The Experts below are selected from a list of 16413 Experts worldwide ranked by ideXlab platform
Peter X - One of the best experts on this subject based on the ideXlab platform.
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gold and gold silver alloy nanoparticles enhance the Myogenic Differentiation of myoblasts through p38 mapk signaling pathway and promote in vivo skeletal muscle regeneration
Biomaterials, 2018Co-Authors: Juan Ge, Peter X, Mi Chen, Min WangAbstract:Abstract Under the severe trauma condition, the skeletal muscles regeneration process is inhibited by forming fibrous scar tissues. Understanding the interaction between bioactive nanomaterials and myoblasts perhaps has important effect on the enhanced skeletal muscle tissue regeneration. Herein, we investigate the effect of monodispersed gold and gold-silver nanoparticles (AuNPs and Au-AgNPs) on the proliferation, Myogenic Differentiation and associated molecular mechanism of myoblasts (C2C12), as well as the in vivo skeletal muscle tissue regeneration. Our results showed that AuNPs and Au-AgNPs could support myoblast attachment and proliferation with negligible cytotoxicity. Under various incubation conditions (normal and Differentiation medium), AuNPs and Au-AuNPs significantly enhanced the Myogenic Differentiation of myoblasts by upregulating the expressions of myosin heavy chain (MHC) protein and Myogenic genes (MyoD, MyoG and Tnnt-1). The further analysis demonstrated that AuNPs and Au-AgNPs could activate the p38α mitogen-activated protein kinase pathway (p38α MAPK) signaling pathway and enhance the Myogenic Differentiation. Additionally, the AuNPs and Au-AgNPs significantly promote the in vivo skeletal muscle regeneration in a tibialis anterior muscle defect model of rat. This study may provide a nanomaterials-based strategy to improve the skeletal muscle repair and regeneration.
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biomimetic elastomeric conductive and biodegradable polycitrate based nanocomposites for guiding Myogenic Differentiation and skeletal muscle regeneration
Biomaterials, 2018Co-Authors: Peter X, Bo LeiAbstract:Artificial muscle-like biomaterials have gained tremendous interests owing to their broad applications in regenerative medicine, wearable devices, bioelectronics and artificial intelligence. Unfortunately, key challenges are still existed for current materials, including biomimetic viscoelasticity, biocompatibility and biodegradation, multifunctionality. Herein, for the first time, we develop highly elastomeric, conductive and biodegradable poly (citric acid-octanediol-polyethylene glycol)(PCE)-graphene (PCEG) nanocomposites, and demonstrate their applications in Myogenic Differentiation and guiding skeletal muscle tissue regeneration. In PCEG nanocomposites, PCE provides the biomimetic elastomeric behavior, and the addition of reduced graphene oxide (RGO) endows the enhanced mechanical strength and conductivity. The highly elastomeric behavior, significantly enhanced modulus (400%–800%), strength (200%–300%) of PCEG nanocomposites with controlled biodegradability and electrochemical conductivity were achieved. The myoblasts proliferation and Myogenic Differentiation were significantly improved by PCEG nanocomposite. Significantly high in vivo biocompatibility of PCEG nanocomposites was observed when implanted in the subcutaneous tissue for 4 weeks in rats. PCEG nanocomposites could significantly enhance the muscle fibers and blood vessels formation in vivo in a skeletal muscle lesion model of rat. This study may provide a novel strategy to develop multifunctional elastomeric nanocomposites with high biocompatibility for potential soft tissue regeneration and stretchable bioelectronic devices.
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biocompatible elastic conductive films significantly enhanced Myogenic Differentiation of myoblast for skeletal muscle regeneration
Biomacromolecules, 2017Co-Authors: Ruonan Dong, Xin Zhao, Baolin Guo, Peter XAbstract:The key factor in skeletal muscle tissue engineering is regeneration of the functional skeletal muscles. Materials that could promote the myoblast proliferation and Myogenic Differentiation are promising candidates in skeletal muscle tissue engineering. Herein, we developed an elastic conductive poly(ethylene glycol)-co-poly(glycerol sebacate) (PEGS) grafted aniline pentamer (AP) copolymer that could promote the formation of myotubes by differentiating the C2C12 myoblast cells. The results of hydration behavior and water contact angle suggested that by adjusting the poly(ethylene glycol) (PEG) and AP content, this film showed a proper surface hydrophilicity for cell attachment. Additionally, these films showed tunable conductivity and mechanical properties that can be altered by changing the AP content. The maximum conductivity of the films was 1.84 × 10–4 S/cm and the Young’s modulus of these films ranged from 14.58 ± 1.35 MPa to 24.62 ± 0.61 MPa. Our findings indicate that the PEGS-AP films promote the ...
