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Bertille De Barbeyrac - One of the best experts on this subject based on the ideXlab platform.
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Concern regarding the alleged spread of hypervirulent Lymphogranuloma Venereum chlamydia trachomatis strain in Europe
'European Centre for Disease Control and Prevention (ECDC)', 2020Co-Authors: Seth-smith, Helena M. B., Bertille De Barbeyrac, Galán, Juan C., Goldenberger Daniel, Lewis, David A., Peuchant Olivia, Bébéar Cecile, Bénard Angele, Carter Ian, Kok JenAbstract:A recent surveillance and outbreak report published in Eurosurveillance by Petrovay et al. on the ‘Emergence of the Lymphogranuloma Venereum L2c genovariant, Hungary, 2012 to 2016’ [1] provides an observation of the first European cases of a genotype of Chlamydia trachomatis associated with severe haemorrhagic proctitis. The authors of this paper diagnosed the strains as Lymphogranuloma Venereum (LGV)- associated and performed partial sequencing of the ompA gene (ca 1,070 bp), which is a standard typing method for C. trachomatis. The ompA gene sequence obtained was compared with those from reference isolates, and reported to be 100% concordant with the ompA sequence belonging to an L2-D recombinant strain described in 2011 [2]. This strain was named ‘L2c’, as it was found to possess a chimeric genome, not because it has a novel ompA-genotype. We would like to point out that the ompA gene sequence of this L2-D recombinant strain, and by implication those of the Hungarian isolates, is identical to that of archetypal L2 strains, for example the reference strain L2/434 [3]
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2019 European guideline on the management of Lymphogranuloma Venereum
Journal of the European Academy of Dermatology and Venereology, 2019Co-Authors: H. J. C. De Vries, Bertille De Barbeyrac, N. H. N. De Vrieze, J. D. Viset, J.a. White, M. Vall-mayans, M. UnemoAbstract:New or important issues in this updated version of the 2013 European guideline on the management of Lymphogranuloma Venereum (LGV): Epidemiology Lymphogranuloma Venereum continues to be endemic among European men who have sex with men (MSM) since 2003. Lymphogranuloma Venereum infections in heterosexuals are extremely rare in Europe, and there is no evidence of transmission of LGV in the European heterosexual population. Aetiology and transmission Chlamydia trachomatis serovars/genovars L2b and L2 are the causative strains in the majority of cases in Europe. Clinical features Among MSM, about 25% of the anorectal LGV infections are asymptomatic. Genital infections among MSM are rare; the ratio of genital vs. anorectal LGV infections is 1 in 15. Diagnosis To diagnose LGV, a sample tested C. trachomatis positive with a commercial nucleic acid amplification test (NAAT) platform should be confirmed with an LGV discriminatory NAAT. Treatment Doxycycline 100 mg twice a day orally for 21 days is the recommended treatment for LGV. This same treatment is recommended also in asymptomatic patients and contacts of LGV patients. If another regimen is used, a test of cure (TOC) must be performed.
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Changing Pattern of Chlamydia trachomatis Strains in Lymphogranuloma Venereum Outbreak, France, 2010-2015
Emerging Infectious Diseases, 2016Co-Authors: Olivia Peuchant, Arabella Touati, Clément Sperandio, Nadège Hénin, Cécile Laurier-nadalié, Cecile Bebear, Bertille De BarbeyracAbstract:We describe a change in the molecular epidemiology of Chlamydia trachomatis strains involved in an outbreak of rectal Lymphogranuloma Venereum in France during January 2010 April 2015. Until 2012, the C. trachomatis L2b strain predominated; however, starting in 2013, most cases involved the L2 strain. We also identified 4 genetic L2b ompA variants.
