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Ivonne M.c.m. Rietjens - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Interindividual Human Variation in Bioactivation and DNA Adduct Formation of Estragole in Liver Predicted by Physiologically Based Kinetic/Dynamic and Monte Carlo Modeling
    Chemical research in toxicology, 2016
    Co-Authors: Ans Punt, Benoît Schilter, Peter J. Van Bladeren, Alicia Paini, A. Spenkelink, Gabriele Scholz, Ivonne M.c.m. Rietjens
    Abstract:

    Estragole is a known hepatocarcinogen in rodents at high doses following metabolic conversion to the DNA-reactive metabolite 1'-sulfooxyestragole. The aim of the present study was to model possible levels of DNA adduct formation in (individual) humans upon exposure to estragole. This was done by extending a previously defined PBK model for estragole in humans to include (i) new data on interindividual variation in the kinetics for the major PBK model parameters influencing the formation of 1'-sulfooxyestragole, (ii) an equation describing the relationship between 1'-sulfooxyestragole and DNA adduct formation, (iii) Monte Carlo modeling to simulate interindividual human variation in DNA adduct formation in the population, and (iv) a comparison of the predictions made to human data on DNA adduct formation for the related alkenylbenzene Methyleugenol. Adequate model predictions could be made, with the predicted DNA adduct levels at the estimated daily intake of estragole of 0.01 mg/kg bw ranging between 1.6 and 8.8 adducts in 10(8) nucleotides (nts) (50th and 99th percentiles, respectively). This is somewhat lower than values reported in the literature for the related alkenylbenzene Methyleugenol in surgical human liver samples. The predicted levels seem to be below DNA adduct levels that are linked with tumor formation by alkenylbenzenes in rodents, which were estimated to amount to 188-500 adducts per 10(8) nts at the BMD10 values of estragole and Methyleugenol. Although this does not seem to point to a significant health concern for human dietary exposure, drawing firm conclusions may have to await further validation of the model's predictions.

  • Evaluation of Interindividual Human Variation in Bioactivation and DNA Adduct Formation of Estragole in Liver Predicted by Physiologically Based Kinetic/Dynamic and Monte Carlo Modeling
    2016
    Co-Authors: Ans Punt, Benoît Schilter, Peter J. Van Bladeren, Alicia Paini, A. Spenkelink, Gabriele Scholz, Ivonne M.c.m. Rietjens
    Abstract:

    Estragole is a known hepatocarcinogen in rodents at high doses following metabolic conversion to the DNA-reactive metabolite 1′-sulfooxyestragole. The aim of the present study was to model possible levels of DNA adduct formation in (individual) humans upon exposure to estragole. This was done by extending a previously defined PBK model for estragole in humans to include (i) new data on interindividual variation in the kinetics for the major PBK model parameters influencing the formation of 1′-sulfooxyestragole, (ii) an equation describing the relationship between 1′-sulfooxyestragole and DNA adduct formation, (iii) Monte Carlo modeling to simulate interindividual human variation in DNA adduct formation in the population, and (iv) a comparison of the predictions made to human data on DNA adduct formation for the related alkenylbenzene Methyleugenol. Adequate model predictions could be made, with the predicted DNA adduct levels at the estimated daily intake of estragole of 0.01 mg/kg bw ranging between 1.6 and 8.8 adducts in 108 nucleotides (nts) (50th and 99th percentiles, respectively). This is somewhat lower than values reported in the literature for the related alkenylbenzene Methyleugenol in surgical human liver samples. The predicted levels seem to be below DNA adduct levels that are linked with tumor formation by alkenylbenzenes in rodents, which were estimated to amount to 188–500 adducts per 108 nts at the BMD10 values of estragole and Methyleugenol. Although this does not seem to point to a significant health concern for human dietary exposure, drawing firm conclusions may have to await further validation of the model’s predictions

  • Physiologically based kinetic models for the alkenylbenzene elemicin in rat and human and possible implications for risk assessment.
    Chemical research in toxicology, 2012
    Co-Authors: Suzanne J. P. L. Van Den Berg, Jacques Vervoort, Ans Punt, Ans E. M. F. Soffers, Stephen Ngeleja, Bert Spenkelink, Ivonne M.c.m. Rietjens
    Abstract:

    The present study describes physiologically based kinetic (PBK) models for the alkenylbenzene elemicin (3,4,5-trimethoxyallylbenzene) in rat and human, based on the PBK models previously developed for the structurally related alkenylbenzenes estragole, Methyleugenol, and safrole. Using the newly developed models, the level of metabolic activation of elemicin in rat and human was predicted to obtain insight in species differences in the bioactivation of elemicin and read across to the other methoxy allylbenzenes, estragole and Methyleugenol. Results reveal that the differences between rat and human in the formation of the proximate carcinogenic metabolite 1'-hydroxyelemicin and the ultimate carcinogenic metabolite 1'-sulfoxyelemicin are limited (

  • Physiologically Based Kinetic Models for the Alkenylbenzene Elemicin in Rat and Human and Possible Implications for Risk Assessment
    2012
    Co-Authors: Suzanne J. P. L. Van Den Berg, Jacques Vervoort, Ans Punt, Ans E. M. F. Soffers, Stephen Ngeleja, Bert Spenkelink, Ivonne M.c.m. Rietjens
    Abstract:

    The present study describes physiologically based kinetic (PBK) models for the alkenylbenzene elemicin (3,4,5-trimethoxyallylbenzene) in rat and human, based on the PBK models previously developed for the structurally related alkenylbenzenes estragole, Methyleugenol, and safrole. Using the newly developed models, the level of metabolic activation of elemicin in rat and human was predicted to obtain insight in species differences in the bioactivation of elemicin and read across to the other methoxy allylbenzenes, estragole and Methyleugenol. Results reveal that the differences between rat and human in the formation of the proximate carcinogenic metabolite 1′-hydroxyelemicin and the ultimate carcinogenic metabolite 1′-sulfoxyelemicin are limited (

Youngjoon Ahn - One of the best experts on this subject based on the ideXlab platform.

  • Fumigant Toxicity of Phenylpropanoids Identified in Asarum sieboldii Aerial Parts to Lycoriella ingenua (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae)
    Journal of Economic Entomology, 2015
    Co-Authors: Haribalan Perumalsamy, Karuppasamy Sankarapandian, Byeoung-ryeol Choi, Youngjoon Ahn
    Abstract:

    ABSTRACT Lycoriella ingenua (Dufour) (Diptera: Sciaridae) and Coboldia fuscipes (Meigen) (Diptera: Scatopsidae) are two of the most economically important insect pests of cultivated mushrooms. The toxicities to the fly larvae of the three phenylpropanoids (Methyleugenol, myristicin, and safrole) from aerial parts of Asarum sieboldii Miquel (Aristolochiaceae) were compared with those of the currently available carbamate insecticide benfuracarb. In a contactþfumigant mortality bioassay with L. ingenua and C. fuscipes larvae, Methyleugenol (1.46 and 2.33 µg/cm2) was the most toxic compound, followed by safrole (2.03 and 2.59 µg/cm2) and myristicin (3.59 and 4.96 µg/cm2), based on 24-h LC50 values. The phenylpropanoids were less toxic than benfuracarb (LC50, 0.75 and 0.55 µg/cm2). In vaporphase mortality tests with the larvae, the phenylpropanoids were consistently more toxic in closed versus open containers, indicating that the effect of the compounds was largely a result of vapor action. Global efforts to r...

  • larvicidal activity of asarum heterotropoides root constituents against insecticide susceptible and resistant culex pipiens pallens and aedes aegypti and ochlerotatus togoi
    Journal of Agricultural and Food Chemistry, 2010
    Co-Authors: Haribalan Perumalsamy, Kyu Sik Chang, Chan Park, Youngjoon Ahn
    Abstract:

    We investigated the toxicity of (-)-asarinin, α-asarone, Methyleugenol, pellitorine, and pentadecane identified in Asarum heterotropoides root to third instar larvae from insecticide-susceptible Culex pipiens pallens (KS-CP strain), Aedes aegypti, and Ochlerotatus togoi as well as field-collected C. p. pallens (DJ-CP colony), identified by polymerase chain reaction. Results were compared with those of two conventional mosquito larvicides: fenthion and temephos. Pellitorine (LC50, 2.08, 2.33, and 2.38 ppm) was 5.5, 10.8, and 25.6 times, 4.5, 11.6, and 24.7 times, and 6.9, 11.1, and 24.6 times more toxic than (-)-asarinin, α-asarone, and Methyleugenol against susceptible C. p. pallens, A. aegypti, and O. togoi larvae, respectively. Pentadecane was least toxic. Overall, all the compounds were less toxic than either fenthion or temephos. However, these compounds did not differ in toxicity against larvae from the two Culex strains, even though the DJ-CP larvae exhibited high levels of resistance to fenthion (resistance ratio (RR), 1179), chlorpyrifos (RR, 1174), fenitrothion (RR, 428), deltamethrin (RR, 316), chlorfenapyr (RR, 225), and α-cypermethrin (RR, 94). This finding indicates that the isolated compounds and the pyrethroid, organophosphorus, and pyrrole insecticides do not share a common mode of action or elicit cross-resistance. A. heterotropoides root-derived materials, particularly (-)-asarinin and pellitorine, merit further study as potential mosquito larvicides for the control of insecticide-resistant mosquito populations in light of global efforts to reduce the level of highly toxic synthetic insecticides in the aquatic environment.

