The Experts below are selected from a list of 24438 Experts worldwide ranked by ideXlab platform
Francisco Valero - One of the best experts on this subject based on the ideXlab platform.
-
second and third generation biodiesel production with immobilised recombinant rhizopus oryzae lipase influence of the support substrate acidity and bioprocess scale up
Bioresource Technology, 2021Co-Authors: Josu Lopezfernandez, Maria Dolors Benaiges, Francisco ValeroAbstract:Abstract Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace Oil. The presence of surface hydrocarbon chains increased the operational stability of the biocatalysts supported on Purolite® and superparamagnetic particles up to 9 and 2 times, respectively. By contrast, the presence of functional groups had no effect on the initial transesterification rate, which was twice higher with the lipase immobilised onto Purolite®. Also, functionalising Purolite® with epoxide and octadecyl groups led to the highest biodiesel and volumetric productivity. This biocatalyst with other substrates including makauba, jatropha, waste cooking Oil, and Microbial Oil, led to similar initial reaction rates. However, simply raising substrate acidity from 0.5 to 2% increased the operational stability of the biocatalysts 15 times. A synergistic effect between acyl-acceptor concentration and substrate acidity was observed. The transesterification reaction was successfully scaled up to 50 mL.
-
enzymatic biodiesel synthesis from yeast Oil using immobilized recombinant rhizopus oryzae lipase
Bioresource Technology, 2015Co-Authors: Susan Hartwig Duarte, Gonzalo Hernandez, Albert Canet, Maria Dolors Benaiges, Francisco Maugeri, Francisco ValeroAbstract:The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell Oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of Microbial Oil is quite similar in terms of saponifiable lipids than olive Oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell Oil, olive Oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with Microbial Oils lower than olive Oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
Chao Huang - One of the best experts on this subject based on the ideXlab platform.
-
efficient bioconversion from acid hydrolysate of waste oleaginous yeast biomass after Microbial Oil extraction to bacterial cellulose by komagataeibacter xylinus
Preparative Biochemistry & Biotechnology, 2017Co-Authors: Chao Huang, Gaoxiang Qi, Lian Xiong, Xuefang Chen, Qianlin Huang, Lanlan Tian, Hailong Li, Xinde ChenAbstract:Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after Microbial Oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also ...
-
semi pilot scale Microbial Oil production by trichosporon cutaneum using medium containing corncob acid hydrolysate
Applied Biochemistry and Biotechnology, 2016Co-Authors: Chao Huang, Lian Xiong, Xuefang Chen, Can Wang, Xiaoqing Lin, Silan Shi, Dan Yang, Xinde ChenAbstract:In this study, semi-pilot scale Microbial Oil production by Trichosporon cutaneum using medium containing corncob acid hydrolysate was carried out in a 50-L fermentor. Scale up showed no negative influence on lipid fermentation that no obvious lag phase was observed. Both glucose and xylose could be utilized simultaneously by T. cutaneum, but the utilization rate of xylose was much slower than that of glucose. After 7.6 days of fermentation, the biomass, lipid content, and lipid yield were 21.8 g/L, 53.7 %, and 11.7 g/L, respectively. Also, a high lipid coefficient (lipid yield on sugars consumption) of 26.3 was obtained. Besides Microbial Oil, polysaccharide was another main product of lipid fermentation that the remaining biomass residue full of polysaccharides after lipid extraction could be one important by-product in future. Overall, this study showed the great potential of industrialization for lipid production by T. cutaneum on low-cost substrates especially for lignocellulosic hydrolysates.
-
use of elephant grass pennisetum purpureum acid hydrolysate for Microbial Oil production by trichosporon cutaneum
Preparative Biochemistry & Biotechnology, 2016Co-Authors: Xuefang Chen, Gaoxiang Qi, Lian Xiong, Chao Huang, Bo Wang, Can Wang, Xinde ChenAbstract:ABSTRACTElephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant grass acid hydrolysate was investigated for the first time. During the fermentation process on the elephant grass acid hydrolysate, glucose, xylose, and arabinose could be well utilized as carbon sources by T. cutaneum. Interestingly, xylose was almost no use before glucose was consumed completely. This illustrated that simultaneous saccharification of xylose and glucose by T. cutaneum did not occur on elephant grass acid hydrolysate. The highest biomass, lipid content, lipid yield, and lipid coefficient of T. cutaneum were measured after the sixth day of fermentation and were 22.76 g/L, 24.0%, 5.46 g/L, and 16.1%, respectively. Therefore, elephant grass is a promising raw material for Microbial Oil production by T. cutaneum.
