The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Marta Weinstock - One of the best experts on this subject based on the ideXlab platform.
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anti apoptotic action of anti alzheimer drug tv3326 n propargyl 3r aminoindan 5 yl ethyl Methyl Carbamate a novel cholinesterase monoamine oxidase inhibitor
Neuroscience Letters, 2003Co-Authors: Wakako Maruyama, Moussa B. H. Youdim, Marta Weinstock, Masayo Nagai, Makoto NaoiAbstract:The anti Parkinson drug, rasagiline [R-(+)-N-propargyl-1-aminoindan], an inhibitor of type B monoamine oxidase, has been shown to suppress apoptosis induced by neurotoxins and oxidative stress. A series of novel propargylaminoindans with a Carbamate moiety to inhibit cholinesterase were developed from phamacophore of rasagiline to protect or rescue deteriorated neurons in Alzheimer's and Lewy Body disease and provide a beneficial effect on the cognitive deficits. Rasagiline analogues were found to protect dopaminergic SH-SY5Y cells against apoptosis induced by peroxynitrite donor. SIN-1. TV3326, [(N-propargyl)-(3R)-aminoindan-5-yl]-ethyl Methyl Carbamate, was as effective as rasagiline in preventing apoptosis, followed by its S-enantiomer, TV3279. The anti-apoptotic-neuroprotective activity was shown to reside in the propargylamine and not the Carbamate moiety. This resulted in stabilization of the mitochondrial membrane potential, the collapse of which initiates the apoptotic cascade.
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Molecular Basis of Neuroprotective Activities of Rasagiline and the Anti-Alzheimer Drug TV3326 [lpar;N-Propargyl-(3R) Aminoindan-5-YL)-Ethyl Methyl Carbamate]
Cellular and Molecular Neurobiology, 2001Co-Authors: Moussa B. H. Youdim, Marta WeinstockAbstract:Rasagiline ( N -propargyl-1-( R )-aminoindan) is a selective, irreversible monoamine oxidase B (MAO B) inhibitor which has been developed as an anti-Parkinson drug. In controlled monotherapy and as adjunct to L-dopa it has shown anti-Parkinson activity. In cell culture (PC-12 and neuroblastoma SH-SY5Y cells) it exhibits neuroprotective and antiapoptotic activity against several neurotoxins (SIN-1, MPTP, 6-hydroxydopamine and N -Methyl-( R )-salsolinol) and ischemia. In vivo, it reduces the sequelae of traumatic brain injury in mice and speeds their recovery. The neuroprotective activity of rasagaline does not result from MAO B inhibition, since its S-enantiomer, TVP1022, which has 1000-fold weaker MAO inhibitory activity, exhibits similar neuroprotective properties. Introduction of a Carbamate moiety into the rasagiline molecule to confer cholinesterase inhibitory activity for the treatment of Alzheimer's disease, resulted in compounds TV3326 [( N -Propargyl-(3 R )Aminoindan-5-YL)-Ethyl Methyl Carbamate] and its S -enantiomer TV3279 [( N -Propargyl-(3 S ) Aminoindan-5-YL)-Ethyl Methyl Carbamate], which retain the neuroprotective activities of rasagiline and TVP1022. They also antagonize scopolamine-induced impairments in spatial memory. In addition, TV3326 exhibits brain-selective MAO A and B inhibitory activity after chronic administration and has antidepressant-like activity in the forced swim test. This is associated with an increase in brain levels of serotonin. The antiapoptotic activity of these propargylamine-containing derivatives may be related to their ability to delay the opening of voltage-dependent anion channels (VDAC), which are part of the mitochondrial permeability transition pore. The propargylamine moiety is responsible for the increase in the mitochondrial family of Bcl-2 proteins, prevention in the fall in mitochondrial membrane potential, prevention of the activation of caspase 3, and of translocation of glycerlaldehyde-3-phosphate dehydrogenase from the cytoplasm to the nucleus. The latter processes are closely associated with neurotoxin-induced apoptosis. Rasagiline interacts with and prevents the binding of PK11195 to the pro-apoptotic peripheral benzodiazepine receptor, which together with Bcl-2, hexokinase, porin, and adenine nucleotide translocator constitutes part of the VDAC. Furthermore, rasagiline, TV3326 and TV3279 are able to influence the processing of amyloid precursor protein by activation of alpha-secretase and increasing the release of soluble alpha APP in rat PC-12 and human neuroblastoma SH-SY5Y cells and in rat and mice cortex and hippocampus. This process has been shown to involve the upregulation of PKC and MAP kinase. It is quite likely that the induction of Bcl-2 and activation of PKC by rasagiline and TV3326 is closely linked to the anti-apototic action of these drugs and their ability to process APP by activation of alpha-secretase.
