The Experts below are selected from a list of 171 Experts worldwide ranked by ideXlab platform

Shoufeng Zhang - One of the best experts on this subject based on the ideXlab platform.

  • prevalence and genetic characterization of toxoplasma gondii in badgers Melogale moschata in southern china by pcr rflp
    Infection Genetics and Evolution, 2017
    Co-Authors: Kai Chen, Shoufeng Zhang, Siyang Huang, Jinlei Wang, Qiuxia Yao, Xingquan Zhu, Quan Liu
    Abstract:

    Toxoplasma gondii is an obligate intracellular apicomplexan parasite which is able to infect almost all warm-blooded animals. There is no information about the prevalence and genetic characterization of T. gondii in badgers (Melogale moschata) in China. Here, a total of 367 badgers were captured from different cities in Jiangxi province, Southern China. Genomic DNA was extracted from brain tissues of each badgers, and 57 (15.45%) of them were positive for T. gondii by semi-nested PCR of the B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci (SAG1, 5'-SAG2 and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1) and an apicoplast locus (Apico), with multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Among them, 4 were completely typed at all loci, and 2 was genotyped for 9 loci, showing that they belong to ToxoDB#9. This is the first report of prevalence and genetic characterization of T. gondii isolates from badgers in China, which contributes to broader understanding of population structure of T. gondii in China. It is important for the prevention and control of T. gondii infection in wild animals.

  • Novel calicivirus from a ferret badger (Melogale moschata) in China
    Archives of Virology, 2015
    Co-Authors: Faming Miao, Yue-hong Li, Shoufeng Zhang, Fu-chun Miao, Jinghui Zhao, Rongliang Hu
    Abstract:

    We describe the isolation and complete genome sequence of a new calicivirus, FBCV-JX12, isolated from a ferret badger (Melogale moschata). Comparison of FBCV-JX12 with other vesiviruses revealed that it shared the highest amino acid sequence identities of 71.6, 60.5, and 59.3 % in the nonstructural protein, VP1, and VP2, respectively, with MCV-DL2007 (mink calicivirus). Phylogenetic analysis of the whole genomic sequence showed that it clustered most closely with MCV-DL2007 of the genus Vesivirus, but with low nucleotide similarity in the three open reading frames (62.1-68.5 %).

  • experimental oral immunization of ferret badgers Melogale moschata with a recombinant canine adenovirus vaccine cav 2 e3δ rgp and an attenuated rabies virus srv9
    Journal of Wildlife Diseases, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Lijun Fang, Fei Zhang
    Abstract:

    Abstract Ferret badgers (Melogale moschata) are a major reservoir of rabies virus in southeastern China. Oral immunization has been shown to be a practical method for wildlife rabies management in Europe and North America. Two groups of 20 ferret badgers were given a single oral dose of a recombinant canine adenovirus-rabies vaccine, CAV-2-E3Δ-RGP, or an experimental attenuated rabies virus vaccine, SRV9. At 21 days, all ferret badgers had seroconverted, with serum virus-neutralizing antibodies ranging from 0.1 to 4.5 IU/mL. Titers were >0.50 IU/mL (an acceptable level) in 17/20 and 16/20 animals receiving CAV-2-E3Δ-RGP or SRV9, respectively. The serologic results indicate that the recombinant CAV-2-E3Δ-RGP is at least as effective as the attenuated rabies virus vaccine. Both may be considered for additional research as oral rabies vaccine candidates for ferret badgers.

  • molecular characterization of three ferret badger Melogale moschata rabies virus isolates from jiangxi province china
    Archives of Virology, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ying Wang, Ye Liu, Fei Zhang, Shuchao Wang
    Abstract:

    Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

  • complete genome sequence of a rabies virus isolate from a ferret badger Melogale moschata in jiangxi china
    Genome Announcements, 2013
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Fei Zhang
    Abstract:

    The genome of ferret badger rabies virus JX09-17(fb), isolated in Jiangxi Province, China, in 2009 has been completely sequenced. The genomic length is 11, 923 nucleotides (nt) with an overall organization similar to that of other rabies virus isolates. JX09-17(fb) is closely related to Chinese epidemic canine isolates in clade I.

