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Goro Takada - One of the best experts on this subject based on the ideXlab platform.
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Effect of N-Methyl-D-Aspartate and Potassium on Striatal Monoamine Metabolism in Immature Rat: An In Vivo Microdialysis Study
Neurochemical Research, 1998Co-Authors: Wako Nakajima, Akira Ishida, Maya Ogasawara, Goro TakadaAbstract:Effects of N-methyl-D-aspartate (NMDA) and potassium on 5-day-old rat's brain were examined. We measured extracellular striatal Monoamines such as dopamine (DA), 3,4 dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindole-3-acetic acid (5-HIAA) using intracerebral microdialysis. After 3 h stabilization, pups received varying concentrations of NMDA (1–3 mM) and potassium (200–800 mM) by intrastriatal perfusion for 32 minutes. Increasing the concentration of NMDA and potassium induced a dose related DA increase (p < 0.001), whereas DOPAC, HVA, and 5-HIAA decreased significantly. Five days later the same animals were sacrificed and the weight reduction of their cerebral hemispheres was measured. The weight of the drug perfused side was significantly reduced compared with that of the contralateral one. We examined next the relationship between the level of maximum DA and the relative hemisphere weight reduction. The DA peak was highly correlated with the hemisphere weight reduction (r = 0.70, n = 52, p < 0.001 in the NMDA group, r = 0.83, n = 30, p < 0.001 in the potassium group, respectively). These data show that each treatment alter striatal Monoamine Metabolism in immature rat brain and that the extracellular DA peak is a potential early indicator to estimate brain injury.
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EFFECT OF N-METHYL-D-ASPARTATE ON STRIATAL Monoamine Metabolism IN IMMATURE RAT BRAIN; IS DOPAMINE A QUANTITATIVE INDICATOR OF BRAIN INJURY?: AN IN VIVO MICRODIALYSIS STUDY † 981
Pediatric Research, 1997Co-Authors: Wako Nakajima, Akira Ishida, Hirokazu Arai, Goro TakadaAbstract:EFFECT OF N-METHYL-D-ASPARTATE ON STRIATAL Monoamine Metabolism IN IMMATURE RAT BRAIN; IS DOPAMINE A QUANTITATIVE INDICATOR OF BRAIN INJURY?: AN IN VIVO MICRODIALYSIS STUDY † 981
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Effect of anoxia on striatal Monoamine Metabolism in immature rat brain compared with that of hypoxia: an in vivo microdialysis study.
Brain research, 1996Co-Authors: Wako Nakajima, Akira Ishida, Goro TakadaAbstract:We examined in 5-day-old rats the effects of either anoxia or 8% hypoxia on extracellular Monoamines such as dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), norepinephrine (NE), 5-hydroxytryptamine (5-HT), and 5-hydroxyindole-3-acetic acid (5-HIAA) using in vivo microdialysis and subsequent HPLC. After stabilization 64 animals were exposed to 100% nitrogen for 16 min and 40 animals to 8% oxygen for 128 min. Both anoxia and hypoxia produced acute increase in the striatal extracellular DA (anoxia: P < 0.001, hypoxia: P < 0.01). Especially in anoxia, DA levels increased transiently to 2000-times the basal levels and 6-times higher than those in hypoxia. NE also increased in both anoxia and hypoxia. DOPAC and HVA decreased during hypoxia (P < 0.01 and P < 0.001, respectively), while those in anoxia were unchanged. In anoxia, decrease tendency of their levels were in short duration and that of 5-HIAA was followed by gradual increase (P < 0.001). These data demonstrated that brief exposure to anoxia or hypoxia had significant influence on striatal Monoamine Metabolism in immature brain and the pattern of change of Monoamine in anoxia was different from that in hypoxia.
