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Thibaut Malausa - One of the best experts on this subject based on the ideXlab platform.
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Integrative taxonomy methods reveal high mealybug (Hemiptera: Pseudococcidae) diversity in southern Brazilian fruit crops
Scientific Reports, 2017Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Mehmet Bora Kaydan, Ferran Palero, Thibaut Malausa, Marcos BottonAbstract:The Serra Gaúcha region is the most important temperate fruit-producing area in southern Brazil. Despite mealybugs (Hemiptera: Pseudococcidae) infesting several host plants in the region, there is a lack of information about the composition of species damaging different crops. A survey of mealybug species associated with commercial fruit crops (apple, persimmon, strawberry and grapes) was performed in Serra Gaúcha between 2013 and 2015, using both morphology and DNA analyses for species identification. The most abundant species were Pseudococcus viburni (Signoret), found on all four host plant species, and Dysmicoccus brevipes (Cockerell), infesting persimmon, vines and weeds. The highest diversity of mealybug species was found on persimmon trees, hosting 20 different taxa, of which Anisococcus granarae Pacheco da Silva & Kaydan, D . brevipes , Pseudococcus sociabilis Hambleton and Ps . viburni were the most abundant. A total of nine species were recorded in vineyards. Planococcus ficus (Signoret) and Pseudococcus longispinus (Targioni Tozzetti) were observed causing damage to grapes for the first time. A single species, Ps . viburni , was found associated with apples, while both Ps . viburni and Ferrisia meridionalis Williams were found on strawberry. Four of the mealybug species found represent new records for Brazil.
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Pre‐release host range determination of the parasitoid Allotropa burrelli for the biocontrol of Pseudococcus comstocki in France
Journal of Applied Entomology, 2017Co-Authors: Bastien Quaglietti, Alexandre Fleisch, Philippe Kreiter, P Gautier, Geraldine Groussier, Thibaut MalausaAbstract:We performed “no-choice” tests to study the host range of the parasitoid Allotropa burrelli (Muesebeck) (Hymenoptera: Platygastridae) for use against the Comstock mealybug, Pseudococcus comstocki (Kuwana) (Hemiptera: Pseudococcidae), in Southern France. We tested three Pseudococcidae species as potential non-target hosts: two species from the same genus (Pseudococcus longispinus and Pseudococcus viburni) and Planococcus citri. Allotropa burrelli did not parasitize any of the non-target mealybug species tested. No attempt of oviposition was recorded for the three species tested during the first 20 min of parasitoid release and no parasitism occurred in 6–8 hr of exposure of the mealybugs to the parasitoid.
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Pre-release host range determination of the parasitoid allotropa burrelli for the biocontrol of Pseudococcus comstocki in France
Journal of Applied Entomology, 2017Co-Authors: Bastien Quaglietti, Alexandre Fleisch, Philippe Kreiter, P Gautier, Geraldine Groussier, Nicolas Ris, Thibaut MalausaAbstract:We performed no-choice tests to study the host range of the parasitoid Allotropa burrelli (Muesebeck) (Hymenoptera: Platygastridae) for use against the Comstock mealybug, Pseudococcus comstocki (Kuwana) (Hemiptera: Pseudococcidae), in Southern France. We tested three Pseudococcidae species as potential non-target hosts: two species from the same genus (Pseudococcus longispinus and Pseudococcus viburni) and Planococcus citri. Allotropa burrelli did not parasitize any of the non-target mealybug species tested. No attempt of oviposition was recorded for the three species tested during the first 20min of parasitoid release and no parasitism occurred in 6-8hr of exposure of the mealybugs to the parasitoid.