Fei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • experimental oral immunization of ferret badgers Melogale moschata with a recombinant canine adenovirus vaccine cav 2 e3δ rgp and an attenuated rabies virus srv9
    Journal of Wildlife Diseases, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Lijun Fang, Fei Zhang
    Abstract:

    Abstract Ferret badgers (Melogale moschata) are a major reservoir of rabies virus in southeastern China. Oral immunization has been shown to be a practical method for wildlife rabies management in Europe and North America. Two groups of 20 ferret badgers were given a single oral dose of a recombinant canine adenovirus-rabies vaccine, CAV-2-E3Δ-RGP, or an experimental attenuated rabies virus vaccine, SRV9. At 21 days, all ferret badgers had seroconverted, with serum virus-neutralizing antibodies ranging from 0.1 to 4.5 IU/mL. Titers were >0.50 IU/mL (an acceptable level) in 17/20 and 16/20 animals receiving CAV-2-E3Δ-RGP or SRV9, respectively. The serologic results indicate that the recombinant CAV-2-E3Δ-RGP is at least as effective as the attenuated rabies virus vaccine. Both may be considered for additional research as oral rabies vaccine candidates for ferret badgers.

  • molecular characterization of three ferret badger Melogale moschata rabies virus isolates from jiangxi province china
    Archives of Virology, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ying Wang, Ye Liu, Fei Zhang, Shuchao Wang
    Abstract:

    Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

  • complete genome sequence of a rabies virus isolate from a ferret badger Melogale moschata in jiangxi china
    Genome Announcements, 2013
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Fei Zhang
    Abstract:

    The genome of ferret badger rabies virus JX09-17(fb), isolated in Jiangxi Province, China, in 2009 has been completely sequenced. The genomic length is 11, 923 nucleotides (nt) with an overall organization similar to that of other rabies virus isolates. JX09-17(fb) is closely related to Chinese epidemic canine isolates in clade I.

  • epidemic and maintenance of rabies in chinese ferret badgers Melogale moschata indicated by epidemiology and the molecular signatures of rabies viruses
    Virologica Sinica, 2013
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Fei Zhang, Yanli Hou, Ying Wang
    Abstract:

    An epidemic of Chinese ferret badger-associated human rabies was investigated in Wuyuan county, Jiangxi province and rabies viruses isolates from ferret badgers in different districts in Jiangxi and Zhejiang provinces were sequenced with their nucleotides and amino acids and aligned for epidemiological analysis. The results showed that the human rabies in Wuyuan are only associated with ferret badger bites; the rabies virus can be isolated in a high percentage of ferret badgers in the epidemic areas in Jiangxi and Zhejiang provinces; the isolates share the same molecular features in nucleotides and have characteristic amino acid signatures, i.e., 2 sites in the nucleoprotein and 3 sites in the glycoprotein, that are distinct from virus isolates from dogs in the same region. We conclude that rabies in Chinese ferret badgers has formed an independent transmission cycle and ferret badgers may serve as another important rabies reservoir independent of dog rabies in China.

  • characterization of a rabies virus isolate from a ferret badger Melogale moschata with unique molecular differences in glycoprotein antigenic site iii
    Virus Research, 2010
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Anthony R Fooks, Fei Zhang
    Abstract:

    Rabies virus was isolated from the brain of a Chinese ferret badger (Melogale moschata) and identified as having an R333Q substitution within its glycoprotein antigenic site III. Additionally, compared with vaccine strains and other rabies virus isolates from dogs and ferret badgers in China, the isolate had five other amino acid substitutions in its glycoprotein: P(-17)L in the signal peptide, R88H, L225M, and D422E in the ectoplasmic region, and G478E in the cytoplasmic region. This isolate possessed high virulence in suckling, weanling and adult mice. These data indicate that this is a unique rabies virus with a molecular signature that differentiates it from other strains circulating in terrestrial mammals in China. We propose that rabies virus circulates in some ferret badgers in an independent epidemiological cycle unique to China following spillover from domestic dogs or other hosts infected with rabies.

Jinghui Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Novel calicivirus from a ferret badger (Melogale moschata) in China
    Archives of Virology, 2015
    Co-Authors: Faming Miao, Yue-hong Li, Shoufeng Zhang, Fu-chun Miao, Jinghui Zhao, Rongliang Hu
    Abstract:

    We describe the isolation and complete genome sequence of a new calicivirus, FBCV-JX12, isolated from a ferret badger (Melogale moschata). Comparison of FBCV-JX12 with other vesiviruses revealed that it shared the highest amino acid sequence identities of 71.6, 60.5, and 59.3 % in the nonstructural protein, VP1, and VP2, respectively, with MCV-DL2007 (mink calicivirus). Phylogenetic analysis of the whole genomic sequence showed that it clustered most closely with MCV-DL2007 of the genus Vesivirus, but with low nucleotide similarity in the three open reading frames (62.1-68.5 %).