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EFFECT OF ANOXIA ON STRIATAL Monoamine Metabolism IN IMMATURE RAT BRAIN COMPARED WITH THAT OF HYPOXIA : AN IN VIVO MICRODIALYSIS STUDY† 1378
Pediatric Research, 1996Co-Authors: Wako Nakajima, Akira Ishida, Hirokazu Arai, Yasushi Takahashi, Masamichi Tamura, Goro TakadaAbstract:EFFECT OF ANOXIA ON STRIATAL Monoamine Metabolism IN IMMATURE RAT BRAIN COMPARED WITH THAT OF HYPOXIA : AN IN VIVO MICRODIALYSIS STUDY† 1378
Mitsuhiro Furuse - One of the best experts on this subject based on the ideXlab platform.
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effects of chattonella antiqua on the swimming behavior and brain Monoamine Metabolism of juvenile yellowtail seriola quinqueradiata
Marine Pollution Bulletin, 2020Co-Authors: Yukihiko Matsuyama, Mitsuhiro Furuse, Yohei Shimasaki, Yuji OshimaAbstract:Abstract Being the precursor of serotonin and melatonin, dietary supplementation with tryptophan (TRP) may modulates behavior, stress responses, and antioxidant capacity in fish. In this study, effects of Chattonella exposure on the swimming behavior and brain Monoamine Metabolism of yellowtail fed a commercial diet (control diet) or that enriched by 1.5% L-TRP (TRP + diet) were investigated. A 7-day dietary TRP supplementation elevated spontaneous swimming speed of yellowtail and mitigated their behavioral response to Chattonella (250 cells/mL) exposure. A 30-day dietary TRP supplementation elevated growth of juvenile yellowtail. Lethal exposure to Chattonella (1000 cells/mL) significantly elevated the turnover rates of serotonin, dopamine, and norepinephrine Metabolism in fish fed control diet, but did not alter the serotonin turnover rate in fish fed TRP + diet. Our results suggested that dietary supplementation with TRP had potential to mitigate the stress response in yellowtail to Chattonella, partly via mediating their brain Monoamine Metabolism.
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dietary animal proteins alter Monoamine Metabolism in the brain
Animal Science Journal, 2012Co-Authors: Mao Nagasawa, Tatsuro Murakami, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu, Mitsuhiro FuruseAbstract:: Several amino acids have effects on mental function, including sedative, antidepressant-like and anxiolytic-like effects. However, the influence of integrated amino acid nutrition as protein constituents on mental function remains unclear. Therefore, the purpose of the present study was to compare the influence of chicken, pork and beef protein extracts on brain Monoamine Metabolism in mice. Changes in Monoamine levels and their turnover rates in the brain were induced by different protein sources. In particular, chicken protein group showed the highest norepinephrine levels in the hippocampus and hypothalamus, and beef protein extract caused an activation of the serotonergic system in the hypothalamus, although there were no significant differences in amino acid compositions of these protein extracts. Therefore, it was revealed that amino acid compositions in dietary protein did not induce alteration in Monoamine Metabolism. However, there were differences in small molecular peptides, such as creatine, carnosine and anserine levels in animal protein extracts. In conclusion, Monoamine Metabolism was altered by dietary protein sources. However, it was indicated that the alteration in Monoamine Metabolism may be independent from amino acid compositions in dietary protein. In addition, alteration in Monoamine Metabolism depending on the dietary protein sources may be induced by small molecular peptides.
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changes in brain Monoamine Metabolism of neonatal chicks under two different acute stress conditions
British Poultry Science, 2012Co-Authors: Kousuke Hamasu, Yusuke Kabuki, Shozo Tomonaga, D M Denbow, Mitsuhiro FuruseAbstract:1. The purpose of the present study was to clarify brain Monoamine Metabolism during two different conditions of acute stress by quantifying changes in the brain of neonatal chicks exposed to either restraint with isolation, or fasting stress. 2. Under restraint with isolation-induced stress, dopaminergic Metabolism was clearly stimulated. 3. During fasting stress, dopaminergic activity, serotonergic and norepinephrinergic Metabolisms were stimulated. 4. It was concluded that brain Monoamine Metabolism of chicks is differentially affected by stressors.