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mealybug species from chilean agricultural landscapes and main factors influencing the genetic structure of Pseudococcus viburni
Scientific Reports, 2015Co-Authors: Margarita C. G. Correa, Eric Lombaert, Didier Crochard, Andres Alvear, Tania Zaviezo, Thibaut Malausa, Ferran PaleroAbstract:The present study aimed to characterize the distribution of mealybug species along Chilean agro-ecosystems and to determine the relative impact of host plant, management strategy, geography and micro-environment on shaping the distribution and genetic structure of the obscure mealybug Pseudococcus viburni. An extensive survey was completed using DNA barcoding methods to identify Chilean mealybugs to the species level. Moreover, a fine-scale study of Ps. viburni genetic diversity and population structure was carried out, genotyping 529 Ps. viburni individuals with 21 microsatellite markers. Samples from 16 localities were analyzed using Bayesian and spatially-explicit methods and the genetic dataset was confronted to host-plant, management and environmental data. Chilean crops were found to be infested by Ps. viburni, Pseudococcus meridionalis, Pseudococcus longispinus and Planococcus citri, with Ps. viburni and Ps. meridionalis showing contrasting distribution and host-plant preference patterns. Ps. viburni samples presented low genetic diversity levels but high genetic differentiation. While no significant genetic variance could be assigned to host-plant or management strategy, climate and geography were found to correlate significantly with genetic differentiation levels. The genetic characterization of Ps. viburni within Chile will contribute to future studies tracing back the origin and improving the management of this worldwide invader.
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Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in brazilian vineyards
PLoS ONE, 2014Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Marcos Botton, Aline Bertin, Aurelie Blin, Daniel Bernardi, Guylène Rignol, Thibaut MalausaAbstract:Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell), Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley), Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso), Pseudococcus viburni (Signoret), Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn) and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.
Marcos Botton - One of the best experts on this subject based on the ideXlab platform.
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Integrative taxonomy methods reveal high mealybug (Hemiptera: Pseudococcidae) diversity in southern Brazilian fruit crops
Scientific Reports, 2017Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Mehmet Bora Kaydan, Ferran Palero, Thibaut Malausa, Marcos BottonAbstract:The Serra Gaúcha region is the most important temperate fruit-producing area in southern Brazil. Despite mealybugs (Hemiptera: Pseudococcidae) infesting several host plants in the region, there is a lack of information about the composition of species damaging different crops. A survey of mealybug species associated with commercial fruit crops (apple, persimmon, strawberry and grapes) was performed in Serra Gaúcha between 2013 and 2015, using both morphology and DNA analyses for species identification. The most abundant species were Pseudococcus viburni (Signoret), found on all four host plant species, and Dysmicoccus brevipes (Cockerell), infesting persimmon, vines and weeds. The highest diversity of mealybug species was found on persimmon trees, hosting 20 different taxa, of which Anisococcus granarae Pacheco da Silva & Kaydan, D . brevipes , Pseudococcus sociabilis Hambleton and Ps . viburni were the most abundant. A total of nine species were recorded in vineyards. Planococcus ficus (Signoret) and Pseudococcus longispinus (Targioni Tozzetti) were observed causing damage to grapes for the first time. A single species, Ps . viburni , was found associated with apples, while both Ps . viburni and Ferrisia meridionalis Williams were found on strawberry. Four of the mealybug species found represent new records for Brazil.
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Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in brazilian vineyards
PLoS ONE, 2014Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Marcos Botton, Aline Bertin, Aurelie Blin, Daniel Bernardi, Guylène Rignol, Thibaut MalausaAbstract:Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell), Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley), Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso), Pseudococcus viburni (Signoret), Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn) and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.
Vitor C. Pacheco Da Silva - One of the best experts on this subject based on the ideXlab platform.
