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Samson T Jacob - One of the best experts on this subject based on the ideXlab platform.
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peptide mass mapping of acetylated isoforms of histone h4 from mouse Lymphosarcoma cells treated with histone deacetylase hdacs inhibitors
Journal of the American Society for Mass Spectrometry, 2005Co-Authors: Chen Ren, Kalpana Ghoshal, Liwen Zhang, Michael A Freitas, Mark R Parthun, Samson T JacobAbstract:The acetylated isoforms of histone H4 from mouse Lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse Lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
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suppression of metallothionein i ii expression and its probable molecular mechanisms
Environmental Health Perspectives, 2002Co-Authors: Samson T Jacob, Sarmila Majumder, Kalpana GhoshalAbstract:Metallothionein (MT) promoter was methylated in rat hepatoma and in mouse Lymphosarcoma cells by methylation of cytosine within the CpG dinucleotide region. After demethylation of MT-I promoter in ...
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Silencing of metallothionein-I gene in mouse Lymphosarcoma cells by methylation
Oncogene, 1999Co-Authors: Sarmila Majumder, Kalpana Ghoshal, Zhiling Li, Yuan Bo, Samson T JacobAbstract:Metallothionein-I ( MT-I ) gene is silenced by methylation of CpG islands in mouse Lymphosarcoma P1798 cells but not in the thymus, the cell type from which the tumor was derived. Bisulfite genomic sequencing revealed that all 21 CpG dinucleotides present within −216 bp to +1 bp with respect to transcription start site are methylated in the tumor cell line, but none is methylated in the thymus. The Lymphosarcoma cells induced MT-I in response to heavy metals only after demethylation with 5-azacytidine (5-AsaC). The electrophoretic mobility shift assay using specific oligonucleotide probes showed that the key transcription factors regulating MT-I gene (e.g., MTF-1, Sp 1 and MLTF/USF) are active in P1798 cells. In vivo footprinting of the proximal promoter region showed that none of the metal regulatory elements (MREs) or MLTF/USF are occupied in response to heavy metals. Demethylation of the Lymphosarcoma cells with 5-AzaC resulted in constitutive footprinting at MLTF/ARE, and zinc-inducible footprinting at MRE-c, MRE-d and MRE-e sites. Demethylation of just 10 – 20% of the CpG islands was sufficient to render the gene inducible by cadmium or zinc. The MT-I induction persisted in the cancer cells for several generations even after withdrawal of 5-AzaC from the culture medium.
Gregory B. Daniel - One of the best experts on this subject based on the ideXlab platform.
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J Vet Diagn Invest 20:656–660 (2008) Diffuse intestinal T-cell Lymphosarcoma in a yellow-naped Amazon parrot (Amazona ochrocephala auropalliata)
2016Co-Authors: Marcy J. Souza, Shelley J. Newman, Cheryl B. Greenacre, James S. Avenell, Jonathan S. Wall, Jeffrey C. Phillips, Michael M. Fry, Robert L. Donnell, Gregory B. DanielAbstract:Abstract. A 10-year-old, intact, female yellow-naped Amazon parrot (Amazona ochrocephala auropalliata) was examined because of anemia, lymphocytic leukocytosis, regurgitation, and weight loss. A positive fecal occult blood and monoclonal globulinopathy were present. A distended proventriculus and diffusely thickened loops of small intestine with irregular luminal surfaces were identified with contrast radiography and contrast computed tomography. A micro positron emission tomography scan was performed with 18F-fluoro-deoxyglucose. Diffuse intestinal T-cell Lymphosarcoma was diagnosed based on histopathology and immunohistochemistry of full thickness small intestinal biopsies. The patient was treated with a multidrug chemotherapy protocol with little to no effect. Euthanasia was elected, and intestinal Lymphosarcoma was confirmed on histopathology of necropsy intestinal samples; no other organs demonstrated neoplastic infiltration. To the authors ’ knowledge, no reports are currently available detailing the clinical presentation or diagnosis of diffuse intestinal T-cell Lymphosarcoma in any avian species. Key words: Avian; immunohistochemistry; Lymphosarcoma; positron emission tomography. 1 Lymphosarcoma (LSA) is the most commonly reported lymphoid neoplasm in parrots and has been reported i
