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Pim Lindhout - One of the best experts on this subject based on the ideXlab platform.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Caicheng Huang, Fien Meijerdekens, Guusje Bonnema, Ron Van Der Hulst, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F-2 population from a cross of Lycopersicon esculentum cv. Moneymaker x Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F-3 progenies. A set of polymerase chain reaction-based cleaved amplified pollymorphic sequence and sequence characterized amplified region markers was generated for efficient monitoring of the target QTL genomic regions in marker assisted selection. The possible relationship between genes underlying major and partial resistance for tomato powdery mildew is discussed.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Yuling Bai, Caicheng Huang, Fien Meijerdekens, Ron Van Der Hulst, Guusje Bonnema, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F2 population from a cross of Lycopersicon esculentum cv. Moneymaker × Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F3 progenies. A set of polymerase chain reaction-based cleaved amplified polymorphic sequence and sequence characterized amplified region ma...

  • the resistance to powdery mildew oidium lycopersicum in Lycopersicon species is mainly associated with hypersensitive response
    European Journal of Plant Pathology, 1998
    Co-Authors: Caicheng Huang, Ton Groot, Fien Meijerdekens, Rients E. Niks, Pim Lindhout
    Abstract:

    The cultivated tomato ( Lycopersicon esculentum) is susceptible to powdery mildew ( Oidium lycopersicum). Six accessions of three related Lycopersicon species show high levels of resistance (Lindhout et al., 1994b). The present research aimed at describing the development of O. lycopersicum on susceptible cv Moneymaker and characterizing the defence response to O. lycopersicum in Lycopersicon accessions by histological analysis. Spore germination and (primary) haustorium formation in resistant accessions were as frequent as in the susceptible L. esculentum cv Moneymaker. A high frequency of necrosis of epidermal cells in which a haustorium was formed appeared to be the major defence response, indicating that resistance to O. lycopersicum in the Lycopersicon genus was predominantly based on the hypersensitive reaction. However, the resistance in L. parviflorum was less associated with hypersensitivity than in other resistant accessions, suggesting the existence of a different but still unknown resistance mechanism. In addition, evidence is provided that the level of resistance could depend on the genetic background and the plant age.

Caicheng Huang - One of the best experts on this subject based on the ideXlab platform.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Caicheng Huang, Fien Meijerdekens, Guusje Bonnema, Ron Van Der Hulst, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F-2 population from a cross of Lycopersicon esculentum cv. Moneymaker x Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F-3 progenies. A set of polymerase chain reaction-based cleaved amplified pollymorphic sequence and sequence characterized amplified region markers was generated for efficient monitoring of the target QTL genomic regions in marker assisted selection. The possible relationship between genes underlying major and partial resistance for tomato powdery mildew is discussed.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Yuling Bai, Caicheng Huang, Fien Meijerdekens, Ron Van Der Hulst, Guusje Bonnema, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F2 population from a cross of Lycopersicon esculentum cv. Moneymaker × Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F3 progenies. A set of polymerase chain reaction-based cleaved amplified polymorphic sequence and sequence characterized amplified region ma...

  • the resistance to powdery mildew oidium lycopersicum in Lycopersicon species is mainly associated with hypersensitive response
    European Journal of Plant Pathology, 1998
    Co-Authors: Caicheng Huang, Ton Groot, Fien Meijerdekens, Rients E. Niks, Pim Lindhout
    Abstract:

    The cultivated tomato ( Lycopersicon esculentum) is susceptible to powdery mildew ( Oidium lycopersicum). Six accessions of three related Lycopersicon species show high levels of resistance (Lindhout et al., 1994b). The present research aimed at describing the development of O. lycopersicum on susceptible cv Moneymaker and characterizing the defence response to O. lycopersicum in Lycopersicon accessions by histological analysis. Spore germination and (primary) haustorium formation in resistant accessions were as frequent as in the susceptible L. esculentum cv Moneymaker. A high frequency of necrosis of epidermal cells in which a haustorium was formed appeared to be the major defence response, indicating that resistance to O. lycopersicum in the Lycopersicon genus was predominantly based on the hypersensitive reaction. However, the resistance in L. parviflorum was less associated with hypersensitivity than in other resistant accessions, suggesting the existence of a different but still unknown resistance mechanism. In addition, evidence is provided that the level of resistance could depend on the genetic background and the plant age.

Fien Meijerdekens - One of the best experts on this subject based on the ideXlab platform.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Caicheng Huang, Fien Meijerdekens, Guusje Bonnema, Ron Van Der Hulst, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F-2 population from a cross of Lycopersicon esculentum cv. Moneymaker x Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F-3 progenies. A set of polymerase chain reaction-based cleaved amplified pollymorphic sequence and sequence characterized amplified region markers was generated for efficient monitoring of the target QTL genomic regions in marker assisted selection. The possible relationship between genes underlying major and partial resistance for tomato powdery mildew is discussed.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Yuling Bai, Caicheng Huang, Fien Meijerdekens, Ron Van Der Hulst, Guusje Bonnema, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F2 population from a cross of Lycopersicon esculentum cv. Moneymaker × Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F3 progenies. A set of polymerase chain reaction-based cleaved amplified polymorphic sequence and sequence characterized amplified region ma...

