The Experts below are selected from a list of 53253 Experts worldwide ranked by ideXlab platform
Grover Ak - One of the best experts on this subject based on the ideXlab platform.
-
Serum beta-2 Microglobulin and C-reactive protein levels in acute adenovirus conjunctivits
Acta Ophthalmologica, 2009Co-Authors: Ak Gupta, M. D. Mathur, G. S. Sarin, Grover AkAbstract:Serum beta-2 Microglobulin and C-reactive protein levels were measured in 52 patients with acute adenovirus conjunctivitis and 58 healthy subjects, by ELISA technique. Serum beta-2 Microglobulin levels were found to be significantly increased while there was no change in C-reactive protein levels. Serum beta-2 Microglobulin levels closely paralleled the severity of the disease. The increased beta-2 Microglobulin level in serum showed a significant fall to normal level on clinical improvement. There was no difference in the serum beta-2 Microglobulin levels in virus-isolation positive and negative patients.
-
Serum beta‐2 Microglobulin and C‐reactive protein levels in acute adenovirus conjunctivits
Acta Ophthalmologica, 2009Co-Authors: Ak Gupta, M. D. Mathur, G. S. Sarin, Grover AkAbstract:: Serum beta-2 Microglobulin and C-reactive protein levels were measured in 52 patients with acute adenovirus conjunctivitis and 58 healthy subjects, by ELISA technique. Serum beta-2 Microglobulin levels were found to be significantly increased while there was no change in C-reactive protein levels. Serum beta-2 Microglobulin levels closely paralleled the severity of the disease. The increased beta-2 Microglobulin level in serum showed a significant fall to normal level on clinical improvement. There was no difference in the serum beta-2 Microglobulin levels in virus-isolation positive and negative patients.
Ak Gupta - One of the best experts on this subject based on the ideXlab platform.
-
Serum beta-2 Microglobulin and C-reactive protein levels in acute adenovirus conjunctivits
Acta Ophthalmologica, 2009Co-Authors: Ak Gupta, M. D. Mathur, G. S. Sarin, Grover AkAbstract:Serum beta-2 Microglobulin and C-reactive protein levels were measured in 52 patients with acute adenovirus conjunctivitis and 58 healthy subjects, by ELISA technique. Serum beta-2 Microglobulin levels were found to be significantly increased while there was no change in C-reactive protein levels. Serum beta-2 Microglobulin levels closely paralleled the severity of the disease. The increased beta-2 Microglobulin level in serum showed a significant fall to normal level on clinical improvement. There was no difference in the serum beta-2 Microglobulin levels in virus-isolation positive and negative patients.
-
Serum beta‐2 Microglobulin and C‐reactive protein levels in acute adenovirus conjunctivits
Acta Ophthalmologica, 2009Co-Authors: Ak Gupta, M. D. Mathur, G. S. Sarin, Grover AkAbstract:: Serum beta-2 Microglobulin and C-reactive protein levels were measured in 52 patients with acute adenovirus conjunctivitis and 58 healthy subjects, by ELISA technique. Serum beta-2 Microglobulin levels were found to be significantly increased while there was no change in C-reactive protein levels. Serum beta-2 Microglobulin levels closely paralleled the severity of the disease. The increased beta-2 Microglobulin level in serum showed a significant fall to normal level on clinical improvement. There was no difference in the serum beta-2 Microglobulin levels in virus-isolation positive and negative patients.
Alfred Bernard - One of the best experts on this subject based on the ideXlab platform.
