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Alsaid A Haffor - One of the best experts on this subject based on the ideXlab platform.
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effect of commiphora molmol on leukocytes proliferation in relation to histological alterations before and during healing from injury
Saudi Journal of Biological Sciences, 2010Co-Authors: Alsaid A HafforAbstract:Myrrh, Commiphora molmol has been used as anti-inflammatory and wound healing commercial product. Leukocyte count had been reliable indicator for clinicians to monitor progress of healing for their patients. We hypothesized that Myrrh supplement participate in the activation of leukocyte proliferation pathway prior and post skin injury and gastric ulcer. The purpose of the present study was to examine long-term effect of Myrrh on leukocytes proliferation before injury and during different stages of healing. Results showed that all types of leukocytes were significantly (p < 0.05) higher in the Myrrh-treated groups before and during healing. The pretreatment with Myrrh offered a time-dependent rise in leukocytes proliferation. Microscopic examination of blood smear from Myrrh-treated rats with skin injury, showed an elevated count of middle-sized lymphocytes and neutrophils that were characterized with well-defined nuclear lobules and rich-granules cytoplasm. Furthermore, the microscopic examinations of the spleen and lymph nodes of Myrrh-treated rats with skin injury, showed an increased thickness of lymphatic sheath around the arterioles in the white pulp that was associated with high density of the medium-sized lymphocytes in the secondary lymphoid follicles in the lymph nodes with engorged sinusoids. As Myrrh enhanced leukocytes proliferation before injury, it can be concluded that Myrrh posse’s antigenic-driven responses and that indicated some foreignness or toxicity of some constituents of Myrrh. Because Myrrh helped to maintain the relative rise of leukocytes counts throughout healing period and that implied it activated late steps of both proliferation and differentiation pathways for all types of leukocytes during effective phase of the specific immune responses.
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effect of Myrrh commiphora molmol on leukocyte levels before and during healing from gastric ulcer or skin injury
Journal of Immunotoxicology, 2010Co-Authors: Alsaid A HafforAbstract:Myrrh (Commiphora molmol) has been widely used as an anti-inflammatory and wound healing commercial product. As white blood cell (WBC)/leukocyte counts have been used as an indicator by clinicians to monitor progress of healing in patients, the purpose of this study was to examine effects of Myrrh supplementation on blood WBC numbers before an injury and during healing. Male rats (7-8-wk-of-age) were randomly assigned to four groups. Group 1 (SIM) served as "skin injury treated + Myrrh treatment (500 mg/kg/day)," Group 2 (SI) as "skin injury alone", Group 3 (GUM) as "gastric ulcer treated + Myrrh treatment", and Group 4 (GU) as gastric ulcer only. Myrrh treatments (via drinking water) began 4 wk before induction of injury and continued for a 2 wk period post-injury. Baseline values for each WBC type were recorded before start of the Myrrh treatments. Counts were performed again on Day 1 of the 5th wk (1-2 hr before injury) and post-injury on Days 4 and 7 of the 5th wk, and a final time on Day 4 of the 6th wk. Results showed that levels of all WBC types were significantly (P < 0.05) elevated before either injury in Myrrh-treated rats (Groups 1 and 3) as compared with levels in rats in Groups 2 and 4. At all timepoints, there were neither significant differences between the values seen with rats in Groups 1 and 3, nor between those in Groups 2 and 4. Treatment with Myrrh also induced an initial increase in WBC levels that persisted through the post-injury healing period. Levels of most cell types only increased in the Group 2 and 4 rats once the injury was induced, but then declined over the healing period. Since Myrrh enhanced WBC levels before injury, we conclude that Myrrh likely contains substances that could induce an apparent antigen-driven response. As the Myrrh also helped maintain elevated WBC levels throughout the healing period, this implied it was also able to induce maturation/differentiation/activation of both myeloid and lymphoid cell types during the effector phase of the immune responses involved in wound healing.
