Microanalysis

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Ann Van Schepdael - One of the best experts on this subject based on the ideXlab platform.

  • advances in capillary electrophoretically mediated Microanalysis for on line enzymatic and derivatization reactions
    Electrophoresis, 2018
    Co-Authors: Shengyun Huang, Prasanta Paul, Pranov Ramana, Erwin Adams, Patrick Augustijns, Ann Van Schepdael
    Abstract:

    This review summarizes recent developments, applications and innovations of capillary electrophoretically mediated Microanalysis methods. As a follow up of an earlier review, it covers the literature from early 2015 - early 2017. This article is divided into three parts. In the first part, different types of mixing procedures and applications of enzyme mediated Microanalysis are discussed; the second part summarizes immobilized enzyme reactors (IMERs), while the third part deals with recent advances in on-line derivatization reactions. This article is protected by copyright. All rights reserved

  • recent advances in ce mediated Microanalysis for enzyme study
    Electrophoresis, 2014
    Co-Authors: Xu Wang, Erwin Adams, Kefeng Li, Ann Van Schepdael
    Abstract:

    This review gives an overview of the recent developments and applications in the use of CE-mediated Microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated Microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.

  • Electrophoretically mediated Microanalysis
    Journal of Chromatography A, 2004
    Co-Authors: Soňa Nováková, Sigrid Van Dyck, Ann Van Schepdael, Jos Hoogmartens, Zdeněk Glatz
    Abstract:

    This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated Microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.

Elena Bonanno - One of the best experts on this subject based on the ideXlab platform.

  • energy dispersive x ray edx Microanalysis a powerful tool in biomedical research and diagnosis
    European Journal of Histochemistry, 2018
    Co-Authors: Manuel Scimeca, Simone Bischetti, Harpreet Kaur Lamsira, Rita Bonfiglio, Elena Bonanno
    Abstract:

    The Energy Dispersive X-ray (EDX) Microanalysis is a technique of elemental analysis associated to electron microscopy based on the generation of characteristic Xrays that reveals the presence of elements present in the specimens. The EDX Microanalysis is used in different biomedical fields by many researchers and clinicians. Nevertheless, most of the scientific community is not fully aware of its possible applications. The spectrum of EDX Microanalysis contains both semi-qualitative and semi-quantitative information. EDX technique is made useful in the study of drugs, such as in the study of drugs delivery in which the EDX is an important tool to detect nanoparticles (generally, used to improve the therapeutic performance of some chemotherapeutic agents). EDX is also used in the study of environmental pollution and in the characterization of mineral bioaccumulated in the tissues. In conclusion, the EDX can be considered as a useful tool in all works that require element determination, endogenous or exogenous, in the tissue, cell or any other sample.

  • assessment of metal contaminants in non small cell lung cancer by edx Microanalysis
    European Journal of Histochemistry, 2014
    Co-Authors: Manuel Scimeca, Simone Bischetti, Augusto Orlandi, Irene Terrenato, Elena Bonanno
    Abstract:

    Human cardio-respiratory diseases are strongly correlated to concentrations of atmospheric elements. Bioaccumulation of heavy metals is strictly monitored, because of its possible toxic effects. In this work, we utilized the EDX Microanalysis in order to identify the potential heavy metal accumulation in the lung tissue. To this aim, we enrolled 45 human lung biopsies: 15 non-small cell lung cancers, 15 lung benign lesions and 15 control biopsies. Lung samples were both paraffin embedded for light microscopy study and epon-epoxid embedded for transmission electron microscopy. EDX Microanalysis was performed on 100 nm thick unstained ultrathin-sections placed on specific copper grids. Our results demonstrated that the EDX technology was particularly efficient in the study of elemental composition of lung tissues, where we found heavy metals, such as Cobalt (Co), Chromium (Cr), Manganese (Mn) and Lead (Pb). Furthermore, in malignant lesions we demonstrated the presence of multiple bio-accumulated elements. In fact, a high rate of lung cancers was associated with the presence of 3 or more bio-accumulated elements compared to benign lesions and control tissue (91.7%, 0%, 8.3%, respectively). The environmental impact on pulmonary carcinogenesis could be better clarified by demonstrating the presence of polluting agents in lung tissues. The application of EDX Microanalysis on biological tissues could shed new light in the study of the possible bioaccumulation of polluting agents in different human organs and systems.

Wei He - One of the best experts on this subject based on the ideXlab platform.

  • the 773 k isothermal section of the phase diagram of ternary ni ti y system
    Journal of Alloys and Compounds, 2000
    Co-Authors: Yinghong Zhuang, Wei He
    Abstract:

    Abstract The 773 K isothermal section of the Ni–Ti–Y system was investigated by X-ray powder diffraction, differential thermal Microanalysis and electron-probe Microanalysis techniques. It consists of 15 single-phase regions, 28 two-phase regions and 13 three-phase regions. At 773 K, the maximum solubilities of Y in Ni and Ti in Ni 2 Y, Ni 3 Y and Ni 5 Y are about 5.0, 3.0, 2.0 and 5.0 at.%, respectively. In the Ni–Ti binary system, the composition ranges of the phase α-(Ni) was determined as 0–11.0 at.% Ti.

