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Patrick De Clercq - One of the best experts on this subject based on the ideXlab platform.
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prey range of the predatory ladybird cryptolaemus montrouzieri
Biocontrol, 2014Co-Authors: Sara Maes, Jeanclaude Gregoire, Patrick De ClercqAbstract:The prey range of Cryptolaemus montrouzieri was studied in the laboratory to investigate whether the Mealybug destroyer can contribute to the suppression of other pest insects besides Mealybugs and to assess its potential impact on non-Mealybug populations as part of an environmental risk assessment for its use in biological control. Prey tested in these experiments were: tobacco aphid Myzus persicae nicotianae (Sulzer)(Hemiptera: Aphididae), pea aphid Acyrthosiphon pisum (Harris)(Hemiptera: Aphididae), tobacco whitefly Bemisia tabaci (Gennadius)(Hemiptera: Aleyrodidae), southern green stinkbug Nezara viridula (L.)(Hemiptera: Pentatomidae) eggs, western flower thrips Frankliniellaoccidentalis (Pergande)(Thysanoptera: Thripidae), two-spotted ladybird Adalia bipunctata (L.)(Coleoptera: Coccinellidae) eggs and eggs of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae). Larval survival was high to moderate when C. montrouzieri was provided with hemipteran prey and poor to zero when the ladybirds were provided with non-hemipteran prey. Females reared on M. persicae and A. pisum produced similar numbers of eggs as their counterparts fed the citrus Mealybug Planococcus citri (Risso)(Hemiptera: Pseudococcidae), but fecundity was significantly lower when the ladybirds were reared on B. tabaci nymphs or on A. bipunctata eggs. Prey species that were found to be less suitable for immature development of C. montrouzieri could still be an adequate food source for reproduction and survival of adult ladybirds. For example, only 8 % of the predator larvae reached the adult stage when provided with A. bipunctata eggs, but females that had developed on eggs of the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) and that were supplied with A. bipunctata eggs from adult emergence on, were only 35 % less fecund than females provided with Mealybugs in their adult life. The results are discussed in relation to the development of a suitable methodology for prey/host range testing in the framework of an environmental risk assessment for arthropod natural enemies.
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri
PloS one, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De Clercq, Hong PangAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
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effects of transgenic cry1ac cpti cotton on non target Mealybug pest ferrisia virgata and its predator cryptolaemus montrouzieri
PLOS ONE, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De ClercqAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
Yuhong Zhang - One of the best experts on this subject based on the ideXlab platform.
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri
PloS one, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De Clercq, Hong PangAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
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effects of transgenic cry1ac cpti cotton on non target Mealybug pest ferrisia virgata and its predator cryptolaemus montrouzieri
PLOS ONE, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De ClercqAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
Congshuang Deng - One of the best experts on this subject based on the ideXlab platform.
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non- Target Mealybug Pest Ferrisia virgata and Its Predator
2016Co-Authors: Cryptolaemus Montrouzieri, Ping Liu, Jiaqin Xie, Congshuang DengAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator whe
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri
PloS one, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De Clercq, Hong PangAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
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effects of transgenic cry1ac cpti cotton on non target Mealybug pest ferrisia virgata and its predator cryptolaemus montrouzieri
PLOS ONE, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De ClercqAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
Aunu Rauf - One of the best experts on this subject based on the ideXlab platform.
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influence of host stage on oviposition development and sex ratio of anagyrus lopezi de santis hymenoptera encyrtidae a parasitoid of the cassava Mealybug phenacoccus manihoti matile ferrero 1 hemiptera pseudococcidae
Jurnal Hama dan Penyakit Tumbuhan Tropika, 2020Co-Authors: Evie Adriani, Aunu Rauf, Pudjianto PudjiantoAbstract:Influence of host stage on oviposition, development, and sex ratio of Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae),a parasitoid of the cassava Mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). The parasitoidAnagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced from Thailand into Indonesia in early 2014 to controlthe invasive cassava Mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). Because of the need toproduce large numbers of high-quality females, research was conducted in the laboratory to determine host stage preferencefor A. lopezi on different instars of P. manihoti. Individual female wasps were exposed to first, second, third instar nymphs,and pre-reproductive adult Mealybugs. In the no-choice test, the frequency of parasitized hosts and the number of eggs laidper host was significantly higher in second and third instar nymphs as well as adult Mealybugs compared to first instarnymphs. In the two-choice test, third instars nymphs and adult Mealybugs were the most preferred host for oviposition.Immature development of parasitoids was faster and the ratio of female to male parasitoids was higher following ovipositionin second and third instar nymphs and pre-reproductive adult hosts, compared to the first instar nymphs. Our findingsindicate that the use of pre-reproductive adults as hosts in a mass-rearing program would be the most productive and fastestway to produce A. lopezi populations with a female-biased sex ratio. Field release of parasitoids should be conducted whenthe host’s third instar nymph is the most abundant because the period during which preferred and suitable host stages areavailable would be the longest.
