The Experts below are selected from a list of 49416 Experts worldwide ranked by ideXlab platform
Xiaopeng Zhou - One of the best experts on this subject based on the ideXlab platform.
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maize dwarf Mosaic disease in different regions of china is caused by sugarcane Mosaic Virus
Archives of Virology, 2002Co-Authors: J X Jiang, Xiaopeng ZhouAbstract:Sugarcane Mosaic Virus (SCMV) was detected in all 62 maize samples collected from eight maize-growing provinces in China showing dwarf Mosaic symptoms by immunocapture reverse-transcription polymerase chain reaction (RT-PCR). Maize dwarf Mosaic Virus (MDMV), Sorghum Mosaic Virus (SrMV) and Johnsongrass Mosaic Virus (JGMV), however, were not detected in any of the samples by RT-PCR. Eleven cDNA fragments of approximately 0.8 kilobases covering most of the coat protein (CP) gene of SCMV were sequenced and sequence analysis indicates that these eleven isolates share 98.1 to 100 % identity at the amino acid level. Sequence comparison and phylogenetic analysis of the CP genes from the eleven Chinese isolates as well as 21 SCMV subgroup Virus isolates indicate that the eleven Chinese Virus isolates were closely related to SCMV with 97.0 to 98.1 % sequence identity at the amino acid level, while relatively lower sequence identity was found with MDWV, SrMV or JGMV. The results indicate that the Chinese isolates are members of the SCMV species, and thus, SCMV can be considered as the most common and important potyVirus infecting maize in China.
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maize dwarf Mosaic disease in different regions of china is caused by sugarcane Mosaic Virus
Archives of Virology, 2002Co-Authors: J X Jiang, Xiaopeng ZhouAbstract:Sugarcane Mosaic Virus (SCMV) was detected in all 62 maize samples collected from eight maize-growing provinces in China showing dwarf Mosaic symptoms by immunocapture reverse-transcription polymerase chain reaction (RT-PCR). Maize dwarf Mosaic Virus (MDMV), Sorghum Mosaic Virus (SrMV) and Johnsongrass Mosaic Virus (JGMV), however, were not detected in any of the samples by RT-PCR. Eleven cDNA fragments of approximately 0.8 kilobases covering most of the coat protein (CP) gene of SCMV were sequenced and sequence analysis indicates that these eleven isolates share 98.1 to 100 % identity at the amino acid level. Sequence comparison and phylogenetic analysis of the CP genes from the eleven Chinese isolates as well as 21 SCMV subgroup Virus isolates indicate that the eleven Chinese Virus isolates were closely related to SCMV with 97.0 to 98.1 % sequence identity at the amino acid level, while relatively lower sequence identity was found with MDWV, SrMV or JGMV. The results indicate that the Chinese isolates are members of the SCMV species, and thus, SCMV can be considered as the most common and important potyVirus infecting maize in China.
H D Mignouna - One of the best experts on this subject based on the ideXlab platform.
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variants of east african cassava Mosaic Virus and its distribution in double infections with african cassava Mosaic Virus in nigeria
Plant Disease, 2003Co-Authors: Francis O. Ogbe, Alfred G. O. Dixon, G Thottappilly, G I Atiri, H D MignounaAbstract:In a survey for cassava Mosaic begomoViruses conducted in 1997 and 1998 in Nigeria, East African cassava Mosaic Virus (EACMV) was detected by the polymerase chain reaction together with African cassava Mosaic Virus (ACMV) in 27 out of 290 cassava leaf samples of infected plants from 254 farmers' fields in five agroecological zones. One plant was infected with EACMV only. Five variant isolates of EACMV were observed based on their reactions to primers that could detect Cameroonian and East African strains of EACMV. Isolates of variants 1 and 3 occurred mostly in the derived or coastal and southern Guinea savannahs, while variants 4 and 5 predominated in the humid forest region. Isolates of variant 2 were widely distributed across the three agroecologies. EACMV was not detected in the northern Guinea savannah and arid and semiarid zones. Most doubly infected plants showed more severe symptoms than plants with single infection. Occurrence of EACMV variants together with ACMV detection and information about their distribution in Nigeria could be used for the selection of cassava clones in cassava Mosaic disease resistance programs.
Claude M Fauquet - One of the best experts on this subject based on the ideXlab platform.
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first report of the presence of east african cassava Mosaic Virus in cameroon
Plant Disease, 1998Co-Authors: V N Fondong, J S Pita, C Rey, R N Beachy, Claude M FauquetAbstract:Cassava Mosaic disease (CMD) occurs in all cassava-growing regions of Africa, India, and Sri Lanka. Characterized by Mosaic and distortion of cassava leaves and reduced plant growth, causing high yield losses, CMD is caused by geminiViruses (genus BegomoVirus, family Geminiviridae) transmitted through infected cuttings or by the whitefly, Bemisia tabaci. Three such geminiViruses have been described: African cassava Mosaic Virus (ACMV) occurs in most of the cassava-producing zones of Africa; East African cassava Mosaic Virus (EACMV) in East Africa; and Indian cassava Mosaic Virus (ICMV) in the Indian subcontinent (1). The two components of ACMV and ICMV genomes, DNA-A and DNA-B, have been sequenced; only DNA-A of EACMV has been identified and sequenced. Variations in symptom expression and severity within the same cassava variety have been observed in Cameroon. To determine the nature of the Virus species inducing such variations, 50 samples were collected from CMD-infected plants in the savannah and rainf...