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diffuse intestinal t cell Lymphosarcoma in a yellow naped amazon parrot amazona ochrocephala auropalliata
Journal of Veterinary Diagnostic Investigation, 2008Co-Authors: Marcy J. Souza, Shelley J. Newman, Cheryl B. Greenacre, James S. Avenell, Jonathan S. Wall, Jeffrey C. Phillips, Michael M. Fry, Robert L. Donnell, Gregory B. DanielAbstract:A 10-year-old, intact, female yellow-naped Amazon parrot (Amazona ochrocephala auropalliata) was examined because of anemia, lymphocytic leukocytosis, regurgitation, and weight loss. A positive fecal occult blood and monoclonal globulinopathy were present. A distended proventriculus and diffusely thickened loops of small intestine with irregular luminal surfaces were identified with contrast radiography and contrast computed tomography. A micro positron emission tomography scan was performed with 18 F-fluoro- deoxyglucose. Diffuse intestinal T-cell Lymphosarcoma was diagnosed based on histopathology and immunohistochemistry of full thickness small intestinal biopsies. The patient was treated with a multidrug chemotherapy protocol with little to no effect. Euthanasia was elected, and intestinal Lymphosarcoma was confirmed on histopathology of necropsy intestinal samples; no other organs demonstrated neoplastic infiltration. To the authors' knowledge, no reports are currently available detailing the clinical presentation or diagnosis of diffuse intestinal T-cell Lymphosarcoma in any avian species.
Kalpana Ghoshal - One of the best experts on this subject based on the ideXlab platform.
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peptide mass mapping of acetylated isoforms of histone h4 from mouse Lymphosarcoma cells treated with histone deacetylase hdacs inhibitors
Journal of the American Society for Mass Spectrometry, 2005Co-Authors: Chen Ren, Kalpana Ghoshal, Liwen Zhang, Michael A Freitas, Mark R Parthun, Samson T JacobAbstract:The acetylated isoforms of histone H4 from mouse Lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse Lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
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suppression of metallothionein i ii expression and its probable molecular mechanisms
Environmental Health Perspectives, 2002Co-Authors: Samson T Jacob, Sarmila Majumder, Kalpana GhoshalAbstract:Metallothionein (MT) promoter was methylated in rat hepatoma and in mouse Lymphosarcoma cells by methylation of cytosine within the CpG dinucleotide region. After demethylation of MT-I promoter in ...
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Silencing of metallothionein-I gene in mouse Lymphosarcoma cells by methylation
Oncogene, 1999Co-Authors: Sarmila Majumder, Kalpana Ghoshal, Zhiling Li, Yuan Bo, Samson T JacobAbstract:Metallothionein-I ( MT-I ) gene is silenced by methylation of CpG islands in mouse Lymphosarcoma P1798 cells but not in the thymus, the cell type from which the tumor was derived. Bisulfite genomic sequencing revealed that all 21 CpG dinucleotides present within −216 bp to +1 bp with respect to transcription start site are methylated in the tumor cell line, but none is methylated in the thymus. The Lymphosarcoma cells induced MT-I in response to heavy metals only after demethylation with 5-azacytidine (5-AsaC). The electrophoretic mobility shift assay using specific oligonucleotide probes showed that the key transcription factors regulating MT-I gene (e.g., MTF-1, Sp 1 and MLTF/USF) are active in P1798 cells. In vivo footprinting of the proximal promoter region showed that none of the metal regulatory elements (MREs) or MLTF/USF are occupied in response to heavy metals. Demethylation of the Lymphosarcoma cells with 5-AzaC resulted in constitutive footprinting at MLTF/ARE, and zinc-inducible footprinting at MRE-c, MRE-d and MRE-e sites. Demethylation of just 10 – 20% of the CpG islands was sufficient to render the gene inducible by cadmium or zinc. The MT-I induction persisted in the cancer cells for several generations even after withdrawal of 5-AzaC from the culture medium.
Marcy J. Souza - One of the best experts on this subject based on the ideXlab platform.