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Changing Pattern of Chlamydia trachomatis Strains in Lymphogranuloma Venereum Outbreak, France, 2010–2015
'Centers for Disease Control and Prevention (CDC)', 2016Co-Authors: Olivia Peuchant, Arabella Touati, Clément Sperandio, Nadège Hénin, Cécile Laurier-nadalié, Cecile Bebear, Bertille De BarbeyracAbstract:We describe a change in the molecular epidemiology of Chlamydia trachomatis strains involved in an outbreak of rectal Lymphogranuloma Venereum in France during January 2010–April 2015. Until 2012, the C. trachomatis L2b strain predominated; however, starting in 2013, most cases involved the L2 strain. We also identified 4 genetic L2b ompA variants
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The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of Lymphogranuloma Venereum is not specific to L2b strains
Clinical Microbiology and Infection, 2016Co-Authors: Arabella Touati, Olivia Peuchant, Nadège Hénin, Cecile Bebear, Bertille De BarbeyracAbstract:The French Reference Centre for chlamydiae uses two real-time PCRs targeting the pmpH gene of Chlamydia trachomatis to differentiate between L strains and variant L2b, responsible for a Lymphogranuloma Venereum outbreak in Europe. We compared the results obtained for 122 L2b C. trachomatis-positive specimens, using the two real-time PCRs, with the sequencing of the ompA gene. Only 91 specimens were confirmed as L2b. Our results demonstrate that the Lymphogranuloma Venereum outbreak is no longer dominated by the variant L2b, and that many L-positive specimens were misidentified as L2b with the method used, which raises the question of its specificity.
Henry J C De Vries - One of the best experts on this subject based on the ideXlab platform.
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low prevalence of urethral Lymphogranuloma Venereum infections among men who have sex with men a prospective observational study sexually transmitted infection clinic in amsterdam the netherlands
Sexually Transmitted Diseases, 2017Co-Authors: Nynke Hesselina Neeltje De Vrieze, Martijn S Van Rooijen, Bart Versteeg, Sylvia M Bruisten, Jannie J Van Der Helm, Henry J C De VriesAbstract:In contrast to anorectal Lymphogranuloma Venereum (LGV), few urogenital LGV cases are reported in men who have sex with men. Lymphogranuloma Venereum was diagnosed in 0.06% (7/12,174) urine samples, and 0.9% (109/12,174) anorectal samples. Genital-anal transmission seems unlikely the only mode of transmission. Other modes like oral-anal transmission should be considered.
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pitfalls in the diagnosis and management of inguinal Lymphogranuloma Venereum important lessons from a case series
Sexually Transmitted Infections, 2014Co-Authors: Emerentiana Veronica Oud, Nynke Hesselina Neeltje De Vrieze, Arjan De Meij, Henry J C De VriesAbstract:Current Lymphogranuloma Venereum (LGV) guidelines mainly focus on anorectal infections. Inguinal LGV infections have been rare in the current epidemic among men who have sex with men (MSM), but might require a different approach not yet recommended in current guidelines for the treatment and diagnosis of LGV. We describe 4 inguinal LGV cases. Three MSM developed inguinal LGV infection several weeks after a previous consultation, of which two had received azithromycin after being notified for LGV. Three failed the recommended 21 days doxycycline treatment. These inguinal LGV cases highlight 3 pitfalls in the current standard management of LGV: (1) Urethral chlamydia infections in MSM can be caused by LGV biovars that in contrast to non-LGV biovars require prolonged antibiotic therapy. (2) The recommended one gram azithromycin contact treatment seems insufficient to prevent established infections. (3) Inguinal LGV may require prolonged courses of doxycycline, exceeding the currently advised 21 days regimen.
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Lymphogranuloma Venereum among men who have sex with men an epidemiological and clinical review
Expert Review of Anti-infective Therapy, 2014Co-Authors: Nynke Hesselina Neeltje De Vrieze, Henry J C De VriesAbstract:Lymphogranuloma Venereum (LGV) is a sexually transmitted infection, previously only seen in tropical regions. This changed in 2003 when the first endemically acquired LGV cases were reported in Rotterdam, the Netherlands, among predominantly HIV positive men who have sex with men (MSM). Early diagnosis is important to prevent irreversible complications and to stop further transmission in the community. In contrast to earlier reports, approximately 25% of LGV infections are asymptomatic and form an easily missed undetected reservoir. The majority of reported infections in MSM are found in the anorectal canal and not urogenital, which leaves the mode of transmission within the MSM network unclear. Given the increasing trend, the LGV endemic is clearly not under control. Therefore directed screening must be intensified.