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molecular basis of neuroprotective activities of rasagiline and the anti alzheimer drug tv3326 n propargyl 3r aminoindan 5 yl ethyl Methyl Carbamate
Cellular and Molecular Neurobiology, 2001Co-Authors: Moussa B. H. Youdim, Marta WeinstockAbstract:Rasagiline (N-propargyl-1-(R)-aminoindan) is a selective, irreversible monoamine oxidase B (MAO B) inhibitor which has been developed as an anti-Parkinson drug. In controlled monotherapy and as adjunct to L-dopa it has shown anti-Parkinson activity. In cell culture (PC-12 and neuroblastoma SH-SY5Y cells) it exhibits neuroprotective and antiapoptotic activity against several neurotoxins (SIN-1, MPTP, 6-hydroxydopamine and N-Methyl-(R)-salsolinol) and ischemia. In vivo, it reduces the sequelae of traumatic brain injury in mice and speeds their recovery. The neuroprotective activity of rasagaline does not result from MAO B inhibition, since its S-enantiomer, TVP1022, which has 1000-fold weaker MAO inhibitory activity, exhibits similar neuroprotective properties. Introduction of a Carbamate moiety into the rasagiline molecule to confer cholinesterase inhibitory activity for the treatment of Alzheimer's disease, resulted in compounds TV3326 [(N-Propargyl-(3R)Aminoindan-5-YL)-Ethyl Methyl Carbamate] and its S-enantiomer TV3279 [(N-Propargyl-(3S) Aminoindan-5-YL)-Ethyl Methyl Carbamate], which retain the neuroprotective activities of rasagiline and TVP1022. They also antagonize scopolamine-induced impairments in spatial memory. In addition, TV3326 exhibits brain-selective MAO A and B inhibitory activity after chronic administration and has antidepressant-like activity in the forced swim test. This is associated with an increase in brain levels of serotonin. The antiapoptotic activity of these propargylamine-containing derivatives may be related to their ability to delay the opening of voltage-dependent anion channels (VDAC), which are part of the mitochondrial permeability transition pore. The propargylamine moiety is responsible for the increase in the mitochondrial family of Bcl-2 proteins, prevention in the fall in mitochondrial membrane potential, prevention of the activation of caspase 3, and of translocation of glycerlaldehyde-3-phosphate dehydrogenase from the cytoplasm to the nucleus. The latter processes are closely associated with neurotoxin-induced apoptosis. Rasagiline interacts with and prevents the binding of PK11195 to the pro-apoptotic peripheral benzodiazepine receptor, which together with Bcl-2, hexokinase, porin, and adenine nucleotide translocator constitutes part of the VDAC. Furthermore, rasagiline, TV3326 and TV3279 are able to influence the processing of amyloid precursor protein by activation of alpha-secretase and increasing the release of soluble alpha APP in rat PC-12 and human neuroblastoma SH-SY5Y cells and in rat and mice cortex and hippocampus. This process has been shown to involve the upregulation of PKC and MAP kinase. It is quite likely that the induction of Bcl-2 and activation of PKC by rasagiline and TV3326 is closely linked to the anti-apototic action of these drugs and their ability to process APP by activation of alpha-secretase.
Hisamitsu Nagase - One of the best experts on this subject based on the ideXlab platform.
