The Experts below are selected from a list of 5430 Experts worldwide ranked by ideXlab platform
Bernard Fried - One of the best experts on this subject based on the ideXlab platform.
-
Zygocotyle Lunata: proteomic analysis of the adult stage.
Experimental Parasitology, 2011Co-Authors: Javier Sotillo, Bernard Fried, M. Luz Valero, Manuel M. Sánchez Del Pino, J. Guillermo Esteban, Antonio Marcilla, Rafael ToledoAbstract:The somatic extract of Zygocotyle Lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. Lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. Lunata. This is the first study that attempts to identify the proteome of Z. Lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes.
-
Histopathological effects of the intramolluscan stages of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae on Helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata , Echinostoma trivolvis , and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata . Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae . The presence of keratin is probably related to its role in cercarial encystment.
-
histopathological effects of the intramolluscan stages of zygocotyle Lunata echinostoma trivolvis and ribeiroia ondatrae on helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata. Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae. The presence of keratin is probably related to its role in cercarial encystment.
-
The association of Zygocotyle Lunata and Echinostoma trivolvis with Chaetogaster limnaei, an ectosymbiont of Helisoma trivolvis.
Journal of Parasitology, 2008Co-Authors: Bernard Fried, Robert C. Peoples, Tyler M. Saxton, Jane E. HuffmanAbstract:Helisoma trivolvis snails collected from a lake in Warren County, New Jersey, in June 2007 possessed the ectosymbiont Chaetogaster limnaei (Annelida). Some of these snails were also infected with larval stages of Zygocotyle Lunata and Echinostoma trivolvis. Chaetogaster limnaei associated with the infected snails fed on the cercariae of both digeneans. Zygocotylae Lunata cercariae were observed in the stomach of C. limnaei and whole cercariae were loosely attached to the ventral surface of the chaetogasters. Cercariae in the stomachs were digested within 48 hr and probably served as a source of nutrient for the annelids. Whole cercariae and 1 nonviable metacercaria of E. trivolvis were seen in the stomachs of the chaetogasters. The protective action of the chaetogasters on the transmission of the cercariae of E. trivolvis and Z. Lunata to second intermediate hosts in the wild awaits further study.
-
Infectivity, growth, survival, and pathogenicity of Zygocotyle Lunata (Trematoda) in experimental rodent hosts.
Journal of Parasitology, 1991Co-Authors: Jane E. Huffman, Christine Sabol, Bernard FriedAbstract:Laboratory mice, rats, and golden hamsters were fed metacercarial cysts of Zygocotyle Lunata to examine infectivity, growth, survival, and pathogenicity of this trematode. All 3 rodent types became infected with Z. Lunata. Eggs of Z. Lunata were seen in the feces of the hamsters by day 21, in mice by day 26, and in rats by day 44. Eggs teased from worms and embryonated in tap water hatched from day 21 to day 26 for rats and mice and from day 40 to day 45 for hamsters. The body areas of sexually mature worms were similar in all 3 types of rodent species. It was possible to reinfect all 3 species with Z. Lunata metacercariae. No sign of clinical amphistomiasis was evident in the experimental animals. The histopathological responses were progressive, and severity was related to the age of the infection and the number of worms in the infection.
Jane E. Huffman - One of the best experts on this subject based on the ideXlab platform.
-
histopathological effects of the intramolluscan stages of zygocotyle Lunata echinostoma trivolvis and ribeiroia ondatrae on helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata. Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae. The presence of keratin is probably related to its role in cercarial encystment.
-
Histopathological effects of the intramolluscan stages of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae on Helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata , Echinostoma trivolvis , and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata . Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae . The presence of keratin is probably related to its role in cercarial encystment.
-
The association of Zygocotyle Lunata and Echinostoma trivolvis with Chaetogaster limnaei, an ectosymbiont of Helisoma trivolvis.
Journal of Parasitology, 2008Co-Authors: Bernard Fried, Robert C. Peoples, Tyler M. Saxton, Jane E. HuffmanAbstract:Helisoma trivolvis snails collected from a lake in Warren County, New Jersey, in June 2007 possessed the ectosymbiont Chaetogaster limnaei (Annelida). Some of these snails were also infected with larval stages of Zygocotyle Lunata and Echinostoma trivolvis. Chaetogaster limnaei associated with the infected snails fed on the cercariae of both digeneans. Zygocotylae Lunata cercariae were observed in the stomach of C. limnaei and whole cercariae were loosely attached to the ventral surface of the chaetogasters. Cercariae in the stomachs were digested within 48 hr and probably served as a source of nutrient for the annelids. Whole cercariae and 1 nonviable metacercaria of E. trivolvis were seen in the stomachs of the chaetogasters. The protective action of the chaetogasters on the transmission of the cercariae of E. trivolvis and Z. Lunata to second intermediate hosts in the wild awaits further study.
