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B. J. Tindall - One of the best experts on this subject based on the ideXlab platform.
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The subgenus names Moraxella and Branhamella (in the genus Moraxella) are not in accordance with the International Code of Nomenclature of Bacteria and are therefore not validly published: Supplementary information to Opinion 83. Judicial Commission
International journal of systematic and evolutionary microbiology, 2014Co-Authors: B. J. TindallAbstract:The publication of Opinion 83, which dealt with the valid publication of the subgenus names Moraxella and Branhamella (in the genus Moraxella), has highlighted a problem relating to the absence of descriptions associated with these names at the time they were effectively published. This calls into question whether the ruling outlined in Opinion 83, that these names should have qualified for inclusion on the Approved Lists of Bacterial Names, and their inclusion on Validation List 15 are not in accordance with Rule 27 of the International Code of Nomenclature of Bacteria governing the valid publication of a name. The subgenus names Moraxella and Branhamella (in the genus Moraxella) are not to be considered to be included on the Approved Lists of Bacterial Names, nor are they to be considered to be validly published by inclusion on Validation List 15.
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The subgenus names Moraxella subgen. Moraxella and Moraxella subgen. Branhamella and the species names included within these taxa should have been included on the Approved Lists of Bacterial Names and a ruling on the proposal to make changes to Rule
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: B. J. TindallAbstract:The Judicial Commission of the International Committee for Systematics of Prokaryotes rules that the following names should have been included on the Approved Lists of Bacterial Names, Moraxella (subgen. Branhamella Bovre 1979), Moraxella (subgen. Moraxella Lwoff 1939), Moraxella (subgen. Branhamella Bovre 1979) catarrhalis, Moraxella (subgen. Branhamella Bovre 1979) caviae, Moraxella (subgen. Branhamella Bovre 1979) ovis, Moraxella (subgen. Moraxella Lwoff 1939) atlantae, Moraxella (subgen. Moraxella Lwoff 1939) bovis, Moraxella (subgen. Moraxella Lwoff 1939) lacunata, Moraxella (subgen. Moraxella Lwoff 1939) nonliquefaciens, Moraxella (subgen. Moraxella Lwoff 1939) osloensis, Moraxella (subgen. Moraxella Lwoff 1939) phenylpyruvica. Proposals to alter Rule 34a were rejected.
José F. Fernández-garayzábal - One of the best experts on this subject based on the ideXlab platform.
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Moraxella porci sp. nov., isolated from pigs
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Ana I. Vela, Cristina Sánchez-porro, Virginia Aragon, A. Olvera, Lucas Domínguez, Antonio Ventosa, José F. Fernández-garayzábalAbstract:Nine Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria were isolated from pigs affected by different pathological processes. Phenotypic and genotypic methods were adopted to determine the relationships of these new isolates to recognized species of the genus Moraxella. Analysis of the 16S rRNA gene sequences demonstrated that the clinical isolates represented a new lineage within the genus Moraxella. The isolates were closely related to Moraxella cuniculi and Moraxella pluranimalium with 16S rRNA gene sequence similarities of 98.1 % and 99.1 %, respectively. The isolates displayed DNA–DNA relative binding ratios of 74 % to each other, but distinctly lower levels of DNA–DNA hybridization were observed with phylogenetically closely related Moraxellae (
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Moraxella pluranimalium sp. nov., isolated from animal specimens.
International journal of systematic and evolutionary microbiology, 2009Co-Authors: Ana I. Vela, Cristina Sánchez-porro, Virginia Aragon, Lucas Domínguez, Antonio Ventosa, E Arroyo, M V Latre, M Cerdà-cuéllar, José F. Fernández-garayzábalAbstract:Four unusual Gram-negative, catalase-positive, oxidase-positive, coccus-shaped bacteria isolated from one sheep and three pigs were characterized using phenotypic and molecular genetic methods. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Moraxella, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that the isolates represent a novel subline within the genus Moraxella. The most closely related species in phylogenetic terms was Moraxella cuniculi, with 16S rRNA gene sequence similarity of 97.9 % to the type strain CCUG 2154(T), although the DNA-DNA relatedness value was only 29 %. The novel isolates were readily distinguished from all recognized Moraxella species by means of physiological and biochemical tests. On the basis of molecular genetic and phenotypic evidence, therefore, the four isolates represent a novel species of the genus Moraxella, for which the name Moraxella pluranimalium sp. nov. is proposed. The type strain is 248-01(T) (=CECT 7295(T) =CCUG 54913(T)).
