The Experts below are selected from a list of 40269 Experts worldwide ranked by ideXlab platform
Tobias Junt - One of the best experts on this subject based on the ideXlab platform.
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restoration of Lymphoid Organ integrity through the interaction of Lymphoid tissue inducer cells with stroma of the t cell zone
Nature Immunology, 2008Co-Authors: Elke Scandella, Sanjiv A. Luther, Beatrice Bolinger, Evelyn Lattmann, Simone Miller, Stephanie Favre, Dan R Littman, Daniela Finke, Tobias JuntAbstract:Restoration of Lymphoid Organ integrity through the interaction of Lymphoid tissue–inducer cells with stroma of the T cell zone
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restoration of Lymphoid Organ integrity through the interaction of Lymphoid tissue inducer cells with stroma of the t cell zone
Nature Immunology, 2008Co-Authors: Elke Scandella, Sanjiv A. Luther, Beatrice Bolinger, Evelyn Lattmann, Simone Miller, Stephanie Favre, Dan R Littman, Daniela Finke, Tobias JuntAbstract:The generation of Lymphoid microenvironments in early life depends on the interaction of Lymphoid tissue-inducer cells with stromal Lymphoid tissue-Organizer cells. Whether this cellular interface stays operational in adult secondary Lymphoid Organs has remained elusive. We show here that during acute infection with lymphocytic choriomeningitis virus, antiviral cytotoxic T cells destroyed infected T cell zone stromal cells, which led to profound disruption of secondary Lymphoid Organ integrity. Furthermore, the ability of the host to respond to secondary antigens was lost. Restoration of the Lymphoid microanatomy was dependent on the proliferative accumulation of Lymphoid tissue-inducer cells in secondary Lymphoid Organs during the acute phase of infection and lymphotoxin alpha(1)beta(2) signaling. Thus, crosstalk between Lymphoid tissue-inducer cells and stromal cells is reactivated in adults to maintain secondary Lymphoid Organ integrity and thereby contributes to the preservation of immunocompetence.
Hye-kyung Park - One of the best experts on this subject based on the ideXlab platform.
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A synthetic cannabinoid JWH-210 reduces Lymphoid Organ weights and T-cell activator levels in mice via CB2 receptors
Naunyn-Schmiedeberg's Archives of Pharmacology, 2017Co-Authors: Sun Mi Gu, Tac-hyung Lee, Jisoon Shin, Hye-kyung Park, Yun Jeong Song, Kyoung Moon Han, Young Hoon Kim, Hyung-soo Kim, Hyun-jin Lee, Hye Jin ChaAbstract:The problem of new psychoactive substances (NPS) is emerging globally. However, the immunotoxicity of synthetic cannabinoids is not evaluated extensively yet. The purpose of the present study was to investigate whether synthetic cannabinoids (JWH-210 and JWH-030) induce adverse effects on Lymphoid Organs, viability of splenocytes and thymocytes, and immune cell activator and cytokines in mice. JWH-210 (10 mg/kg, 3 days, i.p.) is more likely to have cytotoxicity and reduce Lymphoid Organ weight than JWH-030 of ICR mice in vivo. We also demonstrated that JWH-210 administration resulted in the decrease of expression levels of T-cell activator including Cd3e, Cd3g, Cd74p31, and Cd74p41, while JWH-030 increased Cd3g levels. In addition, JWH-210 reduced expression levels of cytokines, such as interleukin-3, interleukin-5, and interleukin-6. Furthermore, we demonstrated that a CB2 receptor antagonist, AM630 inhibited JWH-210-induced cytotoxicity, whereas a CB1 receptor antagonist, rimonabant did not in primary cultured splenocytes. These results suggest that JWH-210 has a cytotoxicity via CB2 receptor action and results in decrement of Lymphoid Organ weights, T-cell activator, and cytokine mRNA expression levels.