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Integrative taxonomy methods reveal high mealybug (Hemiptera: Pseudococcidae) diversity in southern Brazilian fruit crops
Scientific Reports, 2017Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Mehmet Bora Kaydan, Ferran Palero, Thibaut Malausa, Marcos BottonAbstract:The Serra Gaúcha region is the most important temperate fruit-producing area in southern Brazil. Despite mealybugs (Hemiptera: Pseudococcidae) infesting several host plants in the region, there is a lack of information about the composition of species damaging different crops. A survey of mealybug species associated with commercial fruit crops (apple, persimmon, strawberry and grapes) was performed in Serra Gaúcha between 2013 and 2015, using both morphology and DNA analyses for species identification. The most abundant species were Pseudococcus viburni (Signoret), found on all four host plant species, and Dysmicoccus brevipes (Cockerell), infesting persimmon, vines and weeds. The highest diversity of mealybug species was found on persimmon trees, hosting 20 different taxa, of which Anisococcus granarae Pacheco da Silva & Kaydan, D . brevipes , Pseudococcus sociabilis Hambleton and Ps . viburni were the most abundant. A total of nine species were recorded in vineyards. Planococcus ficus (Signoret) and Pseudococcus longispinus (Targioni Tozzetti) were observed causing damage to grapes for the first time. A single species, Ps . viburni , was found associated with apples, while both Ps . viburni and Ferrisia meridionalis Williams were found on strawberry. Four of the mealybug species found represent new records for Brazil.
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Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in brazilian vineyards
PLoS ONE, 2014Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Marcos Botton, Aline Bertin, Aurelie Blin, Daniel Bernardi, Guylène Rignol, Thibaut MalausaAbstract:Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell), Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley), Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso), Pseudococcus viburni (Signoret), Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn) and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.
Tania Zaviezo - One of the best experts on this subject based on the ideXlab platform.
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Synthesis of citrophilus mealybug sex pheromone using chrysanthemol extracted from Pyrethrum (Tanacetum cinerariifolium)
2018Co-Authors: Jan Bergmann, Tania Zaviezo, Jaime Tapia, Manuel Bravo, Fernanda M. FloresAbstract:A commercial pyrethrum extract was used as a source of chrysanthemol for the synthesis of the citrophilus mealybug (Pseudococcus calceolariae) sex pheromone. The chrysanthemic acid esters (pyrethrins I) were isolated and subsequently reduced to obtain chrysanthemol, which was used for ester pheromone synthesis. Field tests showed that the pheromone synthesized using plant-derived chrysanthemol was as attractive to male P. calceolariae as the pheromone obtained using a commercial isomeric chrysanthemol mixture.
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mealybug species from chilean agricultural landscapes and main factors influencing the genetic structure of Pseudococcus viburni
Scientific Reports, 2015Co-Authors: Margarita C. G. Correa, Eric Lombaert, Didier Crochard, Andres Alvear, Tania Zaviezo, Thibaut Malausa, Ferran PaleroAbstract:The present study aimed to characterize the distribution of mealybug species along Chilean agro-ecosystems and to determine the relative impact of host plant, management strategy, geography and micro-environment on shaping the distribution and genetic structure of the obscure mealybug Pseudococcus viburni. An extensive survey was completed using DNA barcoding methods to identify Chilean mealybugs to the species level. Moreover, a fine-scale study of Ps. viburni genetic diversity and population structure was carried out, genotyping 529 Ps. viburni individuals with 21 microsatellite markers. Samples from 16 localities were analyzed using Bayesian and spatially-explicit methods and the genetic dataset was confronted to host-plant, management and environmental data. Chilean crops were found to be infested by Ps. viburni, Pseudococcus meridionalis, Pseudococcus longispinus and Planococcus citri, with Ps. viburni and Ps. meridionalis showing contrasting distribution and host-plant preference patterns. Ps. viburni samples presented low genetic diversity levels but high genetic differentiation. While no significant genetic variance could be assigned to host-plant or management strategy, climate and geography were found to correlate significantly with genetic differentiation levels. The genetic characterization of Ps. viburni within Chile will contribute to future studies tracing back the origin and improving the management of this worldwide invader.
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Characterization of microsatellite DNA libraries from three mealybug species and development of microsatellite markers for Pseudococcus viburni (Hemiptera: Pseudococcidae).