John A. Angelos - One of the best experts on this subject based on the ideXlab platform.
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Differentiation of Moraxella bovoculi sp. nov. from other coccoid Moraxellae by the use of polymerase chain reaction and restriction endonuclease analysis of amplified DNA.
Journal of Veterinary Diagnostic Investigation, 2007Co-Authors: John A. Angelos, Louise M. BallAbstract:Moraxella ovis was historically the only coccoid Moraxella identified in cultures of ocular fluid from cattle with infectious bovine keratoconjunctivitis (IBK) and could be morphologically and biochemically differentiated from Moraxella bovis. Moraxella bovoculi sp. nov. is a recently characterized Moraxella isolated from ulcerated eyes of calves with IBK in northern California in 2002. Like Moraxella ovis, M. bovoculi sp. nov. is a gram-negative coccus/diplococcus. All 18 original isolates of M. bovoculi sp. nov. possessed phenylalanine deaminase (PADase) activity and could therefore be differentiated from M. ovis and M. bovis. During the characterization of 44 additional isolates of hemolytic gram-negative cocci that were cultured from ulcerated eyes of IBK-affected calves, 2 PADase-negative isolates were identified that could not be differentiated biochemically from M. ovis; however, the DNA sequence of the 16S-23S intergenic spacer region (ISR) of the isolates matched the 16S-23S ISR DNA sequence of M. bovoculi sp. nov. To facilitate the identification of PADase-negative Moraxellae, a polymerase chain reaction (PCR) coupled with restriction enzyme digestion analysis of amplified DNA was developed. Amplification of the 16S-23S ISR followed by AfaI digestion of amplified DNA could differentiate M. bovoculi sp. nov. from M. ovis and other Moraxellae. The DNA sequence analysis of the amplified 16S-23S ISR from the 42 PADase-positive isolates of hemolytic gram-negative cocci indicated that all were M. bovoculi sp. nov. and all possessed an AfaI site. A PCR coupled with restriction analysis of amplified DNA can aid in identifying M. bovoculi sp. nov.
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Identification and characterization of complete RTX operons in Moraxella bovoculi and Moraxella ovis.
Veterinary microbiology, 2007Co-Authors: John A. Angelos, Louise M. Ball, John F HessAbstract:To determine if Moraxella bovoculi (M. bovoculi), a recently characterized coccoid Moraxella that was isolated from the eyes of calves affected with infectious bovine keratoconjunctivitis (IBK), and Moraxella ovis (M. ovis), originally isolated from sheep with conjunctivitis, possessed genes encoding RTX proteins, genomic DNA was amplified with oligonucleotide primers targeting RTX operon genes of Moraxella bovis (M. bovis). Complete classical RTX operons composed of RTXCABD genes closely linked to a putative secretion accessory protein encoding gene (tolC) were identified in M. bovoculi and M. ovis and were designated mbvCABDtolC and movCABDtolC, respectively. These genes were closely related to M. bovis mbxCABDtolC. Polyclonal rabbit antiserum against the carboxy terminus of M. bovoculi MbvA neutralized hemolytic activity of both M. bovoculi and M. ovis; this antiserum did not neutralize the hemolytic activity of M. bovis. M. bovoculi and M. ovis possess genes that encode proteins related to pathogenic factors of M. bovis.
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Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: John A. Angelos, Louise M. Ball, Phillip Q Spinks, L. W. GeorgeAbstract:Eighteen isolates of a Gram-negative coccus (strain 237(T)) were cultured from the eyes of dairy and beef calves affected with infectious bovine keratoconjunctivitis (IBK; 'pinkeye') in northern California, USA, during summer 2002. These isolates had near full-length (1397 bp) 16S rRNA gene sequences that clustered into three groups with 99.9 % sequence similarity. On the basis of 16S rRNA gene sequence, the isolates were most closely associated with Moraxella bovis and Moraxella ovis in clade I of the classical Moraxellae. Biochemically, the novel isolates could be distinguished from the other members of the genus Moraxella isolated from animals on the basis of phenylalanine deaminase activity. The results of partial sequence analysis of six housekeeping genes, the 16S-23S rRNA gene interspacer region and partial 23S rRNA gene provide strong support for the inclusion of these isolates in a novel taxon, for which the name Moraxella bovoculi sp. nov. is proposed. The type strain is strain 237(T) (=ATCC BAA-1259(T)=CCUG 52049(T)).
