The Experts below are selected from a list of 9864 Experts worldwide ranked by ideXlab platform
Xiangfeng Huang - One of the best experts on this subject based on the ideXlab platform.
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bioconversion of mixed volatile fatty acids into Microbial Lipids by cryptococcus curvatus atcc 20509
Bioresource Technology, 2017Co-Authors: Ming Yuan, Xiangfeng HuangAbstract:Abstract The oleaginous yeast Cryptococcus curvatus ATCC 20509 can use 5–40 g/L of acetic, propionic, or butyric acid as sole carbon source to produce Lipids. High concentrations (30 g/L) of mixed volatile fatty acids (VFAs) were used to cultivate C. curvatus to explore the effects of different ratios of mixed VFAs on lipid production and composition. When mixed VFAs (VFA ratio was 15:5:10) were used as carbon sources, the highest cell mass and lipid concentration were 8.68 g/L and 4.93 g/L, respectively, which were significantly higher than those when 30 g/L of acetic acid was used as sole carbon source. The highest content and yield of odd-numbered fatty acids were 45.1% (VFA ratio was 0:15:15) and 1.62 g/L (VFA ratio was 5:15:10), respectively. These results indicate that adjusting the composition ratios of mixed VFAs effectively improves Microbial lipid synthesis and the yield of odd-numbered fatty acids.
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efficient bioconversion of high content volatile fatty acids into Microbial Lipids by cryptococcus curvatus atcc 20509
Bioresource Technology, 2017Co-Authors: Xiangfeng Huang, Rui Chen, Ming YuanAbstract:Abstract The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600 = 5.1 improved the acetic acid utilization ratio (99.8%) and Lipids production (4.63 g/L) in mediums with 30 g/L of acetic acid. For the higher acetic acid concentration (40 g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH = 8) was superior over improvement in the inoculate, with the lipid production increased from 1.08 g/L to 6.49 g/L. Subsequently, mixed VFAs at concentrations of 30 g/L and 40 g/L were used as the carbon source to simulate waste-derived VFAs. High lipid production (4.82 and 7.45 g/L, respectively) was correspondingly achieved with similar high lipid yield (0.187 g/g). This study provides an effective strategy to enhance the bioconversion of high-content VFAs into Microbial Lipids.
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Microbial conversion of mixed volatile fatty acids into Microbial Lipids by sequencing batch culture strategy
Bioresource Technology, 2016Co-Authors: Ming Yuan, Lijun Lu, Kaiming Peng, Xiangfeng HuangAbstract:Abstract Four mixed volatile fatty acids (VFAs) were used as sole carbon source to culture oleaginous yeast Cryptococcus curvatus by sequencing batch culture strategy. The highest lipid content (42.7%) and concentration (1.77 g/L) were achieved when the ratio of VFAs (acetic, propionic, and butyric acids) was 6:3:1. The oleaginous yeast favored to use VFAs for lipid biosynthesis rather than cell proliferation. With regard to the utilization ratio of VFAs, acetic acid reached over 99%, whereas propionic acid was barely 35%. The produced Lipids contained nearly 45% of monounsaturated fatty acids, which can be the ideal raw materials for biodiesel production. Additionally, the produced odd-numbered fatty acid content reached 23.6% when the propionate acid content of VFAs was 50%. Further analysis showed that increasing the ratio of acetic acid was most beneficial to cell mass and lipid production, whereas propionic acid and butyric acid were more conducive to lipid and cell mass synthesis, respectively.
Ming Yuan - One of the best experts on this subject based on the ideXlab platform.