-
effects of acetic acid on growth and lipid production by cryptococcus albidus
Journal of the American Oil Chemists' Society, 2015Co-Authors: Xiaomei Li, Lian Xiong, Chao Huang, Xuefang Chen, Xinde ChenAbstract:The cell growth and lipid accumulation process of Cryptococcus albidus were investigated using acetic acid as the sole carbon source at different concentrations. C. albidus showed high tolerance to acetic acid at a high concentration of 30 g L−1. The highest lipid content (32.69 ± 0.50 %) and lipid yield (0.96 ± 0.05 g L−1) were both obtained in the medium with an initial acetic acid concentration of 30 g L−1 on day five. Interestingly, the maximum lipid content and lipid yield was obtained on a different day in a medium with different acetic acid concentration. The fatty acid composition of the lipids accumulated by C. albidus was 16–23 % palmitic acid (C16:0), 3–5 % linolenic acid (C18:3), 42–51 % linoleic acid (C18:2) and 23–27 % oleic acid (C18:1), which was similar to that of soybean Oil; thus, this Microbial Oil has great potential value as a renewable biodiesel feedstock. This work also provides valuable information for further research to use cheap substrates containing a high concentration of acetic acid (such as lignocellulosic hydrolysates), which is an economical and environmentally friendly form of Microbial Oil production.
-
kinetics and mechanism analysis on Microbial Oil production by trichosporon fermentans in rice straw hydrolysate
Industrial & Engineering Chemistry Research, 2014Co-Authors: Chao Huang, Minhua Zong, Liping Liu, Wenyong LouAbstract:To give an insight into the kinetics of Microbial Oil fermentation with lignocellulosic hydrolysate as substrate, lipid production by Trichosporon fermentans in rice straw hydrolysate was investigated in a 5 L fermentor. For comparison, fermentation in its simulated medium was also performed to evaluate the effect of inhibitors present in the rice straw hydrolysate on the cell growth and lipid accumulation of T. fermentans. The optimum fermentation time, maximum biomass, and lipid content of T. fermentans in the rice straw hydrolysate were 10.5 days, 28.6 g/L, and 43.9%, respectively, while the corresponding values in the simulated medium were 8.5 days, 27.0 g/L, and 65.0%, respectively, indicating that the inhibitors in the rice straw hydrolysate did show some inhibition on the cell growth and lipid accumulation of T. fermentans. Kinetic models of lipid fermentation with T. fermentans showed the maximum specific growth rate and the correlation coefficient of lipid synthesis to cell growth in the rice straw hydrolysate were 0.40 and 0.51, respectively, which are much lower than those in the simulated medium (0.58 and 0.73). To better understand the influential mechanism of inhibitors in the rice straw hydrolysate on the growth and lipid accumulation of T. fermentans, the physiological and biochemical changes of cells in the fermentation were further investigated. The reduced activities of adenosine triphosphate (ATP) citrate lyase, malic enzyme, and xylose reductase, and the elongation of cells at the beginning of fermentation, partly account for the inhibitory effect of inhibitors in the rice straw hydrolysate.
Maria Dolors Benaiges - One of the best experts on this subject based on the ideXlab platform.
-
second and third generation biodiesel production with immobilised recombinant rhizopus oryzae lipase influence of the support substrate acidity and bioprocess scale up
Bioresource Technology, 2021Co-Authors: Josu Lopezfernandez, Maria Dolors Benaiges, Francisco ValeroAbstract:Abstract Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace Oil. The presence of surface hydrocarbon chains increased the operational stability of the biocatalysts supported on Purolite® and superparamagnetic particles up to 9 and 2 times, respectively. By contrast, the presence of functional groups had no effect on the initial transesterification rate, which was twice higher with the lipase immobilised onto Purolite®. Also, functionalising Purolite® with epoxide and octadecyl groups led to the highest biodiesel and volumetric productivity. This biocatalyst with other substrates including makauba, jatropha, waste cooking Oil, and Microbial Oil, led to similar initial reaction rates. However, simply raising substrate acidity from 0.5 to 2% increased the operational stability of the biocatalysts 15 times. A synergistic effect between acyl-acceptor concentration and substrate acidity was observed. The transesterification reaction was successfully scaled up to 50 mL.
