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Jens Stougaard - One of the best experts on this subject based on the ideXlab platform.
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Nitrate inhibits nodule organogenesis through inhibition of cytokinin biosynthesis in Lotus Japonicus
2020Co-Authors: Jie-shun Lin, Jens Stougaard, Yuda Purwana Roswanjaya, Wouter Kohlen, Dugald ReidAbstract:Abstract Legumes balance nitrogen acquisition from soil nitrate with symbiotic nitrogen fixation. Nitrogen fixation requires establishment of a new organ, which is a cytokinin dependent developmental process in the root. We found cytokinin biosynthesis is a central integrator, balancing nitrate signalling with symbiotic acquired nitrogen. Low nitrate conditions provide a permissive state for induction of cytokinin by symbiotic signalling and thus nodule development. In contrast, high nitrate is inhibitory to cytokinin accumulation and nodule establishment in the root zone susceptible to nodule formation. This reduction of symbiotic cytokinin accumulation was further exacerbated in cytokinin biosynthesis mutants, which display hypersensitivity to nitrate inhibition of nodule development, maturation and nitrogen fixation. Consistent with this, cytokinin application can rescue nodulation and nitrogen fixation of biosynthesis mutants in a concentration dependent manner. These inhibitory impacts of nitrate on symbiosis occur in a Nlp1 and Nlp4 dependent manner and contrast with the positive influence of nitrate on cytokinin biosynthesis that occurs in non-symbiotic species. Altogether this shows that legumes, as exemplified by Lotus Japonicus, have evolved a different cytokinin response to nitrate compared to non-legumes. One sentence summary Cytokinin biosynthesis is suppressed by nitrate in Lotus Japonicus, providing a mechanism for nitrate inhibition of symbiotic nodule organogenesis.
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The deubiquitinating enzyme AMSH1 is required for rhizobial infection and nodule organogenesis in Lotus Japonicus
The Plant journal : for cell and molecular biology, 2015Co-Authors: Anna Małolepszy, Jens Stougaard, Niels Sandal, Euan K. James, Dorian Fabian Urbański, Erika Isono, Stig Uggerhøj AndersenAbstract:Legume-rhizobium symbiosis contributes large quantities of fixed nitrogen to both agricultural and natural ecosystems. This global impact and the selective interaction between rhizobia and legumes culminating in development of functional root nodules have prompted detailed studies of the underlying mechanisms. We performed a screen for aberrant nodulation phenotypes using the Lotus Japonicus LORE1 insertion mutant collection. Here, we describe the identification of amsh1 mutants that only develop small nodule primordia and display stunted shoot growth, and show that the aberrant nodulation phenotype caused by LORE1 insertions in the Amsh1 gene may be separated from the shoot phenotype. In amsh1 mutants, rhizobia initially became entrapped in infection threads with thickened cells walls. Some rhizobia were released into plant cells much later than observed for the wild-type; however, no typical symbiosome structures were formed. Furthermore, cytokinin treatment only very weakly induced nodule organogenesis in amsh1 mutants, suggesting that AMSH1 function is required downstream of cytokinin signaling. Biochemical analysis showed that AMSH1 is an active deubiquitinating enzyme, and that AMSH1 specifically cleaves K63-linked ubiquitin chains. Post-translational ubiquitination and deubiquitination processes involving the AMSH1 deubiquitinating enzyme are thus involved in both infection and organogenesis in Lotus Japonicus.
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Background and History of the Lotus Japonicus Model Legume System
Compendium of Plant Genomes, 2014Co-Authors: Jens StougaardAbstract:The combination of favourable biological features, stable transformation procedures, application of genetics and genome-based global approaches has established Lotus Japonicus as a model legume and provided a platform for addressing important biological questions often, but not exclusively, focusing on endosymbiosis. Several important discoveries have been made, and the Lotus community has contributed novel results, promoting our understanding of plant biology as well as our understanding of properties and characteristics typical for plants belonging to the legume family. Progress has been fast since L. Japonicus was first promoted as a model plant yet there are many challenges for the coming years. This introductory chapter will set the stage for some of these challenges, while possibilities and challenges emerging from specific research projects will be addressed in the chapters that follow.
