The Experts below are selected from a list of 172188 Experts worldwide ranked by ideXlab platform
Hiran Perinpanayagam - One of the best experts on this subject based on the ideXlab platform.
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titania polymeric powder coatings with nano topography support enhanced human Mesenchymal Cell responses
Journal of Biomedical Materials Research Part A, 2012Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Titanium implant osseointegration is dependent on the Cellular response to surface modifications and coatings. Titania-enriched nanocomposite polymeric resin coatings were prepared through the application of advanced ultrafine powder coating technology. Their surfaces were readily modified to create nano-rough (<100 nm) surface nano-topographies that supported human embryonic palatal Mesenchymal Cell responses. Energy dispersive x-ray spectroscopy confirmed continuous and homogenous coatings with a similar composition and even distribution of titanium. Scanning electron microscopy (SEM) showed complex micro-topographies, and atomic force microscopy revealed intricate nanofeatures and surface roughness. Cell counts, mitochondrial enzyme activity reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to dark purple, SEM, and inverted fluorescence microscopy showed a marked increase in Cell attachment, spreading, proliferation, and metabolic activity on the nanostructured surfaces. Reverse Transcription- Polymerase Chain Reaction (RT-PCR) analysis showed that type I collagen and Runx2 expression were induced, and Alizarin red staining showed that mineral deposits were abundant in the Cell cultures grown on nanosurfaces. This enhancement in human Mesenchymal Cell attachment, growth, and osteogenesis were attributed to the nanosized surface topographies, roughness, and moderate wetting characteristics of the coatings. Their dimensional similarity to naturally occurring matrix proteins and crystals, coupled with their increased surface area for protein adsorption, may have facilitated the response. Therefore, this application of ultrafine powder coating technology affords highly biocompatible surfaces that can be readily modified to accentuate the Cellular response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2695–2709, 2012.
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mta enriched nanocomposite tio 2 polymeric powder coatings support human Mesenchymal Cell attachment and growth
Biomedical Materials, 2012Co-Authors: Wen Shi, Jesse Zhu, Hui Zhang, Mohammad Sayem Mozumder, Hiran PerinpanayagamAbstract:The objective of the study described in this paper was the development of novel polymer/ceramic nanocomposite coatings for implants through the application of ultrafine powder coating technology. Polyester resins were combined with µm-sized TiO(2) (25%) as the biocompatibility agent, nTiO(2) (0.5%) as the flow additive and mineral trioxide aggregates (ProRoot® MTA, 5%) as bioactive ceramics. Ultrafine powders were prepared and applied to titanium to create continuous polymeric powder coatings (PPCs) through the application of electrostatic ultrafine powder coating technology. Energy dispersive x-ray analysis confirmed that MTA had been incorporated into the PPCs, and elemental mapping showed that it had formed small clusters that were evenly distributed across the surface. Scanning electron microscopy (SEM) revealed continuous and smooth, but highly textured surface coatings that contrasted with the scalloped appearance of commercially pure titanium (cpTi) controls. Atomic force microscopy revealed intricate nano-topographies with an abundance of submicron-sized pits and nano-projections, evenly dispersed across their surfaces. Inverted fluorescence microscopy, SEM and Cell counts showed that human embryonic palatal Mesenchymal Cells attached and spread out onto PPC and MTA-enriched PPCs within 24 h. Mitochondrial enzyme activity measured viable and metabolically active Cells on all of the surfaces. After 72 h of growth, Cell counts and metabolic activity were significantly higher (P < 0.05) on the grey-MTA enriched PPC surfaces, than on unmodified PPC and cpTi. The novel polymer/ceramic nanocomposites that were created with ultrafine powder coating technology were continuous, homogenous and nano-rough coatings that enhanced human Mesenchymal Cell attachment and growth.
