The Experts below are selected from a list of 141 Experts worldwide ranked by ideXlab platform
Jan L. Ruben - One of the best experts on this subject based on the ideXlab platform.
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Transversal wavelength-independent Microradiography, a method for monitoring caries lesions over time, validated with transversal Microradiography.
Caries research, 2006Co-Authors: R.z. Thomas, Jan L. Ruben, J. De Vries, J.j. Ten Bosch, Marie-charlotte D.n.j.m. HuysmansAbstract:This paper describes a microradiographic method for measuring mineral concentration in a transversal geometry with thick (≤3.2 mm) sections: transversal wavelength-independent Microradiography (T-WIM). It was tested on bovine enamel and dentin samples in vitro, and the results were validated with those of transversal Microradiography (TMR). 48 enamel and 48 dentin samples (3.2 × 3.2 × 1.5 mm) were embedded in acrylic resin, randomly divided into six groups of 8 dentin or 8 enamel samples, and demineralized for 0 (sound control), 1, 2, 3, 4, or 5 weeks. For T-WIM, samples were imaged on film with polychromatic 40-kV Cu X-rays with an Al (0.25 mm)/Ni (0.02 mm) filter together with an aluminium/zinc step wedge. TMR slices (about 80 µm for enamel and about 130 µm for dentine) were subsequently cut from the centre of the samples and subjected to TMR. Microradiographs from both methods were digitized and image analysis software was used to calculate lesion depth and mineral loss. The relations between T-WIM and TMR results for mineral loss (ΔZ) and lesion depth were nearly linear (r ≧ 0.96) for both enamel and dentin. The slopes of the regression lines were between 0.99 and 1.02 except for ΔZ in dentine, which was 0.89. It was concluded that T-WIM is a suitable method for TMR on thick samples.
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Microradiography to evaluate bone growth into a rat mandibular defect.
Archives of oral biology, 2003Co-Authors: Jurjen Schortinghuis, Jan L. Ruben, Henny J. A. Meijer, Antonius L.j.j. Bronckers, Gerry M. Raghoebar, Boudewijn StegengaAbstract:Abstract Microradiography has been evaluated to measure bone healing into a 5.0 mm outer diameter mandibular defect in the rat. This method provides high-resolution radiographs of the defects that can be used for an accurate measurement of bone defect healing. In 12 rats, the defect widths of 42-day-old mandibular defects have been measured both using microradiographs and histological sections. The defect width±S.D. measured 3.42±0.98 mm microradiographically and 3.47±1.11 mm histologically. Both methods were accurate in determining defect widths but Microradiography has the advantage over histology that an image is obtained from the entire defect, making it possible to measure areas of bone growth.
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Influence of nail varnish on the remineralization of enamel single sections assessed by Microradiography and confocal laser scanning microscopy
Caries research, 1998Co-Authors: Y Iijima, Jan L. Ruben, O Takagi, Heinz Duschner, J. ArendsAbstract:Single–section techniques are attractive in enamel de– and remineralization investigations because they allow longitudinal studies in which mineral changes can be assessed by Microradiography (TMR). N
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Major Topics in Quantitative Microradiography of Enamel and Dentin: R Parameter, Mineral Distribution Visualization, and Hyper-Remineralization
Advances in dental research, 1997Co-Authors: J. Arends, Jan L. Ruben, D. InabaAbstract:Three aspects of quantitative transverse Microradiography are described and discussed: (1) the average mineral loss parameter of enamel or dentin lesions (R) in vol%; (2) mineral distribution visualization of lesions by means of a computer-assisted videodensitometric (CAV) method; and (3) the Microradiography of hyper-remineralized lesions. "R", defined as the average amount of mineral lost (or gained) in a lesion per unit lesion length, is a useful parameter in dental caries. The results show that, in vitro, R is about constant over the demineralization period for enamel and dentin. This was found even for quite different dissolution kinetics. R is strongly reduced by the presence of small amounts of fluoride in the demineralizing system. R of dentin lesions is smaller than for enamel lesions. We conclude that the mineral loss value (ΔZ) and the lesion depth (ld) are in general dependent parameters during in vitro or in situ studies on enamel or dentin. During lesion formation, mineral loss from the lesi...