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effect of Myrrh commiphora molmol on leukocyte levels before and during healing from gastric ulcer or skin injury
Journal of Immunotoxicology, 2010Co-Authors: Alsaid A HafforAbstract:Myrrh (Commiphora molmol) has been widely used as an anti-inflammatory and wound healing commercial product. As white blood cell (WBC)/leukocyte counts have been used as an indicator by clinicians to monitor progress of healing in patients, the purpose of this study was to examine effects of Myrrh supplementation on blood WBC numbers before an injury and during healing. Male rats (7–8-wk-of-age) were randomly assigned to four groups. Group 1 (SIM) served as “skin injury treated + Myrrh treatment (500 mg/kg/day),” Group 2 (SI) as “skin injury alone”, Group 3 (GUM) as “gastric ulcer treated + Myrrh treatment”, and Group 4 (GU) as gastric ulcer only. Myrrh treatments (via drinking water) began 4 wk before induction of injury and continued for a 2 wk period post-injury. Baseline values for each WBC type were recorded before start of the Myrrh treatments. Counts were performed again on Day 1 of the 5th wk (1–2 hr before injury) and post-injury on Days 4 and 7 of the 5th wk, and a final time on Day 4 of the 6th...
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effect of Myrrh commiphora molmol on leukocyte levels before and during healing from gastric ulcer or skin injury
Journal of Immunotoxicology, 2010Co-Authors: Alsaid A HafforAbstract:Myrrh (Commiphora molmol) has been widely used as an anti-inflammatory and wound healing commercial product. As white blood cell (WBC)/leukocyte counts have been used as an indicator by clinicians ...
C Vissiennon - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory and barrier stabilising effects of Myrrh coffee charcoal and chamomile flower extract in a co culture cell model of the intestinal mucosa
Biomolecules, 2020Co-Authors: Laura Weber, Katrin Kuck, Guido Jurgenliemk, Jorg Heilmann, Bartosz Lipowicz, C VissiennonAbstract:Recent clinical evidence suggests the efficacy of a traditional herbal medicinal product containing Myrrh (Commiphora molmol Engl.), coffee charcoal (Coffea arabica L.) and chamomile flower dry extract (Matricaria chamomilla L.) in the therapy of inflammatory bowel diseases (IBD). However, the mechanisms of action in this context have not been entirely elucidated. The present study aimed to evaluate the effects of Myrrh, coffee charcoal and chamomile flower extract on the inflammatory cross talk between immune and intestinal epithelial cells together with the resulting intestinal barrier disorders. A complex co-culture cell model consisting of intestinal epithelial cell (IEC) monolayers (Caco-2, HT29-MTX-E12) and macrophages (THP-1) was established for the simultaneous investigation of these two IBD characteristics. The lipopolysaccharide (LPS) activation of the macrophages led to a pro-inflammatory mediator release and thereby an inflammatory stimulation of IECs with chemokine release and reduced barrier function. The effects of the individual plant extracts and a ternary combination on inflammatory mediator release (IL-6, TNF, IL-8, MCP-1, PGE2) was quantified by ELISA. The transepithelial electrical resistance (TEER) of IEC monolayers was measured to evaluate the effects on the barrier function. Budesonide served as a positive control. All three plant extracts exhibited anti-inflammatory properties via the inhibition of the inflammatory mediator release to a varying extent. An intestinal barrier stabilising effect was observed for Myrrh and coffee charcoal. Myrrh exerted the most distinct pharmacological activity. Dose reducing and synergistic interactions emerged within the threefold combination. Thus, our results provide a mechanistic basis for the use of the herbal combination of Myrrh, coffee charcoal and chamomile flower extract in IBD treatment and underline the potential benefits of the phytotherapeutic multi-component/multi-target approach in this complex pathogenesis.
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synergistic interactions of chamomile flower Myrrh and coffee charcoal in inhibiting pro inflammatory chemokine release from activated human macrophages
Synergy, 2017Co-Authors: C Vissiennon, K H Goos, K Nieber, D Hammoud, Jurgen ArnholdAbstract:Abstract Background The herbal medicinal product Myrrhinil-Intest®, a combination of Myrrh, chamomile flower extract and coffee charcoal is used for the maintenance therapy of inflammatory bowel disease. In vitro studies revealed that the herbal components influence chemokine signaling of human macrophages as part of an anti-inflammatory mechanism. However, the occurrence of synergistic effects remains unexplored. Aim The present study aims to investigate the synergistic effect of dual combinations of the plant extracts on the pro-inflammatory chemokine (CXCL13) release from activated human macrophages. Methods The single effect of Myrrh, chamomile flower and coffee charcoal extract on CXCL13 release from lipopolysaccharide stimulated human macrophages was investigated using ELISA and IC50 values were determined. Budesonide served as positive control. To characterize the combined effect, IC50 values were used to prepare combinations of two plant extracts in different proportions to each other (3:5; 1:1; 5:3). Interpretation of the data was based on isobologram analysis and calculation of a combination index (CI). Results LPS-induced CXCL13 release from human macrophages was inhibited after treatment with Myrrh (IC50 = 19 μg/ml), chamomile flower (IC50 = 82 μg/ml) and coffee charcoal (IC50 = 106 μg/ml) whereby the extent of inhibition was comparable to budesonide. All combinations of two plant extracts resulted in synergistic effects with varying magnitude (CI from 0.82 to 0.42). The combination of Myrrh and coffee charcoal (ratio of 3:5) exhibited the strongest synergistic effect (CI = 0.42). Increasing amounts of coffee charcoal resulted in increased synergistic activity. Conclusion Synergistic effects between all plant components contribute to the anti-inflammatory activity of all dual combinations of the plant components and support the composition of the herbal combination.