Bulletin of Entomological Research, 2014Co-Authors: M C G Correa, Tania Zaviezo, Jean Le Maguet, Etienne Herrbach, Thibaut MalausaAbstract:Mealybugs (Hemiptera: Pseudococcidae) are important pests for crops worldwide. Different species, cryptic taxa under the same species name or even populations within a species can differ in biological characteristics, such as phenology, resistance to insecticides, virus transmission and susceptibility to natural enemies. Therefore, their management efficacy depends on their accurate identification. Microsatellite genetic markers are efficient in revealing the fine-scale taxonomic status of insects, both at inter- and intra-specific level. Despite their potential uses, microsatellites have been developed only for one mealybug species so far. Hence, it is unclear whether microsatellites may be useful to assess mealybug population differentiation and structuring. In this work, we tested the feasibility of developing microsatellite markers in mealybugs by: (i) producing and characterizing microsatellite DNA libraries for three species: Pseudococcus viburni, Pseudococcus comstocki and Heliococcus bohemicus, and (ii) by developing and testing markers for Ps. viburni. The obtained libraries contained balanced percentages of dinucleotide (ranging from 15 to 25%) and trinucleotide (from 5 to 17%) motifs. The marker setup for Ps. viburni was successful, although 70% of the primers initially tested were discarded for a lack of polymorphism. Finally, 25 markers were combined in two multiplex polymerase chain reactions with 21 displaying no evidence of deviation from Hardy-Weinberg equilibrium. Ps. viburni markers were tested on one population from France and one from Chile. The markers revealed a significant genetic differentiation between the two populations with an Fst estimate of 0.266.
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Molecular and morphological characterization of mealybugs (Hemiptera: Pseudococcidae) from Chilean vineyards.
Bulletin of Entomological Research, 2012Co-Authors: Margarita C. G. Correa, Jean-françois Germain, Thibaut Malausa, Tania ZaviezoAbstract:Mealybugs are major pests of grapevines worldwide. They cause economic losses by lowering the cosmetic value of fruits, reducing yields, transmitting viruses and resulting in the quarantine or rejection of produce in international trade. Knowledge of the species present in a vineyard is important for the adjustment of management strategies. We surveyed and accurately characterized the mealybugs infesting vineyards in one of the main production areas of Chile; 164 mealybugs were sampled from 26 vineyards in four regions of Chile and identified by DNA sequencing for two markers (cytochrome oxidase I and internal transcribed spacer 2) and morphological examination. Pseudococcus viburni (Signoret) was the most common species, followed by Pseudococcus meridionalis Prado and Pseudococcus cribata Gonzalez. Molecular variability at the COI and ITS2 loci was observed in both P. viburni and P. cribata. A comparison of haplotypes of P. viburni worldwide provides support for a recent hypothesis that this species is native to South America, a finding with direct consequences for management. Neither Pseudococcus longispinus (Targioni & Tozzetti) nor Planococcus ficus Signoret were found.
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Development of a Multiplex PCR for Identification of Vineyard Mealybugs
Environmental Entomology, 2011Co-Authors: Kent M Daane, Vaughn M. Walton, Mathew C. Middleton, Monica L. Cooper, Douglas B. Walsh, René Sforza, Tania Zaviezo, Rodrigo P. P. AlmeidaAbstract:ABSTRACT A simple molecular tool was developed and tested to identify seven mealybug species found in North American vineyards: Pseudococcus maritimus Ehrhorn, Pseudococcus viburni (Signoret), Pseudococcus longispinus (Targioni-Tozzeti), Pseudococcus calceolariae (Maskell), Planococcus ficus (Signoret), Planococcus citri (Risso), and Ferrisia gilli Gullan. The developed multiplex PCR is based on the mitochondrial cytochrome c oxidase subunit one gene. In tests, this single-step multiplex PCR correctly identified 95 of 95 mealybug samples, representing all seven species and collected from diverse geographic regions. To test the sensitivity, single specimen samples with different Pl. ficus developmental stages (egg to adult female and adult male) were processed PCR and the resulting output provided consistent positive identification. To test the utility of this protocol for adult males caught in sex baited pheromone traps, Pl. ficus adult males were placed in pheromone traps, aged at a constant temperature ...