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nano tio 2 enriched polymeric powder coatings support human Mesenchymal Cell attachment and growth
Journal of Biomaterials Applications, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:The objective of this study was to utilize ultrafine powder coating technology to prepare (PPC) that can support human Mesenchymal Cell attachment and growth. Resins were modified with titanium dioxide and polytetrafluoroethylene (PTFE), and enriched with either SiO2 or TiO2 nanoparticles (nSiO2 or nTiO2) to create continuous PPC. Scanning electron microscopy (SEM) revealed complex surface topographies with nano features, and energy dispersive X-ray (EDX) analysis with Ti mapping confirmed a homogenous dispersion of the material. SEM and inverted fluorescence microscopy showed that human embryonic palatal Mesenchymal (HEPM) Cells attached and spread out on the PPC surfaces, particularly those enriched with nTiO 2. Cell counts were higher, and the MTT assay measured more metabolic activity from the nTiO2 enriched PPCs. Furthermore, these Cellular responses were enhanced on PPC surfaces that were enriched with a higher concentration of nTiO2 (2% vs. 0.5%), and appeared comparable to that seen on commerciall...
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TiO2-enriched polymeric powder coatings support human Mesenchymal Cell spreading and osteogenic differentiation
Biomedical Materials, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Novel polymeric powder coatings (PPC) were prepared by ultrafine powder coating technology and shown to support human Mesenchymal Cell attachment and growth. PPC surfaces enriched with nano-TiO2 (nTiO2) showed enhanced Cellular responses, and were compared to commercially pure titanium (cpTi). After Cell attachment and growth, osteogenic differentiation and bone matrix formation ensures osseointegration for implantable biomaterials. Therefore, the objective of this study was to determine if Mesenchymal Cells grown on PPC could undergo osteogenic differentiation by inducing Runx2 and bone matrix proteins, and then initiate mineralization. Atomic force microscopy revealed intricate three-dimensional micro-topographies, and the measures of nano-roughness and porosity were similar for all PPC surfaces. Scanning electron microscopy showed that the Cells attached and spread out over all of the surfaces. After 1 week in osteogenic media, RT-PCR analysis showed the induction of Runx2, the up-regulation of type I collagen, and the initial detection of alkaline phosphatase and bone sialoprotein. After 4 weeks, Alizarin Red staining showed mineral deposition. However, Cell spreading and osteogenic differentiation were significantly (P < 0.05) higher on the cpTi controls than on the PPC surfaces. Furthermore, spreading and differentiation were consistently higher on the titanium-enriched PPC-2, -3 and -4 than on the titanium-free PPC-1. Therefore, despite the presence of complex micro-topographies and nano-features, titanium-enrichment enhanced the Cellular response, and pure titanium still provided the best substrate. These findings confirm the cytocompatibility of these novel polymeric coatings and suggest that titanium-enrichment and nTiO2 additives may enhance their performance.
Junsheng Feng - One of the best experts on this subject based on the ideXlab platform.
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dentin sialoprotein facilitates dental Mesenchymal Cell differentiation and dentin formation
Scientific Reports, 2017Co-Authors: Lei Chen, Mary Macdougall, Junsheng Feng, Kevin J Donly, Zhuo Chen, Feng Wang, Lisa Shoff, Shuo ChenAbstract:Dentin sialoprotein (DSP) is a dentin extraCellular matrix protein. It is involved in dental Mesenchymal Cell lineages and dentin formation through regulation of its target gene expression. DSP mutations cause dentin genetic diseases. However, mechanisms of DSP in controlling dental Mesenchymal Cell differentiation are unknown. Using DSP as bait, we screened a protein library from mouse odontoblastic Cells and found that DSP is a ligand and binds to Cell surface receptor, occludin. Further study identified that the C-terminal DSP domainaa 363–458 interacts with the occludin extraCellular loop 2aa 194–241. The C-terminal DSP domain induced phosphorylation of occludin Ser490 and focal adhesion kinase (FAK) Ser722 and Tyr576. Coexpression of DSP, occludin and FAK was detected in dental Mesenchymal Cells during tooth development. Occludin physically interacts with FAK, and occludin and FAK phosphorylation can be blocked by DSP and occludin antibodies. This DSP domain facilitates dental Mesenchymal Cell differentiation and mineralization. Furthermore, transplantation and pulp-capping procedures revealed that this DSP domain induces endogenous dental pulp Mesenchymal Cell proliferation, differentiation and migration, while stimulating blood vessel proliferation. This study elucidates the mechanism of DSP in dental Mesenchymal lineages and implies that DSP may serve as a therapeutic agent for dentin-pulp complex regeneration in dental caries.