  • acaricidal activity of clove bud oil compounds against dermatophagoides farinae and dermatophagoides pteronyssinus acari pyroglyphidae
    Journal of Agricultural and Food Chemistry, 2003
    Co-Authors: Eunhee Kim, Hyun Kyung Kim, Youngjoon Ahn
    Abstract:

    The acaricidal activity of clove (Eugenia caryophyllata) bud oil-derived eugenol and its congeners (acetyleugenol, isoeugenol, and Methyleugenol) against adults of Dermatophagoides farinae and Dermatophagoides pteronyssinus was examined using direct contact application and fumigation methods and compared with those of benzyl benzoate and N,N-diethyl-m-toluamide (DEET). Responses varied according to compound, dose, and mite species. On the basis of LD50 values, the compound most toxic to D. farinae adults was Methyleugenol (0.94 μg/cm2) followed by isoeugenol (5.17 μg/cm2), eugenol (5.47 μg/cm2), benzyl benzoate (9.22 μg/cm2), and acetyleugenol (14.16 μg/cm2). Very low activity was observed with DEET (37.59 μg/cm2). Against D. pteronyssinus adults, Methyleugenol (0.67 μg/cm2) was much more effective than isoeugenol (1.55 μg/cm2), eugenol (3.71 μg/cm2), acetyleugenol (5.41 μg/cm2), and benzyl benzoate (6.59 μg/cm2). DEET (17.85 μg/cm2) was least toxic. These results indicate that the lipophilicity of the fo...

  • acaricidal activity of clove bud oil compounds against dermatophagoides farinae and dermatophagoides pteronyssinus acari pyroglyphidae
    Journal of Agricultural and Food Chemistry, 2003
    Co-Authors: Eunhee Kim, Hyun Kyung Kim, Youngjoon Ahn
    Abstract:

    The acaricidal activity of clove (Eugenia caryophyllata) bud oil-derived eugenol and its congeners (acetyleugenol, isoeugenol, and Methyleugenol) against adults of Dermatophagoides farinae and Dermatophagoides pteronyssinus was examined using direct contact application and fumigation methods and compared with those of benzyl benzoate and N,N-diethyl-m-toluamide (DEET). Responses varied according to compound, dose, and mite species. On the basis of LD(50) values, the compound most toxic to D. farinae adults was Methyleugenol (0.94 microg/cm(2)) followed by isoeugenol (5.17 microg/cm(2)), eugenol (5.47 microg/cm(2)), benzyl benzoate (9.22 microg/cm(2)), and acetyleugenol (14.16 microg/cm(2)). Very low activity was observed with DEET (37.59 microg/cm(2)). Against D. pteronyssinus adults, Methyleugenol (0.67 microg/cm(2)) was much more effective than isoeugenol (1.55 microg/cm(2)), eugenol (3.71 microg/cm(2)), acetyleugenol (5.41 microg/cm(2)), and benzyl benzoate (6.59 microg/cm(2)). DEET (17.85 microg/cm(2)) was least toxic. These results indicate that the lipophilicity of the four phenylpropenes plays a crucial role in dust mite toxicity. The typical poisoning symptom of eugenol and its congeners was a similar death symptom of the forelegs extended forward together, leading to death without knockdown, whereas benzyl benzoate and DEET caused death following uncoordinated behavior. In a fumigation test with both mite species, all four phenylpropenes were much more effective in closed containers than in open ones, indicating that the mode of delivery of these compounds was largely due to action in the vapor phase. Eugenol and its congeners merit further study as potential house dust mite control agents or as lead compounds.