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High Throughput Analysis of N‐Methyl Carbamate Pesticides in Cereals and Beans by Dual Countercurrent Chromatography and Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry
Journal of Liquid Chromatography & Related Technologies, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu Nagase, Yoichiro ItoAbstract:Abstract We developed a new analytical method for analysis of N‐Methyl Carbamate pesticides in cereals and beans using dual counter‐current chromatography (dual CCC) and liquid chromatography‐electrospray ionization tandem mass spectrometry (ESI LC/MS/MS). After pesticides were extracted from cereals and beans with ethyl acetate, each extract was cleaned up by dual CCC using a non‐aqueous binary solvent system composed of n‐hexane‐acetonitrile and analyzed by ESI LC/MS/MS with a short column. The average recoveries from cereals and beans fortified at the level of 0.01 ppm ranged from 73.9 to 119.6%, with the coefficients of variation from 0.7 to 6.8%. At the fortified level of 0.5 ppm, the recoveries ranged from 72.1 to 117.1% with coefficients of variation from 0.4 to 9.3%. The present analytical method of N‐Methyl Carbamate pesticides in cereals and beans is considered to be useful for monitoring the pesticide residues in cereals and beans.
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high throughput analysis of n Methyl Carbamate pesticides in cereals and beans by dual countercurrent chromatography and liquid chromatography electrospray ionization tandem mass spectrometry
Journal of Liquid Chromatography & Related Technologies, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu Nagase, Yoichiro ItoAbstract:Abstract We developed a new analytical method for analysis of N‐Methyl Carbamate pesticides in cereals and beans using dual counter‐current chromatography (dual CCC) and liquid chromatography‐electrospray ionization tandem mass spectrometry (ESI LC/MS/MS). After pesticides were extracted from cereals and beans with ethyl acetate, each extract was cleaned up by dual CCC using a non‐aqueous binary solvent system composed of n‐hexane‐acetonitrile and analyzed by ESI LC/MS/MS with a short column. The average recoveries from cereals and beans fortified at the level of 0.01 ppm ranged from 73.9 to 119.6%, with the coefficients of variation from 0.7 to 6.8%. At the fortified level of 0.5 ppm, the recoveries ranged from 72.1 to 117.1% with coefficients of variation from 0.4 to 9.3%. The present analytical method of N‐Methyl Carbamate pesticides in cereals and beans is considered to be useful for monitoring the pesticide residues in cereals and beans.
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the high throughput analysis of n Methyl Carbamate pesticides in fruits and vegetables by liquid chromatography electrospray ionization tandem mass spectrometry using a short column
Analytica Chimica Acta, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu NagaseAbstract:Abstract We developed a new analysis method for the nine N -Methyl Carbamate pesticides in fruits and vegetables using ESI LC/MS/MS with direct sample injection into a short column. After extraction of the pesticides with ethyl acetate from sample, the extract is evaporated to dryness and redissolved in ultra pure water before injection into LC/MS/MS. The method needs no cleanup steps. The average recoveries from fruits and vegetables fortified at the level of 0.01 μg/g ranged from 56.0 to 119.1% with the coefficients of variation ranging from 0.2 to 7.6% for intra-day ( n = 5 × 3 days) and from 0.8 to 18.4% for inter-day ( n = 15). At the fortified level of 0.5 μg/g, the recoveries ranged from 67.7 to 119.3% with the coefficients of variation ranging from 0.5 to 7.8% for intra-day ( n = 5 × 3 days) and from 0.9 to 14.8% for inter-day ( n = 15). The method is considered to be satisfactory for the monitoring of the Carbamate pesticide residues in fruits and vegetables, suggesting that the present method is applicable to other pesticide residues in foods.
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the high throughput analysis of n Methyl Carbamate pesticides in wine and juice by electrospray ionization liquid chromatography tandem mass spectrometry with direct sample injection into a short column
Analytica Chimica Acta, 2005Co-Authors: Tomomi Goto, Yuko Ito, Hiroshi Matsumoto, Hisao Oka, Isao Saito, Hideo Sugiyama, Chiyoji Ohkubo, Hiroyuki Nakazawa, Hisamitsu NagaseAbstract:Abstract We developed a new analysis method for the N -Methyl Carbamate pesticides in juice and wine. The juice and wine were diluted with ultra pure water, and determined by electrospray ionization tandem mass spectrometry (ESI LC/MS/MS) with direct sample injection into a short column. The new method, including sample preparation and determination, is simple and rapid, and allows simultaneous determination of nine N -Methyl Carbamate pesticides in juice and wine within analysis time that is much shorter as compared with the traditional method. The average recoveries from juice and wine fortified at the level of 0.1 ppm ranged from 59.6 to 126.7% with the coefficients of variation ranging from 0.4 to 5.1% for intra-day ( n = 5 × 3 days) and from 0.5 to 22.6% for inter-day ( n = 15). At the fortified level of 0.5 ppm, the recoveries ranged from 69.3 to 127.2% with the coefficients of variation ranging from 0.4 to 6.9% for intra-day ( n = 5 × 3 days) and from 0.5 to 22.6% for inter-day ( n = 15). The method is considered to be satisfactory for the monitoring of the Carbamate pesticides residues in juice and wine, suggesting that the present method is applicable to other pesticide residues in foods.