Y. Richard - One of the best experts on this subject based on the ideXlab platform.
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Moraxella boevrei sp. nov., a new Moraxella species found in goats.
International journal of systematic bacteriology, 1997Co-Authors: A. Kodjo, Y. Richard, Tone TonjumAbstract:Six Moraxella-like strains that formed a phenotypically homogeneous group were isolated from the nasal flora of healthy goats. Total genomic DNA-DNA hybridization, rRNA gene restriction pattern, DNA base composition, and genetic transformation studies were performed to determine the relationships of these bacteria to species belonging to the genus Moraxella and other fastidious gram-negative species. The new group of isolates was very homogeneous, as shown by rRNA gene restriction fragment length patterns (ribotyping), and these organisms displayed high relative binding ratios (RBRs) to each other in DNA-DNA hybridization experiments (RBRs, ≥ 58%) but distinctly lower levels of DNA homology with all other species investigated. However, the RBRs obtained with species of the genus Moraxella were higher than the RBRs obtained with all other gram-negative strains examined. Although the new strains had most of the Moraxella bovis phenotypic characteristics except nitrate reduction, quantitative and qualitative genetic transformation data led to the conclusion that they belong to a distinct new cluster in the genus Moraxella. The results of this study, combined with the general morphological and phenotypic profiles of the new strains, are consistent with the creation of a new Moraxella species, for which the name Moraxella boevrei is proposed. Strain 88365 (= ATCC 700022 = CCUG 35435 = NCTC 12925 = CIP 104716) is the type strain of M. boevrei.
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Moraxella caprae sp nov a new member of the classical Moraxellae with very close affinity to Moraxella bovis
International Journal of Systematic and Evolutionary Microbiology, 1995Co-Authors: A. Kodjo, Tone Tonjum, Y. Richard, Kjell BovreAbstract:Eight phenotypically homogenous Moraxella-like strains were isolated from the nasal flora of healthy goats. Total genomic DNA-DNA hybridization, DNA base composition determination, and genetic transformation studies were performed to determine the relationships of these bacteria to the classical Moraxellae. The eight new isolates exhibited very high levels of genetic affinity to Moraxella bovis, as shown by quantitative and qualitative genetic transformation data, and exhibited high DNA-DNA relative binding ratios to each other (63% or more) but lower levels of DNA homology with all of the other species investigated, including the closely related classical Moraxellae. Our results, combined with the general morphologic and phenotypic profiles of these organisms, indicate that they should be classified with the classical Moraxellae, and we propose the name Moraxella caprae for them. Strain 8897 (= CCUG 33297 = NCTC 12877) is the type strain of M. caprae.
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Isolation and characterization of a new biovar of Moraxella bovis from healthy caprine nasal swabs
Small Ruminant Research, 1994Co-Authors: Angeli Kodjo, A. Dorier, C. Lerondelle, Y. RichardAbstract:Twenty-four strains of presumptive Moraxella and Branhamella spp. isolated from healthy small ruminant nasal swabs and nine collection strains of the two above genera were compared by means of physiological, enzyme hydrolysing procedures and experimental infectivity tests. Four physiological groups were established. One new biovar of Moraxella, intermediate between Moraxella bovis and Moraxella lacunata was identified from goat nasal isolates. Corneal swabbing and instillation in mice of such Moraxella strain gave a mild transitory photophobia as did the collection strain CIP 7039 Moraxella bovis used in this study. Taxonomic studies are warranted to determine whether it is a new Moraxella species or a Moraxella bovis subspecies.