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intracerebroventricular injection of orexin a stimulates Monoamine Metabolism but not hpa axis in neonatal chicks
Neuroscience Letters, 2010Co-Authors: Sachiko Katayama, Kazutaka Shigemi, Mark A Cline, Mitsuhiro FuruseAbstract:We investigated the effects of intracerebroventricular (ICV) injection of orexin-A on plasma corticosterone (CORT) concentration and brain Monoamine Metabolism to clarify the mechanism by which ICV orexin-A induced arousal in chicks. In Experiment 1, plasma CORT concentrations were measured as an indicator of hypothalamic-pituitary-adrenal (HPA) axis activity. There was no significant difference in CORT concentration between the control and orexin-A administered groups. In Experiment 2, the concentrations of Monoamines (norepinephrine, dopamine and serotonin), their metabolites, and their metabolic turnover rates in the telencephalon, mesencephalon, and diencephalon were investigated. All metabolic turnover rates studied were increased at all brain sites after ICV orexin-A injection. In conclusion, the HPA axis does not appear to be involved in arousal-inducing mechanisms of orexin-A in neonatal chicks; however, several Monoaminergic systems do.
N P Sharova - One of the best experts on this subject based on the ideXlab platform.
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detection of active proteasome structures in brain extracts proteasome features of august rat brain with violations in Monoamine Metabolism
Oncotarget, 2017Co-Authors: Pavel A Erokhov, Yulia V Lyupina, Alexandra S Radchenko, A A Kolacheva, Y O Nikishina, N P SharovaAbstract:// Pavel A. Erokhov 1, * , Yulia V. Lyupina 1, * , Alexandra S. Radchenko 1 , Anna A. Kolacheva 2 , Yulia O. Nikishina 2 and Natalia P. Sharova 1 1 Laboratory of Biochemistry of Ontogenesis Processes, N.K. Koltsov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia 2 Laboratory of Neural and Neuroendocrine Regulations, N.K. Koltsov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia * These authors have contributed equally to this work Correspondence to: Natalia P. Sharova, email: npsharova@bk.ru Keywords: proteasome structure, immune proteasomes, rat brain, violations in Monoamine Metabolism, native electrophoresis Received: May 26, 2017 Accepted: July 23, 2017 Published: August 10, 2017 ABSTRACT The aim of this work was to detect changes in proteasome pools of brain parts of August rats with Monoamine Metabolism violations in comparison with that of control Wistar rats. To reveal active proteasome structures, a method of native electrophoresis for the analysis of crude tissue fractions was developed. By means of this method and following Western blotting, the most pronounced changes in reorganization of proteasome structures were detected in proteasome pool of the brain cortex of August rats. Main findings are the enhanced expression of immune proteasome subtypes containing proteolytic subunit LMP2 and activator PA28αβ as well as immune proteasome subtypes containing proteolytic subunit LMP7 and activator PA700 and simultaneously decreased expression of subtypes with subunit LMP2 and activator PA700 in the brain cortex of August rats compared to that of Wistar rats. These results were indirectly confirmed by SDS PAGE method followed by Western blotting, which showed the increased quantities of immune subunits and proteasome activators in the brain cortex of August rats compared to that of Wistar rats. Immune proteasomes were revealed by immunohistochemistry in neurons, but not in glial cells of August and Wistar rat cortex. The detected reorganization of proteasome pools is likely to be important for production of special peptides to provide the steady interaction between neurons and adaptation of central nervous system to conditions caused by Monoamine Metabolism deviations.
Thomas Hedner - One of the best experts on this subject based on the ideXlab platform.