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J Vet Diagn Invest 20:656–660 (2008) Diffuse intestinal T-cell Lymphosarcoma in a yellow-naped Amazon parrot (Amazona ochrocephala auropalliata)
2016Co-Authors: Marcy J. Souza, Shelley J. Newman, Cheryl B. Greenacre, James S. Avenell, Jonathan S. Wall, Jeffrey C. Phillips, Michael M. Fry, Robert L. Donnell, Gregory B. DanielAbstract:Abstract. A 10-year-old, intact, female yellow-naped Amazon parrot (Amazona ochrocephala auropalliata) was examined because of anemia, lymphocytic leukocytosis, regurgitation, and weight loss. A positive fecal occult blood and monoclonal globulinopathy were present. A distended proventriculus and diffusely thickened loops of small intestine with irregular luminal surfaces were identified with contrast radiography and contrast computed tomography. A micro positron emission tomography scan was performed with 18F-fluoro-deoxyglucose. Diffuse intestinal T-cell Lymphosarcoma was diagnosed based on histopathology and immunohistochemistry of full thickness small intestinal biopsies. The patient was treated with a multidrug chemotherapy protocol with little to no effect. Euthanasia was elected, and intestinal Lymphosarcoma was confirmed on histopathology of necropsy intestinal samples; no other organs demonstrated neoplastic infiltration. To the authors ’ knowledge, no reports are currently available detailing the clinical presentation or diagnosis of diffuse intestinal T-cell Lymphosarcoma in any avian species. Key words: Avian; immunohistochemistry; Lymphosarcoma; positron emission tomography. 1 Lymphosarcoma (LSA) is the most commonly reported lymphoid neoplasm in parrots and has been reported i
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diffuse intestinal t cell Lymphosarcoma in a yellow naped amazon parrot amazona ochrocephala auropalliata
Journal of Veterinary Diagnostic Investigation, 2008Co-Authors: Marcy J. Souza, Shelley J. Newman, Cheryl B. Greenacre, James S. Avenell, Jonathan S. Wall, Jeffrey C. Phillips, Michael M. Fry, Robert L. Donnell, Gregory B. DanielAbstract:A 10-year-old, intact, female yellow-naped Amazon parrot (Amazona ochrocephala auropalliata) was examined because of anemia, lymphocytic leukocytosis, regurgitation, and weight loss. A positive fecal occult blood and monoclonal globulinopathy were present. A distended proventriculus and diffusely thickened loops of small intestine with irregular luminal surfaces were identified with contrast radiography and contrast computed tomography. A micro positron emission tomography scan was performed with 18 F-fluoro- deoxyglucose. Diffuse intestinal T-cell Lymphosarcoma was diagnosed based on histopathology and immunohistochemistry of full thickness small intestinal biopsies. The patient was treated with a multidrug chemotherapy protocol with little to no effect. Euthanasia was elected, and intestinal Lymphosarcoma was confirmed on histopathology of necropsy intestinal samples; no other organs demonstrated neoplastic infiltration. To the authors' knowledge, no reports are currently available detailing the clinical presentation or diagnosis of diffuse intestinal T-cell Lymphosarcoma in any avian species.
P J Canfield - One of the best experts on this subject based on the ideXlab platform.
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immunohistochemical characterization of Lymphosarcoma in two alpacas lama pacos
Journal of Comparative Pathology, 2002Co-Authors: Susan Hemsley, G Bailey, P J CanfieldAbstract:Species cross-reactive anti-peptide antibodies were assessed in formalin-fixed tissue for use in immunophenotyping of Lymphosarcoma in two alpacas. Diagnosis of Lymphosarcoma was made by routine histopathological examination. Primary antibodies used for immunophenotyping were anti-human CD3 and anti-human CD5 for T cells; and anti-human CD79a and anti-human CD79b for B cells/plasma cells. In one case, most of the neoplastic cells were labelled with both anti-CD3 and anti-CD79b, and smaller numbers were labelled with anti-CD79a. The other case was classified as a B-cell tumour on the basis of labelling of the majority of neoplastic cells with anti-CD79b and anti-CD79a. This is the first recorded attempt at immunophenotyping Lymphosarcoma in alpacas and, to our knowledge, the first record of presumptive co-expression of T- and B-cell-associated molecules in Lymphosarcoma in the veterinary literature.