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identification of Moraxella bovis and related species from calves with ibk and goats by qualitative genetic transformation assay
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 1994Co-Authors: Angeli Kodjo, P Exbrayat, Y. RichardAbstract:ummary Eight Moraxella bovis strains isolated from bovine pink eye, 16 Moraxella bovis related strains isolated from healthy goats nasal flora, one nonhemolytic Moraxella sp. isolated from bovine conjunctivitis and different collection strains of Moraxella and Branhamella genus were studied through the combined use of qualitative genetic transformation assay and the ability to grow on a Moraxella bovis defined medium (medium MB). Crude DNA extracted from the strains studied were used to transform two mutant auxotroph competent strains of Moraxella bovis CIP 103741 and CIP 103743. Non-specific positive transformation was obtained with mutant assay strain CIP 103743 when treated with DNA from caprine or bovine Moraxella strains, collection strains of Moraxella bovis, Moraxella lacunata, Moraxella nonliquefaciens and strains of Branhamella genus, whereas specific transformation was observed with mutant assay CIP 103741 when treated only with DNA from all the Moraxella bovis (collection and clinical isolates) and 14 of the 16 caprine Moraxella bovis related strains. The specificity and the simplicity of the test make it suitable for use in clinical laboratories. Resume Huit souches de Moraxella bovis isolees de conjonctivite infectieuse bovine, seize souches apparentees a Moraxella bovis d'origine caprine, une souche de Moraxella sp. nonhemolytic et diverses souches de collection de Moraxella et de Branhamella ont ete etudiees par l'utilisation combinee de test qualitatif de transformation genetique et de croissance sur milieu defini (milieu MB). L'ADN des souches etudiees a servi a transformer deux souches mutantes auxotrophes de Moraxella bovis (CIP 103741 et CIP 103743). Des transformations non specifiques ont ete obtenues avec la souche mutante CIP 103743 traitee par l'ADN de Moraxella bovis, Moraxella lacunata, Moraxella nonliquefaciens, Branhamella sp. et l'ADN des souches caprines et bovines. A l'inverse, seul l'ADN des souches de Moraxella bovis a transforme significativement la souche mutante CIP 103741. Un resultat positif a ete obtenu avec les huit souches bovines de Moraxella bovis isolees de conjonctivite bovine et quatorze des seize souches d'origine caprine. La specificite et la simplicite de ce test constituent des atouts pour son utilisation dans le diagnostic de laboratoire de Moraxella bovis.
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Identification of Moraxella bovis and related species from calves with IBK and goats by qualitative genetic transformation assay.
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B, 1994Co-Authors: A. Kodjo, P Exbrayat, Y. RichardAbstract:Eight Moraxella bovis strains isolated from bovine pink eye, 16 Moraxella bovis related strains isolated from healthy goats nasal flora, one nonhemolytic Moraxella sp. isolated from bovine conjunctivitis and different collection strains of Moraxella and Branhamella genus were studied through the combined use of qualitative genetic transformation assay and the ability to grow on a Moraxella bovis defined medium (medium MB). Crude DNA extracted from the strains studied were used to transform two mutant auxotroph competent strains of Moraxella bovis CIP 103741 and CIP 103743. Non-specific positive transformation was obtained with mutant assay strain CIP 103743 when treated with DNA from caprine or bovine Moraxella strains, collection strains of Moraxella bovis, Moraxella lacunata, Moraxella nonliquefaciens and strains of Branhamella genus, whereas specific transformation was observed with mutant assay CIP 103741 when treated only with DNA from all the Moraxella bovis (collection and clinical isolates) and 14 of the 16 caprine Moraxella bovis related strains. The specificity and the simplicity of the test make it suitable for use in clinical laboratories.
Monique Gillis - One of the best experts on this subject based on the ideXlab platform.
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Taxonomy of Moraxellaceae fam. nov., a New Bacterial Family To Accommodate the Genera Moraxella, Acinetobacter, and Psychrobacter and Related Organisms
2013Co-Authors: Rudi Rossau, Monique Gillis, A. Van Landschoot, J. De LeyAbstract:DNA-rRNA hybridization results showed that members of the genus Moraxella, members of the genus Psychrobacter and their relatives, members of the genus Acinetobacter, the false neisseriae, and two misnamed Alysiella strains constitute a separate genotypic cluster. A new family, Moraxellaceae, is proposed to accommodate these organisms. The genus Moraxella is the type genus. Within the Moraxellaceae two main groups can be distinguished. One group includes the Acinetobacter species. The other group can be subdivided in four subgroups consisting of (i) the authentic Moraxellae (Moraxella lacunata, Moraxella yonliquefaciens, Moraxella bovis, Moraxella catarrhalis, Moraxella caviae, Moraxella ovis, Moraxella cuniculi, and two misnamed Alysiella strains), (ii) the generically misnamed taxon Moraxella osloensis, (iii) the generically misnamed taxon Moraxella atlantae, and (iv) the generically misnamed taxon Moraxella phenylpyruvica, Psychrobacter immobilis, and allied organisms. The Moraxellaceae cluster belongs to the class Proteobacteria and is a member of superfamily 11, which includes the authentic pseudomonads and related organisms. It is not related to the family Neisseriaceae. The genus Moraxella, which was first proposed by Lwoff in 1939, was placed in the family Neisseriaceae in 1968 by Henriksen and Bqjvre on the basis of phenotypic similaritie
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Moraxella lincolnii sp. nov., isolated from the human respiratory tract, and reevaluation of the taxonomic position of Moraxella osloensis.
