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Ds ,yudi Garnida - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH KONSENTRASI DAN LAMA PERENDAMAN ASAP CAIR TERHADAP KARAKTERISTIK IKAN BAUNG (Mystus nemurus) ASAP KERING
2017Co-Authors: Ikhsan Rizki Sulaiman 12.302.0227, Ds ,willy Pranata Widjaja, Ds ,yudi GarnidaAbstract:ABSTRAK Tujuan dari penelitian ini yaitu, untuk mendapatkan konsentrasi asap cair tempurung kelapa dan lama perendaman yang tepat, terhadap karakteristik ikan baung (Mystus nemurus) asap kering yang dapat diterima oleh konsumen. Rancangan perlakuan pada penelitian ini terdiri dari 2 faktor yaitu konsentrasi asap cair (faktor A) yang terdiri dari 3 taraf yaitu; 2%, 5% dan 8%, dan lama perendaman (faktor B) berturut-turut selama 10 menit, 15 menit dan 20 menit. Rancangan percobaan pada penelitian ini yaitu dengan menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 3x3. Respon penelitian meliputi respon kimia yaitu kadar air dengan metode gravimetri dan kadar protein dengan metode kjehdahl, respon organoleptik (rasa, aroma, tekstur, dan warna) dengan metode uji hedonik serta respon mikrobiologi dengan metode Total Plate Count dan analisis Polycyclic Aromatic Hydrocarbons (PAH) dengan metode HPLC untuk perlakuan terpilih. Hasil Penelitian menunjukan bahwa interaksi antara konsentrasi asap cair dan lama perendaman berpengaruh terhadap kadar air, kadar protein, aroma dan warna. Hasil penelitian menunjukkan perlakuan konsentrasi asap cair 8% dan lama perendaman 20 menit merupakan kombinasi perlakuan terpilih dalam pembuatan ikan baung asap kering dengan kadar air 39,69%, kadar protein 39,96% dan teridentifikasi dua senyawa Polycyclic Aromatic Hydrocarbons (PAH) dengan konsentrasi yang sangat kecil < 0.25 μg/kg. Kata kunci : Ikan Baung (Mystus nemurus), Asap Cair, Lama Perendaman, Ikan Baung Asap Kering
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PENGARUH KONSENTRASI DAN LAM PERENDAMAN ASAP CAIR TERHADAP KARAKTERISTIK IKAN BAUNG (Mystus nemurus) ASAP KERING
2017Co-Authors: Ikhsan Rizki Sulaiman 123020227, Ds ,willy Pranata Widjaja, Ds ,yudi GarnidaAbstract:Tujuan dari penelitian ini yaitu, untuk mendapatkan konsentrasi asap cair dan lama perendaman yang tepat, terhadap karakteristik ikan baung (Mystus nemurus) asap kering yang dapat diterima oleh konsumen. Rancangan perlakuan pada penelitian ini terdiri dari 2 faktor yaitu konsentrasi asap cair (faktor A) yang terdiri dari 3 taraf yaitu; 2%, 5% dan 8%, dan lama perendaman (faktor B) berturut-turut selama 10 menit, 15 menit dan 20 menit. Rancangan percobaan pada penelitian ini yaitu dengan menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 3x3. Respon penelitian meliputi respon kimia yaitu kadar air dengan metode gravimetri dan kadar protein dengan metode kjehdahl, respon organoleptik (rasa, aroma, tekstur, dan warna) dengan metode uji hedonik serta respon mikrobiologi dengan metode Total Plate Count dan analisis Polycyclic Aromatic Hydrocarbons (PAH) dengan metode HPLC untuk perlakuan terpilih. Hasil Penelitian menunjukan bahwa interaksi antara konsentrasi asap cair dan lama perendaman berpengaruh terhadap kadar air, kadar protein, aroma dan warna. Hasil penelitian menunjukkan perlakuan konsentrasi asap cair 8% dan lama perendaman 20 menit merupakan kombinasi perlakuan terpilih dalam pembuatan ikan baung asap kering dengan kadar air 39,69%, kadar protein 39,96% dan teridentifikasi dua senyawa Polycyclic Aromatic Hydrocarbons (PAH) dengan konsentrasi yang sangat kecil < 0.25 μg/kg. Kata kunci : Ikan Baung (Mystus nemurus), Asap Cair, Lama Perendaman, Ikan Baung Asap Kering
Ashok Kumar Verma - One of the best experts on this subject based on the ideXlab platform.
