The Experts below are selected from a list of 2058 Experts worldwide ranked by ideXlab platform
Satyendra Gautam - One of the best experts on this subject based on the ideXlab platform.
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No induced mutagenesis in human Lymphoblast Cell Line and bacterial systems upon their prolonged sub-culturing in irradiated food blended media.
Journal of the science of food and agriculture, 2017Co-Authors: Sudhanshu Saxena, Jyoti Tripathi, Sanjeev Kumar, Satyendra GautamAbstract:Profound apprehension towards safety of irradiated food has remained a major cause behind tardy acceptance of this technology although it has immense socio-economic potential. Generation of in-depth scientific evidence will help to refute these apprehensions. With this prospective, the present study was undertaken where safety of various irradiated (Dmin up to 25 kGy) foods was evaluated through long-term exposure studies in models including human Lymphoblast TK6 Cell Line (100 generations) and Escherichia coli MG1655 Cells (exclusive sub-culturing in irradiated food medium for 3000 generations). Additionally, the Ames test, micronucleus test, comet assay, DNA sequencing and restriction profiling of phagemid DNA from E. coli Cells sub-cultured in irradiated food medium were also performed.; Results: No induced mutagenesis was observed in these Cells during long-term sub-culturing in various irradiated food medium. Also no change was observed in profiles of comet, micronucleus, restriction digestion, random amplification of polymorphic DNA as well as DNA sequences. The latter also ruled out the possibility of any silent mutation.; Conclusion: Findings of the current study thus provided credible molecular evidence supporting the safety of irradiated foods. This would be helpful in confidence building among consumers, entrepreneurs, and strengthening the overall food irradiation program to achieve 'food safety' and 'security'. © 2017 Society of Chemical Industry.; © 2017 Society of Chemical Industry.
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Lack of induced mutagenesis in E. coli or human Lymphoblast Cell Line upon long-term sub-culturing in medium from irradiated meat.
International journal of radiation biology, 2017Co-Authors: Sachin N. Hajare, Jyoti Tripathi, Satyendra GautamAbstract:Purpose: Current study was aimed to enhance the confidence of consumers as well as entrepreneurs towards food irradiation program.Materials and methods: In this work, safety of high dose (25 kGy) i...
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Natural Predominance of Abscisic Acid in Pongammia pinnata (“Karanj”) Honey Contributed to its Strong Antimutagenicity
Journal of agricultural and food chemistry, 2017Co-Authors: Sudhanshu Saxena, Jyoti Tripathi, Suchandra Chatterjee, Satyendra GautamAbstract:Various samples of raw (unprocessed) floral honey collected from different geographical locations of India were assayed for its antimutagenicity against ethyl methanesulfonate in E. coli MG1655 Cells through rifampicin resistance assay. A monofloral honey (“Pongammia pinnata”, local name “Karanj”) displayed maximum antimutagenicity (78.0 ± 1.7; P ≤ 0.05). Solid phase extraction (using Amberlite XAD-2 resin) followed by HPLC resulted into different peaks displaying varying antimutagenicity. Peak at retention time (Rt) 27.9 min (henceforth called P28) displayed maximum antimutagenicity and was further characterized to be abscisic acid (ABA) using ESI–MS and NMR. Its antimutagenicity was reconfirmed through human Lymphoblast Cell Line (TK6) mutation assay using thymidine kinase (tk+/−) Cell Line. Although ABA from this honey displayed strong antimutagenicity, it lacked any in vitro antioxidant capacity indicating noninvolvement of any radical scavenging in the observed antimutagenicity.