-
potential role of clara cell protein an endogenous phospholipase a2 inhibitor in acute lung injury
European Respiratory Journal, 1995Co-Authors: Philippe G. Jorens, Wout De Backer, Frans J. Van Overveld, Yves Sibille, Robert Lauwerys, Roderick J. Flower, N. J. Goulding, Leo Bossaert, A. G. Herman, Alfred BernardAbstract:It is now recognized that epithelial cells lining airways and alveoli are capable of releasing various mediators, which have the potential to modulate local inflammatory reactions. The amount of the 16 kDa Clara cell protein (CC16), an inhibitor of phospholipase A2 activity produced by pulmonary epithelial cells, was measured by means of a sensitive immunoassay in the unconcentrated bronchoalveolar lavage fluid (BALF) of 13 control subjects, and in patients with acute lung injury (14 with the full-blown adult respiratory distress syndrome (ARDS); 21 after standard cardiopulmonary bypass surgery, a known risk factor for ARDS). The level of CC16 was compared with other markers of inflammation with a wide range of molecular weights: albumin (nephelometry); total protein (spectrophotometry); beta 2-Microglobulin (latex immunoassay); cystatin C (latex immunoassay); alpha 1-antitrypsin (immunoradiometry), and lipocortin-1 (enzyme-linked immunosorbent assay (ELISA)). The Clara cell protein (CC16) was detectable in all BALF, and significantly higher levels of this protein were observed in BALF from patients with acute lung injury. Changes in BALF Clara cell protein levels differed from those of alpha 2-macroglobulin and the natural phospholipase inhibitor lipocortin-1. Alpha 2-macroglobulin levels were not significantly enhanced in patients at risk for ARDS, but were increased in patients with ARDS; whereas, lipocortin 1 levels were not elevated in either group. Pretreatment of patients at risk for ARDS with high dose methylprednisolone did not alter the amount of Clara cell protein recovered in BALF. The mean CC16 level in BALF from patients with ARDS who died was significantly lower than from those who survived. The data presented in this study suggest that pulmonary epithelial cells secrete a natural anti-inflammatory protein during acute lung injury, which might have a protective and immunosuppressive role.
-
Charge-dependent renal uptake of beta 2-Microglobulin in conscious rats.
Scandinavian Journal of Clinical & Laboratory Investigation, 1992Co-Authors: Alfred Bernard, A Ouled Amor, Robert LauwerysAbstract:The influence of molecular charge on the tubular reabsorption of proteins was studied in conscious rats injected intravenously with beta 2-Microglobulins of different isoelectric points (pI). Native human beta 2-Microglobulin (pI 5.8), two anionized (pI 4.85 and 5.55) and three cationized derivatives (pI 7.2, 8.35 and 8.7) were used. The six forms of beta 2-Microglobulin had a molecular radius between 15.7 and 15.9 A. The renal uptake was estimated by measuring the amount excreted in urine with a sensitive immunoassay. The ability of rat kidney to reabsorb beta 2-Microglobulin was clearly related to the net charge of the protein. Increasing the pI of the protein significantly reduced the urinary excretion, whereas lowering it had the opposite effect. Anionization was particularly effective in reducing the beta 2-Microglobulin uptake, since a decrease of the pI of one unit enhanced the urinary output by two orders of magnitude. This charge-dependency persisted when the tubular reabsorption of proteins was partly inhibited by lysozyme. By contrast, it was practically abolished by lysine, probably because the inhibitory effect of this amino acid on protein tubular reabsorption is not competitive. The administration of ammonium chloride in rats produced an immediate and transient elevation of rat beta 2-Microglobulinuria. This phenomenon, which was partly inhibited by the subsequent administration of sodium bicarbonate, presumably results from a competition between the NH4+ ion and beta 2-Microglobulin for tubular binding sites. These data support the hypothesis that proteins bind to the luminal membrane of tubular cells mainly via positively charged amino groups.
Bo Åkerström - One of the best experts on this subject based on the ideXlab platform.
-
Heme-Scavenging Role of α1-Microglobulin in Chronic Ulcers
The Journal of investigative dermatology, 2003Co-Authors: Maria Allhorn, Katarina Lundqvist, Artur Schmidtchen, Bo ÅkerströmAbstract:Chronic venous ulcers are characterized by chronic inflammation. Heme and iron, originating from blood cell hemolysis as well as extravascular necrosis, have been implicated as important pathogenic factors due to their promotion of oxidative stress. It was recently reported that the plasma and tissue protein α 1 -Microglobulin is involved in heme metabolism. The protein binds heme, and a carboxy-terminally processed form, truncated α 1 -Microglobulin, also degrades heme. Here, we show the presence of micromolar levels of heme and free iron in chronic leg ulcer fluids. Micromolar amounts of α 1 -Microglobulin was also present in the ulcer fluids and bound to added radiolabeled heme. Truncated α 1 -Microglobulin was found in the ulcer fluids and exogenously added α 1 -Microglobulin was processed into the truncated α 1 -Microglobulin form. Histochemical analysis of chronic wound tissue showed the presence of iron deposits, heme/porphyrins in infiltrating cells basement membranes and fibrin cuffs around vessels, and α 1 -Microglobulin ubiquitously distributed but especially abundant in basement membranes around vessels and at fibrin cuffs. Our results suggest that α 1 -Microglobulin constitutes a previously unknown defense mechanism against high heme and iron levels during skin wound healing. Excessive heme and iron, which are not buffered by α 1 -Microglobulin, may underlie the chronic inflammation in chronic ulcers.