  • experimental oral immunization of ferret badgers Melogale moschata with a recombinant canine adenovirus vaccine cav 2 e3δ rgp and an attenuated rabies virus srv9
    Journal of Wildlife Diseases, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Lijun Fang, Fei Zhang
    Abstract:

    Abstract Ferret badgers (Melogale moschata) are a major reservoir of rabies virus in southeastern China. Oral immunization has been shown to be a practical method for wildlife rabies management in Europe and North America. Two groups of 20 ferret badgers were given a single oral dose of a recombinant canine adenovirus-rabies vaccine, CAV-2-E3Δ-RGP, or an experimental attenuated rabies virus vaccine, SRV9. At 21 days, all ferret badgers had seroconverted, with serum virus-neutralizing antibodies ranging from 0.1 to 4.5 IU/mL. Titers were >0.50 IU/mL (an acceptable level) in 17/20 and 16/20 animals receiving CAV-2-E3Δ-RGP or SRV9, respectively. The serologic results indicate that the recombinant CAV-2-E3Δ-RGP is at least as effective as the attenuated rabies virus vaccine. Both may be considered for additional research as oral rabies vaccine candidates for ferret badgers.

  • molecular characterization of three ferret badger Melogale moschata rabies virus isolates from jiangxi province china
    Archives of Virology, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ying Wang, Ye Liu, Fei Zhang, Shuchao Wang
    Abstract:

    Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

  • complete genome sequence of a rabies virus isolate from a ferret badger Melogale moschata in jiangxi china
    Genome Announcements, 2013
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Fei Zhang
    Abstract:

    The genome of ferret badger rabies virus JX09-17(fb), isolated in Jiangxi Province, China, in 2009 has been completely sequenced. The genomic length is 11, 923 nucleotides (nt) with an overall organization similar to that of other rabies virus isolates. JX09-17(fb) is closely related to Chinese epidemic canine isolates in clade I.

  • epidemic and maintenance of rabies in chinese ferret badgers Melogale moschata indicated by epidemiology and the molecular signatures of rabies viruses
    Virologica Sinica, 2013
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Fei Zhang, Yanli Hou, Ying Wang
    Abstract:

    An epidemic of Chinese ferret badger-associated human rabies was investigated in Wuyuan county, Jiangxi province and rabies viruses isolates from ferret badgers in different districts in Jiangxi and Zhejiang provinces were sequenced with their nucleotides and amino acids and aligned for epidemiological analysis. The results showed that the human rabies in Wuyuan are only associated with ferret badger bites; the rabies virus can be isolated in a high percentage of ferret badgers in the epidemic areas in Jiangxi and Zhejiang provinces; the isolates share the same molecular features in nucleotides and have characteristic amino acid signatures, i.e., 2 sites in the nucleoprotein and 3 sites in the glycoprotein, that are distinct from virus isolates from dogs in the same region. We conclude that rabies in Chinese ferret badgers has formed an independent transmission cycle and ferret badgers may serve as another important rabies reservoir independent of dog rabies in China.

Ying Wang - One of the best experts on this subject based on the ideXlab platform.

  • molecular characterization of three ferret badger Melogale moschata rabies virus isolates from jiangxi province china
    Archives of Virology, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ying Wang, Ye Liu, Fei Zhang, Shuchao Wang
    Abstract:

    Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

  • epidemic and maintenance of rabies in chinese ferret badgers Melogale moschata indicated by epidemiology and the molecular signatures of rabies viruses
    Virologica Sinica, 2013
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Fei Zhang, Yanli Hou, Ying Wang
    Abstract:

    An epidemic of Chinese ferret badger-associated human rabies was investigated in Wuyuan county, Jiangxi province and rabies viruses isolates from ferret badgers in different districts in Jiangxi and Zhejiang provinces were sequenced with their nucleotides and amino acids and aligned for epidemiological analysis. The results showed that the human rabies in Wuyuan are only associated with ferret badger bites; the rabies virus can be isolated in a high percentage of ferret badgers in the epidemic areas in Jiangxi and Zhejiang provinces; the isolates share the same molecular features in nucleotides and have characteristic amino acid signatures, i.e., 2 sites in the nucleoprotein and 3 sites in the glycoprotein, that are distinct from virus isolates from dogs in the same region. We conclude that rabies in Chinese ferret badgers has formed an independent transmission cycle and ferret badgers may serve as another important rabies reservoir independent of dog rabies in China.

  • some observations on the reproduction of the taiwan ferret badger Melogale moschata subaurantiaca in southern taiwan
    Zoological Studies, 1995
    Co-Authors: Kurtis Jai-chyi Pei, Ying Wang
    Abstract:

    Taiwan ferret badgers (Melogale moschata subaurantiaca) were collected every month from 1991 to 1993 in southern Taiwan with a total of 238 specimens altogether. Post-mortem examination showed that their reproductive systems are typical of the Canoideas group and are symmetrical. Most adult males produced sperm from February to September, and almost all pregnant females were found between March and October. Newly independent males were collected between August and January. A weak synchronization in females' reproductive rhythm was observed. Litter size for the species is two, and evidence suggests that they breed once a year.