Juha P. Heiskanen - One of the best experts on this subject based on the ideXlab platform.
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synthesis of alkaline soluble cellulose Methyl Carbamate using a reactive deep eutectic solvent
Chemsuschem, 2017Co-Authors: Juho Antti Sirviö, Juha P. HeiskanenAbstract:This study presents the use of a reactive deep eutectic solvent (DES) for the chemical modification of wood cellulose fibers. DES based on diMethylurea and ZnCl2 was used to synthetize cellulose Methyl Carbamate (CMeC). This synthesis was performed at elevated temperature under solvent-free conditions. Chemical characterization based on FTIR and NMR indicated that Methyl Carbamate was successfully introduced to cellulose, and a degree of substitution (DS) of 0.17 was obtained after 3 h of reaction at 150 °C. The product with a DS of 0.17 exhibited good alkaline solubility (in 3 % NaOH solution) after freeze-thawing, whereas the original cellulose fibers were practically insoluble even in 9 % NaOH. As diMethylurea can be produced from CO2, this method can be used as a sustainable way to obtain novel cellulose materials with desirable properties for use in a wide range of applications.
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Synthesis of Alkaline‐Soluble Cellulose Methyl Carbamate Using a Reactive Deep Eutectic Solvent
ChemSusChem, 2016Co-Authors: Juho Antti Sirviö, Juha P. HeiskanenAbstract:This study presents the use of a reactive deep eutectic solvent (DES) for the chemical modification of wood cellulose fibers. DES based on diMethylurea and ZnCl2 was used to synthetize cellulose Methyl Carbamate (CMeC). This synthesis was performed at elevated temperature under solvent-free conditions. Chemical characterization based on FTIR and NMR indicated that Methyl Carbamate was successfully introduced to cellulose, and a degree of substitution (DS) of 0.17 was obtained after 3 h of reaction at 150 °C. The product with a DS of 0.17 exhibited good alkaline solubility (in 3 % NaOH solution) after freeze-thawing, whereas the original cellulose fibers were practically insoluble even in 9 % NaOH. As diMethylurea can be produced from CO2, this method can be used as a sustainable way to obtain novel cellulose materials with desirable properties for use in a wide range of applications.
Moussa B. H. Youdim - One of the best experts on this subject based on the ideXlab platform.
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anti apoptotic action of anti alzheimer drug tv3326 n propargyl 3r aminoindan 5 yl ethyl Methyl Carbamate a novel cholinesterase monoamine oxidase inhibitor
Neuroscience Letters, 2003Co-Authors: Wakako Maruyama, Moussa B. H. Youdim, Marta Weinstock, Masayo Nagai, Makoto NaoiAbstract:The anti Parkinson drug, rasagiline [R-(+)-N-propargyl-1-aminoindan], an inhibitor of type B monoamine oxidase, has been shown to suppress apoptosis induced by neurotoxins and oxidative stress. A series of novel propargylaminoindans with a Carbamate moiety to inhibit cholinesterase were developed from phamacophore of rasagiline to protect or rescue deteriorated neurons in Alzheimer's and Lewy Body disease and provide a beneficial effect on the cognitive deficits. Rasagiline analogues were found to protect dopaminergic SH-SY5Y cells against apoptosis induced by peroxynitrite donor. SIN-1. TV3326, [(N-propargyl)-(3R)-aminoindan-5-yl]-ethyl Methyl Carbamate, was as effective as rasagiline in preventing apoptosis, followed by its S-enantiomer, TV3279. The anti-apoptotic-neuroprotective activity was shown to reside in the propargylamine and not the Carbamate moiety. This resulted in stabilization of the mitochondrial membrane potential, the collapse of which initiates the apoptotic cascade.