-
enzymatic biodiesel synthesis from yeast Oil using immobilized recombinant rhizopus oryzae lipase
Bioresource Technology, 2015Co-Authors: Susan Hartwig Duarte, Gonzalo Hernandez, Albert Canet, Maria Dolors Benaiges, Francisco Maugeri, Francisco ValeroAbstract:The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell Oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of Microbial Oil is quite similar in terms of saponifiable lipids than olive Oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell Oil, olive Oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with Microbial Oils lower than olive Oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
Everson Alves Miranda - One of the best experts on this subject based on the ideXlab platform.
-
rhodotorula toruloides single cell Oil production using eucalyptus urograndis hemicellulose hydrolysate as a carbon source
Energies, 2020Co-Authors: Helberth Junnior Santos Lopes, Nemailla Bonturi, Everson Alves MirandaAbstract:Microbial Oil is a potential substitute for vegetable Oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for Microbial Oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.
-
Microbial Oil production in sugarcane bagasse hemicellulosic hydrolysate without nutrient supplementation by a rhodosporidium toruloides adapted strain
Process Biochemistry, 2017Co-Authors: Nemailla Bonturi, Aline Crucello, Americo Jose Carvalho Viana, Everson Alves MirandaAbstract:Abstract Sugarcane bagasse hemicellulosic hydrolysate (SCBH) is a low-cost substrate for single-cell Oil (SCO) production. However, this hydrolysate has an undesirable low carbon/nitrogen (C/N) ratio and contains inhibitors. Yeast adaptation is a simple strategy to overcome the presence of inhibitors, while the concentration of the hydrolysate and glycerol supplementation are alternatives to solve the low C/N limitation. This work aimed to investigate the adaptation of the yeast Rhodosporidium toruloides in undetoxified SCBH and its use in SCO production. The adapted strain produced more lipids than the parental strain: the concentration of SCBH or the addition of glycerol increased the lipid content, concentration, and productivity to at least 108%, 175%, and 118%, respectively. Lipid production in SCBH was done without salts or nitrogen supplementation. Transcriptomic study showed that hydrolysate-tolerance- and lipid accumulation-related genes were strongly induced. These genes can be targets for metabolic engineering.
-
high concentrations of dried sorghum stalks as a biomass feedstock for single cell Oil production by rhodosporidium toruloides
Biotechnology for Biofuels, 2015Co-Authors: Leonidas Matsakas, Ulrika Rova, Nemailla Bonturi, Everson Alves Miranda, Paul ChristakopoulosAbstract:Background Environmental crisis and concerns for energy security have made the research for renewable fuels that will substitute the usage of fossil fuels an important priority. Biodiesel is a potential substitute for petroleum, but its feasibility is hindered by the utilization of edible vegetable Oil as raw material, which is responsible for a large fraction of the production cost and fosters the food versus fuel competition. Microbial Oils are an interesting alternative as they do not compete with food production, and low cost renewable materials could serve as raw materials during cultivation of microorganisms. Sweet sorghum is an excellent candidate as substrate for Microbial Oil production, as it possesses high photosynthetic activity yielding high amounts of soluble and insoluble carbohydrates, and does not require high fertilization and irrigation rates.
Xinde Chen - One of the best experts on this subject based on the ideXlab platform.
-
efficient bioconversion from acid hydrolysate of waste oleaginous yeast biomass after Microbial Oil extraction to bacterial cellulose by komagataeibacter xylinus
Preparative Biochemistry & Biotechnology, 2017Co-Authors: Chao Huang, Gaoxiang Qi, Lian Xiong, Xuefang Chen, Qianlin Huang, Lanlan Tian, Hailong Li, Xinde ChenAbstract:Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after Microbial Oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also ...