-
Isolation of plaice (Pleuronectes platessa) α1-Microglobulin: conservation of structure and chromophore
Biochimica et Biophysica Acta, 1999Co-Authors: Annika Lindqvist, Bo ÅkerströmAbstract:A cDNA coding for plaice (Pleuronectes platessa) α1-Microglobulin (Leaver et al., 1994, Comp. Biochem. Physiol. 108B, 275-281) was expressed and purified from baculovirus-infected insect cells. Specific monoclonal antibodies were then prepared and used to isolate the protein from plaice liver and serum. Mature 28.5 kDa α1-Microglobulin was found in both liver and serum. The protein consisted of an 184 amino acid peptide with a complex N-glycan in position Asn123, one intrachain disulfide bridge and a yellow-brown chromophore. Physicochemical characterization indicated a globular shape with a frictional ratio of 1.37, electrophoretic charge-heterogeneity and antiparallel β-sheet structure. A smaller, incompletely glycosylated, yellow-brown α1-Microglobulin as well as a 45 kDa precursor protein were also found in liver. The chromophore was found to be linked to α1-Microglobulin intracellularly. Recombinant plaice α1-Microglobulin isolated from insect cells had the same N-terminal sequence, globular shape and yellow-brown color as mature α1-Microglobulin, but carried a smaller, fucosylated, non-sialylated N-glycan in the Asn123 position. The concentration of α1-Microglobulin in plaice serum was 20 mg/l and it was found both as a 28.5 kDa component and as high molecular weight components. Thus, the size, shape, charge and color of plaice α1-Microglobulin were similar to mammalian α1-Microglobulin, indicating a high degree of structural conservation between fish and human α1-Microglobulin. The monoclonal antibodies against plaice α1-Microglobulin cross-reacted with human α1-Microglobulin, emphasizing the structural similarity. Copyright (C) 1999 Elsevier Science B.V. (Less)
-
Prothrombin, albumin and immunoglobulin A form covalent complexes with alpha1-Microglobulin in human plasma
European journal of biochemistry, 1997Co-Authors: Tord Berggård, Nils Thelin, Cecilia Falkenberg, Jan J. Enghild, Bo ÅkerströmAbstract:Molecules containing the 33-kDa plasma protein alpha1-Microglobulin were isolated from human plasma by anti-(alpha1-Microglobulin) affinity chromatography. Five major bands could be seen after electrophoretic separation of the alpha1-Microglobulin-containing proteins under native conditions. Immunoblotting demonstrated alpha1-Microglobulin in all five bands. Two of these have been described previously: free alpha1-Microglobulin and alpha1-Microglobulin complexed with IgA (IgA x alpha1-Microglobulin). The other three bands were identified as prothrombin alpha1-Microglobulin, albumin x alpha1-Microglobulin and dimeric alpha1-Microglobulin. Prothrombin x alpha1-Microglobulin were 1:2 and 1:1 complexes which carried approximately 1% of total alpha1-Microglobulin, had molecular masses of about 145 kDa and 110 kDa upon SDS/PAGE and dissociated completely to free alpha1-Microglobulin and prothrombin (72 kDa) when reducing agents were added, suggesting that the complexes were stabilized by disulfide bonds. The alpha1-Microglobulin molecules did not inhibit cleavage of prothrombin by factor Xa and were bound to the peptides which were released upon activation of prothrombin. Albumin x alpha1-Microglobulin, corresponding to 7% of total plasma alpha1-Microglobulin, was a mixture between 1:1 and 1:2 complexes, with masses upon SDS/PAGE of approximately 100 kDa and 135 kDa, respectively. Both these complexes dissociated only partially to free alpha1-Microglobulin and albumin when reducing agents were added. The albumin x alpha1-Microglobulin complexes carried a yellow-brown chromophore similar to free alpha1-Microglobulin. The complex-binding to alpha1-Microglobulin did not block the fatty-acid-binding ability of albumin. The plasma concentrations of albumin x alpha1-Microglobulin and prothrombin x alpha1-Microglobulin were estimated to 5.2 mg/l and 1.1 mg/l, respectively.