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urethral Lymphogranuloma Venereum infections in men with anorectal Lymphogranuloma Venereum and their partners the missing link in the current epidemic
Sexually Transmitted Diseases, 2013Co-Authors: Nynke Hesselina Neeltje De Vrieze, Arjen G C L Speksnijder, Martijn S Van Rooijen, Henry J C De VriesAbstract:Urethral Lymphogranuloma Venereum (LGV) is not screened routinely. We found that in 341 men having sex with men with anorectal LGV, 7 (2.1%) had concurrent urethral LGV. Among 59 partners, 4 (6.8%) had urethral LGV infections. Urethral LGV is common, probably key in transmission, and missed in current routine LGV screening algorithms.
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typing of Lymphogranuloma Venereum chlamydia trachomatis strains
Emerging Infectious Diseases, 2010Co-Authors: Linus Christerson, Henry J C De Vries, Marti Vallmayans, Bertille De Barbeyrac, Julius Schachter, Charlotte A Gaydos, Birgit Henrich, Steen Hoffmann, Johannes Thorvaldsen, Markus KlintAbstract:We analyzed by multilocus sequence typing 77 Lymphogranuloma Venereum Chlamydia trachomatis strains from men who have sex with men in Europe and the United States. Specimens from an outbreak in 2003 in Europe were monoclonal. In contrast, several strains were in the United States in the 1980s, including a variant from Europe.
Catherine A Ison - One of the best experts on this subject based on the ideXlab platform.
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asymptomatic Lymphogranuloma Venereum in men who have sex with men united kingdom
Emerging Infectious Diseases, 2016Co-Authors: Cara Saxon, Gwenda Hughes, Catherine A IsonAbstract:We investigated prevalence of Lymphogranuloma Venereum (LGV) among men who have sex with men who were tested for chlamydia at 12 clinics in the United Kingdom during 10 weeks in 2012. Of 713 men positive for Chlamydia trachomatis, 66 (9%) had LGV serovars; 15 (27%) of 55 for whom data were available were asymptomatic.
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a real time quadriplex pcr assay for the diagnosis of rectal Lymphogranuloma Venereum and non Lymphogranuloma Venereum chlamydia trachomatis infections
Sexually Transmitted Infections, 2008Co-Authors: Chengyen Chen, Sarah Alexander, Kai-hua Chi, Catherine A Ison, Ronald C BallardAbstract:Objectives: To develop and evaluate a real-time quadriplex PCR for the diagnosis of Lymphogranuloma Venereum (LGV) and non-LGV chlamydial infections using rectal swab specimens. Methods: The design of the real-time quadriplex PCR assay incorporates an LGV-specific, a non-LGV-specific target sequence, a Chlamydia trachomatis plasmid target, and the human RNase P gene as an internal control. The performance of the quadriplex PCR was compared with a previously reported real-time duplex PCR assay on which LGV diagnosis was based on exclusion. Results: Very good agreement (85 of 89 specimens, 95.5%) was found between the two multiplex PCR assays for the detection of C trachomatis DNA (kappa value 0.93, 95% CI 0.86 to 0.99). Both assays identified 34 LGV, 35 non-LGV C trachomatis and 16 negative specimens. Of two specimens that tested positive for non-LGV by the duplex PCR, one was found to be a mixed infection and the other was positive only for plasmid and RNase P targets by the quadriplex PCR. Two additional specimens that had equivocal results for non-LGV by the duplex PCR also tested positive only for plasmid target and human DNA by the quadriplex PCR. In addition, six specimens that tested negative by the duplex PCR assay were found to be invalid when using the quadriplex PCR. Conclusions: A real-time quadriplex PCR assay has been developed that is capable of detecting LGV, non-LGV, or mixed infections simultaneously in rectal specimens. The assay also contains a supplemental amplification target for the confirmation of C trachomatis infection as well as a human DNA control for monitoring sample adequacy and PCR inhibition.