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Shrinkage prevention of in vitro demineralized human dentine in transversal Microradiography.
Caries research, 1993Co-Authors: Jan L. Ruben, J. ArendsAbstract:The shrinkage of demineralized dentine is a serious problem in the assessment of mineral distributions and mineral changes by means of Microradiography. In this article it is shown that a treatment wi
Marie-charlotte D.n.j.m. Huysmans - One of the best experts on this subject based on the ideXlab platform.
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Transversal wavelength-independent Microradiography, a method for monitoring caries lesions over time, validated with transversal Microradiography.
Caries research, 2006Co-Authors: R.z. Thomas, Jan L. Ruben, J. De Vries, J.j. Ten Bosch, Marie-charlotte D.n.j.m. HuysmansAbstract:This paper describes a microradiographic method for measuring mineral concentration in a transversal geometry with thick (≤3.2 mm) sections: transversal wavelength-independent Microradiography (T-WIM). It was tested on bovine enamel and dentin samples in vitro, and the results were validated with those of transversal Microradiography (TMR). 48 enamel and 48 dentin samples (3.2 × 3.2 × 1.5 mm) were embedded in acrylic resin, randomly divided into six groups of 8 dentin or 8 enamel samples, and demineralized for 0 (sound control), 1, 2, 3, 4, or 5 weeks. For T-WIM, samples were imaged on film with polychromatic 40-kV Cu X-rays with an Al (0.25 mm)/Ni (0.02 mm) filter together with an aluminium/zinc step wedge. TMR slices (about 80 µm for enamel and about 130 µm for dentine) were subsequently cut from the centre of the samples and subjected to TMR. Microradiographs from both methods were digitized and image analysis software was used to calculate lesion depth and mineral loss. The relations between T-WIM and TMR results for mineral loss (ΔZ) and lesion depth were nearly linear (r ≧ 0.96) for both enamel and dentin. The slopes of the regression lines were between 0.99 and 1.02 except for ΔZ in dentine, which was 0.89. It was concluded that T-WIM is a suitable method for TMR on thick samples.
J. Arends - One of the best experts on this subject based on the ideXlab platform.
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Influence of nail varnish on the remineralization of enamel single sections assessed by Microradiography and confocal laser scanning microscopy
Caries research, 1998Co-Authors: Y Iijima, Jan L. Ruben, O Takagi, Heinz Duschner, J. ArendsAbstract:Single–section techniques are attractive in enamel de– and remineralization investigations because they allow longitudinal studies in which mineral changes can be assessed by Microradiography (TMR). N
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Major Topics in Quantitative Microradiography of Enamel and Dentin: R Parameter, Mineral Distribution Visualization, and Hyper-Remineralization
Advances in dental research, 1997Co-Authors: J. Arends, Jan L. Ruben, D. InabaAbstract:Three aspects of quantitative transverse Microradiography are described and discussed: (1) the average mineral loss parameter of enamel or dentin lesions (R) in vol%; (2) mineral distribution visualization of lesions by means of a computer-assisted videodensitometric (CAV) method; and (3) the Microradiography of hyper-remineralized lesions. "R", defined as the average amount of mineral lost (or gained) in a lesion per unit lesion length, is a useful parameter in dental caries. The results show that, in vitro, R is about constant over the demineralization period for enamel and dentin. This was found even for quite different dissolution kinetics. R is strongly reduced by the presence of small amounts of fluoride in the demineralizing system. R of dentin lesions is smaller than for enamel lesions. We conclude that the mineral loss value (ΔZ) and the lesion depth (ld) are in general dependent parameters during in vitro or in situ studies on enamel or dentin. During lesion formation, mineral loss from the lesi...
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Shrinkage prevention of in vitro demineralized human dentine in transversal Microradiography.
Caries research, 1993Co-Authors: Jan L. Ruben, J. ArendsAbstract:The shrinkage of demineralized dentine is a serious problem in the assessment of mineral distributions and mineral changes by means of Microradiography. In this article it is shown that a treatment wi
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Effect of fluoride mouthrinsing on caries lesion development in shark enamel: an in situ caries model study.