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Development of ambient storable meal for calamity victims and other targets employing radiation processing and evaluation of its nutritional, organoleptic, and safety parameters
LWT - Food Science and Technology, 2016Co-Authors: Sanjeev Kumar, Sudhanshu Saxena, Jyoti Verma, Satyendra GautamAbstract:During natural calamities nutritious, safe, and ambient storable meal is issue of immense concern. Recently Joint FAO/IAEA programme of nuclear techniques in Food and Agriculture has undertaken Coordinated Research Project (CRP) where a product ‘Stuffed Baked Food (SBF)’ was developed in India. SBF consists of partially fermented multigrain dough enriched with 5% saturated fat and stuffed with flour of roasted chick pea; boiled and peeled potato (mashed); and cooked chick pea split (mashed) with spices and salt. Stuffed lobe was convection baked, vacuum packaged and gamma irradiated (15 kGy). The product was well acceptable after prolonged storage at ambient temperature while retaining its quality attributes. SBF can also be useful for other targets like defence personnel, school lunch programme, expeditions, and astronauts. Mutation analyses in models including human TK6 Lymphoblast Cell Line at genes tk+/− and hprt+; and bacterial systems [Escherichia coli MG1655 Cells (rpoB gene); and Ames strains (TA 100 and TA 102)] endorsed its genotoxic safety.
Ana Tereza Ribeiro De Vasconcelos - One of the best experts on this subject based on the ideXlab platform.
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Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencing
Genome biology, 2010Co-Authors: Qi Zhao, Ewen F. Kirkness, Otavia L. Caballero, Pedro A. F. Galante, Raphael B. Parmigiani, Lee Edsall, Samantha Kuan, Samuel Levy, Ana Tereza Ribeiro De VasconcelosAbstract:Background: To identify potential tumor suppressor genes, genome-wide data from exome and transcriptome sequencing were combined to search for genes with loss of heterozygosity and allele-specific expression. The analysis was conducted on the breast cancer Cell Line HCC1954, and a Lymphoblast Cell Line from the same individual, HCC1954BL. Results: By comparing exome sequences from the two Cell Lines, we identified loss of heterozygosity events at 403 genes in HCC1954 and at one gene in HCC1954BL. The combination of exome and transcriptome sequence data also revealed 86 and 50 genes with allele specific expression events in HCC1954 and HCC1954BL, which comprise 5.4% and 2.6% of genes surveyed, respectively. Many of these genes identified by loss of heterozygosity and allelespecific expression are known or putative tumor suppressor genes, such as BRCA1, MSH3 and SETX, which participate in DNA repair pathways. Conclusions: Our results demonstrate that the combined application of high throughput sequencing to exome and allele-specific transcriptome analysis can reveal genes with known tumor suppressor characteristics, and a shortlist of novel candidates for the study of tumor suppressor activities.
David W. Golde - One of the best experts on this subject based on the ideXlab platform.
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Production of erythroid-potentiating activity by a human T-Lymphoblast Cell Line (erythroid colonies/lymphokine/burst-promoting activity/T lymphocytes/colony-stimulating factor)
2016Co-Authors: David W. Golde, Noelle Bersch, Shirley G. Quan, Aldons J. LusisAbstract:We derived a human T-Lymphoblast Cell Line (Mo) that constitutively elaborates certain lymphokines. The Mo Cells produce a colony-stimulating factor necessary for the growth of human granulocyte-monocyte precursors in vitro as well as an erythroid-potentiating activity (EPA) that enhances the proliferation of human erythroid progenitors in vitro. In the presence of serum, the EPA in Mo-conditioned medium stimu- lated the growth of small and large erythroid colonies almost 2-fold. EPA was also produced in serum-free medium, and, when assayed in serum-free cultures of human erythroid progenitors, it stimulated colony growth about 3-fold. The EPA produced by the Mo Cell Line did not stimulate normal murine erythroid progenitors (CFU-E) or Friend erythroleukemia Cell growth in vitro. EPA was inactivated by protease treatment but was re- markably heat stable, with most of the activity recovered after boiling for 15 min. Preliminary biochemical characterization suggests that EPA is an acidic glycoprotein with molecular weight approximately 45,000. EPA is clearly separable from colony-stimulating factor on the basis of heat stability and gel- filtration chromatography. The present observations provide strong support for the concept that activated T Cells produce humoral factors important in the regulation of erythropoiesis. The availability of a Cell Line producing human EPA should facilitate the characterization of the protein and permit defin- itive studies of its biologic effects.