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High-Throughput and Targeted Genotyping of Lotus Japonicus LORE1 Insertion Mutants
Methods in molecular biology (Clifton N.J.), 2013Co-Authors: Dorian Fabian Urbański, Jens Stougaard, Anna Małolepszy, Stig Uggerhøj AndersenAbstract:The Lotus Retrotransposon 1 (LORE1) is used for genome-wide mutagenesis of the model legume Lotus Japonicus. Characterization of the LORE1 insertion sites in individual mutant lines is critical for development and use of the resource. Here we present guidelines for use of the LORE1 reverse genetics resource and provide detailed protocols for insertion site identification and validation. For high-throughput identification of insertions in up to 9,216 pooled lines, the FSTpoolit protocol takes advantage of Splinkerette adapters, molecular barcoding, 2D pooling, Illumina sequencing, and automated data analysis using the freely available FSTpoolit software. Complementing the high-throughput approach, we describe a simplified sequence-specific amplification polymorphism (SSAP) protocol well suited for quick identification of insertion sites in a limited number of lines. Both the FSTpoolit and simplified SSAP protocols are generally applicable to insertion site identification in any insertional mutagenesis setup.
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Lotus Japonicus : A Model Plant for the Legume Family
Brenner's Encyclopedia of Genetics, 2013Co-Authors: Jens StougaardAbstract:Legumes have a unique ability to enter into nitrogen fixing symbiosis with soil bacteria collectively called rhizobia and phosphate scavenging symbiosis with mycorrhizal fungi. In this short article, the biological and methodological features qualifying Lotus Japonicus as a model legume are presented together with the genetic and genomic resources supporting the research activities on symbiosis, seed development, and many other traits. Summarizing a decade of gene discoveries, the new understanding of the genetic program and pathways enabling legumes to develop nitrogen fixing root nodules in symbiosis with rhizobial bacteria is highlighted. The tools established for translation of insight from the Lotus model system to the less-studied cultivated legumes are described. Finally, the proteomic approaches developed to characterize and understand the development of protein- and oil-rich seeds that is a key feature of legumes are described.
Satoshi Tabata - One of the best experts on this subject based on the ideXlab platform.
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Lotus Japonicus ARPC1 Is Required for Rhizobial Infection
Plant physiology, 2012Co-Authors: Shakhawat Hossain, Shusei Sato, Satoshi Tabata, Jens Stougaard, Lene H. Madsen, Anna Jurkiewicz, Euan K. James, Jinqiu Liao, Loretta Ross, Krzysztof SzczyglowskiAbstract:Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus Japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. Japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. Japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.
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Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus Japonicus
Molecular plant-microbe interactions : MPMI, 2008Co-Authors: Takaki Maekawa, Shusei Sato, Satoshi Tabata, Yoshikatsu Murooka, Mitsumasa Kusakabe, Yoshikazu Shimoda, Makoto HayashiAbstract:In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus Japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. Japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. Japonicus.
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Lotus Japonicus as a platform for legume research.
Current opinion in plant biology, 2006Co-Authors: Shusei Sato, Satoshi TabataAbstract:The major role of 'model plants' is to provide knowledge and technologies obtained in related systems to researchers studying crop plants. Lotus Japonicus was chosen as a model system first for legume genetics and then for legume genomics. A large number of L. Japonicus mutants that have alterations in legume-specific phenomena have been generated and phenotypically characterized, and genomics has drastically accelerated the molecular characterization of these mutants. Substantial resources of information and experimental materials, including genomic and cDNA sequences, corresponding DNA libraries and high-density linkage maps demonstrate that L. Japonicus is an excellent model system. Transfer of knowledge from L. Japonicus to other legumes, especially crop legumes, is a matter for urgent consideration.