-
Infectivity, growth, survival, and pathogenicity of Zygocotyle Lunata (Trematoda) in experimental rodent hosts.
Journal of Parasitology, 1991Co-Authors: Jane E. Huffman, Christine Sabol, Bernard FriedAbstract:Laboratory mice, rats, and golden hamsters were fed metacercarial cysts of Zygocotyle Lunata to examine infectivity, growth, survival, and pathogenicity of this trematode. All 3 rodent types became infected with Z. Lunata. Eggs of Z. Lunata were seen in the feces of the hamsters by day 21, in mice by day 26, and in rats by day 44. Eggs teased from worms and embryonated in tap water hatched from day 21 to day 26 for rats and mice and from day 40 to day 45 for hamsters. The body areas of sexually mature worms were similar in all 3 types of rodent species. It was possible to reinfect all 3 species with Z. Lunata metacercariae. No sign of clinical amphistomiasis was evident in the experimental animals. The histopathological responses were progressive, and severity was related to the age of the infection and the number of worms in the infection.
Jie Chen - One of the best experts on this subject based on the ideXlab platform.
-
thc6 protein isolated from trichoderma harzianum can induce maize defense response against curvularia Lunata
Journal of Basic Microbiology, 2015Co-Authors: Kehe Fu, Yaqian Li, Yingying Li, Chuanjin Yu, Jie ChenAbstract:: Mutant T66 was isolated from 450 mutants (constructed with Agrobacterium tumefaciens-mediated transformation method) of Trichoderma harzianum. Maize seeds coated with T66 were more susceptible to Curvularia Lunata when compared with those coated with wild-type (WT) strain. The disease index of maize treated with T66 and WT were 62.5 and 42.1%, respectively. Further research showed T-DNA has inserted into the ORF of one gene, which resulted in the functional difference between WT and T66. The gene was cloned and named Thc6, which encodes a novel 327 amino acid protein. To investigate its function, we obtained knockout, complementation, and overexpression mutants of Thc6. Challenge inoculation studies suggested that the Thc6 overexpression mutant can reduce the disease index of maize inbred line Huangzao 4 against the leaf spot pathogen (C. Lunata). Meanwhile, The Thc6 mutants were found to affect the resistance of maize inbred line Huangzao 4 against C. Lunata by enhancing the activation of jasmonate-responsive genes expression. Liquid chromatography-mass spectrometry (LC-MS) data further confirmed that the concentration of jasmonate in the induced maize exhibits a parallel change tendency with the expression level of defense-related genes. Hence, the Thc6 gene could be participated in the induced resistance of maize inbred line Huangzao 4 against C. Lunata infection through a jasmonic acid-dependent pathway.
-
genome sequence and virulence variation related transcriptome profiles of curvularia Lunata an important maize pathogenic fungus
BMC Genomics, 2014Co-Authors: Yaqian Li, Kehe Fu, Yingying Li, Jie ChenAbstract:Curvularia Lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. We report the genome sequences of a highly virulent C. Lunata strain. Phylogenomic analysis indicates that C. Lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. Lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. Lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. Lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. Lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps. The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. Lunata.
-
construction and functional analysis of trichoderma harzianum mutants that modulate maize resistance to the pathogen curvularia Lunata
Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes, 2014Co-Authors: Kehe Fu, Yaqian Li, Chuanjin Yu, Jie ChenAbstract:Agrobacterium tumefaciens-mediated transformation (ATMT) was used to generate an insertional mutant library of the mycelial fungus Trichoderma harzianum. From a total of 450 mutants, six mutants that showed significant influence on maize resistance to C. Lunata were analyzed in detail. Maize coated with these mutants was more susceptible to C. Lunata compared with those coated with a wild-type (WT) strain. Similar to other fungal ATMT libraries, all six mutants were single copy integrations, which occurred preferentially in noncoding regions (except two mutants) and were frequently accompanied by the loss of border sequences. Two mutants (T66 and T312) that were linked to resistance were characterized further. Maize seeds coated with T66 and T312 were more susceptible to C. Lunata than those treated with WT. Moreover, the mutants affected the resistance of maize to C. Lunata by enhancing jasmonate-responsive gene expression. T66 and T312 induced maize resistance to C. Lunata infection through a jasmonic a...
