The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform

Romney M Humphries - One of the best experts on this subject based on the ideXlab platform.

E. Deak - One of the best experts on this subject based on the ideXlab platform.

  • Microbiology Test Reliability in Differentiation of Neisseria meningitidis and Neisseria polysaccharea
    2016
    Co-Authors: E. Deak, A N. Green, B R. M. Humphriesa
    Abstract:

    We read with interest the report by Cunningham and col-leagues (1) regarding the inability of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to differentiate Neisseria meningitidis from Neisseria polysaccharea. As highlighted in the article, these two neisseriae are also difficult to distinguish by biochemical methods. Key reactions that might be used include assessing prolyl aminopeptidase, the ability to use sucrose to produce amylopectin, and the presence of -glutamyl aminopeptidase (GGT); however, neither of these Tests is 100 % sensitive or specific (2, 3). The importance of the similarity between these two organisms was recently reinforced at our institution, when a Neisseria sp. strain isolated from the blood and cerebrospinal fluid (CSF) of a 54-year-old woman who pre-sented with meningitis symptoms was referred to our laboratory for susceptibility Testing from an outside hospital (OSH). The blood isolate was identified by the OSH laboratory as N. polysac

  • Microbiology Test reliability in differentiation of neisseria meningitidis and neisseria polysaccharea
    Journal of Clinical Microbiology, 2014
    Co-Authors: E. Deak, N Green, Romney M Humphries
    Abstract:

    We read with interest the report by Cunningham and colleagues ([1][1]) regarding the inability of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to differentiate Neisseria meningitidis from Neisseria polysaccharea. As highlighted in the article, these

N Green - One of the best experts on this subject based on the ideXlab platform.

Ingrid Smith - One of the best experts on this subject based on the ideXlab platform.

  • Use of Microbiology Tests in the era of increasing AMR rates– a multicentre hospital cohort study
    Antimicrobial resistance and infection control, 2019
    Co-Authors: Brita Skodvin, Jannicke Slettli Wathne, P. Christoffer Lindemann, Stig Harthug, Roy Miodini Nilsen, Esmita Charani, Heidi Syre, Baard R. Kittang, Lars Kåre Selland Kleppe, Ingrid Smith
    Abstract:

    Effective use of Microbiology Test results may positively influence patient outcomes and limit the use of broad-spectrum antibiotics. However, studies indicate that their potential is not fully utilized. We investigated Microbiology Test ordering practices and the use of Test results for antibiotic decision-making in hospitals. A multicentre cohort study was conducted during five months in 2014 in Medical departments across three hospitals in Western Norway. Patients treated with antibiotics for sepsis, urinary tract infections, skin and soft tissue infections, lower respiratory tract infections or acute exacerbations of chronic obstructive pulmonary disease were included in the analysis. Primary outcome measures were degree of Microbiology Test ordering, compliance with Microbiology Testing recommendations in the national antibiotic guideline and proportion of Microbiology Test results used to inform antibiotic treatment. Data was obtained from electronic- and paper medical records and charts and laboratory information systems. Of the 1731 patient admissions during the study period, mean compliance with Microbiology Testing recommendations in the antibiotic guideline was 89%, ranging from 81% in patients with acute exacerbations of chronic obstructive pulmonary disease to 95% in patients with sepsis. Substantial additional Testing was performed beyond the recommendations with 298/606 (49%) of patients with lower respiratory tract infections having urine cultures and 42/194 (22%) of patients with urinary tract infections having respiratory Tests. Microbiology Test results from one of the hospitals showed that 18% (120/672) of patient admissions had applicable Test results, but only half of them were used for therapy guidance, i.e. in total, 9% (63/672) of patient admissions had Test results informing prescription of antibiotic therapy. This study showed that despite a large number of Microbiology Test orders, only a limited number of Tests informed antibiotic treatment. To ensure that Microbiology Tests are used optimally, there is a need to review the utility of existing Microbiology Tests, Test ordering practices and use of Test results through a more targeted and overarching approach.

Stephen A Schaub - One of the best experts on this subject based on the ideXlab platform.

  • technology assessment and strategy for development of a rapid field water Microbiology Test kit
    1991
    Co-Authors: David R Preston, Stephen A Schaub
    Abstract:

    Abstract : A literature and market search of existing technology for the detection, identification, and quantification of microorganisms in water was conducted. Based upon the availability of technologies and their configurations, an assessment of the appropriate strategies to pursue for the near and long term development plans in development of the Rapid Field Bacteriology Test Kit was performed. Near term technologies to improve the Army's capability to detect microorganisms would appear to be essentially improvements in versatility and measurement of coliform indicator organisms. New chromogenic and fluorogenic indicator substances associated with new substrates appear to be best suited for Test kit development either for quantitative membrane filter Tests or presence/ absence and multiple fermentation Tests. Test times, incubator requirements, and operator involvement appear to be similar to older technologies. Long term development would appear to favor such technologies as genetic probes with amplification of the hydridized nucleic acid materials of positive samples, and some immunological based systems such as enzyme linked, immuno-sorbent assays. In both cases, the major problems would appear to be sample preparation and development of signal strengths from the reactions which would allow the user to see results in 1 hour.

  • technology assessment and strategy for development of a rapid field water Microbiology Test kit technical report
    1991
    Co-Authors: David R Preston, Stephen A Schaub
    Abstract:

    A literature and market search of existing technology for the detection, identification, and quantification of microorganisms in water was conducted. Based upon the availability of technologies and their configurations, an assessment of the appropriate strategies to pursue for the near and long term development plans in development of the Rapid Field Bacteriology Test Kit was performed. Near term technologies to improve the Army's capability to detect microorganisms would appear to be essentially improvements in versatility and measurement of coliform indicator organisms. New chromogenic and fluorogenic indicator substances associated with new substrates appear to be best suited for Test kit development either for quantitative membrane filter Tests or presence/absence and multiple fermentation Tests. Test times, incubator requirements, and operator involvement appear to be similar to older technologies. Long term development would appear to favor such technologies as genetic probes with amplification of the hydridized nucleic acid materials of positive samples, and some immunological based systems such as enzyme linked, immuno-sorbent assays. In both cases, the major problems would appear to be sample preparation and development of signal strengths from the reactions which would allow the user to see results in 1 hour.