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Functional and structural analysis of genes encoding oxidosqualene cyclases of Lotus Japonicus
Plant Science, 2006Co-Authors: Satoru Sawai, Tamotsu Shindo, Takakazu Kaneko, Shin Ichi Ayabe, Shusei Sato, Satoshi Tabata, Toshio AokiAbstract:Abstract The structures of cyclic triterpenoids of higher plants, whose skeletons are constructed by oxidosqualene cyclases (OSCs), are remarkably diverse in contrast to the limited variation in sterol/triterpenoid structures of other organisms. We comprehensively analyzed the functions and structures of OSC genes of a model legume, Lotus Japonicus , and compared them with those of Arabidopsis thaliana in order to elucidate the diversification process of plant cyclic triterpenoids. Eight OSC genes ( OSC1 – OSC8 ) formed two sets of clusters in chromosomes 2 and 3 of L. Japonicus . OSC1 and OSC8 corresponded to the previously reported LjAMY1 and LjAMY2, respectively [I. Iturbe-Ormaetxe, K. Haralampidis, K. Papadopoulou, A.E. Osbourn, Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus Japonicus , Plant Mol. Biol. 51 (2003) 731–743]. OSC1 (the full-length version of LjAMY1), OSC3 and OSC5 were identified as β-amyrin synthase, lupeol synthase and cycloartenol synthase, respectively, by heterologous expression of the cDNAs in an OSC-disrupted yeast mutant. A phylogenetic tree based on deduced amino acid sequences categorized eudicot OSCs into four groups and suggested an extensive diversification of the group IV OSCs.
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Functional and structural analysis of genes encoding oxidosqualene cyclases of Lotus Japonicus
Plant Science, 2006Co-Authors: Satoru Sawai, Tamotsu Shindo, Takakazu Kaneko, Shin Ichi Ayabe, Shusei Sato, Satoshi Tabata, Toshio AokiAbstract:Abstract The structures of cyclic triterpenoids of higher plants, whose skeletons are constructed by oxidosqualene cyclases (OSCs), are remarkably diverse in contrast to the limited variation in sterol/triterpenoid structures of other organisms. We comprehensively analyzed the functions and structures of OSC genes of a model legume, Lotus Japonicus , and compared them with those of Arabidopsis thaliana in order to elucidate the diversification process of plant cyclic triterpenoids. Eight OSC genes ( OSC1 – OSC8 ) formed two sets of clusters in chromosomes 2 and 3 of L. Japonicus . OSC1 and OSC8 corresponded to the previously reported LjAMY1 and LjAMY2, respectively [I. Iturbe-Ormaetxe, K. Haralampidis, K. Papadopoulou, A.E. Osbourn, Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus Japonicus , Plant Mol. Biol. 51 (2003) 731–743]. OSC1 (the full-length version of LjAMY1), OSC3 and OSC5 were identified as β-amyrin synthase, lupeol synthase and cycloartenol synthase, respectively, by heterologous expression of the cDNAs in an OSC-disrupted yeast mutant. A phylogenetic tree based on deduced amino acid sequences categorized eudicot OSCs into four groups and suggested an extensive diversification of the group IV OSCs.
Da Luo - One of the best experts on this subject based on the ideXlab platform.
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Functional diversity of CYCLOIDEA-like TCP genes in the control of zygomorphic flower development in Lotus Japonicus.
Journal of integrative plant biology, 2013Co-Authors: Yonghai Luo, Jun Yang, Zhigang Cai, Xiangling Cao, Da LuoAbstract:CYCLOIDEA (CYC)-like TCP genes play key roles in dorsoventral differentiation of zygomorphic flowers in Papilionoideae legumes. In this study, we analyzed the kew mutants whose flowers lost lateral identity, and investigated the diverse functions of three LjCYC genes during zygomorphic flower development in the model legume Lotus Japonicus. We showed that kew1 and kew3 are allelic mutants of LjCYC3, a CYC-like TCP gene. Through transgenic experiments, it was shown that LjCYC1 possesses dorsal activity similar to LjCYC2, and that LjCYC3 alone is sufficient to confer lateral activity, and an epistatic effect between dorsal and lateral activities was identified. Sequence analysis revealed a striking alteration at the 3' end of the LjCYC3 open reading frame (ORF) in comparison with those of LjCYC1 and LjCYC2 ORFs. Furthermore, it was found that LjCYC proteins could interact with each other and possess different activities by means of a transcriptional activity assay. Our data demonstrate that the sequence variation and the subsequent alteration of protein property play important roles in the functional diversity of different LjCYC genes in controlling zygomorphic flower development in Lotus Japonicus.