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Cerebral and Adrenal Monoamine Metabolism in the Growth-Retarded Rat Fetus under Normoxia and Hypoxia
Pediatric Research, 1992Co-Authors: Magnus Thordstein, Thomas HednerAbstract:The effect of intrauterine growth retardation (IUGR) on cerebral and adrenal Monoamine Metabolism was studied in the fetuses of eight nulliparous rat dams after unilateral uterine artery ligation on d 18 of gestation. On d 22 (term = 23 d), four dams were subjected to normoxia and four to hypoxia (10% O2) for 58 min while their hemodynamics and blood gases were monitored. An inhibitor of L-aromatic-decarboxylase (3-hydroxybensylhydrazine) was infused to measure Monoamine synthesis rate. After decapitation of the dam, fetuses were delivered by sectio, decapitated, and dissected at −5±C. The body, liver, forebrain, brainstem, and adrenal glands were weighed, and concentrations of Monoamine precursors, transmitters, and metabolites were assessed in the three latter organs. The weights of liver and forebrain were reduced in fetuses with IUGR, whereas brainstem and adrenal weights were unaltered. Epinephrine content in adrenals was reduced in proportion to body weight under normoxia but failed to increase under hypoxia as it did in appropriately grown fetuses. There were only minor changes in Monoamine Metabolism in the brainstem. In the forebrain, however, marked changes were seen, mainly in serotonin Metabolism: under normoxia, fetuses with IUGR had decreased levels of serotonin and its metabolite 5-hydroxyindole acetic acid. Under hypoxia, appropriately grown fetuses reduced their concentrations of these substances, whereas fetuses with IUGR paradoxically increased their synthetic activity. It is concluded that a disturbance of central nervous serotonin Metabolism prevails in growth-retarded rat fetuses in late gestation and that this disturbance depends on the degree of growth retardation and the degree of perinatal stress.
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cerebral and adrenal Monoamine Metabolism in the growth retarded rat fetus under normoxia and hypoxia
Pediatric Research, 1992Co-Authors: Magnus Thordstein, Thomas HednerAbstract:: The effect of intrauterine growth retardation (IUGR) on cerebral and adrenal Monoamine Metabolism was studied in the fetuses of eight nulliparous rat dams after unilateral uterine artery ligation on d 18 of gestation. On d 22 (term = 23 d), four dams were subjected to normoxia and four to hypoxia (10% O2) for 58 min while their hemodynamics and blood gases were monitored. An inhibitor of L-aromatic-decarboxylase (3-hydroxybensylhydrazine) was infused to measure Monoamine synthesis rate. After decapitation of the dam, fetuses were delivered by sectio, decapitated, and dissected at -5 degrees C. The body, liver, forebrain, brainstem, and adrenal glands were weighed, and concentrations of Monoamine precursors, transmitters, and metabolites were assessed in the three latter organs. The weights of liver and forebrain were reduced in fetuses with IUGR, whereas brainstem and adrenal weights were unaltered. Epinephrine content in adrenals was reduced in proportion to body weight under normoxia but failed to increase under hypoxia as it did in appropriately grown fetuses. There were only minor changes in Monoamine Metabolism in the brainstem. In the forebrain, however, marked changes were seen, mainly in serotonin Metabolism: under normoxia, fetuses with IUGR had decreased levels of serotonin and its metabolite 5-hydroxyindole acetic acid. Under hypoxia, appropriately grown fetuses reduced their concentrations of these substances, whereas fetuses with IUGR paradoxically increased their synthetic activity. It is concluded that a disturbance of central nervous serotonin Metabolism prevails in growth-retarded rat fetuses in late gestation and that this disturbance depends on the degree of growth retardation and the degree of perinatal stress.
Byron C Jones - One of the best experts on this subject based on the ideXlab platform.
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abnormal rat brain Monoamine Metabolism in iron deficiency anemia
Journal of Nutritional Biochemistry, 1995Co-Authors: Qing Chen, John L Beard, Byron C JonesAbstract:Iron deficiency in rats produces numerous alterations in brain Metabolism as assessed by in vitro techniques. We used a new method of in vivo microdialysis to study the effect of acute iron deficiency anemia on rat brain Monoamine Metabolism. This method was used to sample extracellular fluid from an implanted microdialysis probe in the caudate putamen from freely moving animals. Method validation experiments showed that steady-state levels of dopamine, norepinephrine, and their metabolites were obtained only after 5 to 7 days of surgical recovery and with prior perfusion of the brain region. Caudate putamen dopamine was significantly increased 30% and 40% in fasted light-exposed and 2-hr-fed dark-exposed iron deficient anemic rats (hemoglobin in vitro demonstrations of down-regulation of dopamine D 2 receptors, they also suggest that uptake and processing of Monoamines is significantly perturbed by iron deficiency.