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effect of arsenic on serum biochemical parameters of a fresh water cat fish Mystus vittatus
Social Science Research Network, 2020Co-Authors: Sadguru Prakash, Ashok Kumar VermaAbstract:The present investigation has been designed to study the effect of sub-lethal concentrations (10% and 30%) of heavy metal, arsenic on the serum metabolites, serum enzyme activity and serum electrolytes of Mystus vittatus after exposure to 30 days. The present study shows that serum metabolites such as glucose, protein and bilirubin were significantly decreased while free amino acid, triglyceride and cholesterol were significantly increased; serum enzymes such as acid phosphatase (ACP), transminases (SGPT & SGOT) were increased significantly while alkaline phosphatase (ALP), was decreased significantly; serum electrolytes such as sodium, potassium and calcium were decreased significantly but chloride was increased significantly. Thus, this paper gives an overview of the manipulation of fish, Mystus vittatus as a biomarker of heavy metals through alternation in biochemical parameters.
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impact of arsenic on protein metabolism of a fresh water cat fish Mystus vittatus
Uttar Pradesh Journal of Zoology, 2020Co-Authors: Sadguru Prakash, Ashok Kumar VermaAbstract:The present investigation has been designed to study the effect of sublethal concentrations (10% and 30%) of heavy metal, arsenic on the total protein and amino acid contents in liver and muscles of Mystus vittatus after exposure to 30 days. A significant decreased in protein content and significant increased in amino acids content in liver and muscles of arsenic exposed fishes. A reduction in the protein content and enhancement of free amino acids in the liver and muscles tissues of Mystus vittatus suggests that the tissue protein might have undergone proteolysis, during the stressful situation in the intoxicated fishes to accomplish the impending energy demands. Thus the present study concludes that the protein metabolism of fish Mystus vittatus affected during arsenic exposure and reduces the nutritive value of fish.
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effect of arsenic on enzyme activity of a fresh water cat fish Mystus vittatus
International Journal of Fisheries and Aquatic Studies, 2020Co-Authors: Ashok Kumar Verma, Sadguru PrakashAbstract:The present investigation has been designed to study the effect of sublethal concentrations (10% and 30%) of heavy metal, arsenic on the activity of alkaline and acid phosphatases enzyme in liver and muscles of Mystus vittatus after exposure to 30 days. A significant decreased has been observed in alkaline and acid phosphatases level in liver and muscles of arsenic exposed fish Mystus vittatus. The result of present study obviously indicates that low concentration of arsenic is toxic to fishes and alters the enzymatic activity in different tissues. With the result the entire metabolic activities of fish becomes disturbed. Thus the arsenic is known to affect the biological potential of aquatic animals.
W. Vishwanath - One of the best experts on this subject based on the ideXlab platform.
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FIGURE 4 in Redescription of the striped catfish Mystus carcio (Hamilton) (Siluriformes: Bagridae)
2010Co-Authors: A. Darshan, N. Anganthoibi, W. VishwanathAbstract:FIGURE 4. Comparison of skulls, showing cranial fontanel: a. Mystus carcio, MUMF 9518, 46.6 mm SL; b. Mystus tengara, MUMF 9520, 71.5 mm SL; c. Mystus vittatus, MUMF 9527, 72.7 mm SL; d. Mystus tengara, Unregistered, 42.2 mm SL. e = Supraethmoid, le = lateral ethmoid, f = frontal, af = anterior fontanel, pf = posterior fontanel, eb = epiphysial bar, s = sphenotic, pt = pterotic, so = supraoccipital, ex = exoccipital, op = occipital process
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redescription of the striped catfish Mystus carcio hamilton siluriformes bagridae
Zootaxa, 2010Co-Authors: Achom Darshan, N. Anganthoibi, W. VishwanathAbstract:Pimelodus carcio, originally described in 1822 by Francis Hamilton, has long been treated as a junior synonym of either Mystus tengara or M. vittatus. In this paper we revalidate and redescribe the species and diagnose it from other striped Mystus by the following combination of characters: small adult size, maturing at 44.0 mm standard length; adipose-fin base shorter than or equal to dorsal-fin base; pelvic fin reaching anal-fin origin; pectoral girdle with coracoid shield exposed ventro-laterally below pectoral fin; eyes rounded and large, dorsally orientated on head; vomerine tooth-patch interrupted at midline. The species is distributed in the Ganga-Brahmaputra drainage of northeast India and Bangladesh.
Sadguru Prakash - One of the best experts on this subject based on the ideXlab platform.
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effect of arsenic on serum biochemical parameters of a fresh water cat fish Mystus vittatus
Social Science Research Network, 2020Co-Authors: Sadguru Prakash, Ashok Kumar VermaAbstract:The present investigation has been designed to study the effect of sub-lethal concentrations (10% and 30%) of heavy metal, arsenic on the serum metabolites, serum enzyme activity and serum electrolytes of Mystus vittatus after exposure to 30 days. The present study shows that serum metabolites such as glucose, protein and bilirubin were significantly decreased while free amino acid, triglyceride and cholesterol were significantly increased; serum enzymes such as acid phosphatase (ACP), transminases (SGPT & SGOT) were increased significantly while alkaline phosphatase (ALP), was decreased significantly; serum electrolytes such as sodium, potassium and calcium were decreased significantly but chloride was increased significantly. Thus, this paper gives an overview of the manipulation of fish, Mystus vittatus as a biomarker of heavy metals through alternation in biochemical parameters.