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stretchable degradable and electroactive shape memory copolymers with tunable recovery temperature enhance Myogenic Differentiation
Acta Biomaterialia, 2016Co-Authors: Zexing Deng, Ruonan Dong, Xin Zhao, Yi Guo, Baolin Guo, Peter XAbstract:Abstract Development of flexible degradable electroactive shape memory polymers (ESMPs) with tunable switching temperature (around body temperature) for tissue engineering is still a challenge. Here we designed and synthesized a series of shape memory copolymers with electroactivity, super stretchability and tunable recovery temperature based on poly(e-caprolactone) (PCL) with different molecular weight and conductive amino capped aniline trimer, and demonstrated their potential to enhance Myogenic Differentiation from C2C12 myoblast cells. We characterized the copolymers by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), cyclic voltammetry (CV), ultraviolet–visible spectroscopy (UV–vis), differential scanning calorimetry (DSC), shape memory test, tensile test and in vitro enzymatic degradation study. The electroactive biodegradable shape memory copolymers showed great elasticity, tunable recovery temperature around 37 °C, and good shape memory properties. Furthermore, proliferation and Differentiation of C2C12 myoblasts were investigated on electroactive copolymers films, and they greatly enhanced the proliferation, myotube formation and related Myogenic Differentiation genes expression of C2C12 myoblasts compared to the pure PCL with molecular weight of 80,000. Our study suggests that these electroactive, highly stretchable, biodegradable shape memory polymers with tunable recovery temperature near the body temperature have great potential in skeletal muscle tissue engineering application. Statement of Significance Conducting polymers can regulate cell behavior such cell adhesion, proliferation, and Differentiation with or without electrical stimulation. Therefore, they have great potential for electrical signal sensitive tissue regeneration. Although conducting biomaterials with degradability have been developed, highly stretchable and electroactive degradable copolymers for soft tissue engineering have been rarely reported. On the other hand, shape memory polymers (SMPs) have been widely used in biomedical fields. However, SMPs based on polyesters usually are biologically inert. This work reported the design of super stretchable electroactive degradable SMPs based on polycaprolactone and aniline trimer with tunable recovery temperature around body temperature. These flexible electroactive SMPs facilitated the proliferation and Differentiation of C2C12 myoblast cells compared with polycaprolactone, indicating that they are excellent scaffolding biomaterials in tissue engineering to repair skeletal muscle and possibly other tissues.
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Myogenic Differentiation of human bone marrow mesenchymal stem cells on a 3d nano fibrous scaffold for bladder tissue engineering
Biomaterials, 2010Co-Authors: Peter X, Hong Tian, Shantaram Bharadwaj, Anthony Atala, Yuanyuan ZhangAbstract:Abstract Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and Differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-β1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce Myogenic Differentiation of BMSC. A highly porous poly- l -lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained Myogenic Differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo . Our results demonstrate that Myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.
Yuanyuan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Myogenic Differentiation of human bone marrow mesenchymal stem cells on a 3d nano fibrous scaffold for bladder tissue engineering
Biomaterials, 2010Co-Authors: Peter X, Hong Tian, Shantaram Bharadwaj, Anthony Atala, Yuanyuan ZhangAbstract:Abstract Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and Differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-β1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce Myogenic Differentiation of BMSC. A highly porous poly- l -lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained Myogenic Differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo . Our results demonstrate that Myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.
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Myogenic Differentiation of human bone marrow mesenchymal stem cells on a 3d nano fibrous scaffold for bladder tissue engineering
Biomaterials, 2010Co-Authors: Hong Tian, Peter X, Shantaram Bharadwaj, Anthony Atala, Yan Liu, Yuanyuan ZhangAbstract:Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and Differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-beta1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce Myogenic Differentiation of BMSC. A highly porous poly-l-lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained Myogenic Differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo. Our results demonstrate that Myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.
Hong Tian - One of the best experts on this subject based on the ideXlab platform.