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Research Article Functional Diversity of CYCLOIDEA-like TCP Genes in the Control of Zygomorphic Flower Development in Lotus Japonicus
2013Co-Authors: Yonghai Luo, Jun Yang, Zhigang Cai, Xiangling Cao, Da LuoAbstract:CYCLOIDEA (CYC)-like TCP genes play key roles in dorsoventral differentiation of zygomorphic flowers in Papilionoideae legumes. In this study, we analyzed the kew mutants whose flowers lost lateral identity, and investigated the diverse functions of three LjCYC genes during zygomorphic flower development in the model legume Lotus Japonicus. We showed that kew1 and kew3 are allelic mutants of LjCYC3 ,a CYC- like TCP gene. Through transgenic experiments, it was shown that LjCYC1 possesses dorsal activity similar to LjCYC2 ,a nd thatLjCYC3 alone is sufficient to confer lateral activity, and an epistatic effect between dorsal and lateral activities was identified. Sequence analysis revealed a striking alteration at the 3 � end of the LjCYC3 open reading frame (ORF) in comparison with those of LjCYC1 and LjCYC2 ORFs. Furthermore, it was found that LjCYC proteins could interact with each other and possess different activities by means of a transcriptional activity assay. Our data demonstrate that the sequence variation and the subsequent alteration of protein property play important roles in the functional diversity of different LjCYC genes in controlling zygomorphic flower development in Lotus Japonicus.
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Petal Development in Lotus Japonicus.
Journal of integrative plant biology, 2011Co-Authors: Lin Weng, Xian-zhong Feng, Da Luo, Zhaoxia Tian, Jun YangAbstract:Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms, dorsoventral (DV) and organ internal (IN) asymmetric mechanisms, respectively. However, little is known about the molecular mechanisms controlling petal development in legumes. To address this question, we investigated petal development along the floral DV axis in Lotus Japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants. Based on morphological markers, the entire course of petal development, from initiation to maturity, was grouped to define 3 phases or 13 stages. In terms of epidermal micromorphology from adaxial surface, mature petals were divided into several distinct domains, and characteristic epidermal cells of each petal differentiated at stage 9, while epidermal cells of all domains were observed until stage 12. TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12. Our results suggest that DV and IN mechanisms interplay at different stages of petal development, and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape, and further suggest that TCP genes determine petal identity along the DV axis by regulating MIXTA-like gene expression.
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Research Article Petal Development in Lotus Japonicus
2011Co-Authors: Lin Weng, Xian-zhong Feng, Da Luo, Zhaoxia Tian, Jun YangAbstract:Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms, dorsoventral (DV) and organ internal (IN) asymmetric mechanisms, respectively. However, little is known about the molecular mechanisms controlling petal development in legumes. To address this question, we investigated petal development along the floral DV axis in Lotus Japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants. Based on morphologicalmarkers,theentirecourseofpetaldevelopment,frominitiationtomaturity,wasgroupedto define 3 phases or 13 stages. In terms of epidermal micromorphology from adaxial surface, mature petals were divided into several distinct domains, and characteristic epidermal cells of each petal differentiated at stage 9, while epidermal cells of all domains were observed until stage 12. TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12. Our results suggest that DV and IN mechanisms interplay at different stages of petal development, and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape, and further suggest that TCP genes determine petal identity along the DV axis by regulating MIXTA-like gene expression.
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Mutations Affecting Lateral Petal Development in Lotus Japonicus
Legume Genomics and Genetics, 2010Co-Authors: Jiechen Wang, Zhaoxia Tian, Zhong Zhao, Da LuoAbstract:To investigate the genetic loci to regulate the development of petal shape, screening of the mutation affecting lateral petal development was conducted in a model plant of legume, Lotus Japonicus . Two independent mutants, kew2 and kew3 with the similar phenotype were obtained, whose shape of lateral petal is altered and resembles the one of ventral petal in the wild type. Genetic analysis showed that kew3 was caused by the same single recessive locus and allelic to the one of kew1 , a formal identified mutant in the locus KEW1 . KEW1 has been shown to be an ortholog of K in pea, but whose mutation at the molecular level is unsolved yet. Furthermore, genetic analyses of kew2 indicate that there are other genetic factors which could interact with kew and involve in the lateral petal development. Analysis of these mutations and cloning the corresponding loci will shield light on the underlying molecular mechanism in the control of floral asymmetry and facilitate our understanding on the evolution of the zygomorphic development.
Toshio Aoki - One of the best experts on this subject based on the ideXlab platform.