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feline immunodeficiency virus status of australian cats with Lymphosarcoma
Australian Veterinary Journal, 2001Co-Authors: L J Gabor, D N Love, R Malik, P J CanfieldAbstract:Objective To determine the FIV status of Australian cats with Lymphosarcoma and relate this to patient characteristics, tumour characteristics (tissue involvement, histological grade and immunophenotype), haematological and serum biochemical values and FeLV status of affected cats. Design Prospective study of 101 client-owned cats with naturally-occurring Lymphosarcoma. Procedure Western blot analysis, ELISA and immunochromatography were used to detect FIV antibodies in serum from cats with Lymphosarcoma. Results On the basis of Western blot analysis (which was considered the most accurate method for determining FIV status), 50/101 (50%) of cats with naturally-occurring Lymphosarcoma were positive for FIV antibodies. Of these 50 cats, 35 had tumours of B-cell phenotype, 13 had T-cell tumours and 2 had tumours classified as non-B/non-T. Tumours from eight of these FIV-positive cats contained FeLV gene sequences, including a 9-month-old cat with FeLV antigenaemia. Compared with FIV-negative cats with Lymphosarcoma, FIV-positive cats were more likely to be domestic crossbreds (P = 0.004), male (P = 0.048) and have atypical (especially nasal) forms of Lymphosarcoma (P = 0.09). Only 39 of 107 (36%) blood or sera tested using ELISA were positive for FIV antibodies (including 5 false-positives). Conclusions The prevalence of FIV infection was considerably higher in our cohort of cats compared with series of Lymphosarcoma cases from the Northern hemisphere. A positive FIV status was strongly associated with Lymphosarcoma in Australian cats and it is possible that this infection may predispose to the development of lymphoid neoplasia. The presence of FIV infection would have been underestimated if commercial kits alone had been used for serology.
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feline leukaemia virus status of australian cats with Lymphosarcoma
Australian Veterinary Journal, 2001Co-Authors: L J Gabor, R Malik, M L Jackson, B Trask, P J CanfieldAbstract:Objective To determine the FeLV status of sera and tumours from Australian cats with Lymphosarcoma in relation to patient characteristics, tumour characteristics (tissue involvement, histological grade and immunophenotype), haematological and biochemical values. Design Prospective study of 107 client-owned cats with naturally-occurring Lymphosarcoma. Procedure An ELISA was used to detect FeLV p27 antigen in serum specimens collected from cats with Lymphosarcoma. A PCR was used to detect FeLV DNA in formalin-fixed, paraffin-embedded tissue sections containing neoplastic lymphoid cells. The PCR was designed to amplify a highly conserved region of the untranslated long terminal repeat of FeLV provirus. Results Only 2 of 107 cats (2%), for which serum samples were available, were FeLV-positive on the basis of detectable p27 antigen in serum. In contrast, 25 of 97 tumours (26%) contained FeLV DNA. Of the 86 cats for which both PCR and ELISA data were available, 19(22%) had FeLV provirus in their tumours but no detectable circulating FeLV antigen in serum, while 2 (2%) had FeLV provirus and circulating FeLV antigen. FeLV PCR-positive/ELISA-negative cats (19) differed from PCR-negative/ELISA-negative cats (65) in having fewer B-cell tumours (P = 0.06), more non B-/non T-cell tumours (P = 0.02) and comprising fewer non-Siamese/Oriental pure-bred cats (P = 0.03). Conclusions The prevalence of FeLV antigen or provirus was considerably lower in our cohort of cats compared with studies of Lymphosarcoma conducted in the Northern hemisphere. This suggests that factors other than FeLV are important in the development of Lymphosarcoma in many Australian cats. No firm conclusions could be drawn concerning whether FeLV provirus contributed to the development of Lymphosarcoma in PCR-positive/ELISA-negative cats.