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Myogenic Differentiation of human bone marrow mesenchymal stem cells on a 3d nano fibrous scaffold for bladder tissue engineering
Biomaterials, 2010Co-Authors: Peter X, Hong Tian, Shantaram Bharadwaj, Anthony Atala, Yuanyuan ZhangAbstract:Abstract Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and Differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-β1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce Myogenic Differentiation of BMSC. A highly porous poly- l -lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained Myogenic Differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo . Our results demonstrate that Myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.
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Myogenic Differentiation of human bone marrow mesenchymal stem cells on a 3d nano fibrous scaffold for bladder tissue engineering
Biomaterials, 2010Co-Authors: Hong Tian, Peter X, Shantaram Bharadwaj, Anthony Atala, Yan Liu, Yuanyuan ZhangAbstract:Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and Differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-beta1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce Myogenic Differentiation of BMSC. A highly porous poly-l-lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained Myogenic Differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo. Our results demonstrate that Myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.
Jason D. White - One of the best experts on this subject based on the ideXlab platform.
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Hyaluronan synthesis and myogenesis; a requirement for hyaluronan synthesis during Myogenic Differentiation independent of pericellular matrix formation
The Journal of biological chemistry, 2013Co-Authors: Liam C Hunt, Christopher Gorman, Christopher Kintakas, Daniel R. Mcculloch, Eleanor J. Mackie, Jason D. WhiteAbstract:Abstract Exogenous hyaluronan is known to alter muscle precursor cell proliferation, migration, and Differentiation, ultimately inhibiting myogenesis in vitro. The aim of the current study was to investigate the role of endogenous hyaluronan synthesis during myogenesis. In quantitative PCR studies, the genes responsible for synthesizing hyaluronan were found to be differentially regulated during muscle growth, repair, and pathology. Although all Has genes (Has1, Has2, and Has3) were differentially regulated in these models, only Has2 gene expression consistently associated with Myogenic Differentiation. During Myogenic Differentiation in vitro, Has2 was the most highly expressed of the synthases and increased after induction of Differentiation. To test whether this association between Has2 expression and myogenesis relates to a role for Has2 in myoblast Differentiation and fusion, C2C12 myoblasts were depleted of Has2 by siRNA and induced to differentiate. Depletion of Has2 inhibited Differentiation and caused a loss of cell-associated hyaluronan and the hyaluronan-dependent pericellular matrix. The inhibition of Differentiation caused by loss of hyaluronan was confirmed with the hyaluronan synthesis inhibitor 4-methylumbelliferone. In hyaluronan synthesis-blocked cultures, restoration of the pericellular matrix could be achieved through the addition of exogenous hyaluronan and the proteoglycan versican, but this was not sufficient to restore Differentiation to control levels. These data indicate that intrinsic hyaluronan synthesis is necessary for myoblasts to differentiate and form syncytial muscle cells, but the hyaluronan-dependent pericellular matrix is not sufficient to support Differentiation alone; additional hyaluronan-dependent cell functions that are yet unknown may be required for Myogenic Differentiation.
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caspase 3 Myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of Myogenic Differentiation
Skeletal Muscle, 2011Co-Authors: Liam C Hunt, Jason D. White, Aradhana Upadhyay, Jalal A Jazayeri, Elizabeth M TudorAbstract:Leukemia inhibitory factor (LIF) is known to inhibit Myogenic Differentiation as well as to inhibit apoptosis and caspase-3 activation in non-differentiating myoblasts. In addition caspase-3 activity is required for Myogenic Differentiation. Therefore the aim of this study was to further investigate mechanisms of the Differentiation suppressing effect of LIF in particular the possibility of a caspase-3 mediated inhibition of Differentiation. LIF dependent inhibition of Differentiation appeared to involve several mechanisms. Differentiating myoblasts that were exposed to LIF displayed increased transcripts for c-fos. Transcripts for the cell cycle inhibitor p21 as well as muscle regulatory factors myoD and myogenin were decreased with LIF exposure. However, LIF did not directly induce a proliferative effect under Differentiation conditions, but did prevent the proportion of myoblasts that were proliferating from decreasing as Differentiation proceeded. LIF stimulation decreased the percentage of cells positive for active caspase-3 occurring during Differentiation. Both the effect of LIF inhibiting caspase-3 activation and Differentiation appeared dependent on mitogen activated protein kinase and extracellular signal regulated kinase kinase (MEK) signalling. The role of LIF in Myogenic Differentiation was further refined to demonstrate that myoblasts are unlikely to secrete LIF endogenously. Altogether this study provides a more comprehensive view of the role of LIF in Myogenic Differentiation including LIF and receptor regulation in myoblasts and myotubes, mechanisms of inhibition of Differentiation and the link between caspase-3 activation, apoptosis and Myogenic Differentiation.