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immortalized mouse floxed bmp2 dental papilla Mesenchymal Cell lines preserve odontoblastic phenotype and respond to bmp2
Journal of Cellular Physiology, 2010Co-Authors: Li An Wu, Junsheng Feng, Lynn Wang, Yan Dong Mu, Andrew Baker, Kevin J Donly, Jelica Gluhakheinrich, S E HarrisAbstract:Tooth development involves sequential and reciprocal interactions between dental epithelial and Mesenchymal Cells, and proceeds through a series of cytodifferentiations in specific spatial-temporal patterns (Linde and Goldberg, 1993). Dentinogenesis is a complex process in which multiple signaling pathways converge to induce dentin formation and is controlled by many growth and transcription factors (Thesleff, 2003). The bone morphogenetic proteins (Bmps) are structurally related to the transforming growth factor beta (TGF-β) superfamily and were originally identified by their capacity to induce ectopic bone formation in rodents (Urist, 1965; Wozney et al., 1988). Members of the Bmp family have diverse biological functions during embryonic development (Hogan, 1996; Wu et al., 2003) including a vital role in osteogenesis (Chen et al., 2004; Rosen, 2009). Among the Bmp family members, Bmp2 has been extensively studied for its various biological functions during chondrogenic and osteogenic differentiation (Reddi, 1997; Ducy and Karsenty, 2000). Also, Bmp2 has been shown to promote dental pulp stem Cell commitment to the odontoblast lineage in vitro (Yang et al., 2009) and induces dental pulp Cell differentiation and mineralization in vitro and in vivo (Nakashima, 2005; Chen et al., 2008). However, detail understandings of the molecular mechanisms of Bmp2 exerting its effects on tooth development and formation remain elusive in particular during postnatal tooth development as homozygous mutant embryos for Bmp2 show developmental abnormalities and die at embryo day 9.5 (Zhang and Bradley, 1996). Recently, conditional Bmp2 knock out (cBmp2-KO) mice were generated and revealed important roles of Bmp2 in later stages of osteogenesis (Bandyopadhyay et al., 2006) and bone fracture healing (Tsuji et al., 2006) as well as other organ development (Ma et al., 2005; Rivera-Feliciano and Tabin, 2006; Lee et al., 2007; Singh et al., 2008). However, roles of Bmp2 during tooth development and formation have not been completely understood. Unlike bone and other tissues, it is relatively hard to collect enough amounts of dental tissues from a single tooth. Therefore, generation of a floxed Bmp2 dental papilla Mesenchymal Cell line would be a valuable tool for studying the effects of Bmp2 on dental Cell lineages as well as relevant molecular events involved in matrix mineralization and dentin regeneration. Such information will help realize the potential of BMP2 as therapeutic agent and for the rational targeting of specific Bmp2 to the appropriate clinical indication. In this study, we established an immortalized mouse floxed Bmp2 dental papilla Mesenchymal Cell line using transduction of simian phenotypic and virus 40 T-antigen (SV40). We further observed these Cell growth rates and their genotypic and phenotypic characteristics as compared to primary Cells. Finally, we tested whether these immortalized Cells were inducible by growth factors.