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update on Lymphogranuloma Venereum in the united kingdom
Sexually Transmitted Infections, 2007Co-Authors: Heather Jebbari, John White, Sarah Alexander, Helen Ward, Patrick French, Gillian Dean, Barry Evans, Maria Solomou, A Thornton, Catherine A IsonAbstract:Objectives: This report updates the UK epidemiology of Lymphogranuloma Venereum (LGV) to the end of April 2007. Methods: The Health Protection Agency’s Centre for Infections undertakes laboratory testing for LGV and subsequent epidemiological investigation of cases after laboratory confirmation of the LGV serovars (L1–3). Data analysis of enhanced surveillance and laboratory reports was undertaken. Results: From October 2004 to end April 2007, 492 cases of LGV have been diagnosed and enhanced surveillance forms have been returned for 423. Cases peaked in the third quarter of 2005 with an average of 32 cases per month, while in 2006 this fell to 12 cases per month. Nationally, the outbreak is focused in London, Brighton and the North West. All cases are in men, 99% of whom are MSM, with a median age of 40 and predominantly white ethnicity (91%). Co-infection remains considerable: HIV (74%); hepatitis C (14%); syphilis (5%); and other STIs including gonorrhoea, genital herpes and hepatitis B. The number of men reporting greater than 10 sexual contacts in the previous 3 months has reduced from 23% (47) to 13% (15) from 2005–2006. Discussion: The epidemic continues in the mostly white MSM population of the UK. The demographics of LGV remain similar to those previously described and high levels of HIV co-infection continue. Reduced numbers of sexual contacts might be contributing to the reduced numbers of LGV seen in 2006 but could simply mean that LGV is moving out of the highest risk groups.
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Lymphogranuloma Venereum in the united kingdom
Clinical Infectious Diseases, 2007Co-Authors: Helen Ward, Sarah Alexander, N Macdonald, Iona M C Martin, Ian Simms, Patrick French, Kevin A Fenton, Gillian Dean, Catherine A IsonAbstract:Background. Over the past 2 years, Lymphogranuloma Venereum (LGV), caused by L serovars of Chlamydia trachomatis, has emerged as a significant problem among men who have sex with men (MSM). We report on, to our knowledge, the largest case series of LGV to date, with detailed epidemiological and clinical characteristics of the epidemic in the United Kingdom. Methods. A national diagnostic service and surveillance system was established in October 2004. Cases were confirmed by the presence of C. trachomatis and an LGV serovar (L1, L2, or L3) from genotyping. For confirmed cases, an enhanced surveillance questionnaire was sent to the clinician. Results. Through February 2006, a total of 327 cases of LGV were confirmed. Cases were diagnosed across the United Kingdom, with the majority from London (71%) and Brighton (13%). Case reports were received for 282 MSM. The majority (96%) had proctitis, many with severe local and systemic symptoms. There was a high level of coinfection with human immunodeficiency virus (76%), hepatitis C (19%), and other sexually transmitted infections (39%). Nine cases of human immunodeficiency virus infection were diagnosed around the same time as LGV. Most cases were acquired within the United Kingdom, although patients with early cases were more likely to report contacts in The Netherlands. Conclusions. We found a significant burden of this once-rare sexually transmitted infection among MSM in the United Kingdom. LGV may be contributing to the epidemic of human immunodeficiency virus infection by facilitating transmission. Further control efforts are required, including awareness campaigns, continued detailed surveillance, and expanded chlamydia testing among MSM.