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production of erythroid potentiating activity by a human t Lymphoblast Cell Line erythroid colonies lymphokine burst promoting activity t lymphocytes colony stimulating factor
2016Co-Authors: David W. Golde, Noelle Bersch, Shirley G. Quan, Aldons J. LusisAbstract:We derived a human T-Lymphoblast Cell Line (Mo) that constitutively elaborates certain lymphokines. The Mo Cells produce a colony-stimulating factor necessary for the growth of human granulocyte-monocyte precursors in vitro as well as an erythroid-potentiating activity (EPA) that enhances the proliferation of human erythroid progenitors in vitro. In the presence of serum, the EPA in Mo-conditioned medium stimu- lated the growth of small and large erythroid colonies almost 2-fold. EPA was also produced in serum-free medium, and, when assayed in serum-free cultures of human erythroid progenitors, it stimulated colony growth about 3-fold. The EPA produced by the Mo Cell Line did not stimulate normal murine erythroid progenitors (CFU-E) or Friend erythroleukemia Cell growth in vitro. EPA was inactivated by protease treatment but was re- markably heat stable, with most of the activity recovered after boiling for 15 min. Preliminary biochemical characterization suggests that EPA is an acidic glycoprotein with molecular weight approximately 45,000. EPA is clearly separable from colony-stimulating factor on the basis of heat stability and gel- filtration chromatography. The present observations provide strong support for the concept that activated T Cells produce humoral factors important in the regulation of erythropoiesis. The availability of a Cell Line producing human EPA should facilitate the characterization of the protein and permit defin- itive studies of its biologic effects.
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Growth-promoting actions of parathyroid hormone, adrenocorticotrophic hormone, and thyroid-stimulating hormone: in vitro studies in normal and pygmy T-Lymphoblast Cell Lines.
Pediatric Research, 1995Co-Authors: Mitchell E. Geffner, Noelle Bersch, Alan B. Cortez, Robert C. Bailey, David W. GoldeAbstract:ABSTRACT: We used an in vitroT-Lymphoblast clonal proliferation assay to quantify human IGF-I (hIGF-I)-, human PTH (hPTH)-, human ACTH (hACTH)-, and human TSH (hTSH)-stimulated growth of human T-Cell leukemia virus-II-transformed T-Lymphoblast Cell Lines from normal individuals and to elucidate the role of IGF-I as the mediator of hPTH-, hACTH-, and hTSH-induced T-Cell growth. Normal T-Lymphoblast Cell Lines respond to hIGF-I in a bimodal fashion. The mean first peak response was 143 ± 9.8% above baseLine (defined as 100%) occurring at 8 μg/L, and the mean second peak response was 154 ± 14.4% occurring at 100 μ/L. Both responses were completely blocked after incubation with αIR-3, an MAb to the IGF-I receptor (by analysis of variance, p= 0.015 between full response curves). After stimulation with hPTH, the mean peak clonal response of normal T-Lymphoblast Cell Lines was 189 ± 7.0%; after incubation with αIR-3, the mean peak clonal response was 108 ± 7.9% (p= 0.0015 between full response curves). The mean peak clonal response of normal T-Lymphoblast Cell Lines after hACTH stimulation was 192 ± 8.6%; preincubation with αIR-3 reduced the mean peak clonal response to 94 ± 1.2% (p< 0.0001 between full response curves). With hTSH stimulation, the mean peak clonal response of normal T-Lymphoblast Cell Lines was 167 ± 7.0%; after incubation with αR-3, the mean peak clonal response was 94 ± 8.2% (p= 0.003 between full response curves). After stimulation with hIGF-I, hPTH, hACTH, and hTSH, the mean peak clonal responses of a pygmy T-Lymphoblast Cell Line were 112 ± 8.2, 122 ± 1.5. 99 ± 4.2. and 98 ± 5.5%, respectively (all p≤ 0.0004 between corresponding complete pygmy and normal response curves). These data indicate that hPTH, hACTH, and hTSH stimulate growth of normal human T-Lymphoblast Cell Lines through local action of IGF-I, because these effects can all be blocked by preincubation with MAb against the IGF-I receptor. The pygmy T-Lymphoblast Cell Line showed little or no clonal expansion in the presence of hIGF-1 itself, or in response to hPTH, hACTH, or hTSH, further supporting the notion that an intact IGF-I response mechanism is necessary for the proliferative response to hPTH, hACTH, and hTSH in human T-Lymphoblasts.