Mehmet Bora Kaydan - One of the best experts on this subject based on the ideXlab platform.
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mealybug species hemiptera coccomorpha pseudococcidae on soursop and sugar apple annonaceae in north east brazil with description of a new species of Pseudococcus westwood
Zootaxa, 2019Co-Authors: Vitor Pacheco C Da Silva, Mehmet Bora Kaydan, Christian S A Silvatorres, Jorge B TorresAbstract:Several species of Annonaceae are economically important fruit-tree crops in North-East Brazil, including Pernambuco state. However, in several regions within the state, the fruits are commonly infested by mealybugs (Hemiptera: Coccomorpha: Pseudococcidae). There is a lack of information about the mealybug species damaging this produce, so a survey of mealybug species associated with commercial sugar apple ( Annona squamosa L.) and soursop ( A. muricata L.) was conducted in the main production areas. The species Ferrisia virgata (Cockerell), Maconellicoccus hirsutus (Green), Planococcus minor (Maskell), Phenacoccus solenopsis Tinsley and Pseudococcus jackbeardsleyi Gimpel & Miller were found on both Annona species. Dysmicoccus brevipes (Cockerell), Ferrisia dayslirii Kaydan & Gullan and Ferrisia malvastra (MacDaniel) were found only on soursop; and Ferrisia cristinae Kaydan & Gullan, Planococcus citri (Risso), Pseudococcus annonae sp. n. Pacheco da Silva & Kaydan and Pseudococcus sp. were found only on sugar apple . The species F. cristinae, F. dasylirii, F. malvastra and Ph. solenopsis are recorded infesting these hosts for the first time. The most abundant mealybug species found were F. virgata , Pl. minor, Ps. jackbeardsleyi and M. hirsutus , often forming heavy infestations and damaging the fruits. A new species, Pseudococcus annonae sp. n. Pacheco da Silva & Kaydan, is described and illustrated, and an identification key to the mealybug genera occurring on annonaceous species in the Neotropical region is also provided.
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Investigation on scale insects (Hemiptera: Coccomorpha) on ornamental plants in Kayseri province
2019Co-Authors: Uğur Develioğlu, Murat Muştu, Mehmet Bora KaydanAbstract:Bu calisma 2013-2015 yillari arasinda Kayseri ilcelerinde park, yol agaclandirmasi ve peyzaj alanlarindaki sus bitkilerinde bulunan Coccomorpha (Coccidae, Diaspididae and Pseudococcidae) turlerinin saptanmasi amaciyla yurutulmustur. Arastirma sonucunda, Coccidae familyasindan 7, Diaspididae familyasindan 6 ve Pseudococcidae familyasindan 2 olmak uzere toplam 15 tur saptanmistir. Bu turler Eulecanium ciliatum (Douglas), Eulecanium tiliae (Linnaeus), Parthenolecanium corni (Bouche), Parthenolecanium persicae (Fabricius), Physokermes piceae (Schrank), Pulvinaria vitis (Linnaeus), Sphaerolecanium prunastri (Fonscolombe), Chionaspis salicis (Linnaeus), Diaspidiotus armenicus (Borchsenius), Diaspidiotus ostreaeformis (Curtis), Lepidosaphes malicola (Borchsenius), Pseudaulacaspis pentagona (Targioni-Tozzetti), Unaspis euonymi (Comstock), Planococcus vovae (Nasanov), Pseudococcus comstocki (Kuwana)’dir.