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impact of arsenic on protein metabolism of a fresh water cat fish Mystus vittatus
Uttar Pradesh Journal of Zoology, 2020Co-Authors: Sadguru Prakash, Ashok Kumar VermaAbstract:The present investigation has been designed to study the effect of sublethal concentrations (10% and 30%) of heavy metal, arsenic on the total protein and amino acid contents in liver and muscles of Mystus vittatus after exposure to 30 days. A significant decreased in protein content and significant increased in amino acids content in liver and muscles of arsenic exposed fishes. A reduction in the protein content and enhancement of free amino acids in the liver and muscles tissues of Mystus vittatus suggests that the tissue protein might have undergone proteolysis, during the stressful situation in the intoxicated fishes to accomplish the impending energy demands. Thus the present study concludes that the protein metabolism of fish Mystus vittatus affected during arsenic exposure and reduces the nutritive value of fish.
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effect of arsenic on enzyme activity of a fresh water cat fish Mystus vittatus
International Journal of Fisheries and Aquatic Studies, 2020Co-Authors: Ashok Kumar Verma, Sadguru PrakashAbstract:The present investigation has been designed to study the effect of sublethal concentrations (10% and 30%) of heavy metal, arsenic on the activity of alkaline and acid phosphatases enzyme in liver and muscles of Mystus vittatus after exposure to 30 days. A significant decreased has been observed in alkaline and acid phosphatases level in liver and muscles of arsenic exposed fish Mystus vittatus. The result of present study obviously indicates that low concentration of arsenic is toxic to fishes and alters the enzymatic activity in different tissues. With the result the entire metabolic activities of fish becomes disturbed. Thus the arsenic is known to affect the biological potential of aquatic animals.
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Anomalies in Biochemical Constituents of Kidney in Arsenic induced Mystus vittatus
2020Co-Authors: Sadguru Prakash, Verma, Dr A. K.Abstract:The present investigation has been designed to study the anomalies in biochemical constituents of kidney of Mystus vittatus after exposure to sublethal concentrations (10%, 20% and 30%) for the period of 30 days. The present study, both lower and higher sublethal concentrations of arsenic exposed Mystus vittatus showed a significant decrease in glycogen, protein, triglyceride, acid and alkaline phosphatases content and a significant increase in free amino acid and cholesterol content of its kidney at all periods. The decrease or increase in the contents of these biomolecules levels were more pronounced at 30% concentration and 30 days exposure of arsenic trioxide. Arsenic trioxide may damage the Kidney; inhabit the enzymes activity which causes significant alterations in various metabolic activities
Qiqun Cheng - One of the best experts on this subject based on the ideXlab platform.
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microsatellite dna diversity of coilia Mystus clupeiformes engraulidae in three chinese estuaries
Iranian Journal of Fisheries Sciences, 2012Co-Authors: Fang Chen, Qiqun ChengAbstract:An analysis of eight microsatellite loci in 90 individuals was performed to define the genetic structure and variability of three estuarine populations of Coilia Mystus: 30 individuals each collected from ChangJiang River (Yangtze River) estuary-CJ, MinJiang River estuary-MJ, and ZhuJiang River (Pearl River) estuary-ZJ. The results showed polymorphic information contents (PIC) of 0.78, 0.77, and 0.64 in CJ, MJ, and ZJ, respectively. The mean observed number of alleles (Na) ranged from 7.38 to 11.88. The mean observed and expected heterozygosities ranged from 0.09 to 0.21 and from 0.68 to 0.81, respectively. The ZJ population was least polymorphic. Highly significant deviations from Hardy-Weinberg equilibrium, mostly due to deficits of heterozygote, were found in these three populations. Pairwise FST and Reynolds‟ distance indicated that the three estuarine populations were genetically distinct, in accordance with the principal component analysis (PCA) and the Bayesian model-based clustering algorithm analysis. The results showed that pairwise genetic differentiation among these three estuarine populations were relatively high, with possible divergence to subspecies level. This study provides microsatellite DNA evidence for assessing the genetic distinctness of C. Mystus populations and will benefit fishery resource management and sustainable utilization of C. Mystus.