-
Expression of rat alpha 1-Microglobulin-bikunin in baculovirus-transformed insect cells
Protein expression and purification, 1995Co-Authors: T. Bratt, Bo ÅkerströmAbstract:cDNA encoding rat alpha 1-Microglobulin-bikunin was ligated into the transfer vector pVL 1392 and recombined with a wild-type baculovirus. The resulting alpha 1-Microglobulin-bikunin-encoding baculovirus was used to infect Trichoplusia ni (Hi-5) insect cells. The infected cells secreted alpha 1-Microglobulin with maximal concentrations of 15 mg/liter 5 days after infection. The secreted proteins migrated upon SDS-PAGE as two major protein bands, 40 and 26 kDa, corresponding to alpha 1-Microglobulin-bikunin and free alpha 1-Microglobulin. The results suggested that the cells secreted mostly alpha 1-Microglobulin-bikunin, which subsequently was cleaved in the medium, yielding free alpha 1-Microglobulin. Both forms were isolated by monoclonal anti-alpha 1-Microglobulin affinity chromatography, and alpha 1-Microglobulin-bikunin separated from free alpha 1-Microglobulin by gel chromatography. The yields of purified alpha 1-Microglobulin-bikunin and free alpha 1-Microglobulin were approximately 1 and 5 mg, respectively, per liter medium. Insect cell alpha 1-Microglobulin displayed a size, shape, and charge heterogeneity similar to alpha 1-Microglobulin isolated from rat urine. A panel of monoclonal antibodies raised against urinary alpha 1-Microglobulin from several different species bound to rat urinary alpha 1-Microglobulin and insect cell secreted alpha 1-Microglobulin-bikunin and free alpha 1-Microglobulin with approximately the same strength, indicating that the three proteins are folded in similar ways. The results of glycosidase treatments and lectin blotting indicate the absence of neuraminic acid but the presence of one N-linked oligosaccharide and an unspecified number of O-linked oligosaccharides in alpha 1-Microglobulin-bikunin and free alpha 1-Microglobulin.
Sumio Kawata - One of the best experts on this subject based on the ideXlab platform.
-
In a non-diabetic Japanese population, the combination of macroalbuminuria and increased urine beta 2-Microglobulin predicts a decline of renal function: the Takahata study
Nephrology Dialysis Transplantation, 2008Co-Authors: Ami Ikeda, Tsuneo Konta, Satoshi Takasaki, Zhimei Hao, Kazuko Suzuki, Hitoshi Sato, Yoko Shibata, Yasuchika Takeishi, Takeo Kato, Sumio KawataAbstract:Background. Glomerular and tubular damage are important factors in the development of renal insufficiency. However, the interaction of these factors is largely unknown in the non-diabetic Japanese population. To clarify the relationship between renal insufficiency and both glomerular and tubular damage, we conducted a community-based study using albuminuria and urine beta 2-Microglobulin as markers of glomerular and tubular damages, respectively. Methods. Subjects of this study were 2816 non-diabetic individuals >40 years old in Takahata, Japan. The urine albumin–creatinine ratio (UACR) and urine beta 2Microglobulin–creatinineratio(UBCR)wereassessedfrom single spot urine. The glomerular filtration rate (eGFR) was estimated using the abbreviated MDRD equation with a Japanese coefficient. Results. The prevalence of albuminuria (UACR >20 mg/ g in men and >30 mg/g in women), increased UBCR (>300 µg/g) and renal insufficiency (eGFR 200 mg/g in men and >300 mg/g in women) and increased UBCR. No urinary abnormalities were observed in 71.7% of the 611 subjects with renal insufficiency, and were more common in young, women and the non-hypertensive population. The 1-year decline of eGFR was greatest in subjects with an overlap of macroalbuminuria and increased UBCR. Conclusions. This study indicated that only a small part of renalinsufficiencyaccompaniedincreasedurinealbuminor beta 2-Microglobulin in the non-diabetic Japanese popula