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immortalized mouse floxed bmp2 dental papilla Mesenchymal Cell lines preserve odontoblastic phenotype and respond to bmp2
Journal of Cellular Physiology, 2010Co-Authors: Mary Macdougall, Junsheng Feng, Lynn Wang, Andrew Baker, Kevin J Donly, Jelica Gluhakheinrich, S E Harris, Shuo ChenAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for odontogensis and dentin mineralization. Generation of floxed Bmp2 dental Mesenchymal Cell lines is a valuable application for studying the effects of Bmp2 on dental Mesenchymal Cell differentiation and its signaling pathways during dentinogenesis. Limitation of the primary culture of dental Mesenchymal Cells has led to the development of Cell lines that serve as good surrogate models for the study of dental Mesenchymal Cell differentiation into odontoblasts and mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 dental papilla Mesenchymal Cell lines, which were isolated from 1st mouse mandibular molars at postnatal day 1 and immortalized with pSV40 and clonally selected. These transfected Cell lines were characterized by RT-PCR, immunohistochemistry, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized Cell lines, iBmp2-dp, displayed a higher proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary Cells as determined by expression of tooth-specific markers as well as demonstrated the ability to differentiate and form mineralized nodules. In addition, iBmp2-dp Cells were inducible and responded to BMP2 stimulation. Thus, we for the first time described the establishment of an immortalized mouse floxed Bmp2 dental papilla mesenchyma Cell line that might be used for studying the mechanisms of dental Cell differentiation and dentin mineralization mediated by Bmp2 and other growth factor signaling pathways.
Mohammad Sayem Mozumder - One of the best experts on this subject based on the ideXlab platform.
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titania polymeric powder coatings with nano topography support enhanced human Mesenchymal Cell responses
Journal of Biomedical Materials Research Part A, 2012Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Titanium implant osseointegration is dependent on the Cellular response to surface modifications and coatings. Titania-enriched nanocomposite polymeric resin coatings were prepared through the application of advanced ultrafine powder coating technology. Their surfaces were readily modified to create nano-rough (<100 nm) surface nano-topographies that supported human embryonic palatal Mesenchymal Cell responses. Energy dispersive x-ray spectroscopy confirmed continuous and homogenous coatings with a similar composition and even distribution of titanium. Scanning electron microscopy (SEM) showed complex micro-topographies, and atomic force microscopy revealed intricate nanofeatures and surface roughness. Cell counts, mitochondrial enzyme activity reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to dark purple, SEM, and inverted fluorescence microscopy showed a marked increase in Cell attachment, spreading, proliferation, and metabolic activity on the nanostructured surfaces. Reverse Transcription- Polymerase Chain Reaction (RT-PCR) analysis showed that type I collagen and Runx2 expression were induced, and Alizarin red staining showed that mineral deposits were abundant in the Cell cultures grown on nanosurfaces. This enhancement in human Mesenchymal Cell attachment, growth, and osteogenesis were attributed to the nanosized surface topographies, roughness, and moderate wetting characteristics of the coatings. Their dimensional similarity to naturally occurring matrix proteins and crystals, coupled with their increased surface area for protein adsorption, may have facilitated the response. Therefore, this application of ultrafine powder coating technology affords highly biocompatible surfaces that can be readily modified to accentuate the Cellular response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2695–2709, 2012.