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chamomile flower Myrrh and coffee charcoal components of a traditional herbal medicinal product diminish proinflammatory activation in human macrophages
Planta Medica, 2016Co-Authors: C Vissiennon, K H Goos, K Nieber, D Hammoud, Steffen Rodewald, Karin Fester, Jurgen ArnholdAbstract:A traditional herbal medicinal product, containing Myrrh, chamomile flower, and coffee charcoal, has been used in Germany for the relief of gastrointestinal complaints for decades. Clinical studies suggest its use in the maintenance therapy of inflammatory bowel disease. However, the pharmacological mechanisms underlying the clinical effects are not yet fully understood.The present study aims to elucidate immunopharmacological activities of Myrrh, chamomile flower, and coffee charcoal by studying the influence of each plant extract on gene expression and protein release of activated human macrophages.The plant extracts effect on gene and protein expression of activated human monocyte-derived macrophages was investigated by microarray gene expression analysis and assessment of the release of pro- and anti-inflammatory mediators (TNFα, chemokine CXCL13, and interleukin-10) using an ELISA test system.The extracts of Myrrh, chamomile flower, and coffee charcoal influenced gene expression of activated human macrophages within the cytokine/chemokine signaling pathway. Particularly, chemokine gene expression was suppressed. Subsequently, the production of CXCL13 and, to a minor extent, cytokine TNFα was inhibited by all herbal extracts. Chamomile flower and coffee charcoal extracts enhanced interleukin-10 release from activated macrophages. The observed effects on protein release were comparable to the effect of budesonide, which decreased TNFα and CXCL13 and enhanced interleukin-10 release.The components of the herbal medicinal product influence the activity of activated human macrophages on both gene and protein level. The induced alterations within chemokine/cytokine signaling could contribute to a positive effect on the immunological homeostasis, which is disturbed in patients with chronic intestinal inflammation.
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antispasmodic effects of Myrrh due to calcium antagonistic effects in inflamed rat small intestinal preparations
Planta Medica, 2015Co-Authors: C Vissiennon, K H Goos, Ole Goos, K NieberAbstract:Myrrh is the oleo-gum resin of mainly Commiphora molmol and as a powdered substance, one compound in the traditional medicinal product Myrrhinil-Intest®, which has been used for the treatment of unspecific, inflammatory intestinal disorders. The aim of the present study was to evaluate the antispasmodic effect of Myrrh under healthy and inflamed conditions, and to evaluate a calcium-antagonistic effect as a possible mode of action. Therefore, an ethanolic Myrrh extract was tested for its effects on muscle tone and acetylcholine-induced contractions in untreated and inflamed rat ileum/jejunum preparations. Inflammation was experimentally induced by 2,4,6-trinitrobenzene sulfonic acid (10 mM, 30 min). Additionally, the effect of the calcium channel agonist Bay K8644 in the presence of varying Myrrh extract concentrations was examined. Myrrh extract (0.99 mg/mL) suppressed the acetylcholine-induced contraction down to 25.8 % in untreated and 15.2 % in inflamed preparations. Myrrh extract (0.15; 0.25 and 0.35 mg/mL) induced a concentration-dependent rightward shift of the Bay K8644 concentration-response curve in untreated and inflamed preparations with a significant EC50 shift. Schild analysis resulted in a pA2 value of 0.93 for untreated preparations. Increasing Myrrh extract concentrations induced a concentration-dependent decrease of the agonistic maximum effect in untreated and inflamed preparations down to 15.8 % and 25.8 %, respectively, for the highest concentration leading to a pD2 value of 0.58. Myrrh extract reduced intestinal muscle tone and acetylcholine-induced contraction of untreated and inflamed ileum/jejunum preparations based on dual calcium antagonism characterized by a right shift of the agonistic dose-response curve and a depression of the maximum effect. The resulting reduction of intestinal motility and spasmolytic effects provide a rationale for the symptom treatment of intestinal disorders such as irritable bowel syndrome.