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Molecular Basis of Neuroprotective Activities of Rasagiline and the Anti-Alzheimer Drug TV3326 [lpar;N-Propargyl-(3R) Aminoindan-5-YL)-Ethyl Methyl Carbamate]
Cellular and Molecular Neurobiology, 2001Co-Authors: Moussa B. H. Youdim, Marta WeinstockAbstract:Rasagiline ( N -propargyl-1-( R )-aminoindan) is a selective, irreversible monoamine oxidase B (MAO B) inhibitor which has been developed as an anti-Parkinson drug. In controlled monotherapy and as adjunct to L-dopa it has shown anti-Parkinson activity. In cell culture (PC-12 and neuroblastoma SH-SY5Y cells) it exhibits neuroprotective and antiapoptotic activity against several neurotoxins (SIN-1, MPTP, 6-hydroxydopamine and N -Methyl-( R )-salsolinol) and ischemia. In vivo, it reduces the sequelae of traumatic brain injury in mice and speeds their recovery. The neuroprotective activity of rasagaline does not result from MAO B inhibition, since its S-enantiomer, TVP1022, which has 1000-fold weaker MAO inhibitory activity, exhibits similar neuroprotective properties. Introduction of a Carbamate moiety into the rasagiline molecule to confer cholinesterase inhibitory activity for the treatment of Alzheimer's disease, resulted in compounds TV3326 [( N -Propargyl-(3 R )Aminoindan-5-YL)-Ethyl Methyl Carbamate] and its S -enantiomer TV3279 [( N -Propargyl-(3 S ) Aminoindan-5-YL)-Ethyl Methyl Carbamate], which retain the neuroprotective activities of rasagiline and TVP1022. They also antagonize scopolamine-induced impairments in spatial memory. In addition, TV3326 exhibits brain-selective MAO A and B inhibitory activity after chronic administration and has antidepressant-like activity in the forced swim test. This is associated with an increase in brain levels of serotonin. The antiapoptotic activity of these propargylamine-containing derivatives may be related to their ability to delay the opening of voltage-dependent anion channels (VDAC), which are part of the mitochondrial permeability transition pore. The propargylamine moiety is responsible for the increase in the mitochondrial family of Bcl-2 proteins, prevention in the fall in mitochondrial membrane potential, prevention of the activation of caspase 3, and of translocation of glycerlaldehyde-3-phosphate dehydrogenase from the cytoplasm to the nucleus. The latter processes are closely associated with neurotoxin-induced apoptosis. Rasagiline interacts with and prevents the binding of PK11195 to the pro-apoptotic peripheral benzodiazepine receptor, which together with Bcl-2, hexokinase, porin, and adenine nucleotide translocator constitutes part of the VDAC. Furthermore, rasagiline, TV3326 and TV3279 are able to influence the processing of amyloid precursor protein by activation of alpha-secretase and increasing the release of soluble alpha APP in rat PC-12 and human neuroblastoma SH-SY5Y cells and in rat and mice cortex and hippocampus. This process has been shown to involve the upregulation of PKC and MAP kinase. It is quite likely that the induction of Bcl-2 and activation of PKC by rasagiline and TV3326 is closely linked to the anti-apototic action of these drugs and their ability to process APP by activation of alpha-secretase.
