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Martin A. Erlandson - One of the best experts on this subject based on the ideXlab platform.
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Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): A new European species of alphabaculovirus with a narrow host range.
PLOS ONE, 2017Co-Authors: Oihane Simón, Martin A. Erlandson, Marie Frayssinet, Trevor Williams, David A. Theilmann, Anne-nathalie Volkoff, Primitivo CaballeroAbstract:During an insect sampling program in alfalfa crops near Montpellier, France in 2011, Lacanobia oleracea larvae were collected that died due to nucleopolyhedrovirus infection (LaolNPV). This virus was subjected to molecular and biological characterization. The virus was a multiple nucleocapsid NPV that showed similar restriction profiles to Mamestra configurata NPV-A (MacoNPV-A) but with significant differences. Polypeptide analysis demonstrated similar proteins in occlusion bodies and occlusion derived virions, to those observed in NPVs from Mamestra spp. Terminal sequencing revealed that the genome organization shared similarity with that of MacoNPV-A. The most homologous virus was MacoNPV-A 90/2 isolate (95.63% identity and 96.47% similarity), followed by MacoNPV-A 90/4 strain (95.37% and 96.26%), MacoNPV-B (89.21% and 93.53%) and M. brassicae MNPV (89.42% and 93.74%). Phylogenetic analysis performed with lef-8, lef-9, polh and a concatenated set of genes showed that LaolNPV and the Mamestra spp. NPVs clustered together with HaMNPV, but with a closer genetic distance to MacoNPV-A strains. The Kimura 2-parameter (K-2-P) distances of the complete genes were greater than 0.05 between LaolNPV and the MbMNPV/MacoNPV-B/HaMNPV complex, which indicates that LaolNPV is a distinct species. K-2-P distances were in the range 0.015-0.050 for comparisons of LaolNPV with MacoNPV-A strains, such that additional biological characteristics should be evaluated to determine species status. While MacoNPV-A was pathogenic to seven lepidopteran species tested, LaolNPV was only pathogenic to Chrysodeixis chalcites. Given these findings, Lacanobia oleracea nucleopolyhedrovirus should be considered as a new species in the Alphabaculovirus genus.
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Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae.
Insect Molecular Biology, 2010Co-Authors: Umut Toprak, Martin A. Erlandson, Cedric Gillott, Doug Baldwin, Dwayne D. HegedusAbstract:Insect intestinal mucins (McIIM2-4) expressed in the midgut of feeding, starved and moulting Mamestra configurata larvae were identified. McIIM2 and McIIM4 were associated with the peritrophic matrix (PM). PMs from feeding and starved larvae were translucent and contained organized chitin bundles perpendicular to their long axis, whereas PM from moulting larvae consisted of an inner opaque mass surrounded by an outer translucent sleeve. Serine protease genes (McSP1, McSP2, McSP25 and McSP29) were also expressed in these larvae and several serine proteases were associated with the PM. Serine protease activity was also detected in the midgut of feeding, starved and moulting larvae.
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A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix.
Insect Molecular Biology, 2008Co-Authors: Umut Toprak, Martin A. Erlandson, Cedric Gillott, Doug Baldwin, X. Hou, Cathy Coutu, Dwayne D. HegedusAbstract:One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.
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Effect of Flavonoids on Feeding Preference and Development of the Crucifer Pest Mamestra configurata Walker
Journal of Chemical Ecology, 2004Co-Authors: Joseph C. Onyilagha, Jennifer Lazorko, Margaret Y. Gruber, Juliana J. Soroka, Martin A. ErlandsonAbstract:Thirty-seven flavonoid compounds (9 flavones, 18 flavonols, 8 flavanones, and 2 flavanonols) were investigated for their effect on feeding choice with bertha armyworm ( Mamestra configurata Walker; BAW). Feeding choice was dependent upon subtle differences in biochemical structure. Unsubstituted flavone and flavanone were the strongest feeding deterrents in the choice bioassay, while 7,4′-dihydroxyflavone and dihydroquercetin stimulated BAW to feed. The constitutive flavonoids of Brassica napus , isorhamnetin-3-sophoroside-7-glucoside and kaempferol-3,7-diglucoside, were effective deterrents when supplemented at concentrations higher than endogenous levels. In a no-choice bioassay, flavone reduced both larval weight as well as larval and pupal development time.