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kutu putih singkong phenacoccus manihoti matile ferrero hemiptera pseudococcidae persebaran geografi di pulau jawa dan rintisan pengendalian hayati
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA, 2017Co-Authors: Budi Abduchalek, Aunu RaufAbstract:Cassava Mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae): Geographic distribution in Java and initiation of biological control. Cassava Mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Psedococcidae), is a recently introduced pest in Indonesia. Parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was imported to control the pest. Studies were conducted to determine geographic distribution of P. manihoti throughout Java, and to evaluate the potential of the parasitoid as a biological control agent. Geographic distribution of the pest was determined through field survey, whereas evaluation of the parasitoid was studied through cage experiment and field release. Our survey revealed that the cassava Mealybug has spread throughout Java. Heavy infestations caused shortened and distorted stems, complete defoliation, and stunted growth. In cages containing only Mealybugs, all cassava plants (100%) died after two months. Whereas in cages containing both Mealybugs and three pairs of parasitoid, rate of parasitization was 25% and plant mortality 20%. Parasitoids released in the field were able to survive, reproduce, and establish under Bogor climatic condition. These might indicate that parasitoid A. lopezi is a potential natural enemy to be used in biological control program of the cassava Mealybug.
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Laju enkapsulasi parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) oleh kutu putih singkong Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae)
Perhimpunan Entomologi Indonesia, 2017Co-Authors: Evie Adriani, Aunu Rauf, Pudjianto PudjiantoAbstract:Parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced into Indonesia in early 2014 to control the cassava Mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). The objective of study was to determine encapsulation rate of parasitoid A. lopezi by various host instars of P. manihoti. Observation of encapsulation rate was made by exposing a single mated female of parasitoid on Mealybug nymph-1, -2, -3, and adult in a plastic cage for 24 h. Mealybugs then were dissected and number of parasitoid eggs laid and those encapsulated were counted. Study revealed that rate of aggregate encapsulation was highest (8.4%) by adults, followed by nymph-3 (5.8%), nymph-2 (3.1%), and nymph-1 (1.1%). Rate of effective encapsulation by adults was 2.0%, whereas by nymphs about 1.0%. The low rate of encapsulation is believed not to reduce the effectiveness of parasitoid A. lopezi in the biological control of cassava Mealybug P. manihoti in Indonesia.
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Pengujian Kekhususan Inang Parasitoid Anagyrus Lopezi (De Santis) (Hymenoptera: Encyrtidae) Pada Empat Spesies Kutu Putih Yang Berasosiasi Dengan Tanaman Singkong
Jurnal Entomologi Indonesia, 2016Co-Authors: Rani Dessy Karyani, Nina Maryana, Aunu RaufAbstract:A parasitoid, Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced from Thailand into Indonesia in early 2014 to control the cassava Mealybug Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). To determine its potential uses and effect on non-target species, behavioural observation of the parasitoids were made on four species of Mealybugs, i.e. P. manihoti , Paracoccus marginatus Williams-Granara de Willink, Pseudococcus jackbeardsleyi Gimpel-Miller, and Ferrisia virgata Cockerell (Hemiptera: Pseudococcidae). For that purposes, a set of tests were conducted which includes host susceptability, preference, and suitability. Tests were conducted by exposing a female parasitoid to 3rd instar nymphs of each Mealybug species in a petri dish. For susceptability test, parasitoid A. lopezi encounterend P. manihoti more often (13.70 ± 7.18 visits per 30 minutes) as compared to P. marginatus (985 ± 10.24), P. jackbeardsleyi (6.60 ± 3.62), and F. virgata (5.75 ± 4.09). So did ovipositor probing occurred more on P. manihoti (8.20 ± 5.68 probes per 30 minutes) than on P. marginatus (0.70 ± 1.84), P. jackbeardsleyi (0.35 ± 0.68), and F. virgata (0.10 ± 0.45). For preference test, host encounter and ovipositor probing by the parasitoid were more common on P. manihoti as opposed to other Mealybug species. Out of four Mealybug species tested, P. manihoti was the only suitable host for parasitoid development, with the number of progenies emerged 7.40 ± 2.17 individuals per 3 female parasitoids exposed in 24 hour. Host specifity exhibited by parasitoid A. lopezi may prevent adverse effect to other Mealybug species inhabiting cassava fields.
Jiaqin Xie - One of the best experts on this subject based on the ideXlab platform.
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non- Target Mealybug Pest Ferrisia virgata and Its Predator
2016Co-Authors: Cryptolaemus Montrouzieri, Ping Liu, Jiaqin Xie, Congshuang DengAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator whe
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Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri
PloS one, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De Clercq, Hong PangAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.
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effects of transgenic cry1ac cpti cotton on non target Mealybug pest ferrisia virgata and its predator cryptolaemus montrouzieri
PLOS ONE, 2014Co-Authors: Yuhong Zhang, Ping Liu, Jiaqin Xie, Congshuang Deng, Patrick De ClercqAbstract:Recently, several invasive Mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of Mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of Mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target Mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the Mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, Mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the Mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the Mealybug F. virgata.