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mta enriched nanocomposite tio 2 polymeric powder coatings support human Mesenchymal Cell attachment and growth
Biomedical Materials, 2012Co-Authors: Wen Shi, Jesse Zhu, Hui Zhang, Mohammad Sayem Mozumder, Hiran PerinpanayagamAbstract:The objective of the study described in this paper was the development of novel polymer/ceramic nanocomposite coatings for implants through the application of ultrafine powder coating technology. Polyester resins were combined with µm-sized TiO(2) (25%) as the biocompatibility agent, nTiO(2) (0.5%) as the flow additive and mineral trioxide aggregates (ProRoot® MTA, 5%) as bioactive ceramics. Ultrafine powders were prepared and applied to titanium to create continuous polymeric powder coatings (PPCs) through the application of electrostatic ultrafine powder coating technology. Energy dispersive x-ray analysis confirmed that MTA had been incorporated into the PPCs, and elemental mapping showed that it had formed small clusters that were evenly distributed across the surface. Scanning electron microscopy (SEM) revealed continuous and smooth, but highly textured surface coatings that contrasted with the scalloped appearance of commercially pure titanium (cpTi) controls. Atomic force microscopy revealed intricate nano-topographies with an abundance of submicron-sized pits and nano-projections, evenly dispersed across their surfaces. Inverted fluorescence microscopy, SEM and Cell counts showed that human embryonic palatal Mesenchymal Cells attached and spread out onto PPC and MTA-enriched PPCs within 24 h. Mitochondrial enzyme activity measured viable and metabolically active Cells on all of the surfaces. After 72 h of growth, Cell counts and metabolic activity were significantly higher (P < 0.05) on the grey-MTA enriched PPC surfaces, than on unmodified PPC and cpTi. The novel polymer/ceramic nanocomposites that were created with ultrafine powder coating technology were continuous, homogenous and nano-rough coatings that enhanced human Mesenchymal Cell attachment and growth.
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nano tio 2 enriched polymeric powder coatings support human Mesenchymal Cell attachment and growth
Journal of Biomaterials Applications, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:The objective of this study was to utilize ultrafine powder coating technology to prepare (PPC) that can support human Mesenchymal Cell attachment and growth. Resins were modified with titanium dioxide and polytetrafluoroethylene (PTFE), and enriched with either SiO2 or TiO2 nanoparticles (nSiO2 or nTiO2) to create continuous PPC. Scanning electron microscopy (SEM) revealed complex surface topographies with nano features, and energy dispersive X-ray (EDX) analysis with Ti mapping confirmed a homogenous dispersion of the material. SEM and inverted fluorescence microscopy showed that human embryonic palatal Mesenchymal (HEPM) Cells attached and spread out on the PPC surfaces, particularly those enriched with nTiO 2. Cell counts were higher, and the MTT assay measured more metabolic activity from the nTiO2 enriched PPCs. Furthermore, these Cellular responses were enhanced on PPC surfaces that were enriched with a higher concentration of nTiO2 (2% vs. 0.5%), and appeared comparable to that seen on commerciall...
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TiO2-enriched polymeric powder coatings support human Mesenchymal Cell spreading and osteogenic differentiation
Biomedical Materials, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Novel polymeric powder coatings (PPC) were prepared by ultrafine powder coating technology and shown to support human Mesenchymal Cell attachment and growth. PPC surfaces enriched with nano-TiO2 (nTiO2) showed enhanced Cellular responses, and were compared to commercially pure titanium (cpTi). After Cell attachment and growth, osteogenic differentiation and bone matrix formation ensures osseointegration for implantable biomaterials. Therefore, the objective of this study was to determine if Mesenchymal Cells grown on PPC could undergo osteogenic differentiation by inducing Runx2 and bone matrix proteins, and then initiate mineralization. Atomic force microscopy revealed intricate three-dimensional micro-topographies, and the measures of nano-roughness and porosity were similar for all PPC surfaces. Scanning electron microscopy showed that the Cells attached and spread out over all of the surfaces. After 1 week in osteogenic media, RT-PCR analysis showed the induction of Runx2, the up-regulation of type I collagen, and the initial detection of alkaline phosphatase and bone sialoprotein. After 4 weeks, Alizarin Red staining showed mineral deposition. However, Cell spreading and osteogenic differentiation were significantly (P < 0.05) higher on the cpTi controls than on the PPC surfaces. Furthermore, spreading and differentiation were consistently higher on the titanium-enriched PPC-2, -3 and -4 than on the titanium-free PPC-1. Therefore, despite the presence of complex micro-topographies and nano-features, titanium-enrichment enhanced the Cellular response, and pure titanium still provided the best substrate. These findings confirm the cytocompatibility of these novel polymeric coatings and suggest that titanium-enrichment and nTiO2 additives may enhance their performance.