Ans Punt - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Interindividual Human Variation in Bioactivation and DNA Adduct Formation of Estragole in Liver Predicted by Physiologically Based Kinetic/Dynamic and Monte Carlo Modeling
    Chemical research in toxicology, 2016
    Co-Authors: Ans Punt, Benoît Schilter, Peter J. Van Bladeren, Alicia Paini, A. Spenkelink, Gabriele Scholz, Ivonne M.c.m. Rietjens
    Abstract:

    Estragole is a known hepatocarcinogen in rodents at high doses following metabolic conversion to the DNA-reactive metabolite 1'-sulfooxyestragole. The aim of the present study was to model possible levels of DNA adduct formation in (individual) humans upon exposure to estragole. This was done by extending a previously defined PBK model for estragole in humans to include (i) new data on interindividual variation in the kinetics for the major PBK model parameters influencing the formation of 1'-sulfooxyestragole, (ii) an equation describing the relationship between 1'-sulfooxyestragole and DNA adduct formation, (iii) Monte Carlo modeling to simulate interindividual human variation in DNA adduct formation in the population, and (iv) a comparison of the predictions made to human data on DNA adduct formation for the related alkenylbenzene Methyleugenol. Adequate model predictions could be made, with the predicted DNA adduct levels at the estimated daily intake of estragole of 0.01 mg/kg bw ranging between 1.6 and 8.8 adducts in 10(8) nucleotides (nts) (50th and 99th percentiles, respectively). This is somewhat lower than values reported in the literature for the related alkenylbenzene Methyleugenol in surgical human liver samples. The predicted levels seem to be below DNA adduct levels that are linked with tumor formation by alkenylbenzenes in rodents, which were estimated to amount to 188-500 adducts per 10(8) nts at the BMD10 values of estragole and Methyleugenol. Although this does not seem to point to a significant health concern for human dietary exposure, drawing firm conclusions may have to await further validation of the model's predictions.

  • Evaluation of Interindividual Human Variation in Bioactivation and DNA Adduct Formation of Estragole in Liver Predicted by Physiologically Based Kinetic/Dynamic and Monte Carlo Modeling
    2016
    Co-Authors: Ans Punt, Benoît Schilter, Peter J. Van Bladeren, Alicia Paini, A. Spenkelink, Gabriele Scholz, Ivonne M.c.m. Rietjens
    Abstract:

    Estragole is a known hepatocarcinogen in rodents at high doses following metabolic conversion to the DNA-reactive metabolite 1′-sulfooxyestragole. The aim of the present study was to model possible levels of DNA adduct formation in (individual) humans upon exposure to estragole. This was done by extending a previously defined PBK model for estragole in humans to include (i) new data on interindividual variation in the kinetics for the major PBK model parameters influencing the formation of 1′-sulfooxyestragole, (ii) an equation describing the relationship between 1′-sulfooxyestragole and DNA adduct formation, (iii) Monte Carlo modeling to simulate interindividual human variation in DNA adduct formation in the population, and (iv) a comparison of the predictions made to human data on DNA adduct formation for the related alkenylbenzene Methyleugenol. Adequate model predictions could be made, with the predicted DNA adduct levels at the estimated daily intake of estragole of 0.01 mg/kg bw ranging between 1.6 and 8.8 adducts in 108 nucleotides (nts) (50th and 99th percentiles, respectively). This is somewhat lower than values reported in the literature for the related alkenylbenzene Methyleugenol in surgical human liver samples. The predicted levels seem to be below DNA adduct levels that are linked with tumor formation by alkenylbenzenes in rodents, which were estimated to amount to 188–500 adducts per 108 nts at the BMD10 values of estragole and Methyleugenol. Although this does not seem to point to a significant health concern for human dietary exposure, drawing firm conclusions may have to await further validation of the model’s predictions

  • Physiologically based kinetic models for the alkenylbenzene elemicin in rat and human and possible implications for risk assessment.
    Chemical research in toxicology, 2012
    Co-Authors: Suzanne J. P. L. Van Den Berg, Jacques Vervoort, Ans Punt, Ans E. M. F. Soffers, Stephen Ngeleja, Bert Spenkelink, Ivonne M.c.m. Rietjens
    Abstract:

    The present study describes physiologically based kinetic (PBK) models for the alkenylbenzene elemicin (3,4,5-trimethoxyallylbenzene) in rat and human, based on the PBK models previously developed for the structurally related alkenylbenzenes estragole, Methyleugenol, and safrole. Using the newly developed models, the level of metabolic activation of elemicin in rat and human was predicted to obtain insight in species differences in the bioactivation of elemicin and read across to the other methoxy allylbenzenes, estragole and Methyleugenol. Results reveal that the differences between rat and human in the formation of the proximate carcinogenic metabolite 1'-hydroxyelemicin and the ultimate carcinogenic metabolite 1'-sulfoxyelemicin are limited (

  • Physiologically Based Kinetic Models for the Alkenylbenzene Elemicin in Rat and Human and Possible Implications for Risk Assessment
    2012
    Co-Authors: Suzanne J. P. L. Van Den Berg, Jacques Vervoort, Ans Punt, Ans E. M. F. Soffers, Stephen Ngeleja, Bert Spenkelink, Ivonne M.c.m. Rietjens
    Abstract:

    The present study describes physiologically based kinetic (PBK) models for the alkenylbenzene elemicin (3,4,5-trimethoxyallylbenzene) in rat and human, based on the PBK models previously developed for the structurally related alkenylbenzenes estragole, Methyleugenol, and safrole. Using the newly developed models, the level of metabolic activation of elemicin in rat and human was predicted to obtain insight in species differences in the bioactivation of elemicin and read across to the other methoxy allylbenzenes, estragole and Methyleugenol. Results reveal that the differences between rat and human in the formation of the proximate carcinogenic metabolite 1′-hydroxyelemicin and the ultimate carcinogenic metabolite 1′-sulfoxyelemicin are limited (

Supriyadi Supriyadi - One of the best experts on this subject based on the ideXlab platform.

  • Perbandingan Ekstraksi Oleoresin Biji Pala (Myrictica Fragrans Houtt) Asal Maluku Utara Menggunakan Metode Maserasi dan Gabungan Distilasi – Maserasi
    2013
    Co-Authors: Muhammad Assagaf, Chusnul Hidayat, Pudji Hastuti, Supriyadi Supriyadi
    Abstract:

    The purpose of this study was to compare the composition of oleoresin nutmeg (Myristica fragrans Houtt) madedirectly by maceration and combined distillation – maceration. Nutmeg oleoresin yield amounted to 15.17±0.07 (% db)obtained by direct maceration and oleoresin extraction method combined distillation and maceration obtained yield of20.07±0.23 (% db). While the essential oil yield itself from the water-steam distillation of the results obtained at 6.61(% db). Ethanol extract of oleoresin analysis using GCMS method identiÞ ed a total of 39 kinds of compounds withthe composition of major components is Methyleugenol (33.40 %), myristicine (10.90 %), cis-methyl isoeugenol (9.09%), elemicin (8.33 %), and isocoumarin (5.61 %). For nutmeg essential oil contained 31 components of the compound,where the components are located in large numbers was sabinene (34.97 %), !-phellandrene (19.9 %), Methyleugenol(7.55 %), myristicine (5.29 %) and elimicine (3.21 %). As for the essential oil is mixed with the oleoresin from the pulpremaining distillation contained 58 components that make up the compound of the oleoresin with a mixture of maincomponents, namely: sabinene (12.38 %) myristicine (10.88 %), elemicin (8.93 %), isocoumarin (6.26 %), myristicacid (5.96 %), and "-pinene (4.73 %). ABSTRAK Penelitian ini bertujuan untuk membandingkan komponen penyusun oleoresin biji pala (Myristica fragrans Houtt)yang dibuat dengan cara maserasi langsung dan gabungan distilasi – maserasi. Yield oleoresin pala sebesar 15,17±0,07(% bk) yang diperoleh dengan cara maserasi langsung dan oleoresin hasil ekstraksi gabungan metode distilasi danmaserasi diperoleh yield sebesar 20,07±0,23 (% bk). Sedangkan yield minyak atsiri sendiri dari hasil distilasi air-uapdiperoleh sebesar 6,61 (% bk). Senyawa penyusun oleoresin ekstrak etanol hasil analisis menggunakan metode GCMSteridentiÞ kasi senyawa sebanyak 39 macam dengan komponen yang berada dalam jumlah besar adalah Methyleugenol(33,40 %), myristicine (10,90 %), cis-methyl isoeugenol (9,09 %), elemicin (8,33 %), dan isocoumarin (5,61 %). Untukminyak atsiri biji pala terdapat 31 komponen senyawa, dimana komponen yang berada dalam jumlah yang besar adalahsabinene (34,97 %), !– phellandrene (9,19 %), Methyleugenol (7,55 %), myristicine (5,29 %) dan elimicine (3,21%).Sedangkan untuk minyak atsiri yang dicampur dengan oleoresin dari ampas sisa distilasi terdapat 58 komponen senyawayang menyusun oleoresin campuran tersebut dengan senyawa yang berada dalam jumlah besar yaitu; sabinene (12,38%) myristicine (10,88 %), elemicin (8,93 %), isocoumarin (6,26 %), myristic acid (5,96 %), dan !- pinene (4,73 %).