European Journal of Oral Sciences, 1991Co-Authors: Bjøorn Ögaard, Jan L. Ruben, Gunnar Rölla, Ton Dijkman, J. ArendsAbstract:Shark enamel consists of nearly pure fluorapatite and has been shown to demineralize in an in situ caries model. The present study was conducted to investigate whether additional fluoride supplementation in the form of mouthrinsing would inhibit lesion development in shark enamel. The study slabs of shark enamel were mounted in dental appliances. Six individuals wore the appliances while rinsing daily with a neutral 0.2% NaF solution for 4 wk. The specimens were analyzed by means of quantitative Microradiography, and the data compared with a previous study using untreated shark enamel and the same participants. It was found that fluoride rinsing did not measurably inhibit enamel demineralization in 4 wk. Scanning electron microradiographs showed that calcium fluoride-like material was not formed on shark enamel after neutral fluoride treatment, supporting a previous study. The present study indicates, therefore, that formation of a calcium fluoride-like material on the enamel surface may be essential for the cariostatic effect of topical agents.
R.z. Thomas - One of the best experts on this subject based on the ideXlab platform.
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Transversal wavelength-independent Microradiography, a method for monitoring caries lesions over time, validated with transversal Microradiography.
Caries research, 2006Co-Authors: R.z. Thomas, Jan L. Ruben, J. De Vries, J.j. Ten Bosch, Marie-charlotte D.n.j.m. HuysmansAbstract:This paper describes a microradiographic method for measuring mineral concentration in a transversal geometry with thick (≤3.2 mm) sections: transversal wavelength-independent Microradiography (T-WIM). It was tested on bovine enamel and dentin samples in vitro, and the results were validated with those of transversal Microradiography (TMR). 48 enamel and 48 dentin samples (3.2 × 3.2 × 1.5 mm) were embedded in acrylic resin, randomly divided into six groups of 8 dentin or 8 enamel samples, and demineralized for 0 (sound control), 1, 2, 3, 4, or 5 weeks. For T-WIM, samples were imaged on film with polychromatic 40-kV Cu X-rays with an Al (0.25 mm)/Ni (0.02 mm) filter together with an aluminium/zinc step wedge. TMR slices (about 80 µm for enamel and about 130 µm for dentine) were subsequently cut from the centre of the samples and subjected to TMR. Microradiographs from both methods were digitized and image analysis software was used to calculate lesion depth and mineral loss. The relations between T-WIM and TMR results for mineral loss (ΔZ) and lesion depth were nearly linear (r ≧ 0.96) for both enamel and dentin. The slopes of the regression lines were between 0.99 and 1.02 except for ΔZ in dentine, which was 0.89. It was concluded that T-WIM is a suitable method for TMR on thick samples.
J.j. Ten Bosch - One of the best experts on this subject based on the ideXlab platform.
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Transversal wavelength-independent Microradiography, a method for monitoring caries lesions over time, validated with transversal Microradiography.
Caries research, 2006Co-Authors: R.z. Thomas, Jan L. Ruben, J. De Vries, J.j. Ten Bosch, Marie-charlotte D.n.j.m. HuysmansAbstract:This paper describes a microradiographic method for measuring mineral concentration in a transversal geometry with thick (≤3.2 mm) sections: transversal wavelength-independent Microradiography (T-WIM). It was tested on bovine enamel and dentin samples in vitro, and the results were validated with those of transversal Microradiography (TMR). 48 enamel and 48 dentin samples (3.2 × 3.2 × 1.5 mm) were embedded in acrylic resin, randomly divided into six groups of 8 dentin or 8 enamel samples, and demineralized for 0 (sound control), 1, 2, 3, 4, or 5 weeks. For T-WIM, samples were imaged on film with polychromatic 40-kV Cu X-rays with an Al (0.25 mm)/Ni (0.02 mm) filter together with an aluminium/zinc step wedge. TMR slices (about 80 µm for enamel and about 130 µm for dentine) were subsequently cut from the centre of the samples and subjected to TMR. Microradiographs from both methods were digitized and image analysis software was used to calculate lesion depth and mineral loss. The relations between T-WIM and TMR results for mineral loss (ΔZ) and lesion depth were nearly linear (r ≧ 0.96) for both enamel and dentin. The slopes of the regression lines were between 0.99 and 1.02 except for ΔZ in dentine, which was 0.89. It was concluded that T-WIM is a suitable method for TMR on thick samples.