Shuo Chen - One of the best experts on this subject based on the ideXlab platform.
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dentin sialoprotein facilitates dental Mesenchymal Cell differentiation and dentin formation
Scientific Reports, 2017Co-Authors: Lei Chen, Mary Macdougall, Junsheng Feng, Kevin J Donly, Zhuo Chen, Feng Wang, Lisa Shoff, Shuo ChenAbstract:Dentin sialoprotein (DSP) is a dentin extraCellular matrix protein. It is involved in dental Mesenchymal Cell lineages and dentin formation through regulation of its target gene expression. DSP mutations cause dentin genetic diseases. However, mechanisms of DSP in controlling dental Mesenchymal Cell differentiation are unknown. Using DSP as bait, we screened a protein library from mouse odontoblastic Cells and found that DSP is a ligand and binds to Cell surface receptor, occludin. Further study identified that the C-terminal DSP domainaa 363–458 interacts with the occludin extraCellular loop 2aa 194–241. The C-terminal DSP domain induced phosphorylation of occludin Ser490 and focal adhesion kinase (FAK) Ser722 and Tyr576. Coexpression of DSP, occludin and FAK was detected in dental Mesenchymal Cells during tooth development. Occludin physically interacts with FAK, and occludin and FAK phosphorylation can be blocked by DSP and occludin antibodies. This DSP domain facilitates dental Mesenchymal Cell differentiation and mineralization. Furthermore, transplantation and pulp-capping procedures revealed that this DSP domain induces endogenous dental pulp Mesenchymal Cell proliferation, differentiation and migration, while stimulating blood vessel proliferation. This study elucidates the mechanism of DSP in dental Mesenchymal lineages and implies that DSP may serve as a therapeutic agent for dentin-pulp complex regeneration in dental caries.
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immortalized mouse dental papilla Mesenchymal Cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2
In Vitro Cellular & Developmental Biology – Animal, 2013Co-Authors: Feng Wang, Lisa Shoff, Yuan Yang, Feng Guo, Qingping Gao, Huihsiu Chuang, Weiwei Wang, Shuo ChenAbstract:Odontogenesis is the result of the reciprocal interactions between epithelial–Mesenchymal Cells leading to terminally differentiated odontoblasts. This process from dental papilla Mesenchymal Cells to odontoblasts is regulated by a complex signaling pathway. When isolated from the developing tooth germs, odontoblasts quickly lose their potential to maintain the odontoblast-specific phenotype. Therefore, generation of an odontoblast-like Cell line would be a good surrogate model for studying the dental Mesenchymal Cell differentiation into odontoblasts and the molecular events of dentin formation. In this study, immortalized dental papilla Mesenchymal Cell lines were generated from the first mouse mandibular molars at postnatal day 3 using pSV40. These transformed Cells were characterized by RT-PCR, immunohistochemistry, Western blot, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized Cell lines, iMDP-3, displayed a high proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary Cells as determined by expression of tooth-specific markers and demonstrated the ability to differentiate and form mineralized nodules. Furthermore, iMDP-3 Cells had high transfection efficiency as well as were inducible and responded to BMP2 stimulation. We conclude that the establishment of the stable murine dental papilla Mesenchymal Cell line might be used for studying the mechanisms of dental Cell differentiation and dentin formation.