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development of 12 polymorphic microsatellite markers in coilia ectenes jordan and seale 1905 clupeiformes engraulidae and cross species amplification in coilia Mystus linnaeus 1758
Environmental Biology of Fishes, 2011Co-Authors: Qiqun Cheng, Qingyi ZhangAbstract:In this study, twelve polymorphic microsatellite markers were developed for Coilia ectenes (synonym C. nasus). In a sample of 30 C. ectenes individuals in a Wuhan population from Yangtze River, a total of 78 alleles were detected, and the number of alleles per locus ranged from 3 to 12. The observed and expected heterozygosities ranged from 0.100 to 1.000 and from 0.098 to 0.899, respectively. Six loci significantly deviated from the Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.004), and no significant linkage disequilibrium between pairs of loci was found. The applicability of these markers in a closely related species, C. Mystus, was evaluated by cross-species amplification. These microsatellite markers will be useful in studies on population genetics, conservation genetics, and fishery management and in the construction of genetic linkage maps of C. ectenes and C. Mystus.
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Morphological Variation of Coilia Mystus (Clupeiformes: Engraulidae) in Three Chinese Estuaries
Journal of Life Sciences, 2010Co-Authors: Qiqun ChengAbstract:Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia Mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each individual, and Burnaby's multivariate method was used to obtain size-adjusted shape data. The cluster analysis and discriminant analysis were used to discriminate morphological differences among populations. The results indicated that 1) the three populations were clustered into two distinct groups: the first group included Changjiang C. Mystus and Zhujiang C. Mystus, the last one included Minjiang C. Mystus, and 2) discriminant analysis with selected 5 morphological parameters showed that the identification accuracy was between 98.7952% and 100%, and global identification accuracy was 99.2933%. Reproductive isolation and adaption to environmental condition are determinant factors for morphological variation between populations of C. Mystus.
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a preliminary genetic distinctness of four coilia fishes clupeiformes engraulidae inferred from mitochondrial dna sequences
Russian Journal of Genetics, 2008Co-Authors: Qiqun Cheng, Huaping Cheng, Li Wang, Yang ZhongAbstract:In order to understand the genetic distinctness between four Coilia fishes, i.e. (C. ectenes, C. e. taihuensis, C. Mystus, and C. grayii) the combined cytochrome b (cytb), 12S rRNA and 16S rRNA gene sequences were obtained and analysed. The results are as follows: (1) Neighbour-joining (NJ) and maximum parsimony (MP) trees were built with 1000 bootstrap replicates. They supported that Coilia fishes consisted of two clades: C. grayii form a monophyletic group, and the remaining samples form the other monophyletic group; (2) The topological differences between the NJ tree and the MP tree are checked by Templeton test, indicating that their differences are insignificant (P > 0.05); and (3) Kimura two-parameter distances between the samples of C. ectenes, C. e. taihuensis, and C. Mystus all do not exceed 1.6%, and most of them are between 0 and 0.9%. Coilia ectenes, C. e. taihuensis, and C. Mystus are closely related and their differences may be under subspecies.
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mitochondrial dna diversity of coilia Mystus clupeiformes engraulidae in three chinese estuaries
Environmental Biology of Fishes, 2008Co-Authors: Qiqun Cheng, Huaping Cheng, Qingyi ZhangAbstract:We used sequences of mitochondrial cytb and 16SrRNA gene segments in order to clarify the genetic diversity and population structure in three Chinese estuary populations of Coilia Mystus: 21 individuals from ChangJiang River (Yangtze River) estuary, 22 from MinJiang River estuary, and 22 from ZhuJiang River (Pearl River) estuary (65 individuals total). We obtained 607 base pairs of consensus cytb sequence. Thirty four distinct haplotypes were detected among the 65 cytb sequences. The indexes of nucleotide diversity (π) in these three populations were ChangJiang 0.533%, MinJiang 1.135%, and ZhuJiang 0.268%. MinJiang is the largest of the three populations. Genetic distances within the populations were between 0.3 and 1.2%, and 0.8 to 10.8% among populations. The largest genetic distance was 10.8% between the ChangJiang and ZhuJiang populations, and the smallest was 0.8% between MinJiang and ZhuJiang populations. Analysis of molecular variance (AMOVA) analysis revealed that variation among populations accounts for 90.25% of total variation, suggesting that this is the main source of total variance. We obtained 470 base pairs of consensus sequence of 16SrRNA. We detected 19 distinct haplotypes among the 65 sequences. The indexes of nucleotide diversity (π) in these three populations were ChangJiang 0.108%, MinJiang 0.843%, and ZhuJiang 0.097%. MinJiang is also the largest among these three populations. Genetic distances were between 0.1 and 0.9% within populations and 0.5 to 1.9% between populations. The largest genetic distance was the 1.9% between the ChangJiang and MinJiang populations, and the smallest was 0.5% between the MinJiang and the ZhuJiang populations. AMOVA analysis disclosed that variation among populations accounts for 74.61% of total variation, suggesting that this is the main source of total variation. The results of this study suggest that the three Coilia Mystus populations, especially the most isolated Changjiang population, have developed significant genetic structure.