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diagnosis of Lymphogranuloma Venereum from biopsy samples
Gut, 2006Co-Authors: Iona M C Martin, N Macdonald, Catherine A Ison, S A Alexander, K Mccarthy, Helen WardAbstract:The Health Protection Agency Centre for Infections launched an alert in October 2004 to improve the awareness, diagnosis, and control of Lymphogranuloma Venereum (LGV), a sexually transmitted chlamydial infection, following a series of outbreaks in Western Europe.1 To date (9/3/2006), 334 cases of LGV have been diagnosed in 334 men. The case definition for a confirmed case of LGV is the presence of C trachomatis specific DNA, using two nucleic acid amplification tests (NAATs) with different primers, of serovars L1, L2, or L3, determined by genotyping (http://www.hpa.org.uk/infections/topics\_az/hiv\_and_sti/LGV/lgv.htm). All cases of LGV to date in the UK have been in men who have sex with men and typically present with proctitis and/or inguinal lymphadenopathy. Some of the men in the UK diagnosed with LGV reported long duration of symptoms presenting to gastroenterologists and having been wrongly diagnosed with inflammatory …
Sarah Alexander - One of the best experts on this subject based on the ideXlab platform.
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rapid increase in Lymphogranuloma Venereum in men who have sex with men united kingdom 2003 to september 2015
Eurosurveillance, 2015Co-Authors: Tristan Childs, Sarah Alexander, Ian Simms, Gwenda Hughes, Kirstine Eastick, Nigel FieldAbstract:United Kingdom (UK) national data show a sharp increase in diagnoses of Lymphogranuloma Venereum (LGV) since 2012. Most cases are in men who have sex with men (MSM) living in London, with high rates of co-infection with HIV and other sexually transmitted infections. In light of these data, and the recent finding that one quarter of LGV infections may be asymptomatic, clinicians should be vigilant in testing for LGV, including in asymptomatic HIV-positive MSM.
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Lymphogranuloma Venereum proctitis masquerading as inflammatory bowel disease in 12 homosexual men.
Alimentary pharmacology & therapeutics, 2010Co-Authors: S. Soni, R. Srirajaskanthan, Sebastian Lucas, Sarah Alexander, Terry Wong, John WhiteAbstract:Aliment Pharmacol Ther 2010; 32: 59–65 Summary Background Lymphogranuloma Venereum (LGV) is a recognized cause of proctitis. Symptoms, endoscopy and histology findings are similar in IBD and LGV proctitis. Aims To characterize the clinical, endoscopic and histological features seen in men diagnosed initially with IBD and subsequently with LGV proctitis, and to attempt isolation of Chlamydia trachomatis DNA from the stored rectal biopsy specimens of these patients using real-time PCR. Methods Clinical data were collated from confirmed or suspected cases of LGV proctitis where endoscopy and biopsy had been performed as part of the investigation of clinical symptoms. LGV was confirmed by the detection of LGV-specific DNA from rectal swab specimens, with supportive evidence from Chlamydial serology. Stored histological specimens from rectal biopsies were analysed retrospectively for LGV-specific DNA with molecular techniques. Results Rectal biopsies had been obtained from twelve cases of LGV proctitis. Mucosal ulcers, cryptitis, crypt abscesses and granulomas were common histological findings. Extraction of LGV-specific DNA from rectal biopsy specimens enabled confirmation of three suspected cases. Conclusions During the recent LGV proctitis epidemic among UK men who have sex with men, it has become apparent that this infection may closely resemble IBD. Gastroenterologists should remain alert to LGV as a cause of proctitis in this group.