International journal of systematic bacteriology, 1993Co-Authors: Peter Vandamme, Monique Gillis, Marc Vancanneyt, Bart Hoste, Karel Kersters, Enevold FalsenAbstract:A polyphasic taxonomic study was performed to determine the relationships of 10 Moraxella-like strains isolated mainly from the human respiratory tract in Sweden. Two of the strains formed a separate subgroup on the basis of both their protein contents and their fatty acid contents. However, the overall protein and fatty acid profiles revealed that all 10 strains were highly related. Representative strains of the two subgroups exhibited high DNA binding values (98%) with each other and had an identical DNA base ratio (44 mol% G+C). DNA-rRNA hybridizations revealed that this taxon can be included in the genus Moraxella, which is only distantly related to phenotypically similar genera, such as the genera Neisseria and Kingella. The results of an extensive phenotypic analysis indicated that the general biochemical profile of the 10 strains conforms with the description of the genus Moraxella given in Bergey's Manual of Systematic Bacteriology. We therefore consider these organisms members of a new Moraxella species, for which the name Moraxella lincolnii is proposed. Furthermore, we also conclude that Moraxella osloensis belongs, genotypically as well as phenotypically, to the genus Moraxella.
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Polyphasic taxonomy leading to the proposal of Moraxella canis sp. nov. for Moraxella catarrhalis-like strains.
International journal of systematic bacteriology, 1993Co-Authors: Geert Jannes, Monique Gillis, Peter Vandamme, Mario Vaneechoutte, M. Lannoo, M. Vancanneyt, Gerda Verschraegen, H. Van Heuverswyn, R. RossauAbstract:The taxonomic position of a group of 16 Moraxella catarrhalis-like strains, isolated mainly from dogs, was examined by using morphological tests, biochemical tests, serology, ribotyping with oligonucleotide probes, polymerase chain reaction typing of the 16S rRNA gene and the 16S-23S rRNA gene spacer region, polyacrylamide gel electrophoresis of total proteins, fatty acid profiles, moles percent G+C, dot spot and in-solution DNA-DNA hybridizations, and DNA-rRNA hybridizations. It was found that these organisms constitute a distinct cluster within the genus Moraxella. Since they differ genotypically as well as phenotypically from previously described Moraxella species, a new species, Moraxella canis, is proposed to accommodate these isolates. The type strain is LMG 11194 (= N7 = CCUG 8415A).
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taxonomy of Moraxellaceae fam nov a new bacterial family to accommodate the genera Moraxella acinetobacter and psychrobacter and related organisms
International Journal of Systematic and Evolutionary Microbiology, 1991Co-Authors: Rudi Rossau, A Van Landschoot, Monique GillisAbstract:DNA-rRNA hybridization results showed that members of the genus Moraxella, members of the genus Psychrobacter and their relatives, members of the genus Acinetobacter, the false neisseriae, and two misnamed Alysiella strains constitute a separate genotypic cluster. A new family, Moraxellaceae, is proposed to accommodate these organisms. The genus Moraxella is the type genus. Within the Moraxellaceae two main groups can be distinguished. One group includes the Acinetobacter species. The other group can be subdivided in four subgroups consisting of (i) the authentic Moraxellae (Moraxella lacunata, Moraxella nonliquefaciens, Moraxella bovis, Moraxella catarrhalis, Moraxella caviae, Moraxella ovis, Moraxella cuniculi, and two misnamed Alysiella strains), (ii) the generically misnamed taxon Moraxella osloensis, (iii) the generically misnamed taxon Moraxella atlantae, and (iv) the generically misnamed taxon Moraxella phenylpyruvica, Psychrobacter immobilis, and allied organisms. The Moraxellaceae cluster belongs to the class Proteobacteria and is a member of superfamily II, which includes the authentic pseudomonads and related organisms. It is not related to the family Neisseriaceae.