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bioconversion of mixed volatile fatty acids into Microbial Lipids by cryptococcus curvatus atcc 20509
Bioresource Technology, 2017Co-Authors: Ming Yuan, Xiangfeng HuangAbstract:Abstract The oleaginous yeast Cryptococcus curvatus ATCC 20509 can use 5–40 g/L of acetic, propionic, or butyric acid as sole carbon source to produce Lipids. High concentrations (30 g/L) of mixed volatile fatty acids (VFAs) were used to cultivate C. curvatus to explore the effects of different ratios of mixed VFAs on lipid production and composition. When mixed VFAs (VFA ratio was 15:5:10) were used as carbon sources, the highest cell mass and lipid concentration were 8.68 g/L and 4.93 g/L, respectively, which were significantly higher than those when 30 g/L of acetic acid was used as sole carbon source. The highest content and yield of odd-numbered fatty acids were 45.1% (VFA ratio was 0:15:15) and 1.62 g/L (VFA ratio was 5:15:10), respectively. These results indicate that adjusting the composition ratios of mixed VFAs effectively improves Microbial lipid synthesis and the yield of odd-numbered fatty acids.
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efficient bioconversion of high content volatile fatty acids into Microbial Lipids by cryptococcus curvatus atcc 20509
Bioresource Technology, 2017Co-Authors: Xiangfeng Huang, Rui Chen, Ming YuanAbstract:Abstract The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600 = 5.1 improved the acetic acid utilization ratio (99.8%) and Lipids production (4.63 g/L) in mediums with 30 g/L of acetic acid. For the higher acetic acid concentration (40 g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH = 8) was superior over improvement in the inoculate, with the lipid production increased from 1.08 g/L to 6.49 g/L. Subsequently, mixed VFAs at concentrations of 30 g/L and 40 g/L were used as the carbon source to simulate waste-derived VFAs. High lipid production (4.82 and 7.45 g/L, respectively) was correspondingly achieved with similar high lipid yield (0.187 g/g). This study provides an effective strategy to enhance the bioconversion of high-content VFAs into Microbial Lipids.
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Microbial conversion of mixed volatile fatty acids into Microbial Lipids by sequencing batch culture strategy
Bioresource Technology, 2016Co-Authors: Ming Yuan, Lijun Lu, Kaiming Peng, Xiangfeng HuangAbstract:Abstract Four mixed volatile fatty acids (VFAs) were used as sole carbon source to culture oleaginous yeast Cryptococcus curvatus by sequencing batch culture strategy. The highest lipid content (42.7%) and concentration (1.77 g/L) were achieved when the ratio of VFAs (acetic, propionic, and butyric acids) was 6:3:1. The oleaginous yeast favored to use VFAs for lipid biosynthesis rather than cell proliferation. With regard to the utilization ratio of VFAs, acetic acid reached over 99%, whereas propionic acid was barely 35%. The produced Lipids contained nearly 45% of monounsaturated fatty acids, which can be the ideal raw materials for biodiesel production. Additionally, the produced odd-numbered fatty acid content reached 23.6% when the propionate acid content of VFAs was 50%. Further analysis showed that increasing the ratio of acetic acid was most beneficial to cell mass and lipid production, whereas propionic acid and butyric acid were more conducive to lipid and cell mass synthesis, respectively.
Zongbao K. Zhao - One of the best experts on this subject based on the ideXlab platform.
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catalytic hydrodeoxygenation of methyl stearate and Microbial Lipids to diesel range alkanes over pd hpa sio2 catalysts
Industrial & Engineering Chemistry Research, 2020Co-Authors: Huifang Liu, Hongwei Shen, Jianyu Han, Qitian Huang, Lijun Lei, Zhipeng Huang, Zhixin Zhang, Zongbao K. ZhaoAbstract:Catalytic hydrotreating of renewable oils is a promising way to get diesel alkanes, with the use of Microbial Lipids further facilitating scalable production of green diesel. The core issue of trig...