-
understanding resistant germplasm induced virulence variation through analysis of proteomics and suppression subtractive hybridization in a maize pathogen curvularia Lunata
Proteomics, 2012Co-Authors: Yingying Li, Kehe Fu, Qiong Wu, Jie ChenAbstract:: Curvularia Lunata is an important pathogen causing Curvularia leaf spot in maize. Significant pathogenic variation has been found in C. Lunata. To better understand the mechanism of this phenomenon, we consecutively put the selective pressures of resistant maize population on C. Lunata strain WS18 (low virulence) artificially. As a result, the virulence of this strain was significantly enhanced. Using 2DE, 12 up-regulated and four down-regulated proteins were identified in virulence-increased strain compared to WS18. Our analysis revealed that melanin synthesis-related proteins (Brn1, Brn2, and scytalone dehydratase) and stress tolerance-related proteins (HSP 70) directly involved in the potential virulence growth as crucial markers or factors in C. Lunata. To validate 2DE results and screen differential genes at mRNA level, we constructed a subtracted cDNA library (tester: virulence-increased strain; driver: WS18). A total of 188 unigenes were obtained this way, of which 14 were indicators for the evolution of pathogen virulence. Brn1 and hsp genes exhibited similar expression patterns corresponding to proteins detected by 2DE. Overall, our results indicated that differential proteins or genes, being involved with melanin synthesis or tolerance response to stress, could be considered as hallmarks of virulence increase in C. Lunata.
-
expression of green fluorescent protein in curvularia Lunata causing maize leaf spot
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2010Co-Authors: Guangji Li, Jie ChenAbstract:Curvularia Lunata is a widespread fungal pathogen infecting maize leaves. In this study, we developed a genetic transformation system for C. Lunata by using the plasmid (pCPXHY1Egfp) with both enhanced green fluorescent protein gene egfp and hygromycin resistance gene hph. GFP rendered hyphae of the transformants fluorescent in culture. Most transformants that showed constitutively expressing EGFP were comparable to the wild-type strain in morphological features. PCR and Southern blot analyses of the hph gene further confirmed that the plasmid was integrated into the genome of C. Lunata. This is the first report that a ‘reporter’ gene, egfp, could be stably expressed in C. Lunata. Using the transformants with EGFP marker will assist us to visualize the features of infection at different stages.
Jennifer Klockars - One of the best experts on this subject based on the ideXlab platform.
-
Histopathological effects of the intramolluscan stages of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae on Helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata , Echinostoma trivolvis , and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata . Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae . The presence of keratin is probably related to its role in cercarial encystment.
-
histopathological effects of the intramolluscan stages of zygocotyle Lunata echinostoma trivolvis and ribeiroia ondatrae on helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata. Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae. The presence of keratin is probably related to its role in cercarial encystment.
Shamus P. Keeler - One of the best experts on this subject based on the ideXlab platform.
-
Histopathological effects of the intramolluscan stages of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae on Helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata , Echinostoma trivolvis , and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata . Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae . The presence of keratin is probably related to its role in cercarial encystment.
-
histopathological effects of the intramolluscan stages of zygocotyle Lunata echinostoma trivolvis and ribeiroia ondatrae on helisoma trivolvis and observations on keratin in the trematode larvae
Parasitology Research, 2009Co-Authors: Jane E. Huffman, Jennifer Klockars, Shamus P. Keeler, Bernard FriedAbstract:The histopathological effects of Zygocotyle Lunata, Echinostoma trivolvis, and Ribeiroia ondatrae in naturally infected Helisoma trivolvis were studied in hematoxylin and eosin sections of infected digestive glands fixed in 10% neutral buffered formalin. The larval stages of all three trematodes damaged the snail digestive gland. Most notable histopathology included disrupted digestive gland tubules, lysed cells, compressed tubules, and edematous spaces between tubules. Considerable damage was done by rediae ingesting digestive cells. There was a detectable hemocytic response by H. trivolvis in response to the rediae and cercariae of Z. Lunata. Histochemical studies on sectioned material stained with the Ayoub-Shklar method for keratin detected the presence of this protein in the rediae and cercariae of Z. Lunata and R. ondatrae. The presence of keratin is probably related to its role in cercarial encystment.