Yinghong Zhuang - One of the best experts on this subject based on the ideXlab platform.

  • the 773 k isothermal section of the phase diagram of ternary ni ti y system
    Journal of Alloys and Compounds, 2000
    Co-Authors: Yinghong Zhuang, Wei He
    Abstract:

    Abstract The 773 K isothermal section of the Ni–Ti–Y system was investigated by X-ray powder diffraction, differential thermal Microanalysis and electron-probe Microanalysis techniques. It consists of 15 single-phase regions, 28 two-phase regions and 13 three-phase regions. At 773 K, the maximum solubilities of Y in Ni and Ti in Ni 2 Y, Ni 3 Y and Ni 5 Y are about 5.0, 3.0, 2.0 and 5.0 at.%, respectively. In the Ni–Ti binary system, the composition ranges of the phase α-(Ni) was determined as 0–11.0 at.% Ti.

  • The isothermal section (500 °C) of the phase diagram of the ternary system GeHoSi
    Journal of The Less Common Metals, 1991
    Co-Authors: Yinghong Zhuang, Junqin Li
    Abstract:

    Abstract The isothermal section (500 °C) of the phase diagram of the ternary system Cu-Sn-Y (40 at.% Y or less) was investigated by X-ray diffraction, differential thermal Microanalysis, optical Microanalysis and electron probe Microanalysis techniques. The isothermal section consists of 12 single-phase regions, 25 two-phase regions and 14 three-phase regions. Three new ternary compounds were found in this isothermal section: the θ phase (Y8Cu7Sn5), the β phase (Y3Cu4Sn3) and the γ phase (Y19Cu66Sn15). They are all non-variable compounds. The θ phase is an orthorhombic system with a = 9.702 A , b = 7.285 A and c = 6.545 A . The maximum solid solubility of tin in α-Cu at 500 °C is about 9.0 at.% and that of yttrium in α-Cu at 500 °C (with 4.3 at.% Sn) is about 1.5 at.%.

Manuel Scimeca - One of the best experts on this subject based on the ideXlab platform.

  • energy dispersive x ray edx Microanalysis a powerful tool in biomedical research and diagnosis
    European Journal of Histochemistry, 2018
    Co-Authors: Manuel Scimeca, Simone Bischetti, Harpreet Kaur Lamsira, Rita Bonfiglio, Elena Bonanno
    Abstract:

    The Energy Dispersive X-ray (EDX) Microanalysis is a technique of elemental analysis associated to electron microscopy based on the generation of characteristic Xrays that reveals the presence of elements present in the specimens. The EDX Microanalysis is used in different biomedical fields by many researchers and clinicians. Nevertheless, most of the scientific community is not fully aware of its possible applications. The spectrum of EDX Microanalysis contains both semi-qualitative and semi-quantitative information. EDX technique is made useful in the study of drugs, such as in the study of drugs delivery in which the EDX is an important tool to detect nanoparticles (generally, used to improve the therapeutic performance of some chemotherapeutic agents). EDX is also used in the study of environmental pollution and in the characterization of mineral bioaccumulated in the tissues. In conclusion, the EDX can be considered as a useful tool in all works that require element determination, endogenous or exogenous, in the tissue, cell or any other sample.

  • assessment of metal contaminants in non small cell lung cancer by edx Microanalysis
    European Journal of Histochemistry, 2014
    Co-Authors: Manuel Scimeca, Simone Bischetti, Augusto Orlandi, Irene Terrenato, Elena Bonanno
    Abstract:

    Human cardio-respiratory diseases are strongly correlated to concentrations of atmospheric elements. Bioaccumulation of heavy metals is strictly monitored, because of its possible toxic effects. In this work, we utilized the EDX Microanalysis in order to identify the potential heavy metal accumulation in the lung tissue. To this aim, we enrolled 45 human lung biopsies: 15 non-small cell lung cancers, 15 lung benign lesions and 15 control biopsies. Lung samples were both paraffin embedded for light microscopy study and epon-epoxid embedded for transmission electron microscopy. EDX Microanalysis was performed on 100 nm thick unstained ultrathin-sections placed on specific copper grids. Our results demonstrated that the EDX technology was particularly efficient in the study of elemental composition of lung tissues, where we found heavy metals, such as Cobalt (Co), Chromium (Cr), Manganese (Mn) and Lead (Pb). Furthermore, in malignant lesions we demonstrated the presence of multiple bio-accumulated elements. In fact, a high rate of lung cancers was associated with the presence of 3 or more bio-accumulated elements compared to benign lesions and control tissue (91.7%, 0%, 8.3%, respectively). The environmental impact on pulmonary carcinogenesis could be better clarified by demonstrating the presence of polluting agents in lung tissues. The application of EDX Microanalysis on biological tissues could shed new light in the study of the possible bioaccumulation of polluting agents in different human organs and systems.