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recycling biodiesel derived glycerol by the oleaginous yeast rhodosporidium toruloides y4 through the two stage lipid production process
Biochemical Engineering Journal, 2014Co-Authors: Hongwei Shen, Zhiwei Gong, Xiaobing Yang, Zongbao K. ZhaoAbstract:Abstract Direct utilization of crude glycerol, a major byproduct in biodiesel industry, becomes imperative, because its production has outpaced the demand recently. We demonstrated that the oleaginous yeast Rhodosporidium toruloides Y4 had a great capacity to convert glycerol into Lipids with high yield using the two-stage production process. Significantly higher cell mass and lipid yield were observed when the media were made with synthetic crude glycerol than pure glycerol. The process achieved a lipid yield of 0.22 g g −1 glycerol, which was comparable with the lipid yield using glucose as the substrate. Lipid samples showed similar fatty acid compositional profiles to those of vegetable oils, suggesting that such Microbial Lipids were potential feedstock for biodiesel production. Our data provided an attractive route to integrate biodiesel production with Microbial lipid technology for better resource efficiency and economical viability.
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efficient conversion of biomass into Lipids by using the simultaneous saccharification and enhanced lipid production process
Biotechnology for Biofuels, 2013Co-Authors: Zhiwei Gong, Hongwei Shen, Haibo Xie, Qian Wang, Xiaobing Yang, Zongbao K. ZhaoAbstract:Background Microbial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce Lipids. However, the costs of Microbial Lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of Microbial lipid technology.
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Enzyme-assisted extraction of Lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides
Bioresource technology, 2012Co-Authors: Guojie Jin, Fan Yang, Hongwei Shen, Zongbao K. ZhaoAbstract:Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of Microbial Lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of Lipids from the culture of the yeast Rhodosporidium toruloides was carried out. With a heat pre-treatment with microwave, enzymatic treatment with the recombinant β-1,3-glucomannanase, plMAN5C, and extraction with ethyl acetate, 96.6% of the total Lipids were extracted from R. toruloides cells at room temperature and atmospheric pressure directly from the culture without dewatering. Therefore, this process could significantly reduce energy consumption and costs for Lipids extraction from the yeast.
Hongwei Shen - One of the best experts on this subject based on the ideXlab platform.
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catalytic hydrodeoxygenation of methyl stearate and Microbial Lipids to diesel range alkanes over pd hpa sio2 catalysts
Industrial & Engineering Chemistry Research, 2020Co-Authors: Huifang Liu, Hongwei Shen, Jianyu Han, Qitian Huang, Lijun Lei, Zhipeng Huang, Zhixin Zhang, Zongbao K. ZhaoAbstract:Catalytic hydrotreating of renewable oils is a promising way to get diesel alkanes, with the use of Microbial Lipids further facilitating scalable production of green diesel. The core issue of trig...
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recycling biodiesel derived glycerol by the oleaginous yeast rhodosporidium toruloides y4 through the two stage lipid production process
Biochemical Engineering Journal, 2014Co-Authors: Hongwei Shen, Zhiwei Gong, Xiaobing Yang, Zongbao K. ZhaoAbstract:Abstract Direct utilization of crude glycerol, a major byproduct in biodiesel industry, becomes imperative, because its production has outpaced the demand recently. We demonstrated that the oleaginous yeast Rhodosporidium toruloides Y4 had a great capacity to convert glycerol into Lipids with high yield using the two-stage production process. Significantly higher cell mass and lipid yield were observed when the media were made with synthetic crude glycerol than pure glycerol. The process achieved a lipid yield of 0.22 g g −1 glycerol, which was comparable with the lipid yield using glucose as the substrate. Lipid samples showed similar fatty acid compositional profiles to those of vegetable oils, suggesting that such Microbial Lipids were potential feedstock for biodiesel production. Our data provided an attractive route to integrate biodiesel production with Microbial lipid technology for better resource efficiency and economical viability.