  • PERBANDINGAN EKSTRAKSI OLEORESIN BIJI PALA (MYRICTICA FRAGRANS HOUTT) ASAL MALUKU UTARA MENGGUNAKAN METODE MASERASI DAN GABUNGAN DISTILASI – MASERASI
    Universitas Gadjah Mada, 2013
    Co-Authors: Muhammad Assagaf, Chusnul Hidayat, Pudji Hastuti, Supriyadi Supriyadi
    Abstract:

    PERBANDINGAN EKSTRAKSI OLEORESIN BIJI PALA (MYRICTICA FRAGRANSHOUTT) ASAL MALUKU UTARA MENGGUNAKAN METODE MASERASI DANGABUNGAN DISTILASI – MASERASI Comparison of Nutmeg (Myristica fragrans Houtt) Oleoresin Extraction from North Maluku UsingMaceration and Combination of Distillation-Maceration Methods Muhammad Assagaf1, Pudji Hastuti2, Chusnul Hidayat2, Supriyadi2 1 Balai Pengkajian Teknologi Pertanian (BPTP) Maluku Utara, Jl. Kusu, SoÞ Þ , Kota Tidore Kepulauan 2 Fakultas Teknologi Pertanian Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281 Email: assagaf_met@yahoo.com ABSTRAK Penelitian ini bertujuan untuk membandingkan komponen penyusun oleoresin biji pala (Myristica fragrans Houtt)yang dibuat dengan cara maserasi langsung dan gabungan distilasi – maserasi. Yield oleoresin pala sebesar 15,17±0,07(% bk) yang diperoleh dengan cara maserasi langsung dan oleoresin hasil ekstraksi gabungan metode distilasi danmaserasi diperoleh yield sebesar 20,07±0,23 (% bk). Sedangkan yield minyak atsiri sendiri dari hasil distilasi air-uapdiperoleh sebesar 6,61 (% bk). Senyawa penyusun oleoresin ekstrak etanol hasil analisis menggunakan metode GCMSteridentiÞ kasi senyawa sebanyak 39 macam dengan komponen yang berada dalam jumlah besar adalah Methyleugenol(33,40 %), myristicine (10,90 %), cis-methyl isoeugenol (9,09 %), elemicin (8,33 %), dan isocoumarin (5,61 %). Untukminyak atsiri biji pala terdapat 31 komponen senyawa, dimana komponen yang berada dalam jumlah yang besar adalahsabinene (34,97 %), !– phellandrene (9,19 %), Methyleugenol (7,55 %), myristicine (5,29 %) dan elimicine (3,21%).Sedangkan untuk minyak atsiri yang dicampur dengan oleoresin dari ampas sisa distilasi terdapat 58 komponen senyawayang menyusun oleoresin campuran tersebut dengan senyawa yang berada dalam jumlah besar yaitu; sabinene (12,38%) myristicine (10,88 %), elemicin (8,93 %), isocoumarin (6,26 %), myristic acid (5,96 %), dan !- pinene (4,73 %).Kata kunci: minyak atsiri, oleoresin, maserasi, distilasi-maserasi, GC-MS ABSTRACT The purpose of this study was to compare the composition of oleoresin nutmeg (Myristica fragrans Houtt) madedirectly by maceration and combined distillation – maceration. Nutmeg oleoresin yield amounted to 15.17±0.07 (% db)obtained by direct maceration and oleoresin extraction method combined distillation and maceration obtained yield of20.07±0.23 (% db). While the essential oil yield itself from the water-steam distillation of the results obtained at 6.61(% db). Ethanol extract of oleoresin analysis using GCMS method identiÞ ed a total of 39 kinds of compounds withthe composition of major components is Methyleugenol (33.40 %), myristicine (10.90 %), cis-methyl isoeugenol (9.09%), elemicin (8.33 %), and isocoumarin (5.61 %). For nutmeg essential oil contained 31 components of the compound,where the components are located in large numbers was sabinene (34.97 %), !-phellandrene (19.9 %), Methyleugenol(7.55 %), myristicine (5.29 %) and elimicine (3.21 %). As for the essential oil is mixed with the oleoresin from the pulpremaining distillation contained 58 components that make up the compound of the oleoresin with a mixture of maincomponents, namely: sabinene (12.38 %) myristicine (10.88 %), elemicin (8.93 %), isocoumarin (6.26 %), myristicacid (5.96 %), and "-pinene (4.73 %).Key words: essential oil, oleoresin, maceration, maceration-distillation, GC-M