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Potential impact of spinosad on parasitoids of Mamestra configurata (Lepidoptera: Noctuidae)
The Canadian Entomologist, 2002Co-Authors: Peter G. Mason, Martin A. Erlandson, R.h. Elliott, B.j. HarrisAbstract:On trouvera ici les resultats d'une etude sur les effets d'un insecticide, le spinosad, un derive biologique, sur les parasitoides de Mamestra configurata Walker, la Legionnaire bertha. Les adultes de Trichogramma inyoense Pinto et Oatman (Hymenoptera: Trichogrammatidae), un parasitoide des oeufs, se sont averes aussi sensibles a une dose de 0,125 μg/cm 2 qu'a une forte dose de 2,0 μg/cm 2 . L'application de spinosad aux oeufs de l'hote au moment de la ponte a eu pour resultat de reduire le parasitisme de T. inyoense et donne lieu a une mortalite accrue des parasitoides dans les oeufs parasites. Les adultes de Microplitis mediator (Haliday) (Hymenoptera: Braconidae), endoparasite larvaire, se sont montres tres sensibles a la plus haute dose testee, 1,0 μg/cm 2 . Du spinosad a raison de 2,0 μg/cm 2 , administre a des larves de legionnaires parasitees par M. mediator 1 a 7 jours auparavant a entraine une mortalite du meme ordre que celle enregistree dans le cas de larves non parasitees. A une dose de 0,125 μg/cm 2 , seules les larves parasitees 1 jour auparavant avaient des taux de mortalite semblables a ceux des larves non parasitees et cette faible dose est restee sans effet sur les larves parasitees 7 jours auparavant. Les larves de Microplitis mediator emergees d'hotes parasites 7 jours auparavant et traites a une faible dose de spinosad ont ete incapables de completer la construction de leur cocon et elles sont mortes. L'integration de spinosad dans un programme de lutte contre Mamestra configurata devrait comporter une evaluation prealable de la presence et de l'abondance de parasitoides.
Dwayne D. Hegedus - One of the best experts on this subject based on the ideXlab platform.
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Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae.
Insect Molecular Biology, 2010Co-Authors: Umut Toprak, Martin A. Erlandson, Cedric Gillott, Doug Baldwin, Dwayne D. HegedusAbstract:Insect intestinal mucins (McIIM2-4) expressed in the midgut of feeding, starved and moulting Mamestra configurata larvae were identified. McIIM2 and McIIM4 were associated with the peritrophic matrix (PM). PMs from feeding and starved larvae were translucent and contained organized chitin bundles perpendicular to their long axis, whereas PM from moulting larvae consisted of an inner opaque mass surrounded by an outer translucent sleeve. Serine protease genes (McSP1, McSP2, McSP25 and McSP29) were also expressed in these larvae and several serine proteases were associated with the PM. Serine protease activity was also detected in the midgut of feeding, starved and moulting larvae.
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A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix.
Insect Molecular Biology, 2008Co-Authors: Umut Toprak, Martin A. Erlandson, Cedric Gillott, Doug Baldwin, X. Hou, Cathy Coutu, Dwayne D. HegedusAbstract:One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.
Joseph C. Onyilagha - One of the best experts on this subject based on the ideXlab platform.
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Effect of Flavonoids on Feeding Preference and Development of the Crucifer Pest Mamestra configurata Walker
Journal of Chemical Ecology, 2004Co-Authors: Joseph C. Onyilagha, Jennifer Lazorko, Margaret Y. Gruber, Juliana J. Soroka, Martin A. ErlandsonAbstract:Thirty-seven flavonoid compounds (9 flavones, 18 flavonols, 8 flavanones, and 2 flavanonols) were investigated for their effect on feeding choice with bertha armyworm ( Mamestra configurata Walker; BAW). Feeding choice was dependent upon subtle differences in biochemical structure. Unsubstituted flavone and flavanone were the strongest feeding deterrents in the choice bioassay, while 7,4′-dihydroxyflavone and dihydroquercetin stimulated BAW to feed. The constitutive flavonoids of Brassica napus , isorhamnetin-3-sophoroside-7-glucoside and kaempferol-3,7-diglucoside, were effective deterrents when supplemented at concentrations higher than endogenous levels. In a no-choice bioassay, flavone reduced both larval weight as well as larval and pupal development time.
Chie Goto - One of the best experts on this subject based on the ideXlab platform.
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Mamestra brassicae Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived from Xestia c-nigrum granulovirus in Larvae of Mamestra brassicae and Helicoverpa armigera (Lepidoptera: Noctuidae) on Cabbage
Journal of economic entomology, 2010Co-Authors: Shigeyuki Mukawa, Chie GotoAbstract:ABSTRACT The insecticidal effect of Mamestra brassicae nucleopolyhedrovirus (MabrNPV) T5 against Mamestra brassicae (L.) and Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), important pests of various vegetables and ornamental crops in Japan as well as many other countries, and the enhancing activity of proteins derived from occlusion bodies of Xestia c-nigrum granulovirus (XecnGV) α-4, which was named GVPs, on the infectivity of MabrNPV were evaluated in a bioassay with second-instar larvae fed on virus-applied cabbage, Brassica oleracea L. var. capitata, plants. The lethal concentrations of MabrNPV achieving 95% mortality (LC95) were estimated to be 7.7 × 105 and 1.8 × 105 OBs per ml for M. brassicae and H. armigera, respectively, with MabrNPV-alone treatment. Because the mean areas of cabbage leaf consumed by one larva in 60 h were not significantly different between M. brassicae and H. armigera, we conclude that MabrNPV is more infectious to H. armigera than to M. brassicae. When larvae were fe...