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molecular basis of neuroprotective activities of rasagiline and the anti alzheimer drug tv3326 n propargyl 3r aminoindan 5 yl ethyl Methyl Carbamate
Cellular and Molecular Neurobiology, 2001Co-Authors: Moussa B. H. Youdim, Marta WeinstockAbstract:Rasagiline (N-propargyl-1-(R)-aminoindan) is a selective, irreversible monoamine oxidase B (MAO B) inhibitor which has been developed as an anti-Parkinson drug. In controlled monotherapy and as adjunct to L-dopa it has shown anti-Parkinson activity. In cell culture (PC-12 and neuroblastoma SH-SY5Y cells) it exhibits neuroprotective and antiapoptotic activity against several neurotoxins (SIN-1, MPTP, 6-hydroxydopamine and N-Methyl-(R)-salsolinol) and ischemia. In vivo, it reduces the sequelae of traumatic brain injury in mice and speeds their recovery. The neuroprotective activity of rasagaline does not result from MAO B inhibition, since its S-enantiomer, TVP1022, which has 1000-fold weaker MAO inhibitory activity, exhibits similar neuroprotective properties. Introduction of a Carbamate moiety into the rasagiline molecule to confer cholinesterase inhibitory activity for the treatment of Alzheimer's disease, resulted in compounds TV3326 [(N-Propargyl-(3R)Aminoindan-5-YL)-Ethyl Methyl Carbamate] and its S-enantiomer TV3279 [(N-Propargyl-(3S) Aminoindan-5-YL)-Ethyl Methyl Carbamate], which retain the neuroprotective activities of rasagiline and TVP1022. They also antagonize scopolamine-induced impairments in spatial memory. In addition, TV3326 exhibits brain-selective MAO A and B inhibitory activity after chronic administration and has antidepressant-like activity in the forced swim test. This is associated with an increase in brain levels of serotonin. The antiapoptotic activity of these propargylamine-containing derivatives may be related to their ability to delay the opening of voltage-dependent anion channels (VDAC), which are part of the mitochondrial permeability transition pore. The propargylamine moiety is responsible for the increase in the mitochondrial family of Bcl-2 proteins, prevention in the fall in mitochondrial membrane potential, prevention of the activation of caspase 3, and of translocation of glycerlaldehyde-3-phosphate dehydrogenase from the cytoplasm to the nucleus. The latter processes are closely associated with neurotoxin-induced apoptosis. Rasagiline interacts with and prevents the binding of PK11195 to the pro-apoptotic peripheral benzodiazepine receptor, which together with Bcl-2, hexokinase, porin, and adenine nucleotide translocator constitutes part of the VDAC. Furthermore, rasagiline, TV3326 and TV3279 are able to influence the processing of amyloid precursor protein by activation of alpha-secretase and increasing the release of soluble alpha APP in rat PC-12 and human neuroblastoma SH-SY5Y cells and in rat and mice cortex and hippocampus. This process has been shown to involve the upregulation of PKC and MAP kinase. It is quite likely that the induction of Bcl-2 and activation of PKC by rasagiline and TV3326 is closely linked to the anti-apototic action of these drugs and their ability to process APP by activation of alpha-secretase.
Tomomi Goto - One of the best experts on this subject based on the ideXlab platform.
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Development of Analytical Methods for Residual N‐Methyl Carbamate Pesticides in Foods
ChemInform, 2010Co-Authors: Tomomi GotoAbstract:One of the major roles of public health agencies is to ensure safe products for consumers through analysis of residual agricultural chemicals and veterinary drugs in foods. The use of agricultural chemicals and veterinary drugs in agriculture is necessary to improve the quality of the food produced. They play a beneficial role in providing a plentiful, low-cost supply of high-quality food. On the other hand, as a consequence of this use, the presence of residues in food that is a critical element of overall public health is unavoidable, and residues in food are of great importance in the evaluation of food quality. N-Methyl Carbamate pesticides have anticholinesterase activity and are used worldwide. The postcolumn HPLC method is widely used for the detection of N-Methyl Carbamate pesticides in food. However, this traditional method involves a time-consuming process that requires skillful techniques. Therefore we developed a new analysis method for N-Methyl Carbamate pesticides as described in detail in this paper. The new method, including sample preparation and determination, is simple and rapid and allows simultaneous determination of pesticides in food within a much shorter analysis time as compared with the traditional method. This should provide high-quality analysis and ensure safe products for consumers.
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Development of analytical methods for residual N-Methyl Carbamate pesticides in foods
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 2010Co-Authors: Tomomi GotoAbstract:One of the major roles of public health agencies is to ensure safe products for consumers through analysis of residual agricultural chemicals and veterinary drugs in foods. The use of agricultural chemicals and veterinary drugs in agriculture is necessary to improve the quality of the food produced. They play a beneficial role in providing a plentiful, low-cost supply of high-quality food. On the other hand, as a consequence of this use, the presence of residues in food that is a critical element of overall public health is unavoidable, and residues in food are of great importance in the evaluation of food quality. N-Methyl Carbamate pesticides have anticholinesterase activity and are used worldwide. The postcolumn HPLC method is widely used for the detection of N-Methyl Carbamate pesticides in food. However, this traditional method involves a time-consuming process that requires skillful techniques. Therefore we developed a new analysis method for N-Methyl Carbamate pesticides as described in detail in this paper. The new method, including sample preparation and determination, is simple and rapid and allows simultaneous determination of pesticides in food within a much shorter analysis time as compared with the traditional method. This should provide high-quality analysis and ensure safe products for consumers.