-
semi pilot scale Microbial Oil production by trichosporon cutaneum using medium containing corncob acid hydrolysate
Applied Biochemistry and Biotechnology, 2016Co-Authors: Chao Huang, Lian Xiong, Xuefang Chen, Can Wang, Xiaoqing Lin, Silan Shi, Dan Yang, Xinde ChenAbstract:In this study, semi-pilot scale Microbial Oil production by Trichosporon cutaneum using medium containing corncob acid hydrolysate was carried out in a 50-L fermentor. Scale up showed no negative influence on lipid fermentation that no obvious lag phase was observed. Both glucose and xylose could be utilized simultaneously by T. cutaneum, but the utilization rate of xylose was much slower than that of glucose. After 7.6 days of fermentation, the biomass, lipid content, and lipid yield were 21.8 g/L, 53.7 %, and 11.7 g/L, respectively. Also, a high lipid coefficient (lipid yield on sugars consumption) of 26.3 was obtained. Besides Microbial Oil, polysaccharide was another main product of lipid fermentation that the remaining biomass residue full of polysaccharides after lipid extraction could be one important by-product in future. Overall, this study showed the great potential of industrialization for lipid production by T. cutaneum on low-cost substrates especially for lignocellulosic hydrolysates.
-
use of elephant grass pennisetum purpureum acid hydrolysate for Microbial Oil production by trichosporon cutaneum
Preparative Biochemistry & Biotechnology, 2016Co-Authors: Xuefang Chen, Gaoxiang Qi, Lian Xiong, Chao Huang, Bo Wang, Can Wang, Xinde ChenAbstract:ABSTRACTElephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant grass acid hydrolysate was investigated for the first time. During the fermentation process on the elephant grass acid hydrolysate, glucose, xylose, and arabinose could be well utilized as carbon sources by T. cutaneum. Interestingly, xylose was almost no use before glucose was consumed completely. This illustrated that simultaneous saccharification of xylose and glucose by T. cutaneum did not occur on elephant grass acid hydrolysate. The highest biomass, lipid content, lipid yield, and lipid coefficient of T. cutaneum were measured after the sixth day of fermentation and were 22.76 g/L, 24.0%, 5.46 g/L, and 16.1%, respectively. Therefore, elephant grass is a promising raw material for Microbial Oil production by T. cutaneum.
-
effects of acetic acid on growth and lipid production by cryptococcus albidus
Journal of the American Oil Chemists' Society, 2015Co-Authors: Xiaomei Li, Lian Xiong, Chao Huang, Xuefang Chen, Xinde ChenAbstract:The cell growth and lipid accumulation process of Cryptococcus albidus were investigated using acetic acid as the sole carbon source at different concentrations. C. albidus showed high tolerance to acetic acid at a high concentration of 30 g L−1. The highest lipid content (32.69 ± 0.50 %) and lipid yield (0.96 ± 0.05 g L−1) were both obtained in the medium with an initial acetic acid concentration of 30 g L−1 on day five. Interestingly, the maximum lipid content and lipid yield was obtained on a different day in a medium with different acetic acid concentration. The fatty acid composition of the lipids accumulated by C. albidus was 16–23 % palmitic acid (C16:0), 3–5 % linolenic acid (C18:3), 42–51 % linoleic acid (C18:2) and 23–27 % oleic acid (C18:1), which was similar to that of soybean Oil; thus, this Microbial Oil has great potential value as a renewable biodiesel feedstock. This work also provides valuable information for further research to use cheap substrates containing a high concentration of acetic acid (such as lignocellulosic hydrolysates), which is an economical and environmentally friendly form of Microbial Oil production.
-
Bioconversion of Corncob Acid Hydrolysate into Microbial Oil by the Oleaginous Yeast Lipomyces starkeyi
Applied biochemistry and biotechnology, 2013Co-Authors: Chao Huang, Lian Xiong, Bo Wang, Chen Xuefang, Xiaoyan Yang, Lin Xiaoqing, Juan Yang, Xinde ChenAbstract:For the first time, corncob acid hydrolysate was used for Microbial Oil production by the oleaginous yeast Lipomyces starkeyi. After hydrolysis by dilute sulfuric acid, corncob could turn into an acid hydrolysate with a sugar concentration of about 42.3 g/L. Detoxified by overliming and absorption with activated carbon, the corncob hydrolysate could be used by L. starkeyi efficiently that a total biomass of 17.2 g/L with a lipid content of 47.0 % (corresponding to a lipid yield of 8.1 g/L) and a lipid coefficient of 20.9 could be obtained after cultivation on the corncob hydrolysate for 8 days. Therefore, L. starkeyi is a promising strain for Microbial Oil production from lignocellulosic biomass. Glucose and xylose were used by L. starkeyi simultaneously during lipid fermentation while arabinose could not be utilized by it. Besides, the lipid composition of L. starkeyi was similar to that of vegetable Oils; thus, it is a promising feedstock for biodiesel production.