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Effect of proteins derived from granulovirus on the infectivity of Mamestra brassicae nucleopolyhedrovirus in Helicoverpa armigera (Lepidoptera: Noctuidae)
Applied Entomology and Zoology, 2008Co-Authors: Shigeyuki Mukawa, Chie Goto, Yoshito SuzukiAbstract:The enhancing effect of virion-free proteins derived from granulovirus (GVPs) on the infectivity of Mamestra brassicae nucleopolyhedrovirus (MabrNPV) was examined using an alternative host, Helicoverpa armigera (Hubner). Second-instar larvae were inoculated by diet contamination bioassay. The LC50 values of larvae inoculated with MabrNPV alone were calculated to be 24,000 to 96,000 polyhedra/g diet, which was equivalent to the values of the original host, Mamestra brassicae (L.), in our previous report. The addition of GVPs (0.1 mg/g diet) reduced the LC50 values to 1/13.8–1/38.4. Most MabrNPV-infected larvae died at the third or fourth instar, and the increase of MabrNPV concentration caused larval death at younger instars in both the MabrNPV alone treatment and the MabrNPV plus GVPs treatment. The lethal time shortened in relation to viral concentration for both the MabrNPV alone treatment and the MabrNPV plus GVPs treatment. Our results reveal the potential for simultaneous control of M. brassicae and H. armigera by adding GVPs as an enhancer to MabrNPV.
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Enhancement of Nucleopolyhedrovirus Infectivity Against Mamestra brassicae (Lepidoptera: Noctuidae) by Proteins Derived from Granulovirus and a Fluorescent Brightener
Journal of economic entomology, 2007Co-Authors: Shigeyuki Mukawa, Chie GotoAbstract:The synergistic enhancement of nucleopolyhedrovirus (NPV) infection by granuloviruses (GVs) is well documented; and a GV granule protein, named viral enhancin, has been identified as an active contributor to this effect. We detected the presence of two proteins with molecular mass of 93 and 108 kDa in granules of a GV isolated from Xestia c-nigrum (L.) (XecnGV) as candidates for enhancin, and we confirmed that at least the 108-kDa protein enhances the infectivity of Mamestra brassicae nucleopolyhedrovirus (MabrNPV). We tested the effect of virion-free proteins obtained from XecnGV granules (GVPs) on MabrNPV infection, and we made a comparison with an enhancing chemical, the stilbene-derived fluorescent brightener Tinopal. Bioassay was performed employing the diet contamination method, by using second instars of Mamestra brassicae (L.) (Lepidoptera: Noctuidae). The enhancing effects of GVPs (0.1 mg/g diet) and Tinopal (1 mg/g diet) were estimated to be 70.7–81.5-fold and 26.9–33.7-fold, respectively, as calculated from the LC50 values of MabrNPV with or without the additives. The additives reduced the lethal time of MabrNPV-infected larvae and they caused death at a younger instar. These results suggest that GVPs can enhance MabrNPV infection as effectively as Tinopal.
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In vivo characterization of a group II nucleopolyhedrovirus isolated from Mamestra brassicae (Lepidoptera: Noctuidae) in Japan.
Journal of General Virology, 2006Co-Authors: Shigeyuki Mukawa, Chie GotoAbstract:A Japanese isolate of Mamestra brassicae nucleopolyhedrovirus (MabrNPV) was identified phylogenetically as a group II nucleopolyhedrovirus (NPV) that is related closely to other NPVs isolated from Mamestra spp. based on nucleotide sequence data of its polh, egt and lef-3 genes. The multiplication of MabrNPV in M. brassicae larvae was characterized following inoculation at various doses and in combination with the fluorescent brightener Tinopal by measuring temporal changes in the concentrations of its viral DNA using real-time quantitative PCR. The growth curves of budded-virus replication were analysed by fitting the data of viral DNA concentration in the host haemolymph to a modified Gompertz model. When fifth-instar larvae were inoculated with an LD95 equivalent dose of MabrNPV and Tinopal, the time lag between the onset of primary and secondary infection was estimated to be 25 h. Another 65 h was required to reach a plateau titre equivalent to a level of 109 virions ml−1 in the haemolymph. All larvae died during the sixth instar following this inoculation regime. In contrast, following inoculation with a 1000-fold higher dose of MabrNPV and Tinopal, the time lag between the onset of primary and secondary infection was only 20 h. Subsequently, the same plateau titre was reached after a further 20 h. Following this inoculation regime, most larvae died during the fifth instar. Quantification of viral DNA by real-time quantitative PCR and application of the Gompertz model are valuable for the characterization of baculovirus replication in vivo.