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High Throughput Analysis of N‐Methyl Carbamate Pesticides in Cereals and Beans by Dual Countercurrent Chromatography and Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry
Journal of Liquid Chromatography & Related Technologies, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu Nagase, Yoichiro ItoAbstract:Abstract We developed a new analytical method for analysis of N‐Methyl Carbamate pesticides in cereals and beans using dual counter‐current chromatography (dual CCC) and liquid chromatography‐electrospray ionization tandem mass spectrometry (ESI LC/MS/MS). After pesticides were extracted from cereals and beans with ethyl acetate, each extract was cleaned up by dual CCC using a non‐aqueous binary solvent system composed of n‐hexane‐acetonitrile and analyzed by ESI LC/MS/MS with a short column. The average recoveries from cereals and beans fortified at the level of 0.01 ppm ranged from 73.9 to 119.6%, with the coefficients of variation from 0.7 to 6.8%. At the fortified level of 0.5 ppm, the recoveries ranged from 72.1 to 117.1% with coefficients of variation from 0.4 to 9.3%. The present analytical method of N‐Methyl Carbamate pesticides in cereals and beans is considered to be useful for monitoring the pesticide residues in cereals and beans.
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high throughput analysis of n Methyl Carbamate pesticides in cereals and beans by dual countercurrent chromatography and liquid chromatography electrospray ionization tandem mass spectrometry
Journal of Liquid Chromatography & Related Technologies, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu Nagase, Yoichiro ItoAbstract:Abstract We developed a new analytical method for analysis of N‐Methyl Carbamate pesticides in cereals and beans using dual counter‐current chromatography (dual CCC) and liquid chromatography‐electrospray ionization tandem mass spectrometry (ESI LC/MS/MS). After pesticides were extracted from cereals and beans with ethyl acetate, each extract was cleaned up by dual CCC using a non‐aqueous binary solvent system composed of n‐hexane‐acetonitrile and analyzed by ESI LC/MS/MS with a short column. The average recoveries from cereals and beans fortified at the level of 0.01 ppm ranged from 73.9 to 119.6%, with the coefficients of variation from 0.7 to 6.8%. At the fortified level of 0.5 ppm, the recoveries ranged from 72.1 to 117.1% with coefficients of variation from 0.4 to 9.3%. The present analytical method of N‐Methyl Carbamate pesticides in cereals and beans is considered to be useful for monitoring the pesticide residues in cereals and beans.
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the high throughput analysis of n Methyl Carbamate pesticides in fruits and vegetables by liquid chromatography electrospray ionization tandem mass spectrometry using a short column
Analytica Chimica Acta, 2006Co-Authors: Tomomi Goto, Yuko Ito, Sadaji Yamada, Hiroshi Matsumoto, Hisao Oka, Hisamitsu NagaseAbstract:Abstract We developed a new analysis method for the nine N -Methyl Carbamate pesticides in fruits and vegetables using ESI LC/MS/MS with direct sample injection into a short column. After extraction of the pesticides with ethyl acetate from sample, the extract is evaporated to dryness and redissolved in ultra pure water before injection into LC/MS/MS. The method needs no cleanup steps. The average recoveries from fruits and vegetables fortified at the level of 0.01 μg/g ranged from 56.0 to 119.1% with the coefficients of variation ranging from 0.2 to 7.6% for intra-day ( n = 5 × 3 days) and from 0.8 to 18.4% for inter-day ( n = 15). At the fortified level of 0.5 μg/g, the recoveries ranged from 67.7 to 119.3% with the coefficients of variation ranging from 0.5 to 7.8% for intra-day ( n = 5 × 3 days) and from 0.9 to 14.8% for inter-day ( n = 15). The method is considered to be satisfactory for the monitoring of the Carbamate pesticide residues in fruits and vegetables, suggesting that the present method is applicable to other pesticide residues in foods.