  • the resistance to powdery mildew oidium lycopersicum in Lycopersicon species is mainly associated with hypersensitive response
    European Journal of Plant Pathology, 1998
    Co-Authors: Caicheng Huang, Ton Groot, Fien Meijerdekens, Rients E. Niks, Pim Lindhout
    Abstract:

    The cultivated tomato ( Lycopersicon esculentum) is susceptible to powdery mildew ( Oidium lycopersicum). Six accessions of three related Lycopersicon species show high levels of resistance (Lindhout et al., 1994b). The present research aimed at describing the development of O. lycopersicum on susceptible cv Moneymaker and characterizing the defence response to O. lycopersicum in Lycopersicon accessions by histological analysis. Spore germination and (primary) haustorium formation in resistant accessions were as frequent as in the susceptible L. esculentum cv Moneymaker. A high frequency of necrosis of epidermal cells in which a haustorium was formed appeared to be the major defence response, indicating that resistance to O. lycopersicum in the Lycopersicon genus was predominantly based on the hypersensitive reaction. However, the resistance in L. parviflorum was less associated with hypersensitivity than in other resistant accessions, suggesting the existence of a different but still unknown resistance mechanism. In addition, evidence is provided that the level of resistance could depend on the genetic background and the plant age.

Guusje Bonnema - One of the best experts on this subject based on the ideXlab platform.

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Yuling Bai, Caicheng Huang, Fien Meijerdekens, Ron Van Der Hulst, Guusje Bonnema, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F2 population from a cross of Lycopersicon esculentum cv. Moneymaker × Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F3 progenies. A set of polymerase chain reaction-based cleaved amplified polymorphic sequence and sequence characterized amplified region ma...

  • qtls for tomato powdery mildew resistance oidium lycopersici in Lycopersicon parviflorum g1 1601 co localize with two qualitative powdery mildew resistance genes
    Molecular Plant-microbe Interactions, 2003
    Co-Authors: Caicheng Huang, Fien Meijerdekens, Guusje Bonnema, Ron Van Der Hulst, Pim Lindhout
    Abstract:

    Tomato (Lycopersicon esculentum) is susceptible to the powdery mildew Oidium lycopersici, but several wild relatives such as Lycopersicon parviflorum G1.1601 are completely resistant. An F-2 population from a cross of Lycopersicon esculentum cv. Moneymaker x Lycopersicon parviflorum G1.1601 was used to map the O. lycopersici resistance by using amplified fragment length polymorphism markers. The resistance was controlled by three quantitative trait loci (QTLs). Ol-qtl1 is on chromosome 6 in the same region as the Ol-1 locus, which is involved in a hypersensitive resistance response to O. lycopersici. Ol-qtl2 and Ol-qtl3 are located on chromosome 12, separated by 25 cM, in the vicinity of the Lv locus conferring resistance to another powdery mildew species, Leveillula taurica. The three QTLs, jointly explaining 68% of the phenotypic variation, were confirmed by testing F-3 progenies. A set of polymerase chain reaction-based cleaved amplified pollymorphic sequence and sequence characterized amplified region markers was generated for efficient monitoring of the target QTL genomic regions in marker assisted selection. The possible relationship between genes underlying major and partial resistance for tomato powdery mildew is discussed.

Yuri Y. Gleba - One of the best experts on this subject based on the ideXlab platform.

  • Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun.
    Molecular Genetics and Genomics, 1993
    Co-Authors: Yakov I. Ratushnyak, Nickolai N. Cherep, Anna V. Zavgorodnyaya, Sergei A. Latypov, Irina V. Borozenko, Raiysa I. Rachkovskaya, Yuri Y. Gleba
    Abstract:

    Thirteen nuclear asymmetric hybrids were regenerated under selective conditions following fusion of chlorophyll-deficient protoplasts from cultivated tomato (Lycopersicon esculentum Mill.) and γ-(-irradiated protoplasts from the wild species Lycopersicon peruvianum var. dentatum Dun. All hybrid plants were classified as being asymmetric based on morphological traits, chromosome numbers and isozyme patterns. The majority of the hybrids inherited Lycopersicon peruvianum var. dentatum chloroplasts. Mitochondrial DNA analysis revealed mixed mitochondria populations deriving from both parents in some of the hybrids and rearranged mitochondrial DNA in others. The asymmetric hybrids express some morphological traits that are not found in either of the parental species. Fertile F1 plants were obtained after self-pollination of the asymmetric hybrids in four cases. The results obtained confirm the potential of asymmetric hybridization as a new source of genetic variation, and as a method for transferring of a part of genetic material from donor to recipient, and demonstrate that it is possible to produce fertile somatic hybrids by this technique.