Slavisa Stankovic - One of the best experts on this subject based on the ideXlab platform.
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frankincense and Myrrh essential oils and burn incense fume against micro inhabitants of sacral ambients wisdom of the ancients
Journal of Ethnopharmacology, 2018Co-Authors: Milica Ljaljevic Grbic, Nikola Unkovic, Ivica Dimkic, Peđa Janackovic, Milan Gavrilovic, Olja Stanojevic, Milos Stupar, Ljubodrag Vujisic, Aleksa Jelikic, Slavisa StankovicAbstract:Abstract Ethnopharmacological relevance Essential oils obtained from resins of Boswellia carteri Birdw. and Commiphora Myrrha (Nees) Engl., commonly known as frankincense and true Myrrh respectively, have been used extensively since 2800 BCE for the treatment of skin sores, wounds, teeth, inflammation, and urinary tract diseases in traditional medicine; for preparation of mummification balms and unguents; and also as incense and perfumes. Since ancient times, burning of frankincense and Myrrh in places of worship for spiritual purposes and contemplation (a ubiquitous practice across various religions) had hygienic functions, to refine the smell and reduce contagion by purifying the indoor air. Aim of the study The general purpose of the study was to assess the in vitro antimicrobial potential of the liquid and vapour phases of B. carteri and C. Myrrha essential oils and burn incense, as well as to test the effectiveness of their in situ application to cleanse microbially-contaminated air within the ambient of an investigated 17th-century church. Materials and methods The chemical composition of B. carteri and C. Myrrha essential oils, obtained by hydrodistillation of frankincense and true Myrrh oleo gum resins was determined using GC/MS, and antimicrobial properties of their liquid and vapour phases were assessed by the broth microdilution and microatmosphere diffusion methods. Chemical analysis of burn incense fume obtained using bottle gas washing with dichloromethane as a solvent was performed by GC/MS, while its antimicrobial activity was evaluated using a modified microatmosphere diffusion method to evaluate germination inhibition for fungi and CFU count reduction for bacteria. The in situ antimicrobial activity of B. carteri burn incense and essential oil vapour phase was assessed in the sealed nave and diaconicon of the church, respectively. Results The dominant compounds of B. carteri EO were α-pinene (38.41%) and myrcene (15.21%), while C. Myrrha EO was characterized by high content of furanoeudesma-1,3-diene (17.65%), followed by curzerene (12.97%), β-elemene (12.70%), and germacrene B (12.15%). Burn incense fume and soot had α-pinene (68.6%) and incensole (28.6%) as the most dominant compounds, respectively. In vitro antimicrobial assays demonstrated high bacterial and fungal sensitivity to the liquid and vapour phases of EOs, and burn incense fume. In situ application of B. carteri EO vapour and incense fume resulted in reduction of air-borne viable microbial counts by up to 45.39 ± 2.83% for fungi and 67.56 ± 3.12% for bacteria (EO); and by up to 80.43 ± 2.07% for fungi and 91.43 ± 1.26% for bacteria (incense fume). Conclusions The antimicrobial properties of essential oil derived from frankincense, a compound with well-known traditional use, showed that it possesses a clear potential as a natural antimicrobial agent. Moreover, the results suggest possible application of B. carteri EO vapour and incense fume as occasional air purifiers in sacral ambients, apart from daily church rituals.
Osama Salama - One of the best experts on this subject based on the ideXlab platform.