Muhammad Assagaf - One of the best experts on this subject based on the ideXlab platform.

  • Perbandingan Ekstraksi Oleoresin Biji Pala (Myrictica Fragrans Houtt) Asal Maluku Utara Menggunakan Metode Maserasi dan Gabungan Distilasi – Maserasi
    2013
    Co-Authors: Muhammad Assagaf, Chusnul Hidayat, Pudji Hastuti, Supriyadi Supriyadi
    Abstract:

    The purpose of this study was to compare the composition of oleoresin nutmeg (Myristica fragrans Houtt) madedirectly by maceration and combined distillation – maceration. Nutmeg oleoresin yield amounted to 15.17±0.07 (% db)obtained by direct maceration and oleoresin extraction method combined distillation and maceration obtained yield of20.07±0.23 (% db). While the essential oil yield itself from the water-steam distillation of the results obtained at 6.61(% db). Ethanol extract of oleoresin analysis using GCMS method identiÞ ed a total of 39 kinds of compounds withthe composition of major components is Methyleugenol (33.40 %), myristicine (10.90 %), cis-methyl isoeugenol (9.09%), elemicin (8.33 %), and isocoumarin (5.61 %). For nutmeg essential oil contained 31 components of the compound,where the components are located in large numbers was sabinene (34.97 %), !-phellandrene (19.9 %), Methyleugenol(7.55 %), myristicine (5.29 %) and elimicine (3.21 %). As for the essential oil is mixed with the oleoresin from the pulpremaining distillation contained 58 components that make up the compound of the oleoresin with a mixture of maincomponents, namely: sabinene (12.38 %) myristicine (10.88 %), elemicin (8.93 %), isocoumarin (6.26 %), myristicacid (5.96 %), and "-pinene (4.73 %). ABSTRAK Penelitian ini bertujuan untuk membandingkan komponen penyusun oleoresin biji pala (Myristica fragrans Houtt)yang dibuat dengan cara maserasi langsung dan gabungan distilasi – maserasi. Yield oleoresin pala sebesar 15,17±0,07(% bk) yang diperoleh dengan cara maserasi langsung dan oleoresin hasil ekstraksi gabungan metode distilasi danmaserasi diperoleh yield sebesar 20,07±0,23 (% bk). Sedangkan yield minyak atsiri sendiri dari hasil distilasi air-uapdiperoleh sebesar 6,61 (% bk). Senyawa penyusun oleoresin ekstrak etanol hasil analisis menggunakan metode GCMSteridentiÞ kasi senyawa sebanyak 39 macam dengan komponen yang berada dalam jumlah besar adalah Methyleugenol(33,40 %), myristicine (10,90 %), cis-methyl isoeugenol (9,09 %), elemicin (8,33 %), dan isocoumarin (5,61 %). Untukminyak atsiri biji pala terdapat 31 komponen senyawa, dimana komponen yang berada dalam jumlah yang besar adalahsabinene (34,97 %), !– phellandrene (9,19 %), Methyleugenol (7,55 %), myristicine (5,29 %) dan elimicine (3,21%).Sedangkan untuk minyak atsiri yang dicampur dengan oleoresin dari ampas sisa distilasi terdapat 58 komponen senyawayang menyusun oleoresin campuran tersebut dengan senyawa yang berada dalam jumlah besar yaitu; sabinene (12,38%) myristicine (10,88 %), elemicin (8,93 %), isocoumarin (6,26 %), myristic acid (5,96 %), dan !- pinene (4,73 %).