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the integrated production of Microbial Lipids and bio sio2 from rice husks by an organic electrolytes pretreatment technology
Bioresource Technology, 2014Co-Authors: Jing Tian, Hongwei Shen, Haibo Xie, Qian WangAbstract:Abstract In this study, a full dissolution behavior of rice husks (RHs) in ionic liquids-based organic electrolytes was achieved, and physicochemical effect of the dissolution pretreatment on the structures of RHs was elucidated. The physicochemical changes led to an enhanced subsequent enzymatic saccharification of RHs, and a total reducing sugars (TRSs) yield of 0.70 g g−1, and a glucose yield of 0.43 g g−1 were obtained. The hydrolysates could be used as carbon sources for the cultivation of Rhodosporidium toruloides Y4 for the production of Microbial Lipids with a satisfactory productivity of cell biomass (13.3 g L−1) and lipid content (32.5%) after 100 h cultivation. Further pyrolysis of the residuals after the enzymatic hydrolysis at 600 °C for 3 h resulted in new uniform, spherical silica powder materials with particle size around 150 nm, and surface area of 179.3 m2 g−1.
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efficient conversion of biomass into Lipids by using the simultaneous saccharification and enhanced lipid production process
Biotechnology for Biofuels, 2013Co-Authors: Zhiwei Gong, Hongwei Shen, Haibo Xie, Qian Wang, Xiaobing Yang, Zongbao K. ZhaoAbstract:Background Microbial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce Lipids. However, the costs of Microbial Lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of Microbial lipid technology.
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phase switching homogeneous catalysis for clean production of biodiesel and glycerol from soybean and Microbial Lipids
Chemcatchem, 2012Co-Authors: Xuefeng Cao, Hongwei Shen, Haibo Xie, Bo JingAbstract:With the increasing concerns into the utilization of Lipids as renewable resources for production of biofuels, chemicals, and materials, the efficient extraction and conversion of lipid is one of most important issues for the foundation of cost competitive lipid-economy. In this study, we found that by using a range of switchable solvents from 1,8-diazabicyclo[5.4.0]undec-7-ene to methanol, CO2, a recognized greenhouse gas, can be used as a trigger to catalyze the methanolysis of soybean and Microbial Lipids, and facilitate the separation of fatty acid methyl esters (FAMEs) and glycerol. It was found that the FAMEs can be decanted from the system in a 95.2?% conversion efficiency. The produced glycerol can be extracted from the FAMEs completely by the switchable solvents, and can be separated with high purity after recycling the DBU by an easy extraction process. Our primary investigation of the in situ extraction and conversion of Microbial Lipids from oleaginous yeast (Rhodosporidium toruloides Y4) shows that, although a 95.4?% extraction yield was achieved, the methanolysis efficiency was only 21.9?%. Our study presents an efficient and facile process for the integrated clean production of biodiesel and glycerol from soybean and Microbial Lipids, and the catalyst DBU can be recycled by an easy extraction process.
Neelamegam Annamalai - One of the best experts on this subject based on the ideXlab platform.
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enhanced production of Microbial Lipids from waste office paper by the oleaginous yeast cryptococcus curvatus
Fuel, 2018Co-Authors: Nallusamy Sivakumar, Neelamegam Annamalai, Piotr OleskowiczpopielAbstract:Abstract Waste paper has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and its abundance at low cost. In the present study, pretreated waste office paper (WOP) was enzymatically hydrolysed and used for lipid production by Cryptococcus curvatus . The results suggested that the WOP hydrolysate supplemented with ammonium sulphate (2 g/L) and yeast extract (0.5 g/L) as nitrogen source at a C/N ratio of 80 were the most suitable for high yield of Lipids. The biomass, lipid yield, lipid content and lipid coefficient achieved from batch cultivation of C. curvatus using untreated and pretreated WOP hydrolysates were 6.32 and 15.20 g/L, 1.39 and 5.75 g/L, 22 and 37.8%, and 99.9 and 234.6 mg/g sugar with the productivity of 0.02 and 0.08 g/L/h, respectively. The fatty acid profile of the Lipids indicated that the oleic acid was the major fatty acid followed by palmitic acid, stearic acid and linoleic acid which is quite similar to plant/vegetable oils. Thus, the results suggested that the waste office paper could be an alternative feedstock for production of Microbial Lipids for biodiesel.