Ye Liu - One of the best experts on this subject based on the ideXlab platform.

  • experimental oral immunization of ferret badgers Melogale moschata with a recombinant canine adenovirus vaccine cav 2 e3δ rgp and an attenuated rabies virus srv9
    Journal of Wildlife Diseases, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Lijun Fang, Fei Zhang
    Abstract:

    Abstract Ferret badgers (Melogale moschata) are a major reservoir of rabies virus in southeastern China. Oral immunization has been shown to be a practical method for wildlife rabies management in Europe and North America. Two groups of 20 ferret badgers were given a single oral dose of a recombinant canine adenovirus-rabies vaccine, CAV-2-E3Δ-RGP, or an experimental attenuated rabies virus vaccine, SRV9. At 21 days, all ferret badgers had seroconverted, with serum virus-neutralizing antibodies ranging from 0.1 to 4.5 IU/mL. Titers were >0.50 IU/mL (an acceptable level) in 17/20 and 16/20 animals receiving CAV-2-E3Δ-RGP or SRV9, respectively. The serologic results indicate that the recombinant CAV-2-E3Δ-RGP is at least as effective as the attenuated rabies virus vaccine. Both may be considered for additional research as oral rabies vaccine candidates for ferret badgers.

  • molecular characterization of three ferret badger Melogale moschata rabies virus isolates from jiangxi province china
    Archives of Virology, 2014
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ying Wang, Ye Liu, Fei Zhang, Shuchao Wang
    Abstract:

    Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

  • complete genome sequence of a rabies virus isolate from a ferret badger Melogale moschata in jiangxi china
    Genome Announcements, 2013
    Co-Authors: Jinghui Zhao, Shoufeng Zhang, Ye Liu, Fei Zhang
    Abstract:

    The genome of ferret badger rabies virus JX09-17(fb), isolated in Jiangxi Province, China, in 2009 has been completely sequenced. The genomic length is 11, 923 nucleotides (nt) with an overall organization similar to that of other rabies virus isolates. JX09-17(fb) is closely related to Chinese epidemic canine isolates in clade I.

  • epidemic and maintenance of rabies in chinese ferret badgers Melogale moschata indicated by epidemiology and the molecular signatures of rabies viruses
    Virologica Sinica, 2013
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Fei Zhang, Yanli Hou, Ying Wang
    Abstract:

    An epidemic of Chinese ferret badger-associated human rabies was investigated in Wuyuan county, Jiangxi province and rabies viruses isolates from ferret badgers in different districts in Jiangxi and Zhejiang provinces were sequenced with their nucleotides and amino acids and aligned for epidemiological analysis. The results showed that the human rabies in Wuyuan are only associated with ferret badger bites; the rabies virus can be isolated in a high percentage of ferret badgers in the epidemic areas in Jiangxi and Zhejiang provinces; the isolates share the same molecular features in nucleotides and have characteristic amino acid signatures, i.e., 2 sites in the nucleoprotein and 3 sites in the glycoprotein, that are distinct from virus isolates from dogs in the same region. We conclude that rabies in Chinese ferret badgers has formed an independent transmission cycle and ferret badgers may serve as another important rabies reservoir independent of dog rabies in China.

  • characterization of a rabies virus isolate from a ferret badger Melogale moschata with unique molecular differences in glycoprotein antigenic site iii
    Virus Research, 2010
    Co-Authors: Shoufeng Zhang, Jinghui Zhao, Ye Liu, Anthony R Fooks, Fei Zhang
    Abstract:

    Rabies virus was isolated from the brain of a Chinese ferret badger (Melogale moschata) and identified as having an R333Q substitution within its glycoprotein antigenic site III. Additionally, compared with vaccine strains and other rabies virus isolates from dogs and ferret badgers in China, the isolate had five other amino acid substitutions in its glycoprotein: P(-17)L in the signal peptide, R88H, L225M, and D422E in the ectoplasmic region, and G478E in the cytoplasmic region. This isolate possessed high virulence in suckling, weanling and adult mice. These data indicate that this is a unique rabies virus with a molecular signature that differentiates it from other strains circulating in terrestrial mammals in China. We propose that rabies virus circulates in some ferret badgers in an independent epidemiological cycle unique to China following spillover from domestic dogs or other hosts infected with rabies.