  • PERBANDINGAN EKSTRAKSI OLEORESIN BIJI PALA (MYRICTICA FRAGRANS HOUTT) ASAL MALUKU UTARA MENGGUNAKAN METODE MASERASI DAN GABUNGAN DISTILASI – MASERASI
    Universitas Gadjah Mada, 2013
    Co-Authors: Muhammad Assagaf, Chusnul Hidayat, Pudji Hastuti, Supriyadi Supriyadi
    Abstract:

    PERBANDINGAN EKSTRAKSI OLEORESIN BIJI PALA (MYRICTICA FRAGRANSHOUTT) ASAL MALUKU UTARA MENGGUNAKAN METODE MASERASI DANGABUNGAN DISTILASI – MASERASI Comparison of Nutmeg (Myristica fragrans Houtt) Oleoresin Extraction from North Maluku UsingMaceration and Combination of Distillation-Maceration Methods Muhammad Assagaf1, Pudji Hastuti2, Chusnul Hidayat2, Supriyadi2 1 Balai Pengkajian Teknologi Pertanian (BPTP) Maluku Utara, Jl. Kusu, SoÞ Þ , Kota Tidore Kepulauan 2 Fakultas Teknologi Pertanian Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281 Email: assagaf_met@yahoo.com ABSTRAK Penelitian ini bertujuan untuk membandingkan komponen penyusun oleoresin biji pala (Myristica fragrans Houtt)yang dibuat dengan cara maserasi langsung dan gabungan distilasi – maserasi. Yield oleoresin pala sebesar 15,17±0,07(% bk) yang diperoleh dengan cara maserasi langsung dan oleoresin hasil ekstraksi gabungan metode distilasi danmaserasi diperoleh yield sebesar 20,07±0,23 (% bk). Sedangkan yield minyak atsiri sendiri dari hasil distilasi air-uapdiperoleh sebesar 6,61 (% bk). Senyawa penyusun oleoresin ekstrak etanol hasil analisis menggunakan metode GCMSteridentiÞ kasi senyawa sebanyak 39 macam dengan komponen yang berada dalam jumlah besar adalah Methyleugenol(33,40 %), myristicine (10,90 %), cis-methyl isoeugenol (9,09 %), elemicin (8,33 %), dan isocoumarin (5,61 %). Untukminyak atsiri biji pala terdapat 31 komponen senyawa, dimana komponen yang berada dalam jumlah yang besar adalahsabinene (34,97 %), !– phellandrene (9,19 %), Methyleugenol (7,55 %), myristicine (5,29 %) dan elimicine (3,21%).Sedangkan untuk minyak atsiri yang dicampur dengan oleoresin dari ampas sisa distilasi terdapat 58 komponen senyawayang menyusun oleoresin campuran tersebut dengan senyawa yang berada dalam jumlah besar yaitu; sabinene (12,38%) myristicine (10,88 %), elemicin (8,93 %), isocoumarin (6,26 %), myristic acid (5,96 %), dan !- pinene (4,73 %).Kata kunci: minyak atsiri, oleoresin, maserasi, distilasi-maserasi, GC-MS ABSTRACT The purpose of this study was to compare the composition of oleoresin nutmeg (Myristica fragrans Houtt) madedirectly by maceration and combined distillation – maceration. Nutmeg oleoresin yield amounted to 15.17±0.07 (% db)obtained by direct maceration and oleoresin extraction method combined distillation and maceration obtained yield of20.07±0.23 (% db). While the essential oil yield itself from the water-steam distillation of the results obtained at 6.61(% db). Ethanol extract of oleoresin analysis using GCMS method identiÞ ed a total of 39 kinds of compounds withthe composition of major components is Methyleugenol (33.40 %), myristicine (10.90 %), cis-methyl isoeugenol (9.09%), elemicin (8.33 %), and isocoumarin (5.61 %). For nutmeg essential oil contained 31 components of the compound,where the components are located in large numbers was sabinene (34.97 %), !-phellandrene (19.9 %), Methyleugenol(7.55 %), myristicine (5.29 %) and elimicine (3.21 %). As for the essential oil is mixed with the oleoresin from the pulpremaining distillation contained 58 components that make up the compound of the oleoresin with a mixture of maincomponents, namely: sabinene (12.38 %) myristicine (10.88 %), elemicin (8.93 %), isocoumarin (6.26 %), myristicacid (5.96 %), and "-pinene (4.73 %).Key words: essential oil, oleoresin, maceration, maceration-distillation, GC-M