The Experts below are selected from a list of 1758 Experts worldwide ranked by ideXlab platform
Kenta Saito - One of the best experts on this subject based on the ideXlab platform.
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recent progress in Luminescent Proteins development
Current Opinion in Chemical Biology, 2015Co-Authors: Kenta Saito, Takeharu NagaiAbstract:Bioimaging requires not only high sensitivity but also minimal invasiveness. Bioimaging using Luminescent Proteins is potentially free from problems such as photo-induced damage or an undesirable physical reaction to the sample, which are often caused by illumination with an external light required in fluorescence imaging. The recent development of several Luminescent Proteins and substrates have greatly improved the brightness of luminescence imaging, facilitating its application by many researchers. In this short review, we summarize recent advances in development of Luminescent Proteins, substrates, and indicators.
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Luminescent Proteins for high speed single cell and whole body imaging
Nature Communications, 2012Co-Authors: Kenta Saito, Yufen Chang, Kazuki Horikawa, Noriyuki Hatsugai, Yuriko Higuchi, Mitsuru Hashida, Yu Yoshida, Tomoki MatsudaAbstract:The use of fluorescent Proteins has revolutionized our understanding of biological processes. However, the requirement for external illumination precludes their universal application to the study of biological processes in all tissues. Although light can be created by chemiluminescence, light emission from existing chemiLuminescent probes is too weak to use this imaging modality in situations when fluorescence cannot be used. Here we report the development of the brightest Luminescent protein to date, Nano-lantern, which is a chimera of enhanced Renilla luciferase and Venus, a fluorescent protein with high bioluminescence resonance energy transfer efficiency. Nano-lantern allows real-time imaging of intracellular structures in living cells with spatial resolution equivalent to fluorescence and sensitive tumour detection in freely moving unshaved mice. We also create functional indicators based on Nano-lantern that can image Ca(2+), cyclic adenosine monophosphate and adenosine 5'-triphosphate dynamics in environments where the use of fluorescent indicators is not feasible. These Luminescent Proteins allow visualization of biological phenomena at previously unseen single-cell, organ and whole-body level in animals and plants.
Tomoki Matsuda - One of the best experts on this subject based on the ideXlab platform.
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Luminescent Proteins for high speed single cell and whole body imaging
Nature Communications, 2012Co-Authors: Kenta Saito, Yufen Chang, Kazuki Horikawa, Noriyuki Hatsugai, Yuriko Higuchi, Mitsuru Hashida, Yu Yoshida, Tomoki MatsudaAbstract:The use of fluorescent Proteins has revolutionized our understanding of biological processes. However, the requirement for external illumination precludes their universal application to the study of biological processes in all tissues. Although light can be created by chemiluminescence, light emission from existing chemiLuminescent probes is too weak to use this imaging modality in situations when fluorescence cannot be used. Here we report the development of the brightest Luminescent protein to date, Nano-lantern, which is a chimera of enhanced Renilla luciferase and Venus, a fluorescent protein with high bioluminescence resonance energy transfer efficiency. Nano-lantern allows real-time imaging of intracellular structures in living cells with spatial resolution equivalent to fluorescence and sensitive tumour detection in freely moving unshaved mice. We also create functional indicators based on Nano-lantern that can image Ca(2+), cyclic adenosine monophosphate and adenosine 5'-triphosphate dynamics in environments where the use of fluorescent indicators is not feasible. These Luminescent Proteins allow visualization of biological phenomena at previously unseen single-cell, organ and whole-body level in animals and plants.
Se Won Bae - One of the best experts on this subject based on the ideXlab platform.
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camp biosensors based on genetically encoded fluorescent Luminescent Proteins
Biosensors, 2021Co-Authors: Namdoo Kim, Seunghan Shin, Se Won BaeAbstract:Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some cancers and prefrontal cortex disorders. For example, modulation of cAMP-mediated intracellular signaling pathways by anti-tumor drugs could reduce tumor growth. However, most early stage tools used for measuring the cAMP level in living organisms require cell disruption, which is not appropriate for live cell imaging or animal imaging. Thus, in the last decades, tools were developed for real-time monitoring of cAMP distribution or signaling dynamics in a non-invasive manner. Genetically-encoded sensors based on fluorescent Proteins and luciferases could be powerful tools to overcome these drawbacks. In this review, we discuss the recent genetically-encoded cAMP sensors advances, based on single fluorescent protein (FP), Foster resonance energy transfer (FRET), single luciferase, and bioluminescence resonance energy transfer (BRET) for real-time non-invasive imaging.
Noriyuki Hatsugai - One of the best experts on this subject based on the ideXlab platform.
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Luminescent Proteins for high speed single cell and whole body imaging
Nature Communications, 2012Co-Authors: Kenta Saito, Yufen Chang, Kazuki Horikawa, Noriyuki Hatsugai, Yuriko Higuchi, Mitsuru Hashida, Yu Yoshida, Tomoki MatsudaAbstract:The use of fluorescent Proteins has revolutionized our understanding of biological processes. However, the requirement for external illumination precludes their universal application to the study of biological processes in all tissues. Although light can be created by chemiluminescence, light emission from existing chemiLuminescent probes is too weak to use this imaging modality in situations when fluorescence cannot be used. Here we report the development of the brightest Luminescent protein to date, Nano-lantern, which is a chimera of enhanced Renilla luciferase and Venus, a fluorescent protein with high bioluminescence resonance energy transfer efficiency. Nano-lantern allows real-time imaging of intracellular structures in living cells with spatial resolution equivalent to fluorescence and sensitive tumour detection in freely moving unshaved mice. We also create functional indicators based on Nano-lantern that can image Ca(2+), cyclic adenosine monophosphate and adenosine 5'-triphosphate dynamics in environments where the use of fluorescent indicators is not feasible. These Luminescent Proteins allow visualization of biological phenomena at previously unseen single-cell, organ and whole-body level in animals and plants.
Yuriko Higuchi - One of the best experts on this subject based on the ideXlab platform.
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Luminescent Proteins for high speed single cell and whole body imaging
Nature Communications, 2012Co-Authors: Kenta Saito, Yufen Chang, Kazuki Horikawa, Noriyuki Hatsugai, Yuriko Higuchi, Mitsuru Hashida, Yu Yoshida, Tomoki MatsudaAbstract:The use of fluorescent Proteins has revolutionized our understanding of biological processes. However, the requirement for external illumination precludes their universal application to the study of biological processes in all tissues. Although light can be created by chemiluminescence, light emission from existing chemiLuminescent probes is too weak to use this imaging modality in situations when fluorescence cannot be used. Here we report the development of the brightest Luminescent protein to date, Nano-lantern, which is a chimera of enhanced Renilla luciferase and Venus, a fluorescent protein with high bioluminescence resonance energy transfer efficiency. Nano-lantern allows real-time imaging of intracellular structures in living cells with spatial resolution equivalent to fluorescence and sensitive tumour detection in freely moving unshaved mice. We also create functional indicators based on Nano-lantern that can image Ca(2+), cyclic adenosine monophosphate and adenosine 5'-triphosphate dynamics in environments where the use of fluorescent indicators is not feasible. These Luminescent Proteins allow visualization of biological phenomena at previously unseen single-cell, organ and whole-body level in animals and plants.