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Investigation on scale insects (Hemiptera: Coccomorpha) on ornamental plants in Kayseri province
2019Co-Authors: Uğur Develioğlu, Murat Muştu, Mehmet Bora KaydanAbstract:Bu calisma 2013-2015 yillari arasinda Kayseri ilcelerinde park, yol agaclandirmasi ve peyzaj alanlarindaki sus bitkilerinde bulunan Coccomorpha (Coccidae, Diaspididae and Pseudococcidae) turlerinin saptanmasi amaciyla yurutulmustur. Arastirma sonucunda, Coccidae familyasindan 7, Diaspididae familyasindan 6 ve Pseudococcidae familyasindan 2 olmak uzere toplam 15 tur saptanmistir. Bu turler Eulecanium ciliatum (Douglas), Eulecanium tiliae (Linnaeus), Parthenolecanium corni (Bouche), Parthenolecanium persicae (Fabricius), Physokermes piceae (Schrank), Pulvinaria vitis (Linnaeus), Sphaerolecanium prunastri (Fonscolombe), Chionaspis salicis (Linnaeus), Diaspidiotus armenicus (Borchsenius), Diaspidiotus ostreaeformis (Curtis), Lepidosaphes malicola (Borchsenius), Pseudaulacaspis pentagona (Targioni-Tozzetti), Unaspis euonymi (Comstock), Planococcus vovae (Nasanov), Pseudococcus comstocki (Kuwana)’dir.
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Integrative taxonomy methods reveal high mealybug (Hemiptera: Pseudococcidae) diversity in southern Brazilian fruit crops
Scientific Reports, 2017Co-Authors: Vitor C. Pacheco Da Silva, Jean-françois Germain, Mehmet Bora Kaydan, Ferran Palero, Thibaut Malausa, Marcos BottonAbstract:The Serra Gaúcha region is the most important temperate fruit-producing area in southern Brazil. Despite mealybugs (Hemiptera: Pseudococcidae) infesting several host plants in the region, there is a lack of information about the composition of species damaging different crops. A survey of mealybug species associated with commercial fruit crops (apple, persimmon, strawberry and grapes) was performed in Serra Gaúcha between 2013 and 2015, using both morphology and DNA analyses for species identification. The most abundant species were Pseudococcus viburni (Signoret), found on all four host plant species, and Dysmicoccus brevipes (Cockerell), infesting persimmon, vines and weeds. The highest diversity of mealybug species was found on persimmon trees, hosting 20 different taxa, of which Anisococcus granarae Pacheco da Silva & Kaydan, D . brevipes , Pseudococcus sociabilis Hambleton and Ps . viburni were the most abundant. A total of nine species were recorded in vineyards. Planococcus ficus (Signoret) and Pseudococcus longispinus (Targioni Tozzetti) were observed causing damage to grapes for the first time. A single species, Ps . viburni , was found associated with apples, while both Ps . viburni and Ferrisia meridionalis Williams were found on strawberry. Four of the mealybug species found represent new records for Brazil.
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Investigation of mealybug (hemiptera: Coccoidea: Pseudococcidae) species from Turkey by RAPD
Phytoparasitica, 2007Co-Authors: Ç. Ulubaş Serçe, A. N. Kilinçer, Mehmet Bora Kaydan, F. ErtunçAbstract:Molecular markers are commonly used for determining the relationship between insect species. In this study, we investigated the relationship of six mealybug species, Planococcus citri (Risso), Planococcus ficus (Signoret), Planococcus vovae (Nasonov), Pseudococcus longispinus (Targioni Tozzetti), Pseudococcus viburni (Signoret) and Phenacoccus aceris (Signoret), sampled from Turkey, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Of the 50 RAPD primers tested, 18 identified a total of 256 fragments of which all were polymorphic. Sufficient discrimination between P. citri and P. ficus was provided by several RAPD primers. The genetic distance values calculated for each pair-wise comparison ranged from 0.156 to 0.504. Cluster analyses of RAPD data clearly separated the species into two groups.