Patrick Robberecht - One of the best experts on this subject based on the ideXlab platform.
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Molecular cloning and functional characterization of a human VIP receptor from SUP-T1 Lymphoblasts.
Biochemical and biophysical research communications, 1994Co-Authors: Michal Svoboda, Michèle Tastenoy, J. Vanrampelbergh, J.f. Goossens, P. Deneef, Magali Waelbroeck, Patrick RobberechtAbstract:Abstract We have cloned and sequenced a cDNA isolated from a human SUP-T1 Lymphoblast Cell Line library. It encoded a 457 amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) Cells stably transfected with cloned cDNA expressed a specific binding of 125I[Acetyl-His1]PACAP-27. This binding was inhibited by GTP, and by the peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated adenylate cyclase activity. The order of potency was PACAP-38 > VIP ≥ helodermin ≥ PACAP-27. Comparison of the results in two Cell Lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27.
Jonathan G. Scammell - One of the best experts on this subject based on the ideXlab platform.
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The origin of four squirrel monkey Cell Lines established by karyotype analysis.
Cytogenetics and cell genetics, 2001Co-Authors: Jonathan G. Scammell, J.l. Wright, Cathy M. Tuck-mullerAbstract:The squirrel monkey is a neotropical primate genus which is widely used in biomedical research but includes individual species and subspecies that respond differently to experimental perturbations. GTG-banding patterns of chromosomes 15 and 16, which are distinct among different squirrel monkey species and subspecies, were used to determine the origin of three lung fibroblast Cell Lines from squirrel monkeys of unknown genetic background (DPSO 114/74, SqMkLu/68, and 7603830) and to confirm the origin of a Lymphoblast Cell Line (GSML) recently established from Guyanese squirrel monkey. DPSO 114/74 Cells are from Peruvian squirrel monkey, SqMkLu/68 Cells are Bolivian squirrel monkey, and 7603830 Cells are from a Peruvian/Bolivian hybrid. Chromosome analysis of GSML Cells confirmed that they are from Guyanese squirrel monkey.
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Glucocorticoid-resistant B-Lymphoblast Cell Line derived from the Bolivian squirrel monkey (Saimiri boliviensis boliviensis)
Laboratory animal science, 1998Co-Authors: Philip D. Reynolds, K. P. Roveda, J. A. Tucker, Charleen M. Moore, Donna L. Valentine, Jonathan G. ScammellAbstract:The goal of the study reported here was to develop a continuous Cell Line from the squirrel monkey that expresses the species-specific phenotype of impaired sensitivity to glucocorticoids. Thirty milliliters of blood from a male Bolivian squirrel monkey (Saimiri boliviensis boliviensis) was fractionated, and the buffy coat was obtained and incubated in the presence of B95-8 Cell-conditioned medium, an abundant source of Epstein-Barr virus (EBV), and 2 micrograms of cyclosporin A/ml. Cell growth was detected within 8 weeks, after which the Cells were cloned by use of the limiting dilution method. One clone (4D8) was characterized in detail. The chromosomal count and G-banding pattern confirmed that the Cells were of Bolivian squirrel monkey origin. The B-Cell origin of these Cells was indicated by electron microscopic analysis and was confirmed by expression of CD20. The Cells stained strongly for LMP1, a marker of latent EBV infection, and occasionally for the lytic infection marker ZEBRA (BZLF1). The responsiveness of clone 4D8 Cells to glucocorticoids was determined by comparing the effects of dexamethasone on Cell growth and the induction of a glucocorticoid-inducible mRNA in 4D8 Cells with the effects on a human EBV-transformed B-Lymphoblast Cell Line (HL). Dexamethasone inhibited the growth of HL Cells, with IC50 of approximately 9 nM, but had no effect on the growth of 4D8 Cells. The induction of FK506-binding protein FKBP51 mRNA by dexamethasone was also significantly blunted in 4D8 Cells. Thus, we have developed and characterized a squirrel monkey Lymphoblastic Cell Line derived by transformation of B-lymphocytes with EBV; the Cell Line has diminished growth and transcriptional responses to glucocorticoids.