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immunophenotypic and histological characterisation of 109 cases of feline Lymphosarcoma
Australian Veterinary Journal, 1999Co-Authors: L J Gabor, P J Canfield, R MalikAbstract:Objective To determine and analyse the immunophenotype and histological appearance of naturally occurring cases of Lymphosarcoma in Australian cats. Design A prospective multi-institutional study of naturally occurring feline Lymphosarcoma. Methods One hundred and eighteen cats were referred for diagnosis and/or management of suspected Lymphosarcoma. Tissue samples for histopathological analysis and immunophenotyping were collected as biopsies or at necropsy from 109 cases. Histological classification of the neoplasms followed the Working Formulation Classification System. Four multi-species cross-reactive antibodies were used to classify tumours as having a B or T cell phenotype. Results Seventy-six (70%) cases were B cell tumours and 28 (26%) were T cell tumours. The remaining 5 (4%) specimens failed to stain with the four antibodies. Histologically, 11 (10%) cases were classified as low-grade, 72 (66%) were medium-grade and 26 (24%) were high-grade tumours. There were no significant associations between age and either histological grade or immunophenotype. Mediastinal and leukaemic cases were significantly more likely to be T cell tumours (P < 0.001 and P < 0.001, respectively). Conclusions In contrast to previously documented studies in the cat, the majority of cases of Lymphosarcoma were of B cell phenotype and intermediate histological grade. Based on our data, the histological phenotype of Lymphosarcoma is unlikely to predict immunotype, nor are cases of certain immunotypes likely to be of specific histological subtype. Considered in relation to previous reports, the findings suggest that epidemiological factors operating in these cats to produce Lymphosarcoma may be different to those operating in North American and UK cat populations.
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clinical and anatomical features of Lymphosarcoma in 118 cats
Australian Veterinary Journal, 1998Co-Authors: L J Gabor, R Malik, P J CanfieldAbstract:Objective To determine patients' characteristics and anatomical distribution of lesions in cats with Lymphosarcoma. Design Prospective multi-institutional study of naturally occurring feline Lymphosarcoma. Methods Veterinarians in Sydney were provided with free diagnostic laboratory services for suspect cases of feline lym-phosarcoma. Lymphosarcoma was diagnosed based on physical findings, radiographic and/or ultrasonographic images and results of cytological or histopathological examination. When owners were not interested in pursuing an antemortem diagnosis, suspect cases were collected for necropsy. Patients' characteristics and physical findings were recorded. A modified scheme for anatomical classification of lesions was devised including a ‘mixed’ category for cases which involved two or more anatomical forms. Results One hundred and eighteen cases were accrued over an 18 month period. The median age was 120 months and range 5 to 212 months. Age distribution was bimodal, with a small peak for cats less than 24 months, and a normal distribution centred on 97 to 120 months. Eighty cats were domestic crossbreds, 22 were Siamese or Oriental cats (including crosses), 6 were Burmese, 5 were purebred longhairs and the remaining 5 were one of a number of purebred shorthaired breeds. In comparison to 1017 consecutive cases admitted to our hospital for conditions other than Lymphosarcoma, Siamese/Oriental cats were over-represented amongst Lymphosarcoma cases (P = 0.0006). Male cats were also over-represented, accounting for 72 of 118 cases (P = 0.05). Abdominal Lymphosarcoma was the most common anatomical form (43 cats), followed by mixed (39), nodal (20), mediastinal (9) and atypical (involving non-lymphoid organs, 7) forms. When analysed for specific organ involvement, 29 (25%) had mediastinal involvement, 71 (60%) had abdominal involvement including 60 (51%) with involvement of the intestinal tract and/or mesenteric lymph nodes and 36 (31%) with bilateral renal involvement, and 47 (40%) had peripheral lymph node involvement. No case of primary lymphoid leukaemia was identified. A noticeable subgroup of cats younger than 24 months had involvement of the anterior mediastinum with or without concurrent enlargement of cervical or axillary lymph nodes; Siamese/Oriental cats were over-represented in this subgroup. Among cases with nodal involvement, lymph nodes of the head and neck were frequently involved, mandibular nodes most commonly, followed by superficial cervical nodes. In seven cases a solitary node was affected. Conclusions Compared with similar surveys overseas, our cats were older and male cats were over-represented. There was a notable subgroup of young cats with mediastinal involvement. Siamese/Oriental cats were over-represented in this subgroup as well as in the larger population of cats with Lymphosarcoma. Compared with overseas surveys, renal involvement, mixed cases and atypical cases (including nasal Lymphosarcoma) were more common. A new subcategory of nodal Lymphosarcoma, with involvement restricted to node(s) of head and neck, was identified.