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A synthetic cannabinoid JWH-210 reduces Lymphoid Organ weights and T-cell activator levels in mice via CB_2 receptors
Naunyn-Schmiedeberg's Archives of Pharmacology, 2017Co-Authors: Sun Mi Gu, Jisoon Shin, Yun Jeong Song, Hye-kyung ParkAbstract:The problem of new psychoactive substances (NPS) is emerging globally. However, the immunotoxicity of synthetic cannabinoids is not evaluated extensively yet. The purpose of the present study was to investigate whether synthetic cannabinoids (JWH-210 and JWH-030) induce adverse effects on Lymphoid Organs, viability of splenocytes and thymocytes, and immune cell activator and cytokines in mice. JWH-210 (10 mg/kg, 3 days, i.p.) is more likely to have cytotoxicity and reduce Lymphoid Organ weight than JWH-030 of ICR mice in vivo. We also demonstrated that JWH-210 administration resulted in the decrease of expression levels of T-cell activator including Cd3e, Cd3g, Cd74p31, and Cd74p41, while JWH-030 increased Cd3g levels. In addition, JWH-210 reduced expression levels of cytokines, such as interleukin-3, interleukin-5, and interleukin-6. Furthermore, we demonstrated that a CB_2 receptor antagonist, AM630 inhibited JWH-210-induced cytotoxicity, whereas a CB_1 receptor antagonist, rimonabant did not in primary cultured splenocytes. These results suggest that JWH-210 has a cytotoxicity via CB_2 receptor action and results in decrement of Lymphoid Organ weights, T-cell activator, and cytokine mRNA expression levels.
Sun Mi Gu - One of the best experts on this subject based on the ideXlab platform.
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A synthetic cannabinoid JWH-210 reduces Lymphoid Organ weights and T-cell activator levels in mice via CB2 receptors
Naunyn-Schmiedeberg's Archives of Pharmacology, 2017Co-Authors: Sun Mi Gu, Tac-hyung Lee, Jisoon Shin, Hye-kyung Park, Yun Jeong Song, Kyoung Moon Han, Young Hoon Kim, Hyung-soo Kim, Hyun-jin Lee, Hye Jin ChaAbstract:The problem of new psychoactive substances (NPS) is emerging globally. However, the immunotoxicity of synthetic cannabinoids is not evaluated extensively yet. The purpose of the present study was to investigate whether synthetic cannabinoids (JWH-210 and JWH-030) induce adverse effects on Lymphoid Organs, viability of splenocytes and thymocytes, and immune cell activator and cytokines in mice. JWH-210 (10 mg/kg, 3 days, i.p.) is more likely to have cytotoxicity and reduce Lymphoid Organ weight than JWH-030 of ICR mice in vivo. We also demonstrated that JWH-210 administration resulted in the decrease of expression levels of T-cell activator including Cd3e, Cd3g, Cd74p31, and Cd74p41, while JWH-030 increased Cd3g levels. In addition, JWH-210 reduced expression levels of cytokines, such as interleukin-3, interleukin-5, and interleukin-6. Furthermore, we demonstrated that a CB2 receptor antagonist, AM630 inhibited JWH-210-induced cytotoxicity, whereas a CB1 receptor antagonist, rimonabant did not in primary cultured splenocytes. These results suggest that JWH-210 has a cytotoxicity via CB2 receptor action and results in decrement of Lymphoid Organ weights, T-cell activator, and cytokine mRNA expression levels.
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A synthetic cannabinoid JWH-210 reduces Lymphoid Organ weights and T-cell activator levels in mice via CB_2 receptors
Naunyn-Schmiedeberg's Archives of Pharmacology, 2017Co-Authors: Sun Mi Gu, Jisoon Shin, Yun Jeong Song, Hye-kyung ParkAbstract:The problem of new psychoactive substances (NPS) is emerging globally. However, the immunotoxicity of synthetic cannabinoids is not evaluated extensively yet. The purpose of the present study was to investigate whether synthetic cannabinoids (JWH-210 and JWH-030) induce adverse effects on Lymphoid Organs, viability of splenocytes and thymocytes, and immune cell activator and cytokines in mice. JWH-210 (10 mg/kg, 3 days, i.p.) is more likely to have cytotoxicity and reduce Lymphoid Organ weight than JWH-030 of ICR mice in vivo. We also demonstrated that JWH-210 administration resulted in the decrease of expression levels of T-cell activator including Cd3e, Cd3g, Cd74p31, and Cd74p41, while JWH-030 increased Cd3g levels. In addition, JWH-210 reduced expression levels of cytokines, such as interleukin-3, interleukin-5, and interleukin-6. Furthermore, we demonstrated that a CB_2 receptor antagonist, AM630 inhibited JWH-210-induced cytotoxicity, whereas a CB_1 receptor antagonist, rimonabant did not in primary cultured splenocytes. These results suggest that JWH-210 has a cytotoxicity via CB_2 receptor action and results in decrement of Lymphoid Organ weights, T-cell activator, and cytokine mRNA expression levels.
Jason G Cyster - One of the best experts on this subject based on the ideXlab platform.