Liam C Hunt - One of the best experts on this subject based on the ideXlab platform.
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Hyaluronan synthesis and myogenesis; a requirement for hyaluronan synthesis during Myogenic Differentiation independent of pericellular matrix formation
The Journal of biological chemistry, 2013Co-Authors: Liam C Hunt, Christopher Gorman, Christopher Kintakas, Daniel R. Mcculloch, Eleanor J. Mackie, Jason D. WhiteAbstract:Abstract Exogenous hyaluronan is known to alter muscle precursor cell proliferation, migration, and Differentiation, ultimately inhibiting myogenesis in vitro. The aim of the current study was to investigate the role of endogenous hyaluronan synthesis during myogenesis. In quantitative PCR studies, the genes responsible for synthesizing hyaluronan were found to be differentially regulated during muscle growth, repair, and pathology. Although all Has genes (Has1, Has2, and Has3) were differentially regulated in these models, only Has2 gene expression consistently associated with Myogenic Differentiation. During Myogenic Differentiation in vitro, Has2 was the most highly expressed of the synthases and increased after induction of Differentiation. To test whether this association between Has2 expression and myogenesis relates to a role for Has2 in myoblast Differentiation and fusion, C2C12 myoblasts were depleted of Has2 by siRNA and induced to differentiate. Depletion of Has2 inhibited Differentiation and caused a loss of cell-associated hyaluronan and the hyaluronan-dependent pericellular matrix. The inhibition of Differentiation caused by loss of hyaluronan was confirmed with the hyaluronan synthesis inhibitor 4-methylumbelliferone. In hyaluronan synthesis-blocked cultures, restoration of the pericellular matrix could be achieved through the addition of exogenous hyaluronan and the proteoglycan versican, but this was not sufficient to restore Differentiation to control levels. These data indicate that intrinsic hyaluronan synthesis is necessary for myoblasts to differentiate and form syncytial muscle cells, but the hyaluronan-dependent pericellular matrix is not sufficient to support Differentiation alone; additional hyaluronan-dependent cell functions that are yet unknown may be required for Myogenic Differentiation.
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caspase 3 Myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of Myogenic Differentiation
Skeletal Muscle, 2011Co-Authors: Liam C Hunt, Jason D. White, Aradhana Upadhyay, Jalal A Jazayeri, Elizabeth M TudorAbstract:Leukemia inhibitory factor (LIF) is known to inhibit Myogenic Differentiation as well as to inhibit apoptosis and caspase-3 activation in non-differentiating myoblasts. In addition caspase-3 activity is required for Myogenic Differentiation. Therefore the aim of this study was to further investigate mechanisms of the Differentiation suppressing effect of LIF in particular the possibility of a caspase-3 mediated inhibition of Differentiation. LIF dependent inhibition of Differentiation appeared to involve several mechanisms. Differentiating myoblasts that were exposed to LIF displayed increased transcripts for c-fos. Transcripts for the cell cycle inhibitor p21 as well as muscle regulatory factors myoD and myogenin were decreased with LIF exposure. However, LIF did not directly induce a proliferative effect under Differentiation conditions, but did prevent the proportion of myoblasts that were proliferating from decreasing as Differentiation proceeded. LIF stimulation decreased the percentage of cells positive for active caspase-3 occurring during Differentiation. Both the effect of LIF inhibiting caspase-3 activation and Differentiation appeared dependent on mitogen activated protein kinase and extracellular signal regulated kinase kinase (MEK) signalling. The role of LIF in Myogenic Differentiation was further refined to demonstrate that myoblasts are unlikely to secrete LIF endogenously. Altogether this study provides a more comprehensive view of the role of LIF in Myogenic Differentiation including LIF and receptor regulation in myoblasts and myotubes, mechanisms of inhibition of Differentiation and the link between caspase-3 activation, apoptosis and Myogenic Differentiation.