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immortalized mouse floxed bmp2 dental papilla Mesenchymal Cell lines preserve odontoblastic phenotype and respond to bmp2
Journal of Cellular Physiology, 2010Co-Authors: Mary Macdougall, Junsheng Feng, Lynn Wang, Andrew Baker, Kevin J Donly, Jelica Gluhakheinrich, S E Harris, Shuo ChenAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for odontogensis and dentin mineralization. Generation of floxed Bmp2 dental Mesenchymal Cell lines is a valuable application for studying the effects of Bmp2 on dental Mesenchymal Cell differentiation and its signaling pathways during dentinogenesis. Limitation of the primary culture of dental Mesenchymal Cells has led to the development of Cell lines that serve as good surrogate models for the study of dental Mesenchymal Cell differentiation into odontoblasts and mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 dental papilla Mesenchymal Cell lines, which were isolated from 1st mouse mandibular molars at postnatal day 1 and immortalized with pSV40 and clonally selected. These transfected Cell lines were characterized by RT-PCR, immunohistochemistry, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized Cell lines, iBmp2-dp, displayed a higher proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary Cells as determined by expression of tooth-specific markers as well as demonstrated the ability to differentiate and form mineralized nodules. In addition, iBmp2-dp Cells were inducible and responded to BMP2 stimulation. Thus, we for the first time described the establishment of an immortalized mouse floxed Bmp2 dental papilla mesenchyma Cell line that might be used for studying the mechanisms of dental Cell differentiation and dentin mineralization mediated by Bmp2 and other growth factor signaling pathways.
Jesse Zhu - One of the best experts on this subject based on the ideXlab platform.
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titania polymeric powder coatings with nano topography support enhanced human Mesenchymal Cell responses
Journal of Biomedical Materials Research Part A, 2012Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Titanium implant osseointegration is dependent on the Cellular response to surface modifications and coatings. Titania-enriched nanocomposite polymeric resin coatings were prepared through the application of advanced ultrafine powder coating technology. Their surfaces were readily modified to create nano-rough (<100 nm) surface nano-topographies that supported human embryonic palatal Mesenchymal Cell responses. Energy dispersive x-ray spectroscopy confirmed continuous and homogenous coatings with a similar composition and even distribution of titanium. Scanning electron microscopy (SEM) showed complex micro-topographies, and atomic force microscopy revealed intricate nanofeatures and surface roughness. Cell counts, mitochondrial enzyme activity reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to dark purple, SEM, and inverted fluorescence microscopy showed a marked increase in Cell attachment, spreading, proliferation, and metabolic activity on the nanostructured surfaces. Reverse Transcription- Polymerase Chain Reaction (RT-PCR) analysis showed that type I collagen and Runx2 expression were induced, and Alizarin red staining showed that mineral deposits were abundant in the Cell cultures grown on nanosurfaces. This enhancement in human Mesenchymal Cell attachment, growth, and osteogenesis were attributed to the nanosized surface topographies, roughness, and moderate wetting characteristics of the coatings. Their dimensional similarity to naturally occurring matrix proteins and crystals, coupled with their increased surface area for protein adsorption, may have facilitated the response. Therefore, this application of ultrafine powder coating technology affords highly biocompatible surfaces that can be readily modified to accentuate the Cellular response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2695–2709, 2012.
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mta enriched nanocomposite tio 2 polymeric powder coatings support human Mesenchymal Cell attachment and growth
Biomedical Materials, 2012Co-Authors: Wen Shi, Jesse Zhu, Hui Zhang, Mohammad Sayem Mozumder, Hiran PerinpanayagamAbstract:The objective of the study described in this paper was the development of novel polymer/ceramic nanocomposite coatings for implants through the application of ultrafine powder coating technology. Polyester resins were combined with µm-sized TiO(2) (25%) as the biocompatibility agent, nTiO(2) (0.5%) as the flow additive and mineral trioxide aggregates (ProRoot® MTA, 5%) as bioactive ceramics. Ultrafine powders were prepared and applied to titanium to create continuous polymeric powder coatings (PPCs) through the application of electrostatic ultrafine powder coating technology. Energy dispersive x-ray analysis confirmed that MTA had been incorporated into the PPCs, and elemental mapping showed that it had formed small clusters that were evenly distributed across the surface. Scanning electron microscopy (SEM) revealed continuous and smooth, but highly textured surface coatings that contrasted with the scalloped appearance of commercially pure titanium (cpTi) controls. Atomic force microscopy revealed intricate nano-topographies with an abundance of submicron-sized pits and nano-projections, evenly dispersed across their surfaces. Inverted fluorescence microscopy, SEM and Cell counts showed that human embryonic palatal Mesenchymal Cells attached and spread out onto PPC and MTA-enriched PPCs within 24 h. Mitochondrial enzyme activity measured viable and metabolically active Cells on all of the surfaces. After 72 h of growth, Cell counts and metabolic activity were significantly higher (P < 0.05) on the grey-MTA enriched PPC surfaces, than on unmodified PPC and cpTi. The novel polymer/ceramic nanocomposites that were created with ultrafine powder coating technology were continuous, homogenous and nano-rough coatings that enhanced human Mesenchymal Cell attachment and growth.