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protection by turmeric and Myrrh against liver oxidative damage and genotoxicity induced by lead acetate in mice
Basic & Clinical Pharmacology & Toxicology, 2006Co-Authors: Ibrahim M Elashmawy, Khalid M Ashry, Abeer F Elnahas, Osama SalamaAbstract:Abstract: The effects of lead acetate in the diet (0.5% w/w) on reduced GSH, activity of phase II metabolizing enzyme glutathione S-transferase (GST), lipid peroxidation in liver homogenate and bone marrow chromosomes of mice simultaneously supplemented with powdered turmeric and Myrrh for 8 weeks were investigated. Five groups of Swiss male albino mice, each of 30 mice, the first group received a basal diet and served as negative control, the second group received basal diet supplemented with lead acetate only and served as positive control. The other three groups received basal diet supplemented with lead acetate and 1% or 5% turmeric powder and 1% Myrrh powder, respectively. Results revealed a significant decrease in the amount of GSH in all treated groups compared with negative control. Also, the activity of GSH S-transferase was significantly decreased in positive control compared with other groups. However, co-administration of the protective plants resulted in a significant increase in the activity of GST compared with both positive and negative control groups. Furthermore, lipid peroxidation was significantly increased in positive control alone, while cotreatment with the protective plants resulted in reduction in the level of lipid peroxidation by 31% and 49% in mice receiving 1% and 5% turmeric powder respectively and 45% in 1% Myrrh treated when compared with their respective positive control group. Lead genotoxicity was confirmed through significant reduction in the number of dividing cells, increased total number of aberrant cells and increased frequency of chromosomal aberrations. Simultaneous treatment with these plants significantly reduced the genotoxicity induced by lead administration and the powerful protection was observed with 5% powdered turmeric. It may be concluded that turmeric and Myrrh are useful herbal remedies, especially for controlling oxidative damages and genotoxicity induced by lead acetate intoxication.
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components therapeutic value and uses of Myrrh
Die Pharmazie, 2003Co-Authors: El Sayed El H Ashry, N Rashed, Osama Salama, Amal SalehAbstract:Occurrence, constituents and medicinal use of Myrrh, obtained from the stem of different Commiphora species are reviewed. The constituents of the volatile oil, the resin and the gum are outlined in detail. Myrrh has considerable antimicrobial activity and is medicinally used in a variety of diseases.
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preliminary study of therapeutic efficacy of a new fasciolicidal drug derived from commiphora molmol Myrrh
American Journal of Tropical Medicine and Hygiene, 2001Co-Authors: Ahmed Massoud, El S Sisi, Osama SalamaAbstract:Myrrh (from the stem of the Commiphora molmol tree) is an oleo gum resin that may prove efficacious for the treatment of fascioliasis. We studied 7 patients who were passing Fasciola eggs in their stools and treated them with Myrrh. The drug (a formulation consisting of 8 parts of resin and 3.5 parts of volatile oils, all extracted from Myrrh) was given in a dose of 12 mg/kg per day for 6 consecutive days in the morning on an empty stomach. Patients were followed for 3 months. The therapy proved to be effective, with pronounced improvement of the general condition and amelioration of all symptoms and signs. A dramatic drop in the egg count was detected at the end of treatment. Eggs were no longer detectable in the feces 3 weeks after treatment and after a follow-up period of 3 months. High eosinophilic counts, elevated liver enzymes, and Fasciola antibody titers returned to nearly normal. No signs of toxicity or adverse effects were observed. We conclude that the formulation of Myrrh is safe, well tolerated, and effective for treating fascioliasis.
Andrea Scorciapino - One of the best experts on this subject based on the ideXlab platform.
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chemical composition of the essential oil and supercritical co2 extract of commiphora Myrrha nees engl and of acorus calamus l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Andrea ScorciapinoAbstract:Volatile concentrates from the oleo-gum resin of Commiphora Myrrha (Nees) Engl. and from the rhizomes of Acorus calamus were isolated by supercritical extraction with carbon dioxide. The volatile oil of Myrrh was obtained at 9.0 MPa and 50 °C and at a CO2 flow of 1.5 kg/h. Acorus calamus was extracted at 9.0 MPa and 45 °C and at a CO2 flow of 1.6 kg/h. In both cases, an oil devoid of cuticular waxes was obtained with a single depressurization stage. The SFE Myrrh oil had a yield, Y, of 3.2%. Its main components, identified and quantified by GC/MS, were furanoeudesma-1,3-diene, 34.9%; lindestrene, 12.9%; curzerene, 8.5%; and germacrone, 5.8%. The essential oils from the same starting material by hydrodistillation, HD, (Y = 2.8%) and by steam distillation, SD, (Y = 0.4%) were quite similar to the SFE extract. The main components of the SFE oil of A. calamus (Y = 3.5%) were acorenone, 13.4%; iso-acorone, 11.6%; (Z)-sesquilavandulol, 11.0%; dehydroxy isocalamendiol, 7.7%; and β-asarone, 5.5%. The comparison w...
