mapping. Meanwhile, the differentially expressed genes (DEGs) between adjacent developmental stages were identified and gene expression patterns were clustered. By GO term enrichment analysis, KEGG pathway enrichment analysis and functional gene profiling, the physiological changes during shrimp metamorphosis could be better understood, especially histogenesis, diet transition, muscle development and exoskeleton reconstruction. In conclusion, this is the first study that characterized the integrated transcriptomic profiles during early development of penaeid shrimp, and these findings will serve as significant references for shrimp developmental biology and aquaculture research.
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immune response of Litopenaeus vannamei after infection with vibrio harveyi
Aquaculture, 2013Co-Authors: Haihong Huang, Jianhai Xiang, Xiaolin Liu, Ping WangAbstract:To investigate the immune response of Litopenaeus vannamei to Vibrio infection, two L. vannamei groups, Vibrio-resistant shrimp and normal shrimp, were injected with a pathogenic strain of Vibrio harveyi at a dose of 1.0 x 10(5) cfu shrimp (1), and then their Vibrio density, total hemocyte count (THC), hemocyanin concentration, and the activity of phenoloxidase (PO), superoxide dismutase (SOD) and anti microbial peptides (AMPs) were determined. Results showed that resistant shrimp and normal shrimp cleared the V. harveyi infecting hemolymph during 3 h and 12 h after injection, respectively. The level of THC, hemocyanin concentration, and the activity of PO, SOD and AMPs in resistant shrimp was significantly higher at the beginning, and earlier decreased and recovered after injection than that in normal shrimp, respectively. In conclusion, hemocyte, hemocyanin, PO, SOD and AMPs were important for the response of both resistant and normal L. vannamei after V. harveyi injection, and the immune response in resistant shrimp was more efficient than that of normal shrimp. (C) 2013 Elsevier B.V. All rights reserved.
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structure and partial protein profiles of the peritrophic membrane pm from the gut of the shrimp Litopenaeus vannamei
Fish & Shellfish Immunology, 2012Co-Authors: Liyan Wang, Bing Wang, Jianhai XiangAbstract:Peritrophic membrane (PM) is a non-cellular structure surrounding the food bolus in invertebrate's midgut. In this study, the shrimp Litopenaeus vannamei was found continuously secreting a tube-like PM enclosing the fecal pellets. The PM structure was membranous in this penaeid shrimp which was similar to that in Sicyonia ingentis studied and was primarily composed of chitin and proteins. Chitin was detected along the whole PM. By using the approach of gel electrophoresis and mass spectrometry of tryptic peptides, the most extracted proteins from the shrimp PMs were identified mainly including digestion-related, immune-related, antioxidant proteins and proteins related to PM structure. This suggests that PM may participate in modulating its permeability and immobilizating the digestive enzymes, actively protect the gut from pathogen contact, and play an important role in the gut immune system.