Stanislav Trdan - One of the best experts on this subject based on the ideXlab platform.
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Seasonal dynamics of the cabbage armyworm (Mamestra brassicae (L.)) and the bright-line brown-eyes moth ( Mamestra oleracea (L.)) in Slovenia
Horticultural Science, 2018Co-Authors: Marko Devetak, Tanja Bohinc, M. Kač, Stanislav TrdanAbstract:Devetak M., Bohinc T., Kac M., Trdan S., 2014. Seasonal dynamics of the cabbage armyworm ( Mamestra brassicae [L.]) and the bright-line brown-eyes moth ( Mamestra oleracea [L.]) in Slovenia . Hort. Sci. (Prague), 41: 80–88. The cabbage armyworm ( Mamestra brassicae L.) and the bright-line brown-eyes moth ( Mamestra oleracea L.) are polyphagous insect species. From 2008 to 2010, we monitored the seasonal dynamics of both pests in two locations in Slovenia, Ljubljana and the Nova Gorica region. Pheromone traps (VARL + type) were used to precisely determine the occurrence of adults, i.e., the beginning and end of generations and the peaks of the populations. This allowed us to examine the relationship between the quantity of pests, average daily air temperature and average daily precipitation. Our results established that there were two generations of Mamestra brassicae per year in both locations; however, the seasonal dynamics of Mamestra oleracea was not as clear due to low trap catch. During 2008–2010 in Ljubljana, the average temperature during the peaks of the first generation of M. brassicae ranged from 16–19°C (sum of effective temperatures (SET) from 250°C to 375°C) and 20°C (SET from 986°C to 1,290°C) during the peaks of the second generation. We found no correlation between the average number of cabbage armyworm adults during the peaks of both generations and the mean air temperature 35 and 70 days prior to the peaks.
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Seasonal dynamics of the cabbage armyworm (Mamestra brassicae [L.]) and the bright-line brown-eyes moth (Mamestra oleracea [L.]) in Slovenia
Horticultural Science, 2014Co-Authors: Marko Devetak, Tanja Bohinc, M. Kač, Stanislav TrdanAbstract:The cabbage armyworm (Mamestra brassicae L.) and the bright-line brown-eyes moth (Mamestra oleracea L.) are polyphagous insect species. From 2008 to 2010, we monitored the seasonal dynamics of both pests in two locations in Slovenia, Ljubljana and the Nova Gorica region. Pheromone traps (VARL + type) were used to precisely determine the occurrence of adults, i.e., the beginning and end of generations and the peaks of the populations. This allowed us to examine the relationship between the quantity of pests, average daily air temperature and average daily precipitation. Our results established that there were two generations of Mamestra brassicae per year in both locations; however, the seasonal dynamics of Mamestra oleracea was not as clear due to low trap catch. During 2008–2010 in Ljubljana, the average temperature during the peaks of the first generation of M. brassicae ranged from 16–19°C (sum of effective temperatures (SET) from 250°C to 375°C) and 20°C (SET from 986°C to 1,290°C) during the peaks of the second generation. We found no correlation between the average number of cabbage armyworm adults during the peaks of both generations and the mean air temperature 35 and 70 days prior to the peaks.
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Cabbage moth (Mamestra brassicae [L.]) and bright-line brown-eyes moth (Mamestra oleracea [L.]) - presentation of the species, their monitoring and control measures.
Acta agriculturae Slovenica, 2010Co-Authors: Marko Devetak, Matej Vidrih, Stanislav TrdanAbstract:The paper describes polyphagous pests, the cabbage moth (Mamestra brassicae) and bright-line brown-eyes moth (Mamestra oleracea), which were not systematically investigated up to now in Slovenia. The cabbage moth, which is more abundant, preferably attacks Brassica plants, and its caterpillars are especially harmful in the cabbage. The paper deals with the morphology, distribution and methods of monitoring the pest populations and discuss on their control. The protection of vegetables from both pests is primarily based on the use of chemical insecticides. However, the use of natural enemies and various agro-technical measures can also be very important in diminishing the populations of the pests. With interlacing of all of these approaches, healthy and quality food can be produced even in the growing seasons with high attack of the pests mentioned.