A. R. R. Cruz - One of the best experts on this subject based on the ideXlab platform.
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rnai based enhanced resistance to cowpea severe Mosaic Virus and cowpea aphid borne Mosaic Virus in transgenic cowpea
Plant Pathology, 2014Co-Authors: A. R. R. Cruz, Francisco J. L. AragãoAbstract:Cowpea (Vigna unguiculata) is one of the most important legumes cultivated in many parts of the world. The diseases caused by Cowpea severe Mosaic Virus (CPSMV) and Cowpea aphid-borne Mosaic Virus (CABMV) are considered among the most important constraints on yield and quality, especially in Latin America and Africa. Here, the concept of using an RNA interference construct to silence the CPSMV proteinase cofactor gene and the CABMV coat protein gene is explored, in order to generate resistant transgenic cowpea plants. Ten cowpea transgenic lines were produced, presenting a normal phenotype and transferring the transgene to the next generation. Plants were tested for resistance to both CABMV and CPSMV by mechanical co-inoculation. Seven lines presented milder symptoms when compared to the control and three lines presented enhanced resistance to both Viruses. Northern analyses were carried out to detect the transgene-derived small interfering RNA (siRNA) in leaves and revealed no correlation between siRNA levels and Virus resistance. Additionally, in the symptomless resistant lines the resistance was homozygosis-dependent. Only homozygous plants remained uninfected while hemizygous plants presented milder symptoms.
Margaret G. Redinbaugh - One of the best experts on this subject based on the ideXlab platform.
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johnsongrass Mosaic Virus contributes to maize lethal necrosis in east africa
Plant Disease, 2017Co-Authors: Lucy R. Stewart, Margaret G. Redinbaugh, Kristen Willie, Saranga Wijeratne, Deogracious Massawe, C L Niblett, Andrew Kiggundu, Theodore AsiimweAbstract:Maize lethal necrosis (MLN), a severe Virus disease of maize, has emerged in East Africa in recent years with devastating effects on production and food security where maize is a staple subsistence crop. In extensive surveys of MLN-symptomatic plants in East Africa, sequences of Johnsongrass Mosaic Virus (JGMV) were identified in Uganda, Kenya, Rwanda, and Tanzania. The East African JGMV is distinct from previously reported isolates and infects maize, sorghum, and Johnsongrass but not wheat or oat. This isolate causes MLN in coinfection with Maize chlorotic mottle Virus (MCMV), as reported for other potyViruses, and was present in MLN-symptomatic plants in which the major East African potyVirus, Sugarcane Mosaic Virus (SCMV), was not detected. Virus titers were compared in single and coinfections by quantitative reverse transcription-polymerase chain reaction. MCMV titer increased in coinfected plants whereas SCMV, Maize dwarf Mosaic Virus, and JGMV titers were unchanged compared with single infections at...
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Response of maize (Zea mays L.) lines carrying Wsm1, Wsm2, and Wsm3 to the potyViruses Johnsongrass Mosaic Virus and Sorghum Mosaic Virus
Molecular Breeding, 2013Co-Authors: Lucy R. Stewart, Md. Ashraful Haque, Mark W. Jones, Margaret G. RedinbaughAbstract:Maize dwarf Mosaic disease is one of the most important viral diseases of maize ( Zea mays L.) throughout the world. It is caused by several Virus species in the family Potyviridae , genus PotyVirus , including Maize dwarf Mosaic Virus (MDMV), Sugarcane Mosaic Virus (SCMV), Johnsongrass Mosaic Virus (JGMV) and Sorghum Mosaic Virus (SrMV). Resistance to another member of the family Potyviridae, Wheat streak Mosaic Virus (WSMV), is conferred by three alleles ( Wsm1 , Wsm2 , Wsm3 ) in the maize inbred line Pa405, and these or closely linked genes were previously shown to confer resistance to the potyViruses MDMV and SCMV. In this study, we assessed whether Wsm alleles are linked to resistance to JGMV and SrMV. Near isogenic lines (NILs) carrying one or two of the Wsm alleles introgressed into the susceptible line Oh28 and F1 progeny from NIL × Oh28 were tested for their response to JGMV and SrMV. Our results indicate that Wsm 1 provides resistance to both JGMV and SrMV in a dose-dependent manner. Wsm2 and Wsm3 each provide limited resistance, and combining Wsm alleles enhances that resistance.
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the mdm1 locus and maize resistance to maize dwarf Mosaic Virus
Plant Disease, 2007Co-Authors: M W Jones, Margaret G. Redinbaugh, R LouieAbstract:Jones, M. W., Redinbaugh, M. G., and Louie, R. 2007. The Mdm1 locus and maize resistance to Maize dwarf Mosaic Virus. Plant Dis. 91:185-190. Previously, Mdm1, a gene controlling resistance to Maize dwarf Mosaic Virus (MDMV), was identified in the inbred line Pa405. The gene was tightly linked to the restriction fragment length polymorphism marker umc85 on the short arm of chromosome 6. This chromosomal region is also the location of resistance genes to two other Viruses in the family Potyviridae, Sugarcane Mosaic Virus (SCMV) and Wheat streak Mosaic Virus (WSMV). A diverse collection of 115 maize inbred lines was evaluated for resistance to MDMV and SCMV, and for MDMV resistance loci on chromosome 6S. Forty-six resistant inbred lines were crossed to three MDMVsusceptible inbred lines to develop F2 populations. The F2 populations were inoculated with MDMV and scored for infection and symptom type. Environmental factors influenced both the rate and type of symptom development. Bulked segregant analysis of each F2 population indicated that, in 42 of 43 MDMV-resistant lines, chromosome 6S markers found in the resistant parent also were present in the bulked resistant but not the susceptible tissue. Markers previously associated with resistance to both SCMV and WSMV on chromosome 3 and to WSMV on chromosome 10 were associated with resistance in nine and seven of the F2 populations, respectively. These data suggest that Mdm1 or closely linked genes on chromosome 6S are associated with MDMV resistance in most germplasm, but that other loci also may affect resistance.