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Mutants of Lotus Japonicus deficient in flavonoid biosynthesis
Journal of Plant Research, 2021Co-Authors: Toshio Aoki, Shin Ichi Ayabe, Masayoshi Kawaguchi, Haruko Imaizumi-anraku, Shoichiro Akao, Tomoyoshi AkashiAbstract:Spatiotemporal features of anthocyanin accumulation in a model legume Lotus Japonicus (Regel) K.Larsen were elucidated to develop criteria for the genetic analysis of flavonoid biosynthesis. Artificial mutants and wild accessions, with lower anthocyanin accumulation in the stem than the standard wild type (B-129 ‘Gifu’), were obtained by ethyl methanesulfonate (EMS) mutagenesis and from a collection of wild-grown variants, respectively. The loci responsible for the green stem of the mutants were named as VI RIDI C AULIS ( VIC ). Genetic and chemical analysis identified two loci, namely, VIC1 and VIC2 , required for the production of both anthocyanins and proanthocyanidins (condensed tannins), and two loci, namely, VIC3 and VIC4 , required for the steps specific to anthocyanin biosynthesis. A mutation in VIC5 significantly reduced the anthocyanin accumulation. These mutants will serve as a useful system for examining the effects of anthocyanins and proanthocyanidins on the interactions with herbivorous pests, pathogenic microorganisms and nitrogen-fixing symbiotic bacteria, Mesorhizobium loti .
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Discriminative Phytoalexin Accumulation in Lotus Japonicus against Symbiotic and Non-Symbiotic Microorganisms and Related Chemical Signals
Bioscience biotechnology and biochemistry, 2013Co-Authors: Motomu Masai, Shin Ichi Ayabe, Toshio Aoki, Tomomi Nakagawa, Miho Arakawa, Koichi Iwaya, Hiroshi UchiyamaAbstract:The phytoalexin response of Lotus Japonicus seedlings to selected microbes and chemical signals was analyzed. The symbiotic rhizobium induced vestitol production weakly, while non-symbiotic rhizobia and potential pathogens led to increases in its accumulation. Whereas chitin-related molecules were ineffective, a flagellin-derived peptide not of symbiont origin induced phytoalexin production, indicating discriminative antibiotic production by the plant host.
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Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus Japonicus
Journal of plant research, 2010Co-Authors: Kazuko Yoshida, Shin Ichi Ayabe, Toshio Aoki, Rieko Iwasaka, Norimoto Shimada, Masaaki SakutaAbstract:In the genome of the model legume Lotus Japonicus, dihydroflavonol 4-reductase (DFR), which is the first committed enzyme of the anthocyanin and proanthocyanidin (PA) pathways, is encoded as a tandemly arrayed five-gene family. Expression analysis revealed that both organ specificity and stress responsiveness differ among the DFRs. To elucidate the regulatory mechanisms underlying the expression of DFRs, we investigated the transcriptional control of each member of the DFR multigene family. Ectopic expression of a combination of the transcription factors MYB, bHLH, and WDR showed that only the DFR2 promoter was activated, indicating that each member of the DFR gene family is regulated independently.
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Functional and structural analysis of genes encoding oxidosqualene cyclases of Lotus Japonicus
Plant Science, 2006Co-Authors: Satoru Sawai, Tamotsu Shindo, Takakazu Kaneko, Shin Ichi Ayabe, Shusei Sato, Satoshi Tabata, Toshio AokiAbstract:Abstract The structures of cyclic triterpenoids of higher plants, whose skeletons are constructed by oxidosqualene cyclases (OSCs), are remarkably diverse in contrast to the limited variation in sterol/triterpenoid structures of other organisms. We comprehensively analyzed the functions and structures of OSC genes of a model legume, Lotus Japonicus , and compared them with those of Arabidopsis thaliana in order to elucidate the diversification process of plant cyclic triterpenoids. Eight OSC genes ( OSC1 – OSC8 ) formed two sets of clusters in chromosomes 2 and 3 of L. Japonicus . OSC1 and OSC8 corresponded to the previously reported LjAMY1 and LjAMY2, respectively [I. Iturbe-Ormaetxe, K. Haralampidis, K. Papadopoulou, A.E. Osbourn, Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus Japonicus , Plant Mol. Biol. 51 (2003) 731–743]. OSC1 (the full-length version of LjAMY1), OSC3 and OSC5 were identified as β-amyrin synthase, lupeol synthase and cycloartenol synthase, respectively, by heterologous expression of the cDNAs in an OSC-disrupted yeast mutant. A phylogenetic tree based on deduced amino acid sequences categorized eudicot OSCs into four groups and suggested an extensive diversification of the group IV OSCs.