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the prevalence of Lymphogranuloma Venereum infection in men who have sex with men results of a multicentre case finding study
Sexually Transmitted Infections, 2009Co-Authors: Helen Ward, Sarah Alexander, Patrick French, Gillian Dean, C Carder, Dan Ivens, Clare Ling, John Paul, William Tong, John WhiteAbstract:Objective: To determine the prevalence of Lymphogranuloma Venereum (LGV) and non-LGV associated serovars of urethral and rectal Chlamydia trachomatis (CT) infection in men who have sex with men (MSM). Design: Multicentre cross-sectional survey. Setting: Four genitourinary medicine clinics in the United Kingdom from 2006–7. Subjects: 4825 urethral and 6778 rectal samples from consecutive MSM attending for sexual health screening. Methods: Urethral swabs or urine and rectal swabs were tested for CT using standard nucleic acid amplification tests. Chlamydia-positive specimens were sent to the reference laboratory for serovar determination. Main outcome: Positivity for both LGV and non-LGV associated CT serovars; proportion of cases that were symptomatic. Results: The positivity (with 95% confidence intervals) in rectal samples was 6.06% (5.51% to 6.66%) for non-LGV CT and 0.90% (0.69% to 1.16%) for LGV; for urethral samples 3.21% (2.74% to 3.76%) for non-LGV CT and 0.04% (0.01% to 0.16%) for LGV. The majority of LGV was symptomatic (95% of rectal, one of two urethral cases); non-LGV chlamydia was mostly symptomatic in the urethra (68%) but not in the rectum (16%). Conclusions: Chlamydial infections are common in MSM attending for sexual health screening, and the majority are non-LGV associated serovars. We did not identify a large reservoir of asymptomatic LGV in the rectum or urethra. Testing for chlamydia from the rectum and urethra should be included for MSM requesting a sexual health screen, but serovar-typing is not indicated in the absence of symptoms. We have yet to identify the source of most cases of LGV in the UK. Lymphogranuloma Venereum (LGV) has reemerged as a significant sexually transmitted infection (STI) among men who have sex with men (MSM) in the UK and Europe. Initial outbreaks and clusters of cases in The Netherlands, Germany, UK and France have been followed by reports in several other parts of Europe, North America and Australia. 1
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a real time quadriplex pcr assay for the diagnosis of rectal Lymphogranuloma Venereum and non Lymphogranuloma Venereum chlamydia trachomatis infections
Sexually Transmitted Infections, 2008Co-Authors: Chengyen Chen, Sarah Alexander, Kai-hua Chi, Catherine A Ison, Ronald C BallardAbstract:Objectives: To develop and evaluate a real-time quadriplex PCR for the diagnosis of Lymphogranuloma Venereum (LGV) and non-LGV chlamydial infections using rectal swab specimens. Methods: The design of the real-time quadriplex PCR assay incorporates an LGV-specific, a non-LGV-specific target sequence, a Chlamydia trachomatis plasmid target, and the human RNase P gene as an internal control. The performance of the quadriplex PCR was compared with a previously reported real-time duplex PCR assay on which LGV diagnosis was based on exclusion. Results: Very good agreement (85 of 89 specimens, 95.5%) was found between the two multiplex PCR assays for the detection of C trachomatis DNA (kappa value 0.93, 95% CI 0.86 to 0.99). Both assays identified 34 LGV, 35 non-LGV C trachomatis and 16 negative specimens. Of two specimens that tested positive for non-LGV by the duplex PCR, one was found to be a mixed infection and the other was positive only for plasmid and RNase P targets by the quadriplex PCR. Two additional specimens that had equivocal results for non-LGV by the duplex PCR also tested positive only for plasmid target and human DNA by the quadriplex PCR. In addition, six specimens that tested negative by the duplex PCR assay were found to be invalid when using the quadriplex PCR. Conclusions: A real-time quadriplex PCR assay has been developed that is capable of detecting LGV, non-LGV, or mixed infections simultaneously in rectal specimens. The assay also contains a supplemental amplification target for the confirmation of C trachomatis infection as well as a human DNA control for monitoring sample adequacy and PCR inhibition.