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nano tio 2 enriched polymeric powder coatings support human Mesenchymal Cell attachment and growth
Journal of Biomaterials Applications, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:The objective of this study was to utilize ultrafine powder coating technology to prepare (PPC) that can support human Mesenchymal Cell attachment and growth. Resins were modified with titanium dioxide and polytetrafluoroethylene (PTFE), and enriched with either SiO2 or TiO2 nanoparticles (nSiO2 or nTiO2) to create continuous PPC. Scanning electron microscopy (SEM) revealed complex surface topographies with nano features, and energy dispersive X-ray (EDX) analysis with Ti mapping confirmed a homogenous dispersion of the material. SEM and inverted fluorescence microscopy showed that human embryonic palatal Mesenchymal (HEPM) Cells attached and spread out on the PPC surfaces, particularly those enriched with nTiO 2. Cell counts were higher, and the MTT assay measured more metabolic activity from the nTiO2 enriched PPCs. Furthermore, these Cellular responses were enhanced on PPC surfaces that were enriched with a higher concentration of nTiO2 (2% vs. 0.5%), and appeared comparable to that seen on commerciall...
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TiO2-enriched polymeric powder coatings support human Mesenchymal Cell spreading and osteogenic differentiation
Biomedical Materials, 2011Co-Authors: Mohammad Sayem Mozumder, Jesse Zhu, Hiran PerinpanayagamAbstract:Novel polymeric powder coatings (PPC) were prepared by ultrafine powder coating technology and shown to support human Mesenchymal Cell attachment and growth. PPC surfaces enriched with nano-TiO2 (nTiO2) showed enhanced Cellular responses, and were compared to commercially pure titanium (cpTi). After Cell attachment and growth, osteogenic differentiation and bone matrix formation ensures osseointegration for implantable biomaterials. Therefore, the objective of this study was to determine if Mesenchymal Cells grown on PPC could undergo osteogenic differentiation by inducing Runx2 and bone matrix proteins, and then initiate mineralization. Atomic force microscopy revealed intricate three-dimensional micro-topographies, and the measures of nano-roughness and porosity were similar for all PPC surfaces. Scanning electron microscopy showed that the Cells attached and spread out over all of the surfaces. After 1 week in osteogenic media, RT-PCR analysis showed the induction of Runx2, the up-regulation of type I collagen, and the initial detection of alkaline phosphatase and bone sialoprotein. After 4 weeks, Alizarin Red staining showed mineral deposition. However, Cell spreading and osteogenic differentiation were significantly (P < 0.05) higher on the cpTi controls than on the PPC surfaces. Furthermore, spreading and differentiation were consistently higher on the titanium-enriched PPC-2, -3 and -4 than on the titanium-free PPC-1. Therefore, despite the presence of complex micro-topographies and nano-features, titanium-enrichment enhanced the Cellular response, and pure titanium still provided the best substrate. These findings confirm the cytocompatibility of these novel polymeric coatings and suggest that titanium-enrichment and nTiO2 additives may enhance their performance.