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The Journal of Experimental Medicine CORRESPONDENCE
2013Co-Authors: Richard L Proia, Jason G CysterAbstract:Cyclical modulation of sphingosine-1phosphate receptor 1 surface expression during lymphocyte recirculation and relationship to Lymphoid Organ transi
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plasma cell s1p1 expression determines secondary Lymphoid Organ retention versus bone marrow tropism
Journal of Experimental Medicine, 2006Co-Authors: Kenji Kabashima, Richard L Proia, Nicole M Haynes, Stephen L Nutt, Maria L Allende, Jason G CysterAbstract:After induction in secondary Lymphoid Organs, a subset of antibody-secreting cells (ASCs) homes to the bone marrow (BM) and contributes to long-term antibody production. The factors determining secondary Lymphoid Organ residence versus BM tropism have been unclear. Here we demonstrate that in mice treated with FTY720 or that lack sphingosine-1-phosphate (S1P) receptor-1 (S1P1) in B cells, IgG ASCs are induced and localize normally in secondary Lymphoid Organs but they are reduced in numbers in blood and BM. Many IgG ASCs home to BM on day 3 of the secondary response and day 3 splenic ASCs exhibit S1P responsiveness, whereas the cells remaining at day 5 are unable to respond. S1P1 mRNA abundance is higher in ASCs isolated from blood compared to spleen, whereas CXCR4 expression is lower. Blood ASCs also express higher amounts of Kruppel-like factor (KLF)2, a regulator of S1P1 gene expression. These findings establish an essential role for S1P1 in IgG plasma cell homing and they suggest that differential regulation of S1P1 expression in differentiating plasma cells may determine whether they remain in secondary Lymphoid Organs or home to BM.
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cyclical modulation of sphingosine 1 phosphate receptor 1 surface expression during lymphocyte recirculation and relationship to Lymphoid Organ transit
Journal of Experimental Medicine, 2005Co-Authors: Charles G Lo, Richard L Proia, Ying Xu, Jason G CysterAbstract:Sphingosine-1-phosphate receptor 1 (S1P 1 ) was recently shown to be required for lymphocyte egress from Lymphoid Organs. Here we have examined the relationship between S1P 1 abundance on the cell and egress efficiency. Using an integrin neutralization approach to separate the processes of entry and exit, we show that pertussis toxin treatment reduces lymphocyte egress from lymph nodes. Retrovirally mediated S1P 1 overexpression is sufficient to reduce B cell accumulation in the splenic white pulp and to promote egress of activated T cells from lymph nodes, whereas S1P 1 +/ − cells have reduced lymph node exit efficiency. Furthermore, lymphocyte S1P 1 is down-regulated in the blood, up-regulated in Lymphoid Organs, and down-regulated again in the lymph. We propose that cyclical ligand-induced modulation of S1P 1 on circulating lymphocytes contributes to establishing their Lymphoid Organ transit time.
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Lymphoid Organ development and cell migration.
Immunological reviews, 2003Co-Authors: Jason G CysterAbstract:As we travel into a new century, confronted with new infectious diseases and bioweapon threats, with surgeons continuing to push the boundaries of what is transplantable, and with gene therapists working on ways to remedy a myriad of genetic diseases, the need for improved methods to augment and suppress immune function is paramount. The recent discovery that a novel immunosuppressant works by blocking lymphocyte egress from Lymphoid Organs provides a compelling example of how improved understanding of Lymphoid Organ function will contribute to future drug development and human health. This volume brings together reviews from leaders in the field of thymus and secondary Lymphoid Organ biology, including discussions on the roles of transcriptional regulators Foxn1, retinoid-related orphan receptor gamma and nuclear factor-kappaB in Lymphoid Organ development, the function of lymphotoxin and other cytokines in Lymphoid tissue Organization, the guidance activity of chemokines in a multitude of immune cell-positioning events, the mechanism of action of the immunosuppressant FTY720, and the application of two-photon laser scanning microscopy to reveal the dynamic behavior of Lymphoid cells in the depths of these essential tissues.
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membrane bound tnf supports secondary Lymphoid Organ structure but is subservient to secreted tnf in driving autoimmune inflammation
Immunity, 2001Co-Authors: Sigrid Ruuls, Robert M Hoek, Vu N Ngo, Tom Mcneil, Linda Lucian, Mary J Janatpour, Heinrich Korner, Heleen Scheerens, Edith M Hessel, Jason G CysterAbstract:Mice without secreted TNF but with functional, normally regulated and expressed membrane-bound TNF (memTNF(Delta/Delta) mice) were created by knocking-in the uncleavable Delta 1-9,K11E TNF allele. In contrast to TNF-deficient mice (TNF(-/-)), memTNF supported many features of Lymphoid Organ structure, except generation of primary B cell follicles. Splenic chemokine expression was near normal. MemTNF-induced apoptosis was mediated through both TNF-R1 and TNF-R2. That memTNF is suboptimal for development of inflammation was revealed in experimental autoimmune encephalomyelitis. Disease severity was reduced in memTNF(Delta/Delta) mice relative to wild-type mice, and the nature of spinal cord infiltrates resembled that in TNF(-/-) mice. We conclude that memTNF supports many processes underlying Lymphoid tissue structure, but secreted TNF is needed for optimal inflammatory lesion development.
Hans Nauwynck - One of the best experts on this subject based on the ideXlab platform.
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virus replication cycle of white spot syndrome virus in secondary cell cultures from the Lymphoid Organ of litopenaeus vannamei
Journal of General Virology, 2015Co-Authors: Lowiese Desmarets, Peter Bossier, Khuong Van Thuong, Vo Van Tuan, Gaetan M A De Gryse, Sebastiaan Theuns, Hans NauwynckAbstract:The replication cycle of white spot syndrome virus (WSSV) was investigated in secondary cell cultures from the Lymphoid Organ of Litopenaeus vannamei. The secondary cells formed a confluent monolayer at 24 h post-reseeding, and this monolayer could be maintained for 10 days with a viability of 90 %. Binding of WSSV to cells reached a maximum (73 ± 3 % of cells and 4.84 ± 0.2 virus particles per virus-binding cell) at 120 min at 4 °C. WSSV entered cells by endocytosis. The co-localization of WSSV and early endosomes was observed starting from 30 min post-inoculation (p.i.). Double indirect immunofluorescence staining showed that all cell-bound WSSV particles entered these cells in the period between 0 and 60 min p.i. and that the uncoating of WSSV occurred in the same period. After 1 h inoculation at 27 °C, the WSSV nucleocapsid protein VP664 and envelope protein VP28 started to be synthesized in the cytoplasm from 1 and 3 h p.i., and were transported into nuclei from 3 and 6 h p.i., respectively. The percentage of cells that were VP664- and VP28-positive in their nuclei peaked (50 ± 4 %) at 12 h p.i. Quantitative PCR showed that WSSV DNA started to be synthesized from 6 h p.i. In vivo titration of the supernatants showed that the progeny WSSV were released from 12 h p.i. and peaked at 18 h p.i. In conclusion, the secondary cell cultures from the Lymphoid Organ were proven to be ideal for examination of the replication cycle of WSSV.
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Eye extract improves cell migration out of Lymphoid Organ explants of L. vannamei and viability of the primary cell cultures
In Vitro Cellular & Developmental Biology - Animal, 2015Co-Authors: Vo Tuan, Khuong Thuong, Peter Bossier, Hans NauwynckAbstract:Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 ( w / v ) suspension) from different shrimp Organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary Lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the Lymphoid Organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the Lymphoid Organ of L. vannamei .
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characterization of a primary cell culture from Lymphoid Organ of litopenaeus vannamei and use for studies on wssv replication
Aquaculture, 2014Co-Authors: Van Thao Nguyen, Peter Bossier, Mathias Corteel, J J Dantaslima, Khuong Van Thuong, Vo Van Tuan, Patrick Sorgeloos, Hans NauwynckAbstract:Abstract Shrimp aquaculture is a booming agro-industry worldwide. Due to intensification of shrimp farming, pathogens emerge. Control of these pathogens especially viral pathogens is essential for a further expansion of this industry. Until now, the lack of shrimp cell lines has limited research on shrimp viral pathogens. In this context, a primary culture from the Lymphoid Organ of Litopenaeus vannamei was developed and standardized as a platform for further research on white spot syndrome virus (WSSV). Explants from the Lymphoid Organ of L. vannamei were cultured in 2 × L-15 (Leibovitz-15) medium supplemented with 20% fetal bovine serum (FBS), 10% Chen's salt, penicillin (100 IU/ml) & streptomycin (100 μg/ml), gentamicin (50 μg/ml) and fungizone (0.25 μg/ml) with a pH of 7.5. Gelatin (0.1%)-coated culture plates promoted the migration of cells from the explants and cell survival. 600 μg/ml cholesterol and 1000 μg/ml l -glutathione (GSH) both enhanced cell survival and performance in vitro . Susceptibility of Lymphoid Organ cells for infection with white spot syndrome virus (WSSV) was determined by indirect immunofluorescence (IIF) staining by monoclonal antibodies against VP28 (W29) and goat anti-mouse IgG-FITC. FITC positive signals in the nuclei starting from 9 h post inoculation (hpi) demonstrated the susceptibility of the cells for WSSV infection in these cultures. This cell culture system will be used in the future as a tool for studying host–virus interactions in WSSV pathogenesis.