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update on Lymphogranuloma Venereum in the united kingdom
Sexually Transmitted Infections, 2007Co-Authors: Heather Jebbari, John White, Sarah Alexander, Helen Ward, Patrick French, Gillian Dean, Barry Evans, Maria Solomou, A Thornton, Catherine A IsonAbstract:Objectives: This report updates the UK epidemiology of Lymphogranuloma Venereum (LGV) to the end of April 2007. Methods: The Health Protection Agency’s Centre for Infections undertakes laboratory testing for LGV and subsequent epidemiological investigation of cases after laboratory confirmation of the LGV serovars (L1–3). Data analysis of enhanced surveillance and laboratory reports was undertaken. Results: From October 2004 to end April 2007, 492 cases of LGV have been diagnosed and enhanced surveillance forms have been returned for 423. Cases peaked in the third quarter of 2005 with an average of 32 cases per month, while in 2006 this fell to 12 cases per month. Nationally, the outbreak is focused in London, Brighton and the North West. All cases are in men, 99% of whom are MSM, with a median age of 40 and predominantly white ethnicity (91%). Co-infection remains considerable: HIV (74%); hepatitis C (14%); syphilis (5%); and other STIs including gonorrhoea, genital herpes and hepatitis B. The number of men reporting greater than 10 sexual contacts in the previous 3 months has reduced from 23% (47) to 13% (15) from 2005–2006. Discussion: The epidemic continues in the mostly white MSM population of the UK. The demographics of LGV remain similar to those previously described and high levels of HIV co-infection continue. Reduced numbers of sexual contacts might be contributing to the reduced numbers of LGV seen in 2006 but could simply mean that LGV is moving out of the highest risk groups.
Birgit Henrich - One of the best experts on this subject based on the ideXlab platform.
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Lymphogranuloma Venereum in men screened for pharyngeal and rectal infection germany
Emerging Infectious Diseases, 2013Co-Authors: K Haar, Birgit Henrich, Sandra Dudarevavizule, Hilmar Wisplinghoff, Fabian Wisplinghoff, Andrea Sailer, Klaus Jansen, Ulrich MarcusAbstract:To determine prevalence of Lymphogranuloma Venereum among men who have sex with men in Germany, we conducted a multicenter study during 2009-2010 and found high rates of rectal and pharyngeal infection in men positive for the causative agent, Chlamydia trachomatis. Many infections were asymptomatic. An adjusted C. trachomatis screening policy is justified in Germany.
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typing of Lymphogranuloma Venereum chlamydia trachomatis strains
Emerging Infectious Diseases, 2010Co-Authors: Linus Christerson, Henry J C De Vries, Marti Vallmayans, Bertille De Barbeyrac, Julius Schachter, Charlotte A Gaydos, Birgit Henrich, Steen Hoffmann, Johannes Thorvaldsen, Markus KlintAbstract:We analyzed by multilocus sequence typing 77 Lymphogranuloma Venereum Chlamydia trachomatis strains from men who have sex with men in Europe and the United States. Specimens from an outbreak in 2003 in Europe were monoclonal. In contrast, several strains were in the United States in the 1980s, including a variant from Europe.
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rapid detection of chlamydia trachomatis and typing of the Lymphogranuloma Venereum associated l serovars by taqman pcr
BMC Infectious Diseases, 2008Co-Authors: Anke Schaeffer, Birgit HenrichAbstract:Infection due to Chlamydia trachomatis is the most common sexually transmitted bacterial disease of global health significance, and especially the L-serovars causing Lymphogranuloma Venereum are increasingly being found in Europe in men who have sex with men. The design and evaluation of a rapid, multiplex, real-time PCR targeting the major outer membrane protein (omp-1) -gene and a L-serovar-specific region of the polymorphic protein H (pmp-H) -gene for the detection of Chlamydia trachomatis is reported here. The PCR takes place as a single reaction with an internal control. For L1-, L2- and L3-serovar differentiation a second set of real-time PCRs was evaluated based on the amplification of serovar-specific omp-1-regions. The detection limit of each real-time PCR, multiplexed or not, was 50 genome copies per reaction with an efficiency ranging from 90,5–95,2%. In a retrospective analysis of 50 ocular, rectal and urogenital specimens formerly tested to be positive for C. trachomatis we identified six L2-serovars in rectal specimens of HIV-positive men, one in a double-infection with L3, and one L2 in a urethral specimen of an HIV-negative male. This unique real-time PCR is specific and convenient for the rapid routine-diagnostic detection of Lymphogranuloma Venereum-associated L-serovars and enables the subsequent differentiation of L1, L2 and L3 for epidemiologic studies.