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Functional and structural analysis of genes encoding oxidosqualene cyclases of Lotus Japonicus
Plant Science, 2006Co-Authors: Satoru Sawai, Tamotsu Shindo, Takakazu Kaneko, Shin Ichi Ayabe, Shusei Sato, Satoshi Tabata, Toshio AokiAbstract:Abstract The structures of cyclic triterpenoids of higher plants, whose skeletons are constructed by oxidosqualene cyclases (OSCs), are remarkably diverse in contrast to the limited variation in sterol/triterpenoid structures of other organisms. We comprehensively analyzed the functions and structures of OSC genes of a model legume, Lotus Japonicus , and compared them with those of Arabidopsis thaliana in order to elucidate the diversification process of plant cyclic triterpenoids. Eight OSC genes ( OSC1 – OSC8 ) formed two sets of clusters in chromosomes 2 and 3 of L. Japonicus . OSC1 and OSC8 corresponded to the previously reported LjAMY1 and LjAMY2, respectively [I. Iturbe-Ormaetxe, K. Haralampidis, K. Papadopoulou, A.E. Osbourn, Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus Japonicus , Plant Mol. Biol. 51 (2003) 731–743]. OSC1 (the full-length version of LjAMY1), OSC3 and OSC5 were identified as β-amyrin synthase, lupeol synthase and cycloartenol synthase, respectively, by heterologous expression of the cDNAs in an OSC-disrupted yeast mutant. A phylogenetic tree based on deduced amino acid sequences categorized eudicot OSCs into four groups and suggested an extensive diversification of the group IV OSCs.
Shusei Sato - One of the best experts on this subject based on the ideXlab platform.
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SEN1 gene from Lotus Japonicus MG20 improves nitrogen fixation and plant growth
Soil Science and Plant Nutrition, 2020Co-Authors: Yuki Nishida, Shusei Sato, Reona Hiraoka, Satomi Kawano, Norio Suganuma, Satoshi Watanabe, Toyoaki Anai, Susumu Arima, Akiyoshi Tominaga, Akihiro SuzukiAbstract:In Lotus Japonicus, quantitative trait loci (QTLs) for nitrogen fixation activity (acetylene reduction activity, ARA) and seed weight are located in the same region on chromosome 4 as the gene resp...
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Lotus Japonicus Genetic, Mutant, and Germplasm Resources.
Current protocols in plant biology, 2018Co-Authors: Masatsugu Hashiguchi, Stig Uggerhøj Andersen, Niels Sandal, Hidenori Tanaka, Melody Muguerza, Ryo Akashi, Shusei SatoAbstract:A quarter of a century has passed since Lotus Japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. Japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. Japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. Japonicus more easily accessible as a model system, especially for research groups new to L. Japonicus research. © 2018 by John Wiley & Sons, Inc.
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Legume and Lotus Japonicus Databases
Compendium of Plant Genomes, 2014Co-Authors: Hideki Hirakawa, Shusei Sato, Terry Mun, Stig Uggerhøj AndersenAbstract:Since the genome sequence of Lotus Japonicus, a model plant of family Fabaceae, was determined in 2008 (Sato et al. 2008), the genomes of other members of the Fabaceae family, soybean (Glycine max) (Schmutz et al. 2010) and Medicago truncatula (Young et al. 2011), have been sequenced. In this section, we introduce representative, publicly accessible online resources related to plant materials, integrated databases containing legume genome information, and databases for genome sequence and derived marker information of legume species including L. Japonicus.
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Lotus Japonicus ARPC1 Is Required for Rhizobial Infection
Plant physiology, 2012Co-Authors: Shakhawat Hossain, Shusei Sato, Satoshi Tabata, Jens Stougaard, Lene H. Madsen, Anna Jurkiewicz, Euan K. James, Jinqiu Liao, Loretta Ross, Krzysztof SzczyglowskiAbstract:Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus Japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. Japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. Japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.
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genome structure of the legume Lotus Japonicus
DNA Research, 2008Co-Authors: Shusei Sato, Takakazu Kaneko, Yasukazu Nakamura, Erika Asamizu, Tomohiko Kato, Mitsuteru Nakao, Shigemi Sasamoto, Akiko Watanabe, Akiko Ono, Kumiko KawashimaAbstract:The legume Lotus Japